Rezwan Siddiquee
@rezwansiddiquee.bsky.social
420 followers 1.2K following 7 posts
PhD Candidate in Synthetic Biology #seekRNA #proteins @Sydney_Uni. Other half of @taylorszyszka https://scholar.google.com/citations?hl=en&user=nZ8TDHcAAAAJ
Posts Media Videos Starter Packs
Pinned
Reposted by Rezwan Siddiquee
arccoesb.bsky.social
Congrats to @taylorszyszka.bsky.social and @rezwansiddiquee.bsky.social who have been invited to Raise the Bar! 20 academics will visit 10 bars across Sydney to talk about their research, for 1 night only.

📅 Thu 3 April, 7:30 PM
📍Bank Hotel, 324 King St, Newtown
🎟️ Register: bit.ly/43Ci7n0

#RTBSYD
rezwansiddiquee.bsky.social
Really proud of the famous Taylor Szyszka for the perseverance for this project! Amazing work!
lorneproteins.bsky.social
Light-ning Talks #LorneProteins2025 Taylor Szyszka Engineering carbon-fixing protein cages. Carboxysomes are great, but encapsulins can assemble a uniform cage around any target tagged with a cargo peptide at C term. 42 nm across. Carboxysome mimic with encapsulin? Encapsulating rubisco.
Taylor N Szyszka standing behind a lectern giving a cool talk about building encapsulated rubisco complexes.

Co-expressed with encapsulin - no activity. But is rubisco assembled? Staged induction expression system. 2 plasmids; IPTG for rubisco; rhamnose for encapsulin with fresh media.

Staged expression retains activity.
rezwansiddiquee.bsky.social
Had such a wonderful time at #Coesb2024! Got to meet like minded synthetic biologists and learn about their successes and challenges. And huge thanks to @ARC_CoESB for an award for Outreach Excellence!
Reposted by Rezwan Siddiquee
verenaresch.bsky.social
I sometimes forget how flexible proteins are. It becomes really apparent when you use NMR states for an animation. That poor cofactor is getting pushed around a lot 😅

#sciart #blender3d #biocatalysis
rezwansiddiquee.bsky.social
Check out Yu Heng’s post below.
bsky.app/profile/yuhe...
yuhenglau.bsky.social
Long time lurker, first time poster... announcing our latest preprint on encapsulin protein cages!

We can now assemble them beautifully in vitro, without the ugly defects you get using acid/base or denaturants.
This lets us put any synthetic cargo into these cages.

www.biorxiv.org/content/10.1...
www.biorxiv.org
rezwansiddiquee.bsky.social
This work is led by my better half, the superstar @taylorszyszka.bsky.social and is our first paper together! Many thanks to our supervisor @yuhenglau.bsky.social for incredible support and of course, from contributors Alex, Lachlan, Regi, Titus and collaborators Andrew and Claire from UTS.
rezwansiddiquee.bsky.social
How did we do this? A clean system of fusing a steric block to encapsulin protein cage monomer – prevents assembly. Take this in vitro, add *any* cargo to the reaction and cleave off the block. And voila! Assembled in vitro cages that are surprisingly better in every way.
Reposted by Rezwan Siddiquee
yuhenglau.bsky.social
Long time lurker, first time poster... announcing our latest preprint on encapsulin protein cages!

We can now assemble them beautifully in vitro, without the ugly defects you get using acid/base or denaturants.
This lets us put any synthetic cargo into these cages.

www.biorxiv.org/content/10.1...
www.biorxiv.org