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sdelcilab.bsky.social
Sdelci Lab
@sdelcilab.bsky.social
940 followers 1.2K following 36 posts
The Epigenetic Face of Cancer Metabolism Lab We study NUCLEAR METABOLISM at CRG, Barcelona https://sdelcilab.crg.eu/
sdelcilab.crg.eu
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sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · 7d
Dr. Jane Goodall has passed away. Her groundbreaking work with chimpanzees and her lifelong dedication to conservation changed how we see and care for our planet.
science.org
Science Magazine @science.org · 7d
Jane Goodall has died at the age of 91.

In 2020, Goodall spoke with Science Editor-in-Chief H. Holden Thorp for a #ScienceEditorial reflecting on the 60th anniversary of her arrival in Gombe to study wild chimpanzees. Read more: https://scim.ag/472Chb8
"… Goodall’s … is a story of genuine scientific curiosity, determination, and respect for nature and humanity …" H. Holden Thorp, Editor-in-chief, Science journals
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Reposted by Sdelci Lab
arnausebe.bsky.social
Arnau Sebé-Pedrós @arnausebe.bsky.social · 14d
Happy to share the Biodiversity Cell Atlas white paper, out today in @nature.com. We look at the possibilities, challenges, and potential impacts of molecularly mapping cells across the tree of life.
www.nature.com/articles/s41...
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Reposted by Sdelci Lab
wkhuber.bsky.social
Wolfgang Huber @wkhuber.bsky.social · 22d
Science: only when you write up the manuscript, you realize what you should have done.
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sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Sep 7
Being open to new ideas: Metabolic Enzymes in the Nucleus as Chromatin Instructors?

Merging
#chromatin
#epigenetics
#metabolism
To understand new cellular rules.
itaiyanai.bsky.social
Itai Yanai @itaiyanai.bsky.social · May 31
90% of doing science is being open to new ideas.
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sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jul 30
Uncovering how mechanical forces can influence nuclear ATP levels, with downstream effects on DNA damage response and cell cycle progression.

#Mechanobiology #NuclearMetabolism #CellFate #DNArepair #CellCycle
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sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jul 30
🚀 We’re excited to share our latest publication in collaboration with Verena Ruprecht's group at @crg.eu

Mechanobiology meets Nuclear Metabolism

www.nature.com/articles/s41...
Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects - Nature Communications
The authors uncover a mechano-metabolic adaptation where confinement induces rapid mitochondrial relocalization to the nuclear periphery, generating localized nuclear ATP surges that support chromatin...
www.nature.com
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sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · May 30
Fantastic to see this wonderful piece out! Happy to have contributed to this effort 💪
ksdrew.bsky.social
Kevin Drew @ksdrew.bsky.social · May 29
Very excited that our work describing hu.MAP3.0 is published in @molsystbiol.org. Here we use machine learning to integrate >25k mass spectrometry experiments to place ~70% of human proteins into 15k protein complexes.

www.embopress.org/doi/full/10....
hu.MAP3.0: atlas of human protein complexes by integration of >25,000 proteomic experiments | Molecular Systems Biology
imageimagehu.MAP3.0 integrates mass spectrometry experiments to identify human protein complexes. Using this resource, this study characterizes covariation of complexes, identifies mutually exclusive ...
www.embopress.org
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Reposted by Sdelci Lab
sandradcastillo.bsky.social
Sandra Castillo @sandradcastillo.bsky.social · Dec 18
🔬 ¡Buscamos Técnico/a de laboratorio!
📋 Mantenimiento de colonia, procesamiento de muestras y más.
📍 Barcelona
📩+info: hospitalsantjoandedeu.talentclue.com/es/node/1129...

Please RT!
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sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
BIG THANKS to everyone involved, especially Toni for his amazing work!

Thanks to our sponsors:
@ageinves.bsky.social
@ERC_Research #ERCStG EPICAMENTE
@crg.eu
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
Open questions:
Is nuclear metabolism linked to #aging?
Can we reverse the aging process by restoring nuclear metabolism?
What else besides PIP2?

If you are interested in answering these questions, please contact us!
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sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
Our study links nuclear PIP2 metabolism to nucleoli structure, histone modification, and cell cycle progression. This provides evidence that nuclear metabolism oscillations can control cell cycle progression, which, when altered, can induce cell cycle arrest and cell senescence.
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sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
4) The aberrant localization of PIP2 in the nucleus/nucleoli and the alteration of the nucleoli structure lead to changes in the accumulation of histone marks. For example, H4K20me1 decreases, which subsequently affects proper cell cycle progression.
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
3) What happens to cells when we interfere with PIP2 metabolism in the nucleus? In addition to preventing cell cycle progression, the structure of the nucleolus is affected and the localization of PIP2 to the Fibrillarin-labeled nucleoli region is altered.
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
2) We used our lab's gold standard chromatome protocol to study the oscillation of metabolic enzymes on chromatin during the cell cycle. We identified a correlation between cell cycle progression and PIP2 metabolism on chromatin.
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
1) By integrating FUCCI reporters and classical proliferation/senescent markers, we identified a population of cells, which we termed G0-senescent, that had never before been identified using FUCCI.
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
Our initial question was: does the cell cycle control the association of metabolic enzymes with chromatin? The short answer is YES!
We adapted the FUCCI system and extensively characterized U2OS FUCCI using HT-microscopy and FACS.
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
As promised, here is our NEW preprint on CELL CYCLE ASSOCIATED PIP2 NUCLEAR METABOLISM by our SUPER postdoc Toni Gañez, who knows all the secrets of FUCCI reporters 👇
www.biorxiv.org/content/10.1...
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sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
Open questions:
Is nuclear metabolism linked to aging?
Can we reverse the aging process by restoring nuclear metabolism?
What else besides PIP2?

If you are interested in answering these questions, please contact us!
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
Our study links nuclear PIP2 metabolism to nucleoli structure, histone modification, and cell cycle progression. This provides evidence that nuclear metabolism oscillations can control cell cycle progression, which, when altered, can induce cell cycle arrest and cell senescence.
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
4) The aberrant localization of PIP2 in the nucleus/nucleoli and the alteration of the nucleoli structure lead to changes in the accumulation of histone marks. For example, H4K20me1 decreases, which subsequently affects proper cell cycle progression.
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
3) What happens to cells when we interfere with PIP2 metabolism in the nucleus? In addition to preventing cell cycle progression, the structure of the nucleolus is affected and the localization of PIP2 to the Fibrillarin-labeled nucleoli region is altered.
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
2) We used our lab's gold standard chromatome protocol to study the oscillation of metabolic enzymes on chromatin during the cell cycle. We identified a correlation between cell cycle progression and PIP2 metabolism on chromatin.
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
We adapted the FUCCI system and extensively characterized U2OS FUCCI using microscopy and FACS.
1) By integrating FUCCI reporters and classical proliferation/senescent markers, we identified a population of cells, which we termed G0-senescent, that had never before been identified using FUCCI.
1 1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Jan 3
Our initial question was: does the cell cycle control the association of metabolic enzymes with chromatin? The short answer is YES!
1
sdelcilab.bsky.social
Sdelci Lab @sdelcilab.bsky.social · Dec 24
Merry Christmas from the Sdelci Lab!

Stay tuned for our last preprint before the end of the year... lots of FUCCI colors 🎄-style and metabolic enzymes on chromatin during cell cycle progression
COMING SOON 💥
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