Joachim Goedhart
@joachimgoedhart.bsky.social
Scientist + Teacher UvA/Amsterdam | Cells | Molecules | Microscopy | Fluorescent Proteins | Biosensors | Lifetime imaging | Open Science | dataViz | R | web apps
Homepage: https://joachimgoedhart.github.io/
DataViz Apps: https://huygens.science.uva.nl
Homepage: https://joachimgoedhart.github.io/
DataViz Apps: https://huygens.science.uva.nl
Pinned
There are now 24 step-by-step guides, explaining how to create these ⬇️ data visualizations with R/ggplot2 - I hope this is a useful resource. Find them all here: joachimgoedhart.github.io/DataViz-prot...
Reposted by Joachim Goedhart
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
November 25, 2025 at 8:41 PM
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
Reposted by Joachim Goedhart
For some research studies the optimal sample size should be estimated at 0
November 25, 2025 at 10:51 AM
For some research studies the optimal sample size should be estimated at 0
Redesign of Calmodulin for Genetically Encoded Zn2+ Sensing with High Dynamic Range
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
Redesign of Calmodulin for Genetically Encoded Zn2+ Sensing with High Dynamic Range
The development of genetically encoded Zn2+ probes (GEZPs) has been hindered by the limited signal transduction efficiency of natural zinc-binding domains, but the encoded Ca2+ indicators utilize the ...
pubs.acs.org
November 25, 2025 at 8:35 AM
Redesign of Calmodulin for Genetically Encoded Zn2+ Sensing with High Dynamic Range
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
In our new #Preprint, we pilot a revolutionary new idea: no supplemental data (it has 21 figures, though):
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
www.biorxiv.org/content/10.1...
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
www.biorxiv.org/content/10.1...
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
We have previously shown that the calcium sensing receptor (CaSR) activates different G proteins and second messengers in single cells, and that GPCR and G protein-mediated ERK activity can be highly ...
www.biorxiv.org
November 24, 2025 at 8:29 AM
In our new #Preprint, we pilot a revolutionary new idea: no supplemental data (it has 21 figures, though):
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
www.biorxiv.org/content/10.1...
Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR
www.biorxiv.org/content/10.1...
Reposted by Joachim Goedhart
{ripper} dissects your #Quarto documents, extracting code blocks by language and leaving executable scripts behind.
Supports #rstats, #python, #julia, and 13 other victims.
For those who never stopped celebrating Halloween.
📚 quarto.thecoatlessprofessor.com/ripper/
💻 github.com/coatless-qua...
Supports #rstats, #python, #julia, and 13 other victims.
For those who never stopped celebrating Halloween.
📚 quarto.thecoatlessprofessor.com/ripper/
💻 github.com/coatless-qua...
November 22, 2025 at 10:57 PM
{ripper} dissects your #Quarto documents, extracting code blocks by language and leaving executable scripts behind.
Supports #rstats, #python, #julia, and 13 other victims.
For those who never stopped celebrating Halloween.
📚 quarto.thecoatlessprofessor.com/ripper/
💻 github.com/coatless-qua...
Supports #rstats, #python, #julia, and 13 other victims.
For those who never stopped celebrating Halloween.
📚 quarto.thecoatlessprofessor.com/ripper/
💻 github.com/coatless-qua...
Revealing acute consequences of rapid degradation of synaptic fusion proteins at individual synapses using Auxin-Inducible Degron 2 technology
www.nature.com/articles/s42...
www.nature.com/articles/s42...
November 22, 2025 at 7:59 PM
Revealing acute consequences of rapid degradation of synaptic fusion proteins at individual synapses using Auxin-Inducible Degron 2 technology
www.nature.com/articles/s42...
www.nature.com/articles/s42...
Reposted by Joachim Goedhart
Life actually: over taaie systemen en bonte details.
Mijn oratie van 20 november 2025 komt binnenkort nog beschikbaar als PDF maar is nu al terug te kijken via tinyurl.com/OratieRenee
Mijn oratie van 20 november 2025 komt binnenkort nog beschikbaar als PDF maar is nu al terug te kijken via tinyurl.com/OratieRenee
November 21, 2025 at 7:55 PM
Life actually: over taaie systemen en bonte details.
Mijn oratie van 20 november 2025 komt binnenkort nog beschikbaar als PDF maar is nu al terug te kijken via tinyurl.com/OratieRenee
Mijn oratie van 20 november 2025 komt binnenkort nog beschikbaar als PDF maar is nu al terug te kijken via tinyurl.com/OratieRenee
Reposted by Joachim Goedhart
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
November 20, 2025 at 11:42 AM
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
Reposted by Joachim Goedhart
Soliciting recommendations for 3D printers and lab supply blue prints. Please share and RT.
November 21, 2025 at 2:24 AM
Soliciting recommendations for 3D printers and lab supply blue prints. Please share and RT.
Reposted by Joachim Goedhart
quick test with SAM3 - impressed with how well it seems to track long protrusions? prompted for "cell", does not find anything when looking for "fibroblast" or "nucleus"
November 19, 2025 at 8:15 PM
quick test with SAM3 - impressed with how well it seems to track long protrusions? prompted for "cell", does not find anything when looking for "fibroblast" or "nucleus"
Reposted by Joachim Goedhart
Check out this tick I collected ;-)
And by collected, I mean pulled off my pant leg after a walk in the woods
And by collected, I mean pulled off my pant leg after a walk in the woods
November 19, 2025 at 8:29 PM
Check out this tick I collected ;-)
And by collected, I mean pulled off my pant leg after a walk in the woods
And by collected, I mean pulled off my pant leg after a walk in the woods
Uploading >30 files to change our preprint into a VOR for @elife.bsky.social
What could possibly go wrong?
