OK If we are moving to Bluesky I am rescuing my favourite ever twitter thread (Jan 2019).

The renamed:

Bluesky-sized history of neuroscience (biased by my interests)

The most used and often misapplied or overdone image adjustment for publication figures is contrast.

The 5D Contrast Optimizer prevents introduction of perceptually misleading artifacts and adjusts multidimensional fluorescence images while retaining 100% optical comparability in a few clicks!

I've just migrated all the followers on the old place who are on Blusky using this tool www.wikihow.com/Import-Twitt... Ignore the code in the picture - you have to install one app and input your bluesky details. Worked so well, I even gave a donation to its creator.

As PIs we can mentor our team to select colorblind friendly pseudo colors at acquisition time 🔬and share free (and super cool) resources like colororacle.org and medialab.github.io/iwanthue/ that helps you select or design color friendly palettes for projects that have many categories 💻 #dataViz

A PSA for PIs: If you have a light microscope, please have watch your lab watch these short practical videos on how to prevent damage to your objective lenses! Trust me on this one, they need it...
#savetheobjectives

youtube.com/playlist?lis...

As a result, GECIs have an impressive contrast and large fold-changes in intensity. This enables in vivo imaging in complex tissue. ⬇️ This is one of my favourite examples - imaging neuronal activity in a Zebrafish brain
Source: doi.org/10.1038/nmet...