Emmanuele Severi
@emmseveri.bsky.social
Research Fellow at Northumbria University: phage, synthetic biology, sialic acid, transport proteins. He/him.
Pinned
VOM-UK Conferences - NU-OMICS
Viruses of Microbes UK (VoM-UK) Conference The Virus of Microbes UK (VoM-UK) Conference is hosted by Microbiome Excellence Peak of Excellence at Northumbria University. This two-day conference takes place annually at the end of January/beginning of February and is a chance for researchers to gather and share their knowledge. The VoM-UK conference is designed to […]
hosting.northumbria.ac.uk
hosting.northumbria.ac.uk/nu-omics/vom...
#phagesky #phage #microsky
Come and join us for Viruses of Microbes UK 2026 at @northumbriauni.bsky.social ! Fantastic chance for ECRs to showcase their work 🙂
#phagesky #phage #microsky
Come and join us for Viruses of Microbes UK 2026 at @northumbriauni.bsky.social ! Fantastic chance for ECRs to showcase their work 🙂
#microsky #synbio
Optimised cumate-inducible system for Burkholderia cenocepacia.
www.biorxiv.org/content/10.1...
Optimised cumate-inducible system for Burkholderia cenocepacia.
www.biorxiv.org/content/10.1...
Generation of an optimised Cumate toolkit for tuneable protein expression during in vitro and in vivo studies of Burkholderia cenocepacia
Inducible gene expression is pivotal for dissecting bacterial physiology and virulence mechanisms. Across the Burkholderia genera, a limited range of inducible systems currently exist that show minima...
www.biorxiv.org
November 27, 2025 at 4:25 PM
#microsky #synbio
Optimised cumate-inducible system for Burkholderia cenocepacia.
www.biorxiv.org/content/10.1...
Optimised cumate-inducible system for Burkholderia cenocepacia.
www.biorxiv.org/content/10.1...
#microsky
Catabolite repression of H2 consumption in Msmeg. From @greening.bsky.social &Co.
www.biorxiv.org/content/10.1...
Catabolite repression of H2 consumption in Msmeg. From @greening.bsky.social &Co.
www.biorxiv.org/content/10.1...
Atmospheric hydrogen consumption is regulated by catabolite repression in mycobacteria
Consumption of atmospheric hydrogen (H2) enables diverse aerobic microorganisms to grow and persist in resource-deprived environments. In the aerobic saprophyte Mycobacterium smegmatis, hydrogen oxida...
www.biorxiv.org
November 27, 2025 at 4:17 PM
#microsky
Catabolite repression of H2 consumption in Msmeg. From @greening.bsky.social &Co.
www.biorxiv.org/content/10.1...
Catabolite repression of H2 consumption in Msmeg. From @greening.bsky.social &Co.
www.biorxiv.org/content/10.1...
Reposted by Emmanuele Severi
The host range of generalist and specialist phages in capsule-diverse Klebsiella hosts is driven by the evolvability of receptor-binding proteins
@plosbiology.org from @pilardomingoc.bsky.social
journals.plos.org/plosbiology/...
@plosbiology.org from @pilardomingoc.bsky.social
journals.plos.org/plosbiology/...
The host range of generalist and specialist phages in capsule-diverse Klebsiella hosts is driven by the evolvability of receptor-binding proteins
Capsule diversity limits phage host range by affecting RBP interactions in capsulated bacteria. This study shows that generalist phages evolve host range through RBP mutations and recombination, while...
journals.plos.org
November 27, 2025 at 12:28 PM
The host range of generalist and specialist phages in capsule-diverse Klebsiella hosts is driven by the evolvability of receptor-binding proteins
@plosbiology.org from @pilardomingoc.bsky.social
journals.plos.org/plosbiology/...
@plosbiology.org from @pilardomingoc.bsky.social
journals.plos.org/plosbiology/...
