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Sara McArdle @saramcardle.bsky.social · 9h

I want to thank all of the co-authors, though I can't find any of them are on bluesky. In particular, I want to thank Priyanka Saminathan for all her work getting this to the finish line!

Sara McArdle @saramcardle.bsky.social · 9h

All of the segmentation and morphometric analysis was done with the open-source platform QuPath. The scripts I wrote are on Github and the raw images are on the BioImage Archive, because open-source is the way to go!

But seriously, look at these cells! They are so angry.

Reactive microglia looking particularly angry. Iba1 showing cell shape in magenta, large amounts of CD68 in green, nuclei in blue. The cells are outlined in brown and the nuclei are outlined in cyan. There are lines connecting the nuclear centroids from Delaunay triangulation.

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Sara McArdle @saramcardle.bsky.social · 9h

We used widefield slidescanning + deconvolution + Z projection to image microglia in the hippocampus. 2 years in with ~11000 cell analyzed and I'm still not over how pretty microglia can be.

We found signs that glia in female AD mice begin to react to *something* before there are even plaques.

Microscopy image of a pink microglia with blue nuclei and focal points of green. Magenta = Iba1; Green = CD68; Blue = Hoechst.

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Sara McArdle @saramcardle.bsky.social · 9h

I'm thrilled to share that our work studying microglia morphological changes in early Alzheimer's in mice is up!
www.biorxiv.org/content/10.1...

Unmasking Early Microglial Remodeling in an Alzheimer's Disease Mouse Model

Early neuroimmune remodeling is a critical yet understudied component of Alzheimer's disease (AD) pathogenesis. To investigate microglial contributions to AD development prior to overt plaque depositi...

www.biorxiv.org

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Reposted by Sara McArdle

Merriam-Webster @merriam-webster.com · 7d

We are thrilled to announce that our NEW Large Language Model will be released on 11.18.25.

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Reposted by Sara McArdle

Anna Bower @annabower.bsky.social · 10d

While I’m at it: You guys know what to do!!

youtu.be/jPCJIB1f7jk?...

Lip Sync Battle - Tom Holland

YouTube video by Comedy Central Latinoamérica

youtu.be

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Sara McArdle @saramcardle.bsky.social · Sep 5

Happy Fluorescence Friday!

Rainbow Z projection of lymph node follicular dendritic cells.

A psychedelic-looking image of what a network of cells (follicular dendritic cells in a lymph node).

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Reposted by Sara McArdle

A/Prof Sam Stehbens PhD (She/Her) @samstehbens.bsky.social · Sep 4

Who say what now?!! BLUEY THE MOVIE?!! #bluey #myChildWillBeTooOldButIllStillGo

www.bluey.tv/blog/date-an...

two cartoon animals hugging each other with the word seduff on the bottom right

ALT: two cartoon animals hugging each other with the word seduff on the bottom right

media.tenor.com

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Sara McArdle @saramcardle.bsky.social · Aug 8

Chaotic good! I honestly spent 5 minutes debating whether hdf5 or ome.zarr was more chaotic

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Sara McArdle @saramcardle.bsky.social · Aug 8

True!

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Sara McArdle @saramcardle.bsky.social · Aug 8

Yea, true. But I'm not sure it's more evil than the other 3.

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Sara McArdle @saramcardle.bsky.social · Aug 7

I got this in my head and I couldn't focus on work until it existed.

Microscopy image file alignment chart, based on how I feel if you give me an image to analyze:

D&D style alignment chart for microscopy images.

Lawful good: .ome.tiff
Lawful neutral: .png
Lawful evil: .jpg
Neutral Good: .tiff
Neutral: .pdf
Neutral Evil: .mrxs
Chaotic Good: .h5
Chaotic Neutral: .ppt
Chaortic Evil: .jpg.avi.tiff

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Reposted by Sara McArdle

Stefania Marcotti @lankylaste.bsky.social · Aug 6

Ready to automate your #microscopy #imageanalysis tasks? Applications are now open for our next "Introduction to Image Analysis" training course, in London at the @crick.ac.uk on October 27th-28th, 2025! 🔬💻

More info ⬇️
www.crick.ac.uk/whats-on/int...

