I watch $MRNA and Moderna: bench scientist. LNP IVT mRNA with N1-methylpseudouridine and cap1 lifts translation; innate sensing, thermostability and delivery undervalued. focus/exposure. I use @bluestocks.app #finsky
December 7, 2025 at 8:05 PM
I watch $MRNA and Moderna: bench scientist. LNP IVT mRNA with N1-methylpseudouridine and cap1 lifts translation; innate sensing, thermostability and delivery undervalued. focus/exposure. I use @bluestocks.app #finsky
Recent review covers enzyme engineering advances for solvent & thermostability, using B-factors, ancestral methods & machine learning. #EnzymeEngineering #Biocatalysts
Details: doi.org/10.1016/j.bidere.2025.100005
Details: doi.org/10.1016/j.bidere.2025.100005
November 26, 2025 at 3:30 PM
Recent review covers enzyme engineering advances for solvent & thermostability, using B-factors, ancestral methods & machine learning. #EnzymeEngineering #Biocatalysts
Details: doi.org/10.1016/j.bidere.2025.100005
Details: doi.org/10.1016/j.bidere.2025.100005
## Hyper-Specific Sub-Field Selection & Research Topic Generation
**Randomly Selected Sub-Field:** Directed Evolution of Restriction Enzymes with Enhanced Sequence Specificity & Thermostability. **Combined Research Topic:** *Automated Design & Validation of Thermostable, Highly Specific…
**Randomly Selected Sub-Field:** Directed Evolution of Restriction Enzymes with Enhanced Sequence Specificity & Thermostability. **Combined Research Topic:** *Automated Design & Validation of Thermostable, Highly Specific…
## Hyper-Specific Sub-Field Selection & Research Topic Generation
**Randomly Selected Sub-Field:** Directed Evolution of Restriction Enzymes with Enhanced Sequence Specificity & Thermostability. **Combined Research Topic:** *Automated Design & Validation of Thermostable, Highly Specific Restriction Enzymes via a Multi-Modal Data Ingestion & Evaluation Pipeline for Precision Genome Editing.* --- ## Automated Design & Validation of Thermostable, Highly Specific Restriction Enzymes via a Multi-Modal Data Ingestion & Evaluation Pipeline for Precision Genome Editing…
freederia.com
November 25, 2025 at 8:31 AM
## Hyper-Specific Sub-Field Selection & Research Topic Generation
**Randomly Selected Sub-Field:** Directed Evolution of Restriction Enzymes with Enhanced Sequence Specificity & Thermostability. **Combined Research Topic:** *Automated Design & Validation of Thermostable, Highly Specific…
**Randomly Selected Sub-Field:** Directed Evolution of Restriction Enzymes with Enhanced Sequence Specificity & Thermostability. **Combined Research Topic:** *Automated Design & Validation of Thermostable, Highly Specific…
🧬Method Spotlight
MD simulations to understand the mechanism behind the enhanced thermostability of BrCas12b variant🧪
Read this insightful #OpenAccess article by Janani Sampath et al, 'Exploring the temperature stability of CRISPR-Cas12b using molecular dynamics simulations' doi.org/10.1039/D5ME...
MD simulations to understand the mechanism behind the enhanced thermostability of BrCas12b variant🧪
Read this insightful #OpenAccess article by Janani Sampath et al, 'Exploring the temperature stability of CRISPR-Cas12b using molecular dynamics simulations' doi.org/10.1039/D5ME...
Exploring the temperature stability of CRISPR-Cas12b using molecular dynamics simulations
The thermal stability of CRISPR-Cas nucleases is a critical factor for their successful application in ‘one-pot’ diagnostic assays that utilize high-temperature isothermal amplification. To understand...
doi.org
November 19, 2025 at 3:10 PM
🧬Method Spotlight
MD simulations to understand the mechanism behind the enhanced thermostability of BrCas12b variant🧪
Read this insightful #OpenAccess article by Janani Sampath et al, 'Exploring the temperature stability of CRISPR-Cas12b using molecular dynamics simulations' doi.org/10.1039/D5ME...
MD simulations to understand the mechanism behind the enhanced thermostability of BrCas12b variant🧪
Read this insightful #OpenAccess article by Janani Sampath et al, 'Exploring the temperature stability of CRISPR-Cas12b using molecular dynamics simulations' doi.org/10.1039/D5ME...
