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Reposted by Davide Angeletti

Manuel Kleiner @manuelkleiner.bsky.social · Aug 7

I am really excited to see this article on basic experimental design principles published. Definitely will be mandatory reading for incoming graduate students in my lab. Maggie Wagner who led this article did an amazing job with the illustrations of basic principles www.nature.com/articles/s41...

How thoughtful experimental design can empower biologists in the omics era - Nature Communications

Here, the authors discuss principles of experimental design that are relevant for all biology research, along with special considerations for projects using -omics approaches, highlighting common expe...

www.nature.com

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Reposted by Davide Angeletti

C.A.M(Xander)_de_Haan.bsky.social @xdh.bsky.social · Jul 29

New review paper from the influenza group
@utrechtvirology.bsky.social
together with Mengying Liu and Erik de Vries on virion motility of sialoglycan-cleaving respiratory viruses through mucus and on the surface cells in NPJ viruses @natureportfolio.nature.com
doi.org/10.1038/s442...

Virion motility of sialoglycan-cleaving respiratory viruses - npj Viruses

npj Viruses - Virion motility of sialoglycan-cleaving respiratory viruses

doi.org

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Reposted by Davide Angeletti

Waggoner Lab @labwaggoner.bsky.social · Jul 19

Preprint: Local B-cell immunity and durable memory following live-attenuated influenza intranasal vaccination of humans @profshanecrotty.bsky.social @hannahdstacey.bsky.social
www.biorxiv.org/content/10.1...

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Reposted by Davide Angeletti

Gabriel Victora @victora.bsky.social · Jun 5

Wanted to highlight our latest preprint--a huge effort by multiple people and labs, but led primarily by @wsdewitt.github.io, Tatsuya Araki, and Ashni Vora, in a very close wet-dry collaboration with @matsen.bsky.social’s lab at the Hutch

www.biorxiv.org/content/10.1...

Replaying germinal center evolution on a quantified affinity landscape

Darwinian evolution of immunoglobulin genes within germinal centers (GC) underlies the progressive increase in antibody affinity following antigen exposure. Whereas the mechanics of how competition be...

www.biorxiv.org

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Reposted by Davide Angeletti

Carolyn King @immunologyking.bsky.social · May 30

Last chance TODAY - to register and submit abstracts for #EMBObarrierTissues in Basel 🇨🇭🇨🇭🇨🇭! We've hit our registration goal 🥳, but there's always room for more. Join @maurogaya.bsky.social and me to explore the latest in lymphocyte and mucosal immunology. Details: meetings.embo.org/event/25-bar...

Adaptive immunity in barrier tissues

Immune responses lie at the heart of almost every aspect of human health, including host responses to infection, autoimmunity, cancer, metabolism and aging. While adaptive immune responses by B and T…

meetings.embo.org

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Davide Angeletti @angelettilab.bsky.social · May 29

Congrats Danica 😀

Davide Angeletti @angelettilab.bsky.social · May 29

Very proud of Danica Besavilla, PhD student in the lab, receiving the Poster award at the #Antibodies & Complement meeting, taking place in Catania.
She presented her paper on mAbs to #Influenza #Neuraminidase , just published in @natureportfolio.nature.com #npjViruses
www.nature.com/articles/s44...

Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase - npj Viruses

npj Viruses - Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase

www.nature.com

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Reposted by Davide Angeletti

Moffitt Lab @moffittlab.bsky.social · May 28

Proud to announce our new technique for tracking relative B cell clonality in situ with B Cell Receptor MERFISH (BCR-MERFISH)! Congratulations to Evan Yang and our colleagues in the Carroll laboratory! Check out our bioRxiv: www.biorxiv.org/content/10.1... 1/9

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Reposted by Davide Angeletti

Michelle Linterman @linterman.bsky.social · May 22

Our @immunolcellbiol.bsky.social "Highlights of 2024: Germinal Centers" is now out. Led by Theresa Pankhurst, we discuss some (there's never enough space for all!) neat germinal centre papers that were published in 2024, and whose findings are moving the field forward. doi.org/10.1111/imcb...

<em>Immunology & Cell Biology</em> | ASI Journal | Wiley Online Library

In this article for the Highlights of 2024 series, we review the latest advances in the biology of the germinal center response. These discoveries provide key insights into germinal center function a...

doi.org

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Reposted by Davide Angeletti

Eduardo Villablanca @ejvillablanca.bsky.social · May 9

Postdoctoral position in Computational Biology open !

Our Lab is trying to understand the molecular and cellular mechanisms involved in establishing intestinal immune homeostasis and how a breakdown of these mechanisms may lead to diseases

More info 👇 :

lnkd.in/dQZq55nv

lnkd.in/d6sbw59P

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Reposted by Davide Angeletti

ASM @asm.org · May 6

The American Society for Microbiology is committed to ensuring that scientific research is conducted adhering to strict standards for biosafety and biosecurity, but pausing research is not the path forward. Read our statement: asm.org/press-releas...

