Spirochrome
@spirochrome.com
Our main focus is to offer innovative tools for live cell fluorescence imaging to the scientific community.
https://spirochrome.com/
https://spirochrome.com/
Are you staining DNA in your ExM samples?
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
January 8, 2026 at 2:25 PM
Are you staining DNA in your ExM samples?
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well!
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
January 5, 2026 at 12:54 PM
Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well!
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
A new article by @zhixingchen2.bsky.social shows that the far red mitochondrial probe PKmito Deep Red is a robust mitochondrial membrane potential probe when imaged by FLIM.
www.nature.com/articles/s41...
PKmito Deep Red is exclusively available at Spirochrome:
spirochrome.com/product/pkmi...
www.nature.com/articles/s41...
PKmito Deep Red is exclusively available at Spirochrome:
spirochrome.com/product/pkmi...
December 16, 2025 at 12:39 PM
A new article by @zhixingchen2.bsky.social shows that the far red mitochondrial probe PKmito Deep Red is a robust mitochondrial membrane potential probe when imaged by FLIM.
www.nature.com/articles/s41...
PKmito Deep Red is exclusively available at Spirochrome:
spirochrome.com/product/pkmi...
www.nature.com/articles/s41...
PKmito Deep Red is exclusively available at Spirochrome:
spirochrome.com/product/pkmi...
The images in the main text are also definitely worth a look!
November 24, 2025 at 1:34 PM
The images in the main text are also definitely worth a look!
A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
November 24, 2025 at 1:34 PM
A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
October 29, 2025 at 9:30 AM
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
October 29, 2025 at 9:30 AM
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
October 2, 2025 at 8:40 AM
📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
September 19, 2025 at 9:45 AM
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
HAK-actin™ was developed in the labs of @centriolelab.bsky.social and @dudinlab.bsky.social.
Find out more about HAK-actin™ in the BioRxiv manuscript: doi.org/10.1101/2025...
We are grateful to @dudinlab.bsky.social for providing the creolimax frangantissima data used in the movie
Find out more about HAK-actin™ in the BioRxiv manuscript: doi.org/10.1101/2025...
We are grateful to @dudinlab.bsky.social for providing the creolimax frangantissima data used in the movie
September 10, 2025 at 7:58 AM
HAK-actin™ was developed in the labs of @centriolelab.bsky.social and @dudinlab.bsky.social.
Find out more about HAK-actin™ in the BioRxiv manuscript: doi.org/10.1101/2025...
We are grateful to @dudinlab.bsky.social for providing the creolimax frangantissima data used in the movie
Find out more about HAK-actin™ in the BioRxiv manuscript: doi.org/10.1101/2025...
We are grateful to @dudinlab.bsky.social for providing the creolimax frangantissima data used in the movie
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
September 10, 2025 at 7:58 AM
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
The video was adapted from movie 3 in the accepted manuscript "Piezo1 balances membrane and cortex tension to stabilize intercellular junctions and maintain epithelial barrier " by @sarawickstrom.bsky.social
doi.org/10.1242/jcs....
The article also features our membrane tension probe Flipper-TR!
doi.org/10.1242/jcs....
The article also features our membrane tension probe Flipper-TR!
August 5, 2025 at 2:09 PM
The video was adapted from movie 3 in the accepted manuscript "Piezo1 balances membrane and cortex tension to stabilize intercellular junctions and maintain epithelial barrier " by @sarawickstrom.bsky.social
doi.org/10.1242/jcs....
The article also features our membrane tension probe Flipper-TR!
doi.org/10.1242/jcs....
The article also features our membrane tension probe Flipper-TR!
Our Live cell actin probe SPY650-FastAct efficiently labels primary keratinocytes isolated from mice.
August 5, 2025 at 2:09 PM
Our Live cell actin probe SPY650-FastAct efficiently labels primary keratinocytes isolated from mice.
🌑Goodbye bleaching, hello brightness! ☀️
We're thrilled to introduce two new Halotag ligands featuring @zhixingchen2.bsky.social fantastic photoresistant BD-dyes!
✅ Highly photoresistant, no photoblueing
✅ Outstanding brightness
✅ Cell permeable
✅ Outperform best in class fluorophores
We're thrilled to introduce two new Halotag ligands featuring @zhixingchen2.bsky.social fantastic photoresistant BD-dyes!
