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Lorenz Grundmann @lgrundmann.bsky.social · 1h

... or a 30S proteasome.

Lorenz Grundmann @lgrundmann.bsky.social · 1h

The last one looks like a 26S proteasome (~350Å long) to me. The one before that also seems to be a proteasomal assembly. Hard to say which one but could be a hybrid proteasome (19S one one side of the 20S and PA200 or PA28 on the other side).

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Reposted by Lorenz Grundmann

Kresten Lindorff-Larsen @lindorfflarsen.bsky.social · 4d

A good day to remember John Gurdon’s school report from his biology master at Eton

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Reposted by Lorenz Grundmann

Ulrich Hohmann @hohmannulrich.bsky.social · 19d

How are RNAs sorted for export vs. degradation in the nucleus? In collaboration with @heick.bsky.social’s lab we (@clemensplaschka.bsky.social and @juliusbrennecke.bsky.social labs) discovered a direct mechanistic link between the export and decay machineries: www.biorxiv.org/content/10.1... (1/x)

Molecular basis of polyadenylated RNA fate determination in the nucleus

Eukaryotic genomes generate a plethora of polyadenylated (pA+) RNAs[1][1],[2][2], that are packaged into ribonucleoprotein particles (RNPs). To ensure faithful gene expression, functional pA+ RNPs, in...

www.biorxiv.org

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Reposted by Lorenz Grundmann

Laura Lorenzo Orts @lorenzoorts.bsky.social · 26d

I am excited to announce that I will be moving to IMB Mainz next year! The Winter call for the IPP PhD program is now open; if you are interested in maternal #mRNA regulation and #translation in early vertebrate development, please apply! Deadline: 16 October.

More info: www.imb.de/students-pos...

Laura Lorenzo Orts

IMB Mainz

www.imb.de

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Lorenz Grundmann @lgrundmann.bsky.social · Sep 2

Makes sense, thanks for the explanation 🙂

Looking forward to play around with it once available on EMPIAR. It seems ideal to get a grasp on the processing of (pseudo)symmetric complexes with symmetrie breaking interaction partners.

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Lorenz Grundmann @lgrundmann.bsky.social · Sep 1

I was surprised to see that the classification was that complicated. Did you try refinement and 3D classifications with or without image alignment in C1 before refining in C16?
Either way this is such a cool processing problem resulting in such a cool structure and story.
Much congrats!

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Reposted by Lorenz Grundmann

Dan Grabarczyk @dangrabarczyk.bsky.social · Aug 28

Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...

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Reposted by Lorenz Grundmann

Dan Grabarczyk @dangrabarczyk.bsky.social · Aug 27

Happy to share our work on the structure and function of the unusual E3 ligase ZNFX1 @cp-cell.bsky.social. It uses a nucleic acid-activated transthiolation mechanism, ubiquitinating and clustering RNA to protect cells in an immune response. @clausenlab.bsky.social
www.cell.com/cell/fulltex...

A split-site E3 ligase mechanism enables ZNFX1 to ubiquitinate and cluster single-stranded RNA into ubiquitin-coated nucleoprotein particles

Grabarczyk et al. show the structure and mechanism of a non-canonical ubiquitin ligase, which is activated through nucleic-acid-induced oligomerization and is critical for cell survival during immune ...

www.cell.com

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Reposted by Lorenz Grundmann

Martin Pacesa @martinpacesa.bsky.social · Aug 27

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.

www.nature.com/articles/s41...

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Reposted by Lorenz Grundmann

Sven Klumpe @svenklumpe.bsky.social · Aug 23

If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...

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Reposted by Lorenz Grundmann

Protein Data Bank in Europe (PDBe) @pdbeurope.bsky.social · Aug 20

📄 New paper: 3DEM Structure Map Validation Recommendations

Two new metrics assess model-to-map fitness in cryo-EM:
🔹 Q_relative_all → compares avg Q-score across PDB/EMDB
🔹 Q_relative_resolution → compares within similar resolution

#3DEM #CryoEM #wwPDB

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Reposted by Lorenz Grundmann

Max Planck Institute of Biophysics @mpibp.bsky.social · Aug 18

We’re hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! 🧬❄️ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...

