Eloïse Bertiaux
@ebertiaux.bsky.social
Reposted by Eloïse Bertiaux
A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social.
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...
December 25, 2025 at 1:41 PM
A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social.
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...
Reposted by Eloïse Bertiaux
As a Christmas present, our Jove paper with @ebertiaux.bsky.social is out and available for everyone ! Check this out & thanks @centriolelab.bsky.social for the nice highlight
A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social.
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...
December 25, 2025 at 3:03 PM
As a Christmas present, our Jove paper with @ebertiaux.bsky.social is out and available for everyone ! Check this out & thanks @centriolelab.bsky.social for the nice highlight
Reposted by Eloïse Bertiaux
How does C2CD3 contribute to distal appendage formation in centrioles? @ebertiaux.bsky.social @centriolelab.bsky.social show that C2CD3 architecturally organizes the distal #centriole, bridging the luminal distal ring complex & peripheral appendage sites @plosbiology.org 🧪 plos.io/496XZuH
December 10, 2025 at 2:02 PM
How does C2CD3 contribute to distal appendage formation in centrioles? @ebertiaux.bsky.social @centriolelab.bsky.social show that C2CD3 architecturally organizes the distal #centriole, bridging the luminal distal ring complex & peripheral appendage sites @plosbiology.org 🧪 plos.io/496XZuH
Reposted by Eloïse Bertiaux
Happy to share our fun team-up with @centriolelab.bsky.social & @stearnslab.bsky.social to reveal the architecture of the mammalian centriole’s Distal Ring 🛟. Very cool multiscale integration of #UExM 📏🔬with #CryoET ❄️🔬. Congrats to @ebertiaux.bsky.social & @computingcaitie.bsky.social #TeamTomo 🧪
How does C2CD3 contribute to distal appendage formation in centrioles? @centriolelab.bsky.social show that C2CD3 acts as a central architectural organizer of the distal #centriole, bridging the luminal distal ring complex & peripheral appendage sites @plosbiology.org 🧪 plos.io/496XZuH
December 10, 2025 at 11:02 AM
Happy to share our fun team-up with @centriolelab.bsky.social & @stearnslab.bsky.social to reveal the architecture of the mammalian centriole’s Distal Ring 🛟. Very cool multiscale integration of #UExM 📏🔬with #CryoET ❄️🔬. Congrats to @ebertiaux.bsky.social & @computingcaitie.bsky.social #TeamTomo 🧪
Reposted by Eloïse Bertiaux
🥳 Our new paper on C2CD3 is out in @plosbiology.org
With the labs of @cellarchlab.com , @chgenoud.bsky.social , and @stearnslab.bsky.social , we revealed the mammalian centriole Distal Ring architecture by combining #UExM and in situ #CryoET.
Big congrats to @ebertiaux.bsky.social and all involved.
With the labs of @cellarchlab.com , @chgenoud.bsky.social , and @stearnslab.bsky.social , we revealed the mammalian centriole Distal Ring architecture by combining #UExM and in situ #CryoET.
Big congrats to @ebertiaux.bsky.social and all involved.
How does C2CD3 contribute to distal appendage formation in centrioles? @centriolelab.bsky.social show that C2CD3 acts as a central architectural organizer of the distal #centriole, bridging the luminal distal ring complex & peripheral appendage sites @plosbiology.org 🧪 plos.io/496XZuH
December 10, 2025 at 12:36 PM
🥳 Our new paper on C2CD3 is out in @plosbiology.org
With the labs of @cellarchlab.com , @chgenoud.bsky.social , and @stearnslab.bsky.social , we revealed the mammalian centriole Distal Ring architecture by combining #UExM and in situ #CryoET.
Big congrats to @ebertiaux.bsky.social and all involved.
With the labs of @cellarchlab.com , @chgenoud.bsky.social , and @stearnslab.bsky.social , we revealed the mammalian centriole Distal Ring architecture by combining #UExM and in situ #CryoET.
Big congrats to @ebertiaux.bsky.social and all involved.
Thrilled to share that my postdoc paper has just been a published in @plosbiology.org !
Thank you to all co-authors, and a special thanks to Paul and Virginie @centriolelab.bsky.social for their support throughout this work.
Proud of what we achieved together!
journals.plos.org/plosbiology/...
Thank you to all co-authors, and a special thanks to Paul and Virginie @centriolelab.bsky.social for their support throughout this work.
Proud of what we achieved together!
journals.plos.org/plosbiology/...
The luminal ring protein C2CD3 acts as a radial in-to-out organizer of the distal centriole and appendages
The centriolar protein C2CD3 has been suggested to be crucial for distal appendage formation in centrioles, but its precise mechanistic role remains unclear. Using a combination of ultrastructure expa...
journals.plos.org
December 10, 2025 at 8:29 AM
Thrilled to share that my postdoc paper has just been a published in @plosbiology.org !