✅ Highly photoresistant, no photoblueing
✅ Outstanding brightness
✅ Cell permeable
✅ Outperform best in class fluorophores
July 16, 2025 at 8:24 AM
🌑Goodbye bleaching, hello brightness! ☀️
We're thrilled to introduce two new Halotag ligands featuring @zhixingchen2.bsky.social fantastic photoresistant BD-dyes!
✅ Highly photoresistant, no photoblueing
✅ Outstanding brightness
✅ Cell permeable
✅ Outperform best in class fluorophores
We're thrilled to introduce two new Halotag ligands featuring @zhixingchen2.bsky.social fantastic photoresistant BD-dyes!
✅ Highly photoresistant, no photoblueing
✅ Outstanding brightness
✅ Cell permeable
✅ Outperform best in class fluorophores
The first UK Peroxisome Network Meeting in Exeter was a great success!
We're grateful to the organizers for giving us the opportunity to support this great event.
Triana Amen presented her amazing work on the development of Peroxi_SPY probes for peroxisome imaging.
Rendez-vous at the next edition!
We're grateful to the organizers for giving us the opportunity to support this great event.
Triana Amen presented her amazing work on the development of Peroxi_SPY probes for peroxisome imaging.
Rendez-vous at the next edition!
July 15, 2025 at 11:26 AM
The first UK Peroxisome Network Meeting in Exeter was a great success!
We're grateful to the organizers for giving us the opportunity to support this great event.
Triana Amen presented her amazing work on the development of Peroxi_SPY probes for peroxisome imaging.
Rendez-vous at the next edition!
We're grateful to the organizers for giving us the opportunity to support this great event.
Triana Amen presented her amazing work on the development of Peroxi_SPY probes for peroxisome imaging.
Rendez-vous at the next edition!
Our mechanosensitive Flipper-TR™ probes at work in a stunning article by Z. Chen Et al. from the University of western Australia.
The study unveils a PM-ER mechanotransduction mechanism in response to external strain that dose-dependently regulates cellular metabolism.
doi.org/10.1126/scia...
The study unveils a PM-ER mechanotransduction mechanism in response to external strain that dose-dependently regulates cellular metabolism.
doi.org/10.1126/scia...
July 1, 2025 at 12:17 PM
Our mechanosensitive Flipper-TR™ probes at work in a stunning article by Z. Chen Et al. from the University of western Australia.
The study unveils a PM-ER mechanotransduction mechanism in response to external strain that dose-dependently regulates cellular metabolism.
doi.org/10.1126/scia...
The study unveils a PM-ER mechanotransduction mechanism in response to external strain that dose-dependently regulates cellular metabolism.
doi.org/10.1126/scia...
Dear Yeast friends: Did you know PKmito ORANGE labels nicely yeast cells mitochondria!
The image is from in a recent article by @fmcbenning.bsky.social : doi.org/10.1002/pro....
PKmito ORANGE is a gentle probe for mitochondria imaging with reduced phototoxicity
spirochrome.com/product/pkmi...
The image is from in a recent article by @fmcbenning.bsky.social : doi.org/10.1002/pro....
PKmito ORANGE is a gentle probe for mitochondria imaging with reduced phototoxicity
spirochrome.com/product/pkmi...
June 27, 2025 at 9:04 AM
Dear Yeast friends: Did you know PKmito ORANGE labels nicely yeast cells mitochondria!
The image is from in a recent article by @fmcbenning.bsky.social : doi.org/10.1002/pro....
PKmito ORANGE is a gentle probe for mitochondria imaging with reduced phototoxicity
spirochrome.com/product/pkmi...
The image is from in a recent article by @fmcbenning.bsky.social : doi.org/10.1002/pro....
PKmito ORANGE is a gentle probe for mitochondria imaging with reduced phototoxicity
spirochrome.com/product/pkmi...
If you are interested in the context of this movie you can read the Science article by @loxstoplox.bsky.social about the role of actin in early embryo spindle formation and look at the other 😍 microscopy images and movies.
doi.org/10.1126/scie...
doi.org/10.1126/scie...
June 23, 2025 at 10:54 AM
If you are interested in the context of this movie you can read the Science article by @loxstoplox.bsky.social about the role of actin in early embryo spindle formation and look at the other 😍 microscopy images and movies.
doi.org/10.1126/scie...
doi.org/10.1126/scie...