What could possibly go wrong?
November 19, 2025 at 1:58 PM
Uploading >30 files to change our preprint into a VOR for @elife.bsky.social
What could possibly go wrong?
What could possibly go wrong?
The annual course “Basics in Fluorescence Microscopy”, will be organised by the van Leeuwenhoek Centre for Advanced Microscopy from January 27th - 30th, 2026.
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Basics in fluorescence microscopy – Microscopycourse
www.microscopycourse.nl
November 19, 2025 at 1:48 PM
The annual course “Basics in Fluorescence Microscopy”, will be organised by the van Leeuwenhoek Centre for Advanced Microscopy from January 27th - 30th, 2026.
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Location: University of Amsterdam (NL)
For more info, preliminary program and the application form check www.microscopycourse.nl?page_id=78
Reposted by Joachim Goedhart
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
November 19, 2025 at 6:27 AM
Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
Reposted by Joachim Goedhart
Major new direction in the lab: hacking human cell biology with pathogen effectors (eORFs) - amazing collaboration with @miketilapia.bsky.social lab. Huge congrats to first authors Tomas & He & all co-authors! Check out the pre-print on @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...
www.biorxiv.org
November 18, 2025 at 2:51 PM
Major new direction in the lab: hacking human cell biology with pathogen effectors (eORFs) - amazing collaboration with @miketilapia.bsky.social lab. Huge congrats to first authors Tomas & He & all co-authors! Check out the pre-print on @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...
Reposted by Joachim Goedhart
Reposted by Joachim Goedhart
Reposted by Joachim Goedhart
🔗 to paper: www.sciencedirect.com/science/arti...
When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.
November 14, 2025 at 8:42 PM
🔗 to paper: www.sciencedirect.com/science/arti...
Reposted by Joachim Goedhart
When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.
November 14, 2025 at 8:34 PM
When a larval zebrafish twitches its tail, the vascular endothelium lights up with a calcium signal, mediated by Piezo1. We used to think mechanosensing in endothelial cells was about sensing blood flow, but at least in this case it's about sensing body motion.
Reposted by Joachim Goedhart
Broader #bioimaging and #microsky people - anyone have any suggestions for sources of ~0.25mm non-cytotoxic double sided adhesive sheets?
Alternatively, a DIY solution might be possible. If anyone has any ideas on a suitable non-toxic double-sided adhesive sheet material, then purchasing those and using either consumer grade die-cutter or online-die cutting services could be a straightforward solution.
November 14, 2025 at 10:44 AM
Broader #bioimaging and #microsky people - anyone have any suggestions for sources of ~0.25mm non-cytotoxic double sided adhesive sheets?
Reposted by Joachim Goedhart
People in the audience looking at the y-axis labels
November 14, 2025 at 7:21 AM
People in the audience looking at the y-axis labels
Reposted by Joachim Goedhart
We didn't want to pay $800 for a TLC plate cutter, so we built one of our own – here's how you can too.
An Open-Source, Low-Cost Thin-Layer Chromatography Plate Cutter
Thin-layer chromatography (TLC) is widely used in teaching and research laboratories, but TLC plate cutters remain expensive, with most commercial models priced above $800 USD. This article presents a...
pubs.acs.org
November 13, 2025 at 8:18 PM
We didn't want to pay $800 for a TLC plate cutter, so we built one of our own – here's how you can too.
Reposted by Joachim Goedhart
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 4:20 PM
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
How genetically encoded sensors have lit up neuroscience
www.nature.com/articles/d41...
www.nature.com/articles/d41...
How genetically encoded sensors have lit up neuroscience
Tools that track specific molecules in neurons have enabled researchers to probe previously unexplored aspects of neurobiology — although important caveats remain.
www.nature.com
November 13, 2025 at 3:17 PM
How genetically encoded sensors have lit up neuroscience
www.nature.com/articles/d41...
www.nature.com/articles/d41...
Reposted by Joachim Goedhart
✨ New preprint ✨ What if we could watch AND control thousands of individual neurons in behaving mice—with stable access over months? 🧠⚡ Our work introduces transgenic all-optical tools that make this possible - and a resulting surprising discovery! 🔭💡
www.biorxiv.org/content/10.1... 1/13 🧵
www.biorxiv.org/content/10.1... 1/13 🧵
Large-scale cellular-resolution read/write of activity enables discovery of cell types defined by complex circuit properties
The complexity of the mammalian brain’s vast population of interconnected neurons poses a formidable challenge to elucidate its underlying mechanisms of coordination and computation. A key step forwar...
www.biorxiv.org
November 13, 2025 at 12:24 AM
✨ New preprint ✨ What if we could watch AND control thousands of individual neurons in behaving mice—with stable access over months? 🧠⚡ Our work introduces transgenic all-optical tools that make this possible - and a resulting surprising discovery! 🔭💡
www.biorxiv.org/content/10.1... 1/13 🧵
www.biorxiv.org/content/10.1... 1/13 🧵