An old timelapse I dug up... U937-derived macrophages bathed in green and red dextran... the green dextran is pH sensitive, leading to the appearance of mostly red lysosomes.

CellPose 3

MicroSam

SamCell results

For testing, I used these three DIC images (which I so far never segmented automatically and had to rely on manual ROI selection) - a tissue culture room microscope, an Incucyte image and a high-end confocal DIC channel

Very nice! During our practical course we once used the ColorfulCell vector for a similar purpose www.addgene.org/62449/

ER-lysosome contact sites (green) in MEFs on the background of an ER stain (grey, GFP-VAPA)