Let's start the week with this movie kindly shared by @loxstoplox.bsky.social
The movie shows a mouse embryo labeled with Formin-2-GFP and SPY650-DNA during nuclear envelope breakdown. Note the nuclear dilution of Formin-2-GFP immediately before the SPY650-DNA labeled chromosomes start compaction.
The movie shows a mouse embryo labeled with Formin-2-GFP and SPY650-DNA during nuclear envelope breakdown. Note the nuclear dilution of Formin-2-GFP immediately before the SPY650-DNA labeled chromosomes start compaction.
June 23, 2025 at 10:54 AM
Let's start the week with this movie kindly shared by @loxstoplox.bsky.social
The movie shows a mouse embryo labeled with Formin-2-GFP and SPY650-DNA during nuclear envelope breakdown. Note the nuclear dilution of Formin-2-GFP immediately before the SPY650-DNA labeled chromosomes start compaction.
The movie shows a mouse embryo labeled with Formin-2-GFP and SPY650-DNA during nuclear envelope breakdown. Note the nuclear dilution of Formin-2-GFP immediately before the SPY650-DNA labeled chromosomes start compaction.
New preprint by @kjohnsson.bsky.social lab!
A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing.
The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.
A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing.
The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.
![Live-cell confocal imaging of histone H2B type-2E-Hpep11 CRISPR KI cell lines after 2-hours labeling with the specified CA- ligand [100 nM]. Images were taken with optimal image acquisition parameter for each dye. Scale bar: 50 μm.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:uf4m7zlmcjlg2biwn4s2k32y/bafkreibozn5fz6sgeksqrz3vmy6uiydtnvwmb5my7r3xecko2erokryn3a@jpeg)
![(a) Confocal laser scanning microscopy (CLSM) and STED images of mitochondria in U2OS cells coexpressing cpHaloΔ3 and TOM20-Hpep, either overexpressed or endogenously tagged. Scale bar: 1 μm. Pixel intensities scaled according to reference bar. (b) Representative CLSM, STED images of the CRISPR/Cas9 KI cells expressing TOM20 tagged with intact HaloTag (upper) or Hpep11 (bottom). (c) Intensity profiles along mitochondrial tubules (red and blue lines in b). Scale bar: 2 μm. (d) Representative CLSM and STED images of endogenous Hpep11-tagged clathrin with cpHaloΔ3 coexpression. Scale bar: 10 μm (overview) and 2 μm (magnification). (e) Representative CLSM, STED
images of endogenously tagged tubulin beta 4B with Hpep11. Scale bar: 10 μm (overview) and 2 μm (magnification). (f) Intensity profiles along tubulin filaments (red and blue lines in e) Means ± s.d. of the filament diameters were calculated as full width at half maximum (FWHM) from n=20 microtubule filaments, ≥ 2 images. A slight increase in cytosolic signal was noted in cells tagged with split-HaloTagat TUBB4B, compared to cells tagged with the full-length HaloTag, which may result from the presence of unbound but labeled cpHaloΔ3. All images were acquired after labeling with CA-SiR [100 nM] for one hour.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:uf4m7zlmcjlg2biwn4s2k32y/bafkreielty6fex37xy4caechbw5ebo3eypyiyvxxhkigeazd5gmy6tvlay@jpeg)
June 17, 2025 at 11:09 AM
New preprint by @kjohnsson.bsky.social lab!
A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing.
The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.
A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing.
The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.
FastAct_X: the 3rd actin probes generation from Spirochrome
✅ Improved actin ligand, captures very fast actin dynamics
✅ No undesired F-actin stabilization
✅ Just add & image protocol
✅ High brightness
✅ STED compatible
✅ Available in two colors SPY555 & SPY650
spirochrome.com/products/?_r...
✅ Improved actin ligand, captures very fast actin dynamics
✅ No undesired F-actin stabilization
✅ Just add & image protocol
✅ High brightness
✅ STED compatible
✅ Available in two colors SPY555 & SPY650
spirochrome.com/products/?_r...
March 13, 2025 at 11:59 AM
FastAct_X: the 3rd actin probes generation from Spirochrome
✅ Improved actin ligand, captures very fast actin dynamics
✅ No undesired F-actin stabilization
✅ Just add & image protocol
✅ High brightness
✅ STED compatible
✅ Available in two colors SPY555 & SPY650
spirochrome.com/products/?_r...
