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@PoratShliomLab @poratshliomlab.bsky.social · Jun 2

Congrats, Ivy!

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@PoratShliomLab @poratshliomlab.bsky.social · May 20

Congratulations, Ivy!!

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@PoratShliomLab @poratshliomlab.bsky.social · May 9

Milestones is an annual publication covering the exciting research performed at the National Cancer Institute, NIH. This year, my group is honored to be included!

Ivy L. Xiong @ivyxiong.bsky.social · May 9

It's truly a privilege to work with @poratshliomlab.bsky.social at NIH/NCI on understanding metabolic multi-tasking at the tissue level, in the liver and in the kidney.

Congrats to the entire lab for the highlight (on p.20) as well as the stunning cover image! 🎉
ccr.cancer.gov/news/milesto...

CCR Milestones - 2025 | Center for Cancer Research

Every year, CCR makes remarkable contributions to the understanding, detection, treatment and prevention of cancer. This digital-only issue of our annual publication, Milestones, features 13 of our to...

ccr.cancer.gov

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@PoratShliomLab @poratshliomlab.bsky.social · May 9

Thank you @ivyxiong.bsky.social for being part of the team!

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Reposted by @PoratShliomLab

FocalPlane @focalplane.bsky.social · Apr 14

We also host a microscopy database on FocalPlane, which you can use for searching for collaborators, reviewers and speakers.

We'd like to invite all members of the community to join and use the Network.
focalplane.biologists.com/network/

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Reposted by @PoratShliomLab

bioRxivpreprint @biorxivpreprint.bsky.social · Apr 14

Single cell spatial proteomics maps human liver zonation patterns and their vulnerability to fibrosis https://www.biorxiv.org/content/10.1101/2025.04.13.648568v1

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Reposted by @PoratShliomLab

Caroline Weiss @carolineweiss.bsky.social · Apr 14

Preprint alert ‼️

Excited to share our work investigating liver zonation in human health and disease with our advanced single-cell Deep Visual Peoteomics framework!

Mann Lab @mannlab.bsky.social · Apr 14

Introducing our single-cell DVP framework combining strategic cell selection with protein gradient mapping algorithms. Analyzing hepatocyte proteomes across 18 human livers, revealing cross-species differences, and mapping disruptions in liver fibrosis.
www.biorxiv.org/content/10.1...

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@PoratShliomLab @poratshliomlab.bsky.social · Apr 14

Is liver zonation conserved from mice to humans? And how does fibrosis affect zonation in patients? Now, these questions are answered using scDVP. Some collaborations are meant to be! @carolineweiss.bsky.social@frosenberger.bsky.social @mannlab.bsky.social@mlsb-borgwardt.bsky.social

Mann Lab @mannlab.bsky.social · Apr 14

Introducing our single-cell DVP framework combining strategic cell selection with protein gradient mapping algorithms. Analyzing hepatocyte proteomes across 18 human livers, revealing cross-species differences, and mapping disruptions in liver fibrosis.
www.biorxiv.org/content/10.1...

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Reposted by @PoratShliomLab

Sarah Cohen @cohenlaboratory.bsky.social · Dec 21

Check out this preprint from collaborator Lance Johnson @johnsonlab.bsky.social and team characterizing the APOE3 vs E4 #LipidDroplet -ome! Some interesting Easter eggs in there 🐇🥚😉

Lance Johnson @johnsonlab.bsky.social · Dec 21

new preprint!

www.biorxiv.org/content/10.1...

a characterization of APOE effects on the lipid droplet-ome (turns out you see a lot of "AD-related" proteins!)

first authors Cassi Friday & Isaiah Stephens + great collaborators Scott Gordon, @cohenlaboratory.bsky.social @morganti.bsky.social

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Reposted by @PoratShliomLab

Jonny Nixon-Abell @abelljonny.bsky.social · Dec 13

Very cool!

@PoratShliomLab @poratshliomlab.bsky.social · Dec 12

Preprint alert!! How zonated hepatocytes adapt to changes in lipid influx due to fasting or a Western diet (WD). We developed a microscopy-based phenotyping method called single-cell phenomics (scPhenomics) to measure the features of mitochondria and lipid droplets (LDs).

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 13

Thank you, Jonny!

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Reposted by @PoratShliomLab

André Nadler @nadlerlab.bsky.social · Dec 13

Beautiful work - I love the analysis of organelle rearrangement in response to changes in lipid flux.

@PoratShliomLab @poratshliomlab.bsky.social · Dec 12

Preprint alert!! How zonated hepatocytes adapt to changes in lipid influx due to fasting or a Western diet (WD). We developed a microscopy-based phenotyping method called single-cell phenomics (scPhenomics) to measure the features of mitochondria and lipid droplets (LDs).

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 13

Thank you!

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 13

Fits nicely with what you showed in brown adipose.

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 13

Thank you, Marc!

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 13

Thank you, Doug!

Reposted by @PoratShliomLab

Sarah Cohen @cohenlaboratory.bsky.social · Dec 12

Wow!! Amazing preprint from the Porat-Shliom lab showing how organelle signatures vary across space and diet in the liver. This paper has it all: gorgeous microscopy, lipid droplets, mitochondria, and one of my favorite proteins, PLIN5 🤩

@PoratShliomLab @poratshliomlab.bsky.social · Dec 12

Preprint alert!! How zonated hepatocytes adapt to changes in lipid influx due to fasting or a Western diet (WD). We developed a microscopy-based phenotyping method called single-cell phenomics (scPhenomics) to measure the features of mitochondria and lipid droplets (LDs).

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 13

Thank you, Sarah! Your insights into PLIN5 functions were invaluable!

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Reposted by @PoratShliomLab

bioRxiv Cell Biology @biorxiv-cellbio.bsky.social · Dec 12

Spatially resolved rewiring of mitochondria-lipid droplet interactions in hepatic lipid homeostasis. https://www.biorxiv.org/content/10.1101/2024.12.10.627730v1

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 12

Fantastic teamwork in the Porat-Shliom lab AND the amazing resources at the IRP at NCI!

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 12

10. In WD conditions, increasing PLIN5 levels enables hepatocytes to regulate the proportion of mitochondria bound to LDs versus those free in the cytosol, facilitating more efficient management of lipid overload.

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 12

9. This could explain how, under nutritional deficits, mito simultaneously supports lipid oxidation for ketogenesis while promoting TG synthesis and storage to mitigate lipotoxicity. READ ON...

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 12

8. During nutritional stress like fasting, the spatial division is lost, in part due to the upregulation of PLIN5 and its phosphorylation status, leading to the emergence of both types of mitochondria in most hepatocytes. READ ON...

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 12

7. Our model: in homeostasis, two types of mitochondria operate simultaneously: periportal mitochondria with a round morphology and a preference for fatty acid oxidation and pericentral mitochondria that synthesize TGs and store them through contact with LDs. more coming...

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@PoratShliomLab @poratshliomlab.bsky.social · Dec 12

6. Can introducing mito-LD contacts in WD reduce toxicity? YES! OE of PLIN5 increased mito-LD interactions, improved redox status, and reduced steatosis—highlighting the physiological significance of organelle interactions in lipid homeostasis and tissue health.

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