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Tanner Fadero

@tanner-fadero.bsky.social

360 followers 440 following 86 posts

(he/him) Light microscopy scientist interested in developing and deploying better live-cell fluorescence microscopy technology. Tweets about microscopy🔬, cell biology, and optics. Conflicts of interest: http://tinyurl.com/y25ctubo

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Tanner Fadero @tanner-fadero.bsky.social · 15d

Yes, there's a nice trick to compensating for transferring possession to the table from the crane. But there's also certain steps you can take before giving possession to the crane that decrease tension, leading to less severe shifts during future transfers. I'd love to walk you through them!

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Tanner Fadero @tanner-fadero.bsky.social · 20d

the anticipation is killing me

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Tanner Fadero @tanner-fadero.bsky.social · 20d

(I see nothing)

Tanner Fadero @tanner-fadero.bsky.social · 21d

@jsdaniel02.bsky.social can make it happen!

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Reposted by Tanner Fadero

Kari Herrington @kahsage.bsky.social · 21d

@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.

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Tanner Fadero @tanner-fadero.bsky.social · 28d

www.ascb.org/society-news...

ASCB and MBoC Announce 2025 Paper of the Year Award Winners - ASCB

ASCB and its research journal, Molecular Biology of the Cell (MBoC), are proud to recognize the outstanding scientific achievements of early career researchers through the 2025 Paper of the Year Award...

www.ascb.org

Reposted by Tanner Fadero

Alfred Millett-Sikking @amsikking.bsky.social · Sep 1

What if we could use 'any' immersion objective and get good 3D imaging? Normally we can't as we must refractive index match the immersion to the sample (previous post).

-> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!

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Tanner Fadero @tanner-fadero.bsky.social · Aug 30

But yeah, a modest tilt angle between projections looks similar!

Tanner Fadero @tanner-fadero.bsky.social · Aug 30

Oh neat! You're looking at a projection from the "native" angle. Interestingly, the closer your sheet tilt angle is to 0°, the more similar the native projection is to the normal Z projection.

Tanner Fadero @tanner-fadero.bsky.social · Aug 30

If that projection is from the deskew plug-in, then it's a true max intensity Z projection (where Z is O1's optical axis).

The deskew plug-in deskews AND rotates your data.

Reposted by Tanner Fadero

Torsten Wittmann @microtubule.bsky.social · Aug 28

Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.

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Reposted by Tanner Fadero

BWJones @bwjones.bsky.social · Aug 27

Bluesky is now the platform of choice for the scientific community.

From @jenlucpiquant.bsky.social on @arstechnica.com

arstechnica.com/science/2025...

Bluesky now platform of choice for science community

It’s not just you. Survey says: “Twitter sucks now and all the cool kids are moving to Bluesky”…

arstechnica.com

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Tanner Fadero @tanner-fadero.bsky.social · Aug 29

Chroma said no, but thorlabs seems interested!

Tanner Fadero @tanner-fadero.bsky.social · Aug 28

Optics folks: does anyone know a manufacturer of a dichroic beamsplitter *cube*?

Essentially, a dichroic version of this (for fluorescence microscopy):

Tanner Fadero @tanner-fadero.bsky.social · Aug 21

Science hive mind!

Does anyone do hardware tech dev on MRI or CT? Or do you know someone who does? I'd love an introduction, if so. Let me know!

Tanner Fadero @tanner-fadero.bsky.social · Aug 21

It's playing, you've just learned how to tune it out after so many builds

Tanner Fadero @tanner-fadero.bsky.social · Aug 21

Also, this build doesn't actually take six months. I was mostly waiting on parts!

Tanner Fadero @tanner-fadero.bsky.social · Aug 20

Here's a six-month time-lapse of me building the single objective light sheet fluorescence microscope at the UCSF Center for Advanced Light Microscopy!

Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social.

#snouty

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Tanner Fadero @tanner-fadero.bsky.social · Aug 20

You should get @jsdaniel02.bsky.social to help you add in a 808 laser!

Tanner Fadero @tanner-fadero.bsky.social · Aug 19

I noticed that too! StayGold seems to have a very short triplet lifetime, suggesting that it's already doing a similar triplet-quenching mechanism to what is happening with infrared. StayGold should definitely be a first target for directed evolution. Imagine StayGold with infrared depletion!

Tanner Fadero @tanner-fadero.bsky.social · Aug 19

Interesting! What's more interesting to me is not "what proteins does this work in?" and instead "can this be engineered with directed evolution?"

We got this effect in GFP by *accident*, and the primary sequence of the proteins seems to have huge influence over how strong this effect is.

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Tanner Fadero @tanner-fadero.bsky.social · Aug 19

In summary, for < $10,000 USD and less than a day's work, you can more than double the efficiency of your light sheet fluorescence microscope!

Tanner Fadero @tanner-fadero.bsky.social · Aug 19

Unlike confocal, light sheet also doesn't need to deplete triplets outside of the focal plane, because light sheet doesn't *generate* out-of-plane triplets.

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Tanner Fadero @tanner-fadero.bsky.social · Aug 19

That brings us to light sheet fluorescence microscopy. Unlike widefield, it's easy to achieve the infrared intensity needed to reduce photobleaching in light sheet because the infrared laser is focused into a line. Here, we're getting a 2.5-fold reduction using a 200-mW 808-nm laser from Vortran.

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