✅ Improved actin ligand, captures very fast actin dynamics
✅ No undesired F-actin stabilization
✅ Just add & image protocol
✅ High brightness
✅ STED compatible
✅ Available in two colors SPY555 & SPY650
spirochrome.com/products/?_r...
📢New product launch:
SPY555-FastAct_X:
✅ Improved actin ligand captures very fast actin dynamics
✅ No toxicity
✅ High brightness
✅ Low phototoxicity
✅ High photoblueing & bleaching resistance
spirochrome.com/product/spy5...
Image: @lindawedemann.bsky.social from @mschuhmacher.bsky.social lab
SPY555-FastAct_X:
✅ Improved actin ligand captures very fast actin dynamics
✅ No toxicity
✅ High brightness
✅ Low phototoxicity
✅ High photoblueing & bleaching resistance
spirochrome.com/product/spy5...
Image: @lindawedemann.bsky.social from @mschuhmacher.bsky.social lab
March 4, 2025 at 12:07 PM
📢New product launch:
SPY555-FastAct_X:
✅ Improved actin ligand captures very fast actin dynamics
✅ No toxicity
✅ High brightness
✅ Low phototoxicity
✅ High photoblueing & bleaching resistance
spirochrome.com/product/spy5...
Image: @lindawedemann.bsky.social from @mschuhmacher.bsky.social lab
SPY555-FastAct_X:
✅ Improved actin ligand captures very fast actin dynamics
✅ No toxicity
✅ High brightness
✅ Low phototoxicity
✅ High photoblueing & bleaching resistance
spirochrome.com/product/spy5...
Image: @lindawedemann.bsky.social from @mschuhmacher.bsky.social lab
Highly impressive LLSM imaging of PKmito Orange stained cells using DeepCristae, a newly published mitochondrial cristae restoration convolutional neural network.
PKMito Orange: spirochrome.com/product/pkmi...
Source Article: doi.org/10.1038/s420...
Happy #FluorescenceFriday
PKMito Orange: spirochrome.com/product/pkmi...
Source Article: doi.org/10.1038/s420...
Happy #FluorescenceFriday
February 28, 2025 at 9:54 AM
Highly impressive LLSM imaging of PKmito Orange stained cells using DeepCristae, a newly published mitochondrial cristae restoration convolutional neural network.
PKMito Orange: spirochrome.com/product/pkmi...
Source Article: doi.org/10.1038/s420...
Happy #FluorescenceFriday
PKMito Orange: spirochrome.com/product/pkmi...
Source Article: doi.org/10.1038/s420...
Happy #FluorescenceFriday
Which actin probe is best for your experiment?
Our new Actin_TestKit combines our 3 far red actin probes SiR-actin; SPY650-FastAct & SPY650-FastAct_X!
You can now screen for the best actin probe at a fraction of the cost of a single tube!
spirochrome.com/product/acti...
Our new Actin_TestKit combines our 3 far red actin probes SiR-actin; SPY650-FastAct & SPY650-FastAct_X!
You can now screen for the best actin probe at a fraction of the cost of a single tube!
spirochrome.com/product/acti...
February 6, 2025 at 1:10 PM
Which actin probe is best for your experiment?
Our new Actin_TestKit combines our 3 far red actin probes SiR-actin; SPY650-FastAct & SPY650-FastAct_X!
You can now screen for the best actin probe at a fraction of the cost of a single tube!
spirochrome.com/product/acti...
Our new Actin_TestKit combines our 3 far red actin probes SiR-actin; SPY650-FastAct & SPY650-FastAct_X!
You can now screen for the best actin probe at a fraction of the cost of a single tube!
spirochrome.com/product/acti...
📢New Actin Probe available at Spirochrome!
Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!
spirochrome.com/product/spy6...
The product is now available from our distributors
Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...
Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!
spirochrome.com/product/spy6...
The product is now available from our distributors
Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...
February 6, 2025 at 11:45 AM
📢New Actin Probe available at Spirochrome!
Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!
spirochrome.com/product/spy6...
The product is now available from our distributors
Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...
Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!
spirochrome.com/product/spy6...
The product is now available from our distributors
Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...