Reposted by Emmanuele Severi
Exciting new preprint from @amibhatt.bsky.social group
'A conserved sRNA regulates mucin adhesion and gut colonization across the Enterococcaceae'
www.biorxiv.org/content/10.1...
'A conserved sRNA regulates mucin adhesion and gut colonization across the Enterococcaceae'
www.biorxiv.org/content/10.1...
A conserved sRNA regulates mucin adhesion and gut colonization across the Enterococcaceae
Enterococci, particularly E. faecalis , can survive in diverse settings within and outside human hosts. The capacity of E. faecalis to colonize these locations relies on its ability to adapt by alteri...
www.biorxiv.org
November 26, 2025 at 2:44 PM
Exciting new preprint from @amibhatt.bsky.social group
'A conserved sRNA regulates mucin adhesion and gut colonization across the Enterococcaceae'
www.biorxiv.org/content/10.1...
'A conserved sRNA regulates mucin adhesion and gut colonization across the Enterococcaceae'
www.biorxiv.org/content/10.1...
Reposted by Emmanuele Severi
Very happy to have been part of this work from the Mariano lab, identifying 11 hotspots enriched in phage defence systems in Pseudomonas aeruginosa. It was great to get the chance to collaborate with @giusym1990.bsky.social again!
academic.oup.com/nargab/artic...
academic.oup.com/nargab/artic...
Validate User
academic.oup.com
November 25, 2025 at 11:00 PM
Very happy to have been part of this work from the Mariano lab, identifying 11 hotspots enriched in phage defence systems in Pseudomonas aeruginosa. It was great to get the chance to collaborate with @giusym1990.bsky.social again!
academic.oup.com/nargab/artic...
academic.oup.com/nargab/artic...
Reposted by Emmanuele Severi
Pleased to share our recent article in PNAS - a collaboration with @jessicaandreani.bsky.social & Pablo Radicella, with important roles played by many members of each team.
A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes www.pnas.org/doi/10.1073/...
A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes www.pnas.org/doi/10.1073/...
A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes | PNAS
Natural genetic transformation is a conserved mechanism of bacterial horizontal gene
transfer, which is directed entirely by the recipient cell and...
www.pnas.org
November 26, 2025 at 7:44 AM
Pleased to share our recent article in PNAS - a collaboration with @jessicaandreani.bsky.social & Pablo Radicella, with important roles played by many members of each team.
A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes www.pnas.org/doi/10.1073/...
A tripartite protein complex promotes DNA transport during natural transformation in Firmicutes www.pnas.org/doi/10.1073/...
Reposted by Emmanuele Severi
2 days left to apply for a fully funded MIBTP Phd project with me at the School of Life Sciences @warwicklifesci.bsky.social at Warwick. We will do viral functional metagenomics and other cool techniques.
Check out the details and apply in the link below
warwick.ac.uk/fac/cross_fa...
Check out the details and apply in the link below
warwick.ac.uk/fac/cross_fa...
November 25, 2025 at 2:54 PM
2 days left to apply for a fully funded MIBTP Phd project with me at the School of Life Sciences @warwicklifesci.bsky.social at Warwick. We will do viral functional metagenomics and other cool techniques.
Check out the details and apply in the link below
warwick.ac.uk/fac/cross_fa...
Check out the details and apply in the link below
warwick.ac.uk/fac/cross_fa...
#microsky #phagesky
Transposon mutagenesis of jumbo #phage using an anti-CRISPR factor as selectable marker
www.biorxiv.org/content/10.1...
Transposon mutagenesis of jumbo #phage using an anti-CRISPR factor as selectable marker
www.biorxiv.org/content/10.1...
Bacteriophage genome-wide transposon mutagenesis
Bacteriophages have genomes that span a wide size range, are densely packed with coding sequences, and frequently encode genes of unknown function. Classical forward genetics has defined essential gen...
www.biorxiv.org
November 25, 2025 at 8:07 AM
#microsky #phagesky
Transposon mutagenesis of jumbo #phage using an anti-CRISPR factor as selectable marker
www.biorxiv.org/content/10.1...
