Lightnews — Scholar-powered news

Reposted by Séamus Holden 🔬🦠🧫

ᐯIᑕTOᖇ ᑎIᘔET, ᗰᗪ @nizet.bsky.social · 3d

C̳H̳E̳C̳K̳ ̳I̳T̳ ̳O̳U̳T̳

Spectacular atomic force microscopy time course studies from Carolina Borrelli et al. in Nature Microbiology show how polymyxin drugs (like colistin) cause blebbing and shedding of the Gram-negative bacterial outer membrane

It looks almost like the bacilli 🦠 caught smallpox then died

Polymyxin B lethality requires energy-dependent outer membrane disruption - Nature Microbiology

The antibiotic polymyxin B requires bacterial metabolic activity to cause sufficient damage to the outer membrane to access the inner membrane, which it permeabilizes via an energy-independent mechanism to kill the cell.

www.nature.com

4 17

Reposted by Séamus Holden 🔬🦠🧫

David Legland @dlegland.bsky.social · 10d

Thrilled to share that our article in @jofmicroscopy.bsky.social is now accessible online and as open access! If you wonder how to start the analysis of your images, we propose a decision tree to help identify suitable methods. Just follow the link(s)...

#imageanalysis #bioimaging

jofmicroscopy.bsky.social @jofmicroscopy.bsky.social · 11d

"From cells to pixels: A decision tree for designing bioimage analysis pipelines" by Elnaz Fazeli, Robert Haase, Michael Doube, Kota Miura & David Legland is free-to-view here: doi.org/10.1111/jmi....

7 10

Reposted by Séamus Holden 🔬🦠🧫

Freya Harrison @friendlymicrobe.bsky.social · 5d

#MicroSky

Paul Hoskisson 🧫 🦠🐸 @paulhoskisson.bsky.social · 10d

Come and work with me and @ariannebabina.bsky.social on #Streptomyces evolution and antibiotic production

Origins of a tangled bank: Adaptation and evolution in antibiotic-producing Streptomyces

www.gla.ac.uk/postgraduate...

please repost

University of Glasgow - Postgraduate study - Centres for Doctoral Training - NorthWest Biosciences - Our Projects - Underpinning Bioscience - Paul A Hoskisson

www.gla.ac.uk

2 4

Reposted by Séamus Holden 🔬🦠🧫

AnniZLab: [🦠, 🧬 , ✨] @annizlab.bsky.social · 5d

BindCraft: an AlphaFold2-powered de novo protein binder design tool 🧬✨
Tested in some bacterial systems too! Designs so far are short peptides or mini-domains. Looking forward to long/full protein design ahead! 🧪 #microsky
www.nature.com/articles/s41...

One-shot design of functional protein binders with BindCraft - Nature

BindCraft, an open-source, automated pipeline for de novo protein binder design with experimental success rates of 10–100%, leverages AlphaFold2 weights to generate binders with nanomolar affinity without the need for high-throughput screening.

www.nature.com

10 41

Reposted by Séamus Holden 🔬🦠🧫

Zeynep Baharoglu @zeynepbaharoglu.bsky.social · 4d

And the crispri genetic interaction screen 👏💫🤯 #microsky from J-W Veening lab. Dual CRISPRi-seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle: Cell Systems www.cell.com/cell-systems...

Dual CRISPRi-seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle

Dénéréaz et al. introduce dual CRISPRi-seq, a broadly applicable method to chart gene-gene interactions across whole genomes. Applying it to the pathogen Streptococcus pneumoniae, they uncovered over ...

www.cell.com

2 5

Reposted by Séamus Holden 🔬🦠🧫

Emmanuele Severi @emmseveri.bsky.social · 3d

www.biorxiv.org/content/10.1...

#microsky

A load-bearing function for the cytoplasmic membrane of Escherichia coli

The structural integrity of bacterial cells is traditionally attributed to the peptidoglycan cell wall, and more recently to the outer membrane, with the cytoplasmic membrane assumed to be mechanicall...

www.biorxiv.org

5 9

Reposted by Séamus Holden 🔬🦠🧫

Sarah Svensson @campy-bara.bsky.social · 14d

Dual transposon sequencing profiles the genetic interaction landscape in bacteria | Science www.science.org/doi/10.1126/...