Novel Dual-Coenzyme Specificity and Thermostability of Malate Dehydrogenase Identified in the Cyanobacterium Microcystis aeruginosa PCC7806 www.mdpi.com/3555730
www.mdpi.com
November 18, 2025 at 4:04 PM
Novel Dual-Coenzyme Specificity and Thermostability of Malate Dehydrogenase Identified in the Cyanobacterium Microcystis aeruginosa PCC7806 www.mdpi.com/3555730
Although the thermo-instability of FGF-2 is well documented, the thermostability of other essential growth factors has not been investigated. This application note reviews the stability of Qkine growth factors under cell culture-like conditions > zurl.co/XG5cN
November 13, 2025 at 8:01 AM
Although the thermo-instability of FGF-2 is well documented, the thermostability of other essential growth factors has not been investigated. This application note reviews the stability of Qkine growth factors under cell culture-like conditions > zurl.co/XG5cN
These findings suggest that the enzyme's thermostability is not governed by a single dominant feature but rather by the cooperative contribution of multiple structural elements, making it a promising candidate for the bioremediation of organophosphate-contaminated water systems.
October 31, 2025 at 11:48 PM
These findings suggest that the enzyme's thermostability is not governed by a single dominant feature but rather by the cooperative contribution of multiple structural elements, making it a promising candidate for the bioremediation of organophosphate-contaminated water systems.
This study delves into the structural determinants and conformational adaptability that contribute to the exceptional thermostability of a paraoxonase enzyme from Bacillus sp. strain S3wahi.
October 31, 2025 at 11:48 PM
This study delves into the structural determinants and conformational adaptability that contribute to the exceptional thermostability of a paraoxonase enzyme from Bacillus sp. strain S3wahi.
Rich in atomic-environment signal, SLAE features outperform PLMs and task-specific models across diverse, challenging downstream tasks, including binding affinity, thermostability and chemical shift prediction. All-atom structure pretraining is all you need! (4/8)
October 9, 2025 at 5:36 PM
Rich in atomic-environment signal, SLAE features outperform PLMs and task-specific models across diverse, challenging downstream tasks, including binding affinity, thermostability and chemical shift prediction. All-atom structure pretraining is all you need! (4/8)
Crucial to the screening is the lab scale protein expression pipeline below.
We use an old trick beloved by the IDR-community for protein purication: Heat lysis.
Due the thermostability of the binders, we get ~95% pure lysate after a heating step in 96-well plates.
dx.doi.org/10.17504/pro...
We use an old trick beloved by the IDR-community for protein purication: Heat lysis.
Due the thermostability of the binders, we get ~95% pure lysate after a heating step in 96-well plates.
dx.doi.org/10.17504/pro...
Medium throughput protein expression and purification for protein design using heat lysis
This protocols describes medium throughput protein expression in E. coli using heat-lysis as a built in purification step. It is intended for preparation of heat-stable de novo...
dx.doi.org
September 15, 2025 at 7:51 PM
Crucial to the screening is the lab scale protein expression pipeline below.
We use an old trick beloved by the IDR-community for protein purication: Heat lysis.
Due the thermostability of the binders, we get ~95% pure lysate after a heating step in 96-well plates.
dx.doi.org/10.17504/pro...
We use an old trick beloved by the IDR-community for protein purication: Heat lysis.
Due the thermostability of the binders, we get ~95% pure lysate after a heating step in 96-well plates.
dx.doi.org/10.17504/pro...
Big news! Watchmaker Genomics + @promegacorporation.bsky.social are partnering to advance molecular analysis with a next-gen reverse transcriptase offering:
🔹 Thermostability
🔹 Inhibitor tolerance
🔹 High processivity
Setting a new benchmark for RNA analysis. Full details in comments.
🔹 Thermostability
🔹 Inhibitor tolerance
🔹 High processivity
Setting a new benchmark for RNA analysis. Full details in comments.
September 9, 2025 at 3:00 PM
Big news! Watchmaker Genomics + @promegacorporation.bsky.social are partnering to advance molecular analysis with a next-gen reverse transcriptase offering:
🔹 Thermostability
🔹 Inhibitor tolerance
🔹 High processivity
Setting a new benchmark for RNA analysis. Full details in comments.
🔹 Thermostability
🔹 Inhibitor tolerance
🔹 High processivity
Setting a new benchmark for RNA analysis. Full details in comments.
Unraveling the hidden costs of protein stabilization - how computational tools can boost thermostability but compromise solubility. Uncovering the molecular mechanisms behind this trade-o...
