Virus discovery has accelerated but linking viruses to hosts remains challenging. This study uses synthetic microbiomes to optimize and benchmark Hi-C for virus-host linkage inference, and applies thi...

Cells regulate metabolic fluxes to balance energy production, biosynthesis, and the efficient use of limited resources, including the finite capacity for synthesizing and maintaining metabolic enzymes...

Nature Climate Change - Microbes wake up

AbstractMotivation. Over the past years, substantial numbers of microbial species’ genomes have been deposited outside of conventional INSDC databases.Resu

The upland soil microbiome is dominated by aerobic bacteria that oxidize atmospheric trace gases, including CO, H2, and CH4. As a result, soils are the largest biological sink for these climate-active...

Biogenic methane, the largest contributor to atmospheric methane, is produced via different microbial methanogenic pathways, depending on the substrates and type of methanogens. Stable carbon and hydr...

Mathematical modelling and experimental tests reveal principles that govern displacement of a resident strain by an invader in microbial communities.

An approach combining BONCAT, stable isotope probing and metaproteomics showcases the hidden metabolic interconnectivity of microorganisms within an anaerobic digestion community.

Abstract. The order Pelagibacterales (SAR11) is the most abundant group of heterotrophic bacteria in the global surface ocean, where individual sublineages

Diatoms are a highly diverse group of phytoplankton that have a large impact on global primary production and carbon sequestration in the ocean. However, they are evolutionarily divergent from model p...

Nitrous oxide (N2O) is a serious concern due to its role in global warming and ozone destruction. Agricultural practices account for ∼80% of all anthropogenic N2O produced in the US, due in large part to the stimulation of microbial denitrification. Stable isotopes are uniquely suited to examine both microbial N2O sources and the mechanism of N2O biosynthesis through the use of Site Preference (δ15NSP; the difference in δ15N between the central and outer N atoms in N2O) and kinetic isotope effects (KIEs), respectively. Using trace gas isotope ratio mass spectrometry (TG-IRMS), we determined the δ15N, δ15Nα, δ15Nβ, and δ18O of N2O produced by a purified cytochrome c nitric oxide reductase (cNOR) from Paracoccus denitrificans. We also calculated δ15NSP, the KIEs, and associated isotopic enrichment factors (ε) for Nbulk, Nα, and Nβ. A normal isotope effect was observed for bulk 15N, with a KIE value of 1.0086 ± 0.0009 (ε = −8.6 ± 0.9‰). The isotope effects for both 15Nα and 15Nβ were also normal, with position-specific KIEs of 1.0072 ± 0.0010 (ε = −7.2 ± 1.0‰) and 1.0100 ± 0.0010 (ε = −9.9 ± 1.0‰), respectively, and δ15NSP values ranged from 0.5 to 8.7‰ with no significant trend as the reaction proceeded. Values of δ18O increased with N2O production (slope of δ18O against [−f ln f/(1 – f)] = −19.9 ± 1.9‰). We present implications for the mechanism of N2O production from cNOR based on our data.

Application deadline: 17 November 2025 at 23:59 hours local Danish time

The urea to ammonium concentration ratio is a good predictor of the relative importance of urea and ammonium to nitrite and N2O production in the ocean. In addition, the urea to ammonium ratio may be...

Dynamic microbial activity transforms environments and impacts health and disease in associations with plants and animals, including humans. Identifying the contribution of individual microbes to thos...

Single species tests on surrogate organisms from different trophic levels constitute a cornerstone in aquatic and terrestrial ecotoxicology, representing a major tool for assessing pesticide ecotoxici...