We have studied the copper sites in three cryogenic electron-microscopy structures of particulate methane monooxygenase with quantum refinement. We show that the CuA, CuC and CuD sites are correctly modelled and that the CuB site is mononuclear in all structures, whereas we find no support for copper in the suggested trinuclear active site or two sites in the so-called copper sponge in PDB entry 7ev9.

The acquisition of iron is critical for the survival and the virulence of numerous infectious pathogens, and most bacteria acquire ferrous iron (Fe2+) by utilizing the ferrous iron transport (Feo) sys...

The solicitation for the 2025 submission dates for the NSF Graduate Research Fellowship Program is out, with the first deadline of November 10 for life-sciences disciplines. The big news is that el…

Replaying history with less NIH funding shows widespread impacts on drug-linked research

The cytosolic iron–sulfur cluster assembly (CIA) targeting complex maturates over 30 cytosolic and nuclear Fe–S proteins, raising the question of how a single complex recognizes such a diverse set of ...

Increasing brain complexity is a major step in the evolution of species. Here, we show that, in the transition from amphibians to reptiles, the DNA re…

The transition metal copper (Cu) is biologically essential across all three domains of life. Several Cu-dependent proteins and enzymes produced by the Gram-positive bacterium Bacillus subtilis have…

N-terminal proteoforms are protein variants with altered N termini that arise from RNA-driven processes, such as alternative promoter usage, splicing,…

Lipids are essential metabolites that play critical roles in multiple cellular pathways. Like many primary metabolites, mutations that disrupt lipid synthesis can be lethal. Proteins involved in lipid...

Florida will move to end all vaccine mandates in the state, Florida Surgeon General Dr. Joseph Ladapo announced Wednesday.