🧵 Thread below
Full analysis: https://helixbrief.com/article/544cc779-6c6a-498c-896f-288d9b27f828
🧵 Thread below
Full analysis: https://helixbrief.com/article/544cc779-6c6a-498c-896f-288d9b27f828
September 9, 2025 at 9:52 AM
Unraveling the hidden costs of protein stabilization - how computational tools can boost thermostability but compromise solubility. Uncovering the molecular mechanisms behind this trade-o...
🧵 Thread below
Full analysis: https://helixbrief.com/article/544cc779-6c6a-498c-896f-288d9b27f828
🧵 Thread below
Full analysis: https://helixbrief.com/article/544cc779-6c6a-498c-896f-288d9b27f828
Moderna nails mRNA/LNP PKPD; I focus on $MRNA mispriced-40% rerate, TLR7/8+thermostability.
September 7, 2025 at 1:08 PM
Moderna nails mRNA/LNP PKPD; I focus on $MRNA mispriced-40% rerate, TLR7/8+thermostability.
Moderna blends nucleoside-modified mRNA and LNP delivery to control innate sensing (TLR7/8), biodistribution and PK/PD. Watching $MRNA: translatonal risk underpriced, 40% rerate probable as thermostability & delivery improve. Focus/expsure
September 6, 2025 at 7:08 PM
Moderna blends nucleoside-modified mRNA and LNP delivery to control innate sensing (TLR7/8), biodistribution and PK/PD. Watching $MRNA: translatonal risk underpriced, 40% rerate probable as thermostability & delivery improve. Focus/expsure
$MRNA interests me. Moderna pairs nucleoside‑modified mRNA + LNP/saRNA; TLR7/8 sensing, biodistribution & thermostability set PK/PD. Market misprices translatonal risk - 40% rerate 12m. Focus/exposureefficency
September 6, 2025 at 3:05 PM
$MRNA interests me. Moderna pairs nucleoside‑modified mRNA + LNP/saRNA; TLR7/8 sensing, biodistribution & thermostability set PK/PD. Market misprices translatonal risk - 40% rerate 12m. Focus/exposureefficency
Combining experimental enzyme engineering with MD at elevated temperature, this study identifies hotspots in choline oxidase and proposes substitutions that reduce fluctuations while preserving active-site geometry linking residue-level dynamics to thermostability gains. doi.org/10.1080/0739...
August 27, 2025 at 2:08 PM
Combining experimental enzyme engineering with MD at elevated temperature, this study identifies hotspots in choline oxidase and proposes substitutions that reduce fluctuations while preserving active-site geometry linking residue-level dynamics to thermostability gains. doi.org/10.1080/0739...
Four recent JBSD Vol. 43(11) articles on biomolecular structure & dynamic focusing on helix stability, protein–protein interfaces, enzyme thermostability, and nanoscale biointerfaces. 1/5
August 27, 2025 at 12:47 PM
Four recent JBSD Vol. 43(11) articles on biomolecular structure & dynamic focusing on helix stability, protein–protein interfaces, enzyme thermostability, and nanoscale biointerfaces. 1/5
🔗 The pipeline allows to identify distal mutation hotspots—sites distant from the active site, that can still influence enzyme functional properties (e.g. activity and thermostability)
August 8, 2025 at 10:43 AM
🔗 The pipeline allows to identify distal mutation hotspots—sites distant from the active site, that can still influence enzyme functional properties (e.g. activity and thermostability)
We tested 2 green solvents (GVL and IPP) to see if they could help break down plastic textiles in a way that makes them easier to recycle with enzymes.
Key findings
GVL reduces enzyme thermostability
2/4
Key findings
GVL reduces enzyme thermostability
2/4
August 3, 2025 at 2:17 PM
We tested 2 green solvents (GVL and IPP) to see if they could help break down plastic textiles in a way that makes them easier to recycle with enzymes.
Key findings
GVL reduces enzyme thermostability
2/4
Key findings
GVL reduces enzyme thermostability
2/4
Xiangwen Wang, Gaojie Jin, Xiaowei Huang, Ronghui Mu: ThermoRL:Structure-Aware Reinforcement Learning for Protein Mutation Design to Enhance Thermostability https://arxiv.org/abs/2507.18816 https://arxiv.org/pdf/2507.18816 https://arxiv.org/html/2507.18816
July 28, 2025 at 6:29 AM
Xiangwen Wang, Gaojie Jin, Xiaowei Huang, Ronghui Mu: ThermoRL:Structure-Aware Reinforcement Learning for Protein Mutation Design to Enhance Thermostability https://arxiv.org/abs/2507.18816 https://arxiv.org/pdf/2507.18816 https://arxiv.org/html/2507.18816
PDBe is at #ISMBECCB2025!