Introduction to image analysis

www.crick.ac.uk

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Sara McArdle @saramcardle.bsky.social · Jul 21

Napari

Sara McArdle @saramcardle.bsky.social · Jul 8

Yes!
imagej.net/software/nih...

NIH Image

The ImageJ wiki is a community-edited knowledge base on topics relating to ImageJ, a public domain program for processing and analyzing scientific images, and its ecosystem of derivatives and variants...

imagej.net

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Sara McArdle @saramcardle.bsky.social · Jul 7

The original NIH Image from 1987 recommended 4 MB of RAM!

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Reposted by Sara McArdle

Morgan Carter @morgancarterphd.bsky.social · Jun 25

It's not just #NIH. It's not just #NSF. It's not just #NASA.

The whole scientific community has to make clear that all science across fed agencies is being destroyed in the US.

We can't just ask for our particular piece of the pie. The whole pie is being thrown out - saving your bit won't work.

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Reposted by Sara McArdle

Tim Onion @bencollins.bsky.social · Jun 11

Every flashbang could instead be a meal for a poor kid. Every hotel room for a National Guardsman deployed to fire at our neighbors could house a homeless vet. Every "less-than-lethal" bullet pulled from a journalist's eye could be already needed healthcare for anyone else. All of this is a choice.

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Reposted by Sara McArdle

Abby Polter @ampolter.bsky.social · May 15

It's not that we might lose our jobs. It's that all of it will disappear or mutate beyond recognition. I worry we're not talking or thinking enough about this deeper sadness hiding behind our immediate anxieties and it's this heaviness that has us all at a breaking point.

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Reposted by Sara McArdle

Steve Lieber: NYCC K-19 @stevelieberart.bsky.social · May 7

Cleansing the timeline with video from the Cincinnati Zoo & Botanical Garden.

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Reposted by Sara McArdle

BioVoxxel (Jan Brocher) @biovoxxel.de · Apr 23

🚀 Tired of manual image analysis or scared of scripting?

Our self-paced “ImageJ Macro Scripting for Automatic Image Analysis” course makes automation easy—no coding experience needed!

Start from scratch & build pro-level macros.

www.biovoxxel.de/individual-w...

#ImageJ #MacroScripting

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Reposted by Sara McArdle

Beth Cimini 🔬💻📊 @bethcimini.bsky.social · Apr 8

Bioimaging and bioimage analysis friends, some help if you have 2-3 mins; for a paper, we're trying to estimate the number of biologists trained in open source software or tools in the last ~5 years. (1/2) #bioimaging #microscopy #bioimageanalysis

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Reposted by Sara McArdle

ABC 10News @abc10news.bsky.social · Apr 14

WATCH: Elephants at the San Diego Zoo Safari Park react to Monday's 5.2 magnitude earthquake that shook San Diego County. The elephants formed an "alert circle" meant to protect the young and the entire herd from any threats, according to the San Diego Zoo Wildlife Alliance.

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Reposted by Sara McArdle

Rita Strack @ritastrack.bsky.social · Apr 10

Your yearly reminder to acknowledge the core facilities you use and their staff scientists in your papers. These scientists are a crucial part of the scientific ecosystem and to continue to exist they need tangible credit for their work. Plus their associated expertise adds credibility to your work.

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Reposted by Sara McArdle

Beth Cimini 🔬💻📊 @bethcimini.bsky.social · Apr 8

If you train people in ImageJ, Fiji, whatever, even if it isn't your day job, please let us know, and please share this post! Thanks a ton, we really appreciate it. (2/2) forms.gle/C8dUpg8qZkbD... #bioimaging #microscopy #bioimageanalysis

Open Source Bioimage Analysis Training Survey

We are attempting for a publication to estimate the number of biologists who have attended a tutorial or training course in open source bioimage analysis (especially user friendly tools, but anything ...

forms.gle

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