Spirochrome
@spirochrome.com
Our main focus is to offer innovative tools for live cell fluorescence imaging to the scientific community.
https://spirochrome.com/
https://spirochrome.com/
You are correct! mtDNA staining is typically performed with an intercalating DNA dye with nanomolar affinity for dsDNA. SPY-DNA probes, primarily designed for live cell imaging, are DNA groove binders and have a lower affinity to avoid toxicity. Probably not strong enough for robust mtDNA staining.
January 9, 2026 at 8:47 AM
You are correct! mtDNA staining is typically performed with an intercalating DNA dye with nanomolar affinity for dsDNA. SPY-DNA probes, primarily designed for live cell imaging, are DNA groove binders and have a lower affinity to avoid toxicity. Probably not strong enough for robust mtDNA staining.
The images were acquired on a @leicamicrosystems.bsky.social Stellaris system by
@bioarm.bsky.social from @centriolelab.bsky.social.
Huge thank you for trying this out!
@bioarm.bsky.social from @centriolelab.bsky.social.
Huge thank you for trying this out!
January 8, 2026 at 2:25 PM
The images were acquired on a @leicamicrosystems.bsky.social Stellaris system by
@bioarm.bsky.social from @centriolelab.bsky.social.
Huge thank you for trying this out!
@bioarm.bsky.social from @centriolelab.bsky.social.
Huge thank you for trying this out!
Are you staining DNA in your ExM samples?
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
January 8, 2026 at 2:25 PM
Are you staining DNA in your ExM samples?
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
Reposted by Spirochrome
Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well!
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
January 5, 2026 at 12:54 PM
Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well!
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well!
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
January 5, 2026 at 12:54 PM
Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well!
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
Peroxi_SPY can thus be combined with immunofluorescence labeling.
A new article provides a detailed protocol: app.jove.com/t/69405/pero...
Products link: spirochrome.com/products/?_c...
Reposted by Spirochrome
📄New article!
Fixed cell Peroxisome imaging is now possible with Peroxi_SPY probes
In a new JoVE article Triana Amen, @mschuhmacher.bsky.social and Einat Zalckvar robustly show that Peroxi_SPY stained cells can be fixed and stained by IF!
Detailed protocol in the article link (first reply below⬇️)
Fixed cell Peroxisome imaging is now possible with Peroxi_SPY probes
In a new JoVE article Triana Amen, @mschuhmacher.bsky.social and Einat Zalckvar robustly show that Peroxi_SPY stained cells can be fixed and stained by IF!
Detailed protocol in the article link (first reply below⬇️)
January 5, 2026 at 10:12 AM
📄New article!
Fixed cell Peroxisome imaging is now possible with Peroxi_SPY probes
In a new JoVE article Triana Amen, @mschuhmacher.bsky.social and Einat Zalckvar robustly show that Peroxi_SPY stained cells can be fixed and stained by IF!
Detailed protocol in the article link (first reply below⬇️)
Fixed cell Peroxisome imaging is now possible with Peroxi_SPY probes
In a new JoVE article Triana Amen, @mschuhmacher.bsky.social and Einat Zalckvar robustly show that Peroxi_SPY stained cells can be fixed and stained by IF!
Detailed protocol in the article link (first reply below⬇️)
A new article by @zhixingchen2.bsky.social shows that the far red mitochondrial probe PKmito Deep Red is a robust mitochondrial membrane potential probe when imaged by FLIM.
www.nature.com/articles/s41...
PKmito Deep Red is exclusively available at Spirochrome:
spirochrome.com/product/pkmi...
www.nature.com/articles/s41...
PKmito Deep Red is exclusively available at Spirochrome:
spirochrome.com/product/pkmi...
December 16, 2025 at 12:39 PM
A new article by @zhixingchen2.bsky.social shows that the far red mitochondrial probe PKmito Deep Red is a robust mitochondrial membrane potential probe when imaged by FLIM.
www.nature.com/articles/s41...
PKmito Deep Red is exclusively available at Spirochrome:
spirochrome.com/product/pkmi...
www.nature.com/articles/s41...
PKmito Deep Red is exclusively available at Spirochrome:
spirochrome.com/product/pkmi...
Reposted by Spirochrome
New paper from @zhixingchen2.bsky.social's lab!
It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!
www.nature.com/articles/s41...
It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!
www.nature.com/articles/s41...
December 16, 2025 at 12:11 PM
New paper from @zhixingchen2.bsky.social's lab!
It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!
www.nature.com/articles/s41...
It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime!
www.nature.com/articles/s41...
The unique mechanosensitive probe Flipper-TR is at the core of an amazing article by @juanmagararc.bsky.social and @rouxlab.bsky.social team of membrane tension experts.
Congratulations!
There is a great 🧵 explaining the key findings how and why cells build membrane tension gradients ⬇️
Congratulations!
There is a great 🧵 explaining the key findings how and why cells build membrane tension gradients ⬇️
New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen
November 28, 2025 at 7:59 AM
The unique mechanosensitive probe Flipper-TR is at the core of an amazing article by @juanmagararc.bsky.social and @rouxlab.bsky.social team of membrane tension experts.