Structural Bioinformaticians & Computational Biologist sharing insight & inc. research on:
...predicting protein thermostability change upon mutation...
📖 OrgNet: pubmed.ncbi.nlm.nih.gov/40662839/
Conference Abstracts:
🔗 www.iscb.org/ismbeccb2025...
@iscb.bsky.social
Structural Bioinformaticians & Computational Biologist sharing insight & inc. research on:
...predicting protein thermostability change upon mutation...
📖 OrgNet: pubmed.ncbi.nlm.nih.gov/40662839/
Conference Abstracts:
🔗 www.iscb.org/ismbeccb2025...
@iscb.bsky.social
The third oral presentation in #3DSIG with Petr Popov: "OrgNet: Orientation-gnostic protein stability assessment using convolutional neural networks." #ISMBECCB2025 @iscb.bsky.social
Following this presentation, we'll break for lunch 🍲 and return shortly after.
Following this presentation, we'll break for lunch 🍲 and return shortly after.
July 22, 2025 at 1:28 PM
PDBe is at #ISMBECCB2025!
Structural Bioinformaticians & Computational Biologist sharing insight & inc. research on:
...predicting protein thermostability change upon mutation...
📖 OrgNet: pubmed.ncbi.nlm.nih.gov/40662839/
Conference Abstracts:
🔗 www.iscb.org/ismbeccb2025...
@iscb.bsky.social
Structural Bioinformaticians & Computational Biologist sharing insight & inc. research on:
...predicting protein thermostability change upon mutation...
📖 OrgNet: pubmed.ncbi.nlm.nih.gov/40662839/
Conference Abstracts:
🔗 www.iscb.org/ismbeccb2025...
@iscb.bsky.social
The recent article by Walunjkar et al. on the evolution of protein thermostability is the focus of this month's Highlight feature.
Highlight: Cellular Environment and Protein Structure Help Organisms Cope with Temperature Stress
🔗 doi.org/10.1093/molbev/msaf155
#evobio #molbio
Highlight: Cellular Environment and Protein Structure Help Organisms Cope with Temperature Stress
🔗 doi.org/10.1093/molbev/msaf155
#evobio #molbio
July 18, 2025 at 10:53 PM
The recent article by Walunjkar et al. on the evolution of protein thermostability is the focus of this month's Highlight feature.
Highlight: Cellular Environment and Protein Structure Help Organisms Cope with Temperature Stress
🔗 doi.org/10.1093/molbev/msaf155
#evobio #molbio
Highlight: Cellular Environment and Protein Structure Help Organisms Cope with Temperature Stress
🔗 doi.org/10.1093/molbev/msaf155
#evobio #molbio
Something I'm working on:
Running a protein language model trained on evolutionary data, and forcing it to overrun until it hallucinates, looking into why hallucinations happen
Model optimises for thermostability often, and it seems to think adding prolines is an easy way to do so
Running a protein language model trained on evolutionary data, and forcing it to overrun until it hallucinates, looking into why hallucinations happen
Model optimises for thermostability often, and it seems to think adding prolines is an easy way to do so
July 7, 2025 at 10:16 AM
Something I'm working on:
Running a protein language model trained on evolutionary data, and forcing it to overrun until it hallucinates, looking into why hallucinations happen
Model optimises for thermostability often, and it seems to think adding prolines is an easy way to do so
Running a protein language model trained on evolutionary data, and forcing it to overrun until it hallucinates, looking into why hallucinations happen
Model optimises for thermostability often, and it seems to think adding prolines is an easy way to do so
We find that it works excellently with “boiling lysis” – exploiting the high thermostability of binder designs to remove endogenous proteins – and then simply dilution the supernatant with buffer before measurement.
June 19, 2025 at 6:45 AM
We find that it works excellently with “boiling lysis” – exploiting the high thermostability of binder designs to remove endogenous proteins – and then simply dilution the supernatant with buffer before measurement.
#Medsky🧪 #iDsky #Immunosky #pharmasky #publichealth #Drugsdelivery #mRNA #Vaccine Technology & Beyond #Covid19
www.mdpi.com/2076-393X/13...
www.mdpi.com/2076-393X/13...
June 3, 2025 at 10:47 AM
#Medsky🧪 #iDsky #Immunosky #pharmasky #publichealth #Drugsdelivery #mRNA #Vaccine Technology & Beyond #Covid19
www.mdpi.com/2076-393X/13...
www.mdpi.com/2076-393X/13...