Thank you to all co-authors, and a special thanks to Paul and Virginie @centriolelab.bsky.social for their support throughout this work.
Proud of what we achieved together!
journals.plos.org/plosbiology/...
Thank you to all co-authors, and a special thanks to Paul and Virginie @centriolelab.bsky.social for their support throughout this work.
Proud of what we achieved together!
journals.plos.org/plosbiology/...
Reposted by Eloïse Bertiaux
Our latest T7SS study is now out in Science Advances!
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system
Cryo-EM structure reveals how a ubiquitin-like protein orchestrates assembly of a bacterial weapon system.
www.science.org
November 21, 2025 at 7:05 PM
Our latest T7SS study is now out in Science Advances!
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery.
www.science.org/doi/10.1126/...
Honored to receive the FEBS Booster Funds 2025.
My sincere thanks to FEBS for this recognition and their support of early-career researchers.
This funding will greatly help advance my work.
Congratulations as well to all fellow 2025 awardees.
My sincere thanks to FEBS for this recognition and their support of early-career researchers.
This funding will greatly help advance my work.
Congratulations as well to all fellow 2025 awardees.
November 18, 2025 at 12:54 PM
Honored to receive the FEBS Booster Funds 2025.
My sincere thanks to FEBS for this recognition and their support of early-career researchers.
This funding will greatly help advance my work.
Congratulations as well to all fellow 2025 awardees.
My sincere thanks to FEBS for this recognition and their support of early-career researchers.
This funding will greatly help advance my work.
Congratulations as well to all fellow 2025 awardees.
Reposted by Eloïse Bertiaux
We hire!
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
October 14, 2025 at 1:50 PM
We hire!
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
Reposted by Eloïse Bertiaux
The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6
October 13, 2025 at 12:55 PM
The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6
Reposted by Eloïse Bertiaux
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
August 28, 2025 at 7:03 AM
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Reposted by Eloïse Bertiaux
Be ready for an exciting meeting!
Save the date!
March 2027 on beautiful lake Maggiore right outside #Milan, are you ready for #Cilia2027? 🥳
Stay tuned for more info!
March 2027 on beautiful lake Maggiore right outside #Milan, are you ready for #Cilia2027? 🥳
Stay tuned for more info!
July 29, 2025 at 10:12 AM
Be ready for an exciting meeting!
Reposted by Eloïse Bertiaux
🚨 New EMBO Practical Course!
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social
💡 Register your interest to get notified when registration opens:
🔗 www.embl.org/about/info/c...
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social
💡 Register your interest to get notified when registration opens:
🔗 www.embl.org/about/info/c...
July 21, 2025 at 9:07 AM
🚨 New EMBO Practical Course!
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social
💡 Register your interest to get notified when registration opens:
🔗 www.embl.org/about/info/c...
Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬
📅 20–24 Apr 2026 | 📍 EMBL Heidelberg
Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social
💡 Register your interest to get notified when registration opens:
🔗 www.embl.org/about/info/c...
Reposted by Eloïse Bertiaux
💼 PostDoc position (2 years) on Trypanosoma brucei | Bordeaux, France | Lab MFP with Dr. Frédéric Bringaud @univbordeaux.bsky.social @cnrsaquitaine.bsky.social
🔬 Glycerol and differentiation in Trypanosoma brucei
🗓️ Deadline: September 15.
Start: by November 2025
labex-parafrap.fr/fr/emplois/4...
🔬 Glycerol and differentiation in Trypanosoma brucei
🗓️ Deadline: September 15.
Start: by November 2025
labex-parafrap.fr/fr/emplois/4...
June 10, 2025 at 9:15 AM
💼 PostDoc position (2 years) on Trypanosoma brucei | Bordeaux, France | Lab MFP with Dr. Frédéric Bringaud @univbordeaux.bsky.social @cnrsaquitaine.bsky.social
🔬 Glycerol and differentiation in Trypanosoma brucei
🗓️ Deadline: September 15.
Start: by November 2025
labex-parafrap.fr/fr/emplois/4...
🔬 Glycerol and differentiation in Trypanosoma brucei
🗓️ Deadline: September 15.
Start: by November 2025
labex-parafrap.fr/fr/emplois/4...
Reposted by Eloïse Bertiaux
On Friday, Daniel left us after more than 4 happy years in the lab @pasteur.fr and a great paper published today in @jcellsci.bsky.social as well as other contributions!
Many thanks for everything!
journals.biologists.com/jcs/article-...
Many thanks for everything!
journals.biologists.com/jcs/article-...
July 7, 2025 at 12:36 PM
On Friday, Daniel left us after more than 4 happy years in the lab @pasteur.fr and a great paper published today in @jcellsci.bsky.social as well as other contributions!
Many thanks for everything!
journals.biologists.com/jcs/article-...
Many thanks for everything!
journals.biologists.com/jcs/article-...