Dual transposon sequencing profiles the genetic interaction landscape in bacteria

Gene redundancy complicates systematic characterization of gene function as single-gene deletions may not produce discernible phenotypes. We report dual transposon sequencing (dual Tn-seq), a platform...

www.science.org

23 58

Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · 29d

Really enjoying reading this primer on single particle tracking #microscopy by @svanteeffelen.bsky.social and colleagues www.nature.com/articles/s43... (pdf on Sven's website)

A guide to single-particle tracking - Nature Reviews Methods Primers

Single-particle tracking is a method for analysing different motion behaviours of individual proteins and protein complexes in live cells. This Primer outlines the experimental setup and labelling tec...

www.nature.com

2 14 35

Reposted by Séamus Holden 🔬🦠🧫

christoph_STCmicrobeblog @christophstc.bsky.social · Sep 2

#MicroSky yeah, cable bacteria 🤗

Cameron Thrash @jcamthrash.bsky.social · Sep 2

End-to-end contact enables long-distance electron transport between filaments in cable bacteria academic.oup.com/ismej/advanc... #jcampubs

2 4

Reposted by Séamus Holden 🔬🦠🧫

Martin Pacesa @martinpacesa.bsky.social · Aug 27

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.

www.nature.com/articles/s41...

14 110 300

Reposted by Séamus Holden 🔬🦠🧫

V @vmc54.bsky.social · Jul 22

The sun cast incredible crespuscular rays during last night's saffron hued sunset.

Bay Ridge, Brooklyn

170 620 6.9K

Reposted by Séamus Holden 🔬🦠🧫

STCmicrobeblog @stcmicrobeblog.bsky.social · Jul 23

you know the chaperones that 𝘌. 𝘤𝘰𝘭𝘪 has at its disposal, don' you? GroEL/GroES, DnaK/DnaJ/GrpE, Trigger Factor (TF), ClpB, Hsp33 and Spy... but do you have the ribosome on your checklist? you should! 👇

www.biorxiv.org/content/10.1...
#CryoEM #CryoET #TeamTomo #MicroSky

Fig. 6. Cotranslational folding and assembly of β-gal. β-gal folds efficiently when coupled to ongoing translation, directed by the ribosome to synchronise with tetramer assembly. Domain 3 folds in discrete steps, the timing of which are dictated by an interaction with a hydrophobic groove on uL23 that stabilises a nascent amphipathic helix. The pioneering round of translation generates a full-length
subunit, which then binds via the long interface to a ribosome-associated subunit during synthesis of domain 5. An immature dimer results, which matures post-translation and assembles via the activating interface to form the folded tetramer. When uncoupled from translation, monomers fold via a less efficient pathway and are prone to misfold and misassemble.

8 23

Reposted by Séamus Holden 🔬🦠🧫

Emma Denham @gingermicrobe.bsky.social · Jul 23

Come and work with us in beautiful Bath.

tenuretracker.info @tenuretracker.bsky.social · Jul 1

University of Bath is hiring:
Lecturer in Biotechnology (Teaching)

3 2

Reposted by Séamus Holden 🔬🦠🧫

Hannah Brown 🧂🦠🔬 @hannjbrown.bsky.social · Nov 23

🚨 New paper alert! 🚨

MinD is famously known for positioning FtsZ at mid-cell in bacteria

In our study, myself and @iduggin.bsky.social found that MinD homologues in Haloferax volcanii influence the localisation of CetZ1, a homologue of FtsZ and tubulin

#Archaeasky #Microsky

Frontiers | MinD proteins regulate CetZ1 localization in Haloferax volcanii

url.au.m.mimecastprotect.com

1 14 44

Reposted by Séamus Holden 🔬🦠🧫

c0nc0rdance @c0nc0rdance.bsky.social · Jul 16

*15%* of all the biomass on Earth is buried deep in the crust.

70-90% of all bacterial (and archaeal) biomass is found at depths from a few meters to 10 km below the soil line.

We can call these deep denizens: 'INTRATERRESTRIALS'.

Let's talk about the Deep Biosphere.