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Reposted by Lorenz Grundmann

Chi-Min Ho @cmholab.bsky.social · Aug 18

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH

A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...

rdcu.be

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Lorenz Grundmann @lgrundmann.bsky.social · Aug 7

Very nice, congratulations "Danger" Daniel 😆

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Reposted by Lorenz Grundmann

DjuranovicLab at Brown University RNA Center @djuranoviclab.bsky.social · Aug 5

Our RAPPL purification of ribosomes and associated material is out. I am really proud of this study in collaboration primary with Pavlovic-Djuranovic lab, as well as Jovanovic and Hashem Labs. This method aims to replace 60 years old purification of ribosomes by sucrose cushions

rdcu.be/ezlhg

A rapid, simple, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies

Nature Communications - Here, the authors present a method to rapidly isolate actively translating ribosomes in a time- and cost-effective manner using poly-lysine. The method is compatible with a...

rdcu.be

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Lorenz Grundmann @lgrundmann.bsky.social · Jul 27

Come join us for the Austrian Cryo-EM Symposium!

Jack Bravo @jpkbravo.bsky.social · Jul 25

Thrilled to announce the nexy Austrian Cryo-EM Symposium, taking place 11th-12th November at @istaresearch.bsky.social, with speakers inc. @grigoriefflab.bsky.social, @oliveiramann.bsky.social, @bjgreber.bsky.social @svenklumpe.bsky.social @sebastianglatt.bsky.social ‪‬https://tinyurl.com/cryo2025

Austrian Cryo-EM Symposium

cryoem-symposium.pages.ist.ac.at

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Reposted by Lorenz Grundmann

Miro Astore @miroastore.bsky.social · Jul 23

That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110

biorxiv.org

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Lorenz Grundmann @lgrundmann.bsky.social · Jul 24

Very cool, congratulations! I was waiting for this 😄
Seems like I will have to compare my favorite methods 🎉

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Reposted by Lorenz Grundmann

Sternberg Lab @sternberglab.bsky.social · May 15

1/10 Today in @science.org in collaboration with
the Liu group we report the development of a laboratory-evolved CRISPR-associated transposase (evoCAST) that supports therapeutically relevant levels of RNA-programmable gene insertion in human cells. drive.google.com/file/d/1I-Ub...

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Reposted by Lorenz Grundmann

Haselbach Lab @haselbachlab.bsky.social · May 15

Congrats to @lgrundmann.bsky.social for winning a poster award at #EMBORNAmeetsproteindecay

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Lorenz Grundmann @lgrundmann.bsky.social · May 15

The 'RNA meets Protein Decay' @embo.org workshop was really a blast. Thanks to everyone for the lively discussions!
I am especially honoured that my poster was awarded with one of the two poster prizes 😇

Pauli Group (posts by Andi Pauli) @pauligroup.bsky.social · May 15

The 2nd edition of the @embo.org workshop 'RNA meets Protein Decay', this time here in Vienna @impvienna.bsky.social, came to an end. Thanks to everyone who came and contributed to the great vibe of sharing science, discussing ideas and connecting with each other!

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Reposted by Lorenz Grundmann

Pauli Group (posts by Andi Pauli) @pauligroup.bsky.social · May 15

The 2nd edition of the @embo.org workshop 'RNA meets Protein Decay', this time here in Vienna @impvienna.bsky.social, came to an end. Thanks to everyone who came and contributed to the great vibe of sharing science, discussing ideas and connecting with each other!

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Reposted by Lorenz Grundmann

Haselbach Lab @haselbachlab.bsky.social · Apr 7

We are looking for PhD students using time resolved cryoEM or cryoET on topics of the ubiquitin proteasome system, fertility and immunity. However, there is freedom to shape projects in own ways.

IMP @impvienna.bsky.social · Apr 7

Passionate about structural biology and #biochemistry? Join David Haselbach’s group through the Vienna BioCenter #PhD Program!

⏳ Deadline: April 15 🚨, apply now: training.vbc.ac.at/phd-program/

📽️Watch David's 'Scientist Snapshot': youtube.com/watch?v=DXTO...

@haselbachlab.bsky.social

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Lorenz Grundmann @lgrundmann.bsky.social · Apr 9

Amazing structures and more by my colleague Sascha Amann explaining how the 26S proteasome interacts with K48 and K48-K11 branched ubiquitin chains. I think that this is a crucial puzzle piece in understanding substrate engagement by the proteasome.
It was a pleasure seeing this project unfold 🚀

Haselbach Lab @haselbachlab.bsky.social · Apr 9

New Preprint: We have solved the structure of the proteasome in complex with two different ubiquitin chains: www.biorxiv.org/content/10.1... This work is spearheaded by PhD Student Sascha Amann
Let me walk you through it 🧵 1/9

Structural basis for the ubiquitin chain recognition of the human 26S proteasome

Proteasomal degradation is a fundamental process for all eukaryotic life. A protein destined for degradation is first tagged with a polyubiquitin chain, which is selected by the proteasome. Different ...

www.biorxiv.org

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