Pausing Research Not a Path to Better Biosafety & Biosecurity

The American Society for Microbiology is committed to ensuring that scientific research is conducted adhering to strict standards for biosafety and biosecurity.

asm.org

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Reposted by Davide Angeletti

Mauro Gaya @maurogaya.bsky.social · Apr 30

🚨🦠🔬 Last month to register to the EMBO workshop on Adaptive Immunity in Barrier Tissues.

📍 Where: Basel, Switzerland
📅 When: 26th–29th August 2025
📝 Registration deadline: 30th May 2025

Childcare support & travel grants from @embo.org and @efis-immunology.bsky.social

#EMBOBarrierTissues

Adaptive immunity in barrier tissues

Immune responses lie at the heart of almost every aspect of human health, including host responses to infection, autoimmunity, cancer, metabolism and aging. While adaptive immune responses by B and T…

meetings.embo.org

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Reposted by Davide Angeletti

Rockefeller University Press @rupress.org · Apr 30

In @jem.org, Conlon, Huang, and Gerner show that immunization generates concentration gradients of antigens and #inflammation across interconnected chains of lymph nodes that regulate the magnitude and heterogeneity of adaptive immunity. rupress.org/jem/article/...

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Reposted by Davide Angeletti

Michelle Linterman @linterman.bsky.social · Apr 27

Neat @pnas.org paper from @angelettilab.bsky.social & @matsbemark.bsky.social on ectopic germinal centres in the nasal turbinates of mice and humans. doi.org/10.1073/pnas...

Ectopic germinal centers in the nasal turbinates contribute to B cell immunity to intranasal viral infection and vaccination | PNAS

The nasal mucosa is the first immunologically active site that respiratory viruses encounter and establishing immunity at the initial point of path...

doi.org

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Reposted by Davide Angeletti

Waggoner Lab @labwaggoner.bsky.social · Mar 27

Ectopic germinal centers in the nasal turbinates contribute to B cell immunity to intranasal viral infection and vaccination @angelettilab.bsky.social
www.pnas.org/doi/10.1073/...

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Davide Angeletti @angelettilab.bsky.social · Mar 25

Thank you, Marty!

Davide Angeletti @angelettilab.bsky.social · Mar 25

Thank you! This work opened up a lot of questions, which we hope to answer in future studies

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Davide Angeletti @angelettilab.bsky.social · Mar 25

Thank you @maurogaya.bsky.social , looking forward to the meeting!

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Davide Angeletti @angelettilab.bsky.social · Mar 25

And I forgot to thank the collaborators, without whom the work would have not been possible.
@matsbemark.bsky.social for continued discussions and key scientific input.
#KaWeiTang for swabs #JohanHellgren and team for tonsils
Other lab members: #NimithaMathew , #LauraReusch and #KarinSchön above all

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Davide Angeletti @angelettilab.bsky.social · Mar 25

We show that the same structures are also present at steady state, after vaccination and also in humans.
Our work adds to recent studies that identified DALT, nasal immunity and ectopic GC in skin.

We thank the funders @vetenskapsradet.bsky.social , @kawresearch.bsky.social and @erc.europa.eu !

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Davide Angeletti @angelettilab.bsky.social · Mar 25

Interestingly, another old paper described M cells in mouse nasal turbinates.
By sequencing we showed that germinal centers in nasal tissue were essentially transcriptionally identical, but clonally distinct, to those in cervical lymph nodes and NALT. (5/N)

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Davide Angeletti @angelettilab.bsky.social · Mar 25

But we found some old studies describing nasal tissue immunity after influenza infection in absence of NALT and cervical lymph nodes. Indeed, we could confirm ectopic germinal centers in the nasal turbinates by microscopy. Cells express AID, we also found Tfh and FDCs. (4/N)

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Davide Angeletti @angelettilab.bsky.social · Mar 25

By flow cytometry, a lot of these cells looked like germinal center B cells and followed the same dynamics as those in secondary lymphoid organs (and were antigen specific).
We were confused because we had carefully removed the NALTs before running them. (3/N)

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Davide Angeletti @angelettilab.bsky.social · Mar 25

First, Romain showed that by infecting with higher viral titer in lower volume we can increase viral replication in nasal tissue and also had more B cells within the cervical lymph node and nasal tissue itself (2/N)

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Davide Angeletti @angelettilab.bsky.social · Mar 25

Our latest study by PhD student #RomainGailleton shows that upon #influenza infection, #Bcell can respond rapidly by forming ectopic #GerminalCenter within the nasal tissue.
The work has just been published in @pnas.org (1/N)

www.pnas.org/doi/10.1073/...

Ectopic germinal centers in the nasal turbinates contribute to B cell immunity to intranasal viral infection and vaccination | PNAS

The nasal mucosa is the first immunologically active site that respiratory viruses encounter and establishing immunity at the initial point of path...

www.pnas.org

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