(🧑‍🎨: Verena Heuer, Marum)

A diagram cross-section of the Earth's crust under the ocean and land, showing surface biospheres cycling carbon, deep geological processes above 120 C, and the deep biosphere making methane which enters the ocean via cold seeps.

CREDIT: Verena Heuer, for Marum (Center for Marine Environmental Sciences at Universitat Bremen)

5 23 100

Reposted by Séamus Holden 🔬🦠🧫

Morgan Carter @morgancarterphd.bsky.social · Jul 17

Going to a conference and hope someone posts about your talk? Make it easy for the posters!!

Tips:
1. Include your handle at beginning and end
2. Make clear what is published/not
3. Have a conclusions slide with key message
4. Highlight new preprint or publication if relevant

#AcademicSky 🦠🧪🧬

2 21 65

Reposted by Séamus Holden 🔬🦠🧫

Alejandro Montenegro @aemonten.bsky.social · Jul 16

They exist! Please tell the authors that when you review such papers 😊

Mike Feigin 🥯 @mikefeigin.bsky.social · Jul 16

I just reviewed a really good paper. Beautifully written, conclusions not overblown. Just solid, rigorous science. Thanks.

1 5 32

Reposted by Séamus Holden 🔬🦠🧫

Prof Tanya Parish @proftanya13.bsky.social · Jul 15

this book everythingistb.com 🦠🧪 #Tuberculosis

2 3 7

Reposted by Séamus Holden 🔬🦠🧫

John Innes Centre @johninnescentre.bsky.social · Jul 15

VACANCY - We’re seeking an Optical Microscopy Support Specialist to join our Bioimaging Platform, to help train users on light microscopes, collaborate on imaging projects and provide technical support.

www.jic.ac.uk/vacancies/op...

Closing date - 11 August 2025
Contract - Full time, indefinite

Optical Microscopy Support Specialist | John Innes Centre

An exciting opportunity has arisen for an Optical Microscopy Support Specialist to join the Bioimaging Platform at the John Innes Centre.

www.jic.ac.uk

18 12

Reposted by Séamus Holden 🔬🦠🧫

EMBRC (European Marine Biological Resource Centre) @embrc-eu.bsky.social · Jul 16

#EUProject | #BioImaging & AI

➡️ BioImage Model Zoo by @ai4life.bsky.social is a platform for #AI models in biological imaging

➡️ To find out about the training courses and tools available to help you use the platform correctly for your area of study, visit: www.embrc.eu/project/ai4life/

🌊🧪🦑

6 17

Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · Jul 7

Very interesting paper on structural biology and biochemistry of Staph aureus FtsW:PBP1 (PBP1 is the divisome bPBP partner). Amongst other things, looks like some very interesting results on interaction of PBP1 PASTA domains with peptidoglycan pmc.ncbi.nlm.nih.gov/articles/PMC... #MicroSky

Integrative structural biology of the penicillin-binding protein-1 from Staphylococcus aureus, an essential component of the divisome machinery

Keywords: Divisome, Staphylococcus aureus, X-ray crystal structure, SAXS in-solution structure, Molecular dynamics simulations, PBP1, PASTA domains, Antibiotics inhibition, Peptidoglycan binding

pmc.ncbi.nlm.nih.gov

2 5

Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · Jul 4

Nature Microbiology put together a good research briefing on this Rudner lab bacterial turgor regulation/ c-di-AMP paper.
A supportive quote from my peer review comments is included.
#microsky #bioimaging
rdcu.be/euQln

1 1 11

Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · Jun 26

I checked in with GraceBio. They are in process of moving eneFrame-like adhesive square frames from prototype to manufacturing. Once this is done they will be able to give us pricing and product codes.

4

Reposted by Séamus Holden 🔬🦠🧫

Guillaume Jacquemet @guijacquemet.bsky.social · Jun 23

Are you looking for a postdoc?
I will be looking for 2-4 people after the summer to join the lab !!
Do not hesitate to reach out and disseminate !!

87 67

Séamus Holden 🔬🦠🧫 @seamusholden.bsky.social · Jun 23

Really cool paper on turgor regulation in Bacillus subtilis www.nature.com/articles/s41...

Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis - Nature Microbiology

Brogan et al. uncover a signalling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases turgor ...

www.nature.com

9