Transposon mutagenesis of jumbo #phage using an anti-CRISPR factor as selectable marker
www.biorxiv.org/content/10.1...
#microsky #phagesky
More on how Arbitrium regulates temperate #phage lifestyle in Bacillus
www.biorxiv.org/content/10.1...
More on how Arbitrium regulates temperate #phage lifestyle in Bacillus
www.biorxiv.org/content/10.1...
Arbitrium systems control lysis/lysogeny through the regulation of small antirepressor proteins
Many temperate Bacillus phages utilize the arbitrium signaling system to control lysis/lysogeny decisions. While the function of the arbitrium signal AimP and its receptor AimR are well known, it is u...
www.biorxiv.org
November 25, 2025 at 8:05 AM
#microsky #phagesky
More on how Arbitrium regulates temperate #phage lifestyle in Bacillus
www.biorxiv.org/content/10.1...
More on how Arbitrium regulates temperate #phage lifestyle in Bacillus
www.biorxiv.org/content/10.1...
Reposted by Emmanuele Severi
Now properly published at @natcomms.nature.com with few additional experiments incl. demonstration of Daptomycin's ability to depolarise non-growing cells.
See the original preprint-thread for a summary of our findings.
www.nature.com/articles/s41...
#microsky
See the original preprint-thread for a summary of our findings.
www.nature.com/articles/s41...
#microsky
November 24, 2025 at 8:20 PM
Now properly published at @natcomms.nature.com with few additional experiments incl. demonstration of Daptomycin's ability to depolarise non-growing cells.
See the original preprint-thread for a summary of our findings.
www.nature.com/articles/s41...
#microsky
See the original preprint-thread for a summary of our findings.
www.nature.com/articles/s41...
#microsky
Reposted by Emmanuele Severi
Very happy to share our work on lipopolysaccharide assembly by the Lpt complex, published today in @natcomms.nature.com
Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social
Congrats to all authors, especially Haoxiang, Axel & Violette
🔗 doi.org/10.1038/s414...
Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social
Congrats to all authors, especially Haoxiang, Axel & Violette
🔗 doi.org/10.1038/s414...
Structural basis of lipopolysaccharide assembly by the outer membrane translocon holo-complex - Nature Communications
The asymmetric distribution of lipopolysaccharide (LPS) on the surface of the bacterial outer membrane is essential and crucial for antibiotic resistance. Here, authors characterize the LPS translocon...
doi.org
November 24, 2025 at 3:22 PM
Very happy to share our work on lipopolysaccharide assembly by the Lpt complex, published today in @natcomms.nature.com
Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social
Congrats to all authors, especially Haoxiang, Axel & Violette
🔗 doi.org/10.1038/s414...
Fantastic collaboration with @raffaeleieva.bsky.social l and t @pstansfeld.bsky.social
Congrats to all authors, especially Haoxiang, Axel & Violette
🔗 doi.org/10.1038/s414...
Reposted by Emmanuele Severi
Synthetic bacterial community colonizes wheat roots grown in soil and mimics the assembly pattern of a field community
bioRxiv from @fredebak.bsky.social et al #NNF_INTERACT
www.biorxiv.org/content/10.1...
bioRxiv from @fredebak.bsky.social et al #NNF_INTERACT
www.biorxiv.org/content/10.1...
Synthetic bacterial community colonizes wheat roots grown in soil and mimics the assembly pattern of a field community
The root microbiome is important for plant development. The impact of the root microbiome is the sum of multiple complex interactions among microorganisms, the plant and the environment. This complexi...
www.biorxiv.org
November 24, 2025 at 7:43 AM
Synthetic bacterial community colonizes wheat roots grown in soil and mimics the assembly pattern of a field community
bioRxiv from @fredebak.bsky.social et al #NNF_INTERACT
www.biorxiv.org/content/10.1...
bioRxiv from @fredebak.bsky.social et al #NNF_INTERACT
www.biorxiv.org/content/10.1...