Reposted by Eloïse Bertiaux
#Trypanosoma, fixed, expanded, and imaged with #STED, stained for tubulin. Scale bar refers to expanded specimen, original structures are 3-3x smaller.
Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France
#fluorescencefriday #ExM #STEDmicroscopy
Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France
#fluorescencefriday #ExM #STEDmicroscopy
June 27, 2025 at 9:31 AM
#Trypanosoma, fixed, expanded, and imaged with #STED, stained for tubulin. Scale bar refers to expanded specimen, original structures are 3-3x smaller.
Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France
#fluorescencefriday #ExM #STEDmicroscopy
Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France
#fluorescencefriday #ExM #STEDmicroscopy
Reposted by Eloïse Bertiaux
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
June 19, 2025 at 8:58 AM
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Reposted by Eloïse Bertiaux
Honoured to have been introduced today by former lab member @ebertiaux.bsky.social at the annual Microbiology day of New Aquitaine. My first talk in a (former) church!
May 27, 2025 at 8:01 PM
Honoured to have been introduced today by former lab member @ebertiaux.bsky.social at the annual Microbiology day of New Aquitaine. My first talk in a (former) church!
Reposted by Eloïse Bertiaux
#Phosphofructokinase (PFK) is considered an irreversible glycolytic #enzyme. This study shows that #Trypanosomes are the only known organism to use PFK in the gluconeogenic direction under normal conditions, likely due to its compartmentalization within glycosomes @plosbiology.org 🧪 plos.io/43r84Qc
May 19, 2025 at 9:09 AM
#Phosphofructokinase (PFK) is considered an irreversible glycolytic #enzyme. This study shows that #Trypanosomes are the only known organism to use PFK in the gluconeogenic direction under normal conditions, likely due to its compartmentalization within glycosomes @plosbiology.org 🧪 plos.io/43r84Qc
Reposted by Eloïse Bertiaux
Huge congratulations to @marinelap.bsky.social for receiving the 2025 Young Researcher Award! 🏆👏 sbcf.fr/en/newslette...
March 19, 2025 at 8:03 AM
Huge congratulations to @marinelap.bsky.social for receiving the 2025 Young Researcher Award! 🏆👏 sbcf.fr/en/newslette...
Reposted by Eloïse Bertiaux
Brilliant work with unprecedented level of resolution of the trypanosome axoneme!
Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms | Science www.science.org/doi/10.1126/...
Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms
The flagellum of Trypanosoma brucei drives the parasite’s characteristic screw-like motion and is essential for its replication, transmission, and pathogenesis. However, the molecular details of this ...
www.science.org
March 13, 2025 at 9:00 PM
Brilliant work with unprecedented level of resolution of the trypanosome axoneme!
Reposted by Eloïse Bertiaux
Assistant prof / MCF post available in ProParaCyto.
March 7, 2025 at 8:17 AM
Assistant prof / MCF post available in ProParaCyto.
Reposted by Eloïse Bertiaux
March #ImageOfTheMonth goes to M.Bonhivers from MFP lab (UMR 5234).
Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.
Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.
March 3, 2025 at 11:00 AM
March #ImageOfTheMonth goes to M.Bonhivers from MFP lab (UMR 5234).
Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.
Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.
Reposted by Eloïse Bertiaux
A massive thanks to @bladerlab.bsky.social for the opportunity to put this mSphere of influence article together!
Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool!
journals.asm.org/doi/10.1128/...
Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool!
journals.asm.org/doi/10.1128/...
mSphere of Influence: The ever-expanding universe of parasite cell biology | mSphere
Single-celled apicomplexan parasites have to grow, replicate, and divide all of the
organelles of a eukaryotic cell all while being a tiny fraction of the size of most
other eukaryotes. For example, a...
journals.asm.org
March 4, 2025 at 10:01 PM
A massive thanks to @bladerlab.bsky.social for the opportunity to put this mSphere of influence article together!
Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool!
journals.asm.org/doi/10.1128/...
Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool!
journals.asm.org/doi/10.1128/...
Reposted by Eloïse Bertiaux
Thanks to a new mass spec pipeline for analysis of the #tubulin tail, the team of Julia Chamot-Rooke and Mariette Matondo revealed a few surprises for #trypanosome beta-tubulin:
-a new glutamylation site
-far less exhaustive detyrosination
Preprint here:
www.biorxiv.org/content/10.1...
-a new glutamylation site
-far less exhaustive detyrosination
Preprint here:
www.biorxiv.org/content/10.1...
March 4, 2025 at 8:07 AM
Thanks to a new mass spec pipeline for analysis of the #tubulin tail, the team of Julia Chamot-Rooke and Mariette Matondo revealed a few surprises for #trypanosome beta-tubulin:
-a new glutamylation site
-far less exhaustive detyrosination
Preprint here:
www.biorxiv.org/content/10.1...
-a new glutamylation site
-far less exhaustive detyrosination
Preprint here:
www.biorxiv.org/content/10.1...