Would such a probe work for other organelles, eg mitochondria or endosomes, if tagged properly? Or does it need to be a continual network like ER has?

Hlasovaci listek si tisknem sami, prijde pry jen identifikacni listek.

Sléz velkokvětý nebo neco tomu podobneho. Kakosty maji trochu jiny tvar kvetu. Jiná čeleď :)

Sléz,ne?

Oh, I just add a few grams of RNase to every meal and let it incubate for a while.

I might have not used exactly the same images for all 3 tools, sorry for that - I did this from two different laptops and picked slightly different images somewhere.

Difficult to generalize but Cellpose was probably simplest to install even though I ran the remaining two from Google Colab. Low-res images were easier to segment with all 3 tools (cell boundaries are clearly visible). MicroSam surprisingly good even with the confocal but still not reliable.

CellPose 3

MicroSam

SamCell results

For testing, I used these three DIC images (which I so far never segmented automatically and had to rely on manual ROI selection) - a tissue culture room microscope, an Incucyte image and a high-end confocal DIC channel

play.google.com/store/apps/d... Try this it's superior to Google maps in terms of map layout

Very nice! During our practical course we once used the ColorfulCell vector for a similar purpose www.addgene.org/62449/

Very nice! During our practical course we once used the ColorfulCell vector for a similar purpose www.addgene.org/62449/

You an even go as far as to color-code each cell-level datapoint by the experimental run it came from, so the reader can see if there if the cell variability is clustered or dispersed.