#microsky #phagesky #phage
Anyone who’s tried deleting prophages in the lab by HR knows the difficulties of the task. Here we have an example of how HR-mediated natural transformation might hit the same hurdle in a more native context.
academic.oup.com/mbe/article/...
Anyone who’s tried deleting prophages in the lab by HR knows the difficulties of the task. Here we have an example of how HR-mediated natural transformation might hit the same hurdle in a more native context.
academic.oup.com/mbe/article/...
Chromosomal Curing Drives an Arms Race Between Bacterial Transformation and Prophage
Abstract. Transformation occurs when bacteria import exogenous DNA via the competence machinery and integrate it into their genome through homologous recom
academic.oup.com
November 23, 2025 at 4:39 PM
#microsky #phagesky #phage
Anyone who’s tried deleting prophages in the lab by HR knows the difficulties of the task. Here we have an example of how HR-mediated natural transformation might hit the same hurdle in a more native context.
academic.oup.com/mbe/article/...
Anyone who’s tried deleting prophages in the lab by HR knows the difficulties of the task. Here we have an example of how HR-mediated natural transformation might hit the same hurdle in a more native context.
academic.oup.com/mbe/article/...
Reposted by Emmanuele Severi
Bacteriophage density influences the rate of resistance evolution.
DOI: 10.1101/2025.10.05.680490
https://pubmed.ncbi.nlm.nih.gov/41256655/
DOI: 10.1101/2025.10.05.680490
https://pubmed.ncbi.nlm.nih.gov/41256655/
Bacteriophage density influences the rate of resistance evolution - PubMed
The antagonistic relationship between bacteria and bacteriophages (phages) drives genetic changes that result in phage resistance. Phage resistance mutations arise in natural microbial communities and during the treatment of diseases with phages (phage therapy), making it important to understand the …
pubmed.ncbi.nlm.nih.gov
November 22, 2025 at 4:01 AM
Reposted by Emmanuele Severi
We are recruiting Academic Editors for our new journal, PLOS Aging and Health—a selective open science journal for research on developmental and age-related processes and changes across the life course.
➡️ Apply now: plos.io/48o1LQV
➡️ Apply now: plos.io/48o1LQV
November 21, 2025 at 7:01 PM
We are recruiting Academic Editors for our new journal, PLOS Aging and Health—a selective open science journal for research on developmental and age-related processes and changes across the life course.
➡️ Apply now: plos.io/48o1LQV
➡️ Apply now: plos.io/48o1LQV
Reposted by Emmanuele Severi
🚨Preprint alert - this is a big one! We transfer the revolutionary power of TnSeq to bacteriophages.
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
November 20, 2025 at 8:40 PM
🚨Preprint alert - this is a big one! We transfer the revolutionary power of TnSeq to bacteriophages.
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
Excited to share our new study! Great fun working again with my good friend @stephenrgarrett.bsky.social — always a highlight! Huge thanks to fantastic co-authors @samkaytucker.bsky.social, @drandyjroe.bsky.social and our star technician Vojtech Pavelka. academic.oup.com/nargab/artic...
November 21, 2025 at 6:27 PM
Reposted by Emmanuele Severi
Excited to share our new study! Great fun working again with my good friend @stephenrgarrett.bsky.social — always a highlight! Huge thanks to fantastic co-authors @samkaytucker.bsky.social, @drandyjroe.bsky.social and our star technician Vojtech Pavelka. academic.oup.com/nargab/artic...
November 21, 2025 at 5:45 PM
Excited to share our new study! Great fun working again with my good friend @stephenrgarrett.bsky.social — always a highlight! Huge thanks to fantastic co-authors @samkaytucker.bsky.social, @drandyjroe.bsky.social and our star technician Vojtech Pavelka. academic.oup.com/nargab/artic...
Reposted by Emmanuele Severi
Reposted by Emmanuele Severi