Congratulations!
There is a great 🧵 explaining the key findings how and why cells build membrane tension gradients ⬇️
Congratulations!
There is a great 🧵 explaining the key findings how and why cells build membrane tension gradients ⬇️
Reposted by Spirochrome
New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen
November 27, 2025 at 2:14 PM
New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen
Reposted by Spirochrome
A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
November 24, 2025 at 1:34 PM
A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
The images in the main text are also definitely worth a look!
November 24, 2025 at 1:34 PM
The images in the main text are also definitely worth a look!
A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
November 24, 2025 at 1:34 PM
A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
Reposted by Spirochrome
Cool, the HAK-actin preprint was showcased by @prelights.bsky.social
A good reason to read it if you haven't yet 😜
A good reason to read it if you haven't yet 😜
Reliable actin labelling in expansion #microscopy 🔬
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton - preLights
HAK-actin is a new probe that may help researchers see the actin cytoskeleton in sharper detail using expansion microscopy.
prelights.biologists.com
November 19, 2025 at 12:28 PM
Cool, the HAK-actin preprint was showcased by @prelights.bsky.social
A good reason to read it if you haven't yet 😜
A good reason to read it if you haven't yet 😜
Reposted by Spirochrome
Quite a busy day on #Bluesky !
Been a while since we have been #preLight -ed !
Discover HAK-Actin if you haven't already ;)
Been a while since we have been #preLight -ed !
Discover HAK-Actin if you haven't already ;)
Reliable actin labelling in expansion #microscopy 🔬
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton - preLights
HAK-actin is a new probe that may help researchers see the actin cytoskeleton in sharper detail using expansion microscopy.
prelights.biologists.com
November 19, 2025 at 12:36 PM
Thank you @prelights.bsky.social for highlighting HAK-actin!
HAK-actin is a great tool for ExM actin staining.
spirochrome.com/product/hak-...
HAK-actin is a great tool for ExM actin staining.
spirochrome.com/product/hak-...
Reliable actin labelling in expansion #microscopy 🔬
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton - preLights
HAK-actin is a new probe that may help researchers see the actin cytoskeleton in sharper detail using expansion microscopy.
prelights.biologists.com
November 20, 2025 at 9:54 AM
Thank you @prelights.bsky.social for highlighting HAK-actin!
HAK-actin is a great tool for ExM actin staining.
spirochrome.com/product/hak-...
HAK-actin is a great tool for ExM actin staining.
spirochrome.com/product/hak-...
Reposted by Spirochrome
Klein Scientific telling us about the products they distribute including @spirochrome.com products. @globalbioimaging.bsky.social #GBI_AU_2025
November 18, 2025 at 4:09 AM
Klein Scientific telling us about the products they distribute including @spirochrome.com products. @globalbioimaging.bsky.social #GBI_AU_2025
More information about Flipper-TR on our website spirochrome.com/product/flip...
Flipper-TR® - Live cell membrane tension probe - spirochrome
Flipper-TR® is a live cell membrane tension probe
spirochrome.com
November 4, 2025 at 9:19 AM
More information about Flipper-TR on our website spirochrome.com/product/flip...
👉🦠Interested in bacterial membrane tension?
Our membrane tension probe Flipper-TR is a top tool for the job!
A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension.
🔗 doi.org/10.1073/pnas...
🧪
Our membrane tension probe Flipper-TR is a top tool for the job!
A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension.
🔗 doi.org/10.1073/pnas...
🧪
Exploring bacteria–surface interactions with a fluorescent membrane tension probe | PNAS
Understanding how bacteria interact with surfaces is critical for advancing applications
in biofilm and biofouling prevention, biomaterial developm...
doi.org
November 4, 2025 at 9:19 AM
👉🦠Interested in bacterial membrane tension?
Our membrane tension probe Flipper-TR is a top tool for the job!
A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension.
🔗 doi.org/10.1073/pnas...
🧪
Our membrane tension probe Flipper-TR is a top tool for the job!
A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension.
🔗 doi.org/10.1073/pnas...
🧪
Reposted by Spirochrome
October 29, 2025 at 9:30 AM
Reposted by Spirochrome
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
October 29, 2025 at 9:30 AM
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
And thanks to @joachimgoedhart.bsky.social for the Bouncy-Curtain imageJ plugin.
github.com/JoachimGoedh...
github.com/JoachimGoedh...
GitHub - JoachimGoedhart/Bouncy-Curtain: ImageJ/FIJI macro to generate an animated, curtain-style comparison of two images
ImageJ/FIJI macro to generate an animated, curtain-style comparison of two images - JoachimGoedhart/Bouncy-Curtain
github.com
October 29, 2025 at 9:44 AM
And thanks to @joachimgoedhart.bsky.social for the Bouncy-Curtain imageJ plugin.
github.com/JoachimGoedh...
github.com/JoachimGoedh...
October 29, 2025 at 9:30 AM
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
October 29, 2025 at 9:30 AM
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
October 7, 2025 at 8:21 AM
An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!