Luis G. Palma
LightNews LightNews
banner
luigalpal.bsky.social
Luis G. Palma
@luigalpal.bsky.social
110 followers 170 following 40 posts
Stem Cell Biologist in Nuñez lab, in the Molecular and Cell Biology Dept. of UC Berkeley. Interested in #StemCellFate, #Chromatin, #Epigenetics and #CRISPRtools. Previously, PhD at Hospital Del Mar and Carreras Leukemia Research Institutes.
Posts Replies Media Videos
luigalpal.bsky.social
Outstanding paper describing how SVAs are regulated,what's their function in gene regulation and its impact in cell fate

Composite transposons with bivalent histone marks function as RNA-dependent enhancers in cell fate regulation | Cell

#TEs #CellFate #HSCs #Aging

www.cell.com/cell/fulltex...
Composite transposons with bivalent histone marks function as RNA-dependent enhancers in cell fate regulation
This study reveals that composite transposons with bivalent histone marks can serve as RNA-dependent enhancers, controlling key developmental and aging-related gene programs.
www.cell.com
August 7, 2025 at 9:38 PM
Reposted by Luis G. Palma
stephenturner.us
Human whole epigenome modelling for clinical applications with Pleiades www.biorxiv.org/content/10.1... 🧬🖥️🧪
August 7, 2025 at 6:30 PM
luigalpal.bsky.social
Thanks everyone involved in the work. Special thanks also to our amazing team of collaborators: @mercedesbarrero.bsky.social, Payer lab and Menendez lab
luigalpal.bsky.social Luis G. Palma @luigalpal.bsky.social · Jul 18
Thrilled to share with you the latest work from my PhD in Bigas lab at @researchmar.bsky.social @carrerasijc.bsky.social published in @bloodjournal.bsky.social

We’ve programmed blood stem cells from PSCs using CRISPRa screen and found a new “bloody” cocktail

#CRISPR #HSCs #StemCells #CellFate
kwnsfk27.r.eu-west-1.awstrack.me
July 18, 2025 at 6:30 PM
luigalpal.bsky.social
Thrilled to share with you the latest work from my PhD in Bigas lab at @researchmar.bsky.social @carrerasijc.bsky.social published in @bloodjournal.bsky.social

We’ve programmed blood stem cells from PSCs using CRISPRa screen and found a new “bloody” cocktail

#CRISPR #HSCs #StemCells #CellFate
kwnsfk27.r.eu-west-1.awstrack.me
July 18, 2025 at 6:28 PM
luigalpal.bsky.social
Very happy to have our latest work on how IκBα regulates stemness independently from NF-κB. It has been a terrific collaborative work led by Lluís Espinosa and Daniel Alvarez-Villanueva @researchmar.bsky.social

#nfkb #ikb #inflammation #stemcells

www.cell.com/cell-reports...
Separation-of-function mutants reveal the NF-κB-independent involvement of IκBα in the regulation of intestinal stemness
Daniel Álvarez-Villanueva et al. dissect distinct roles of the NF-κB inhibitor IκBα by generating separation-of-function mutants. Their findings reveal that chromatin-bound IκBα, independent of NF-κB ...
www.cell.com
July 12, 2025 at 12:20 AM
luigalpal.bsky.social
H3K4 methylation instructs blood specification in vivo

Bivalent chromatin instructs lineage specification during hematopoiesis | Cell

#Hematopoiesis #CellFate #Chromatin

www.cell.com/cell/abstrac...
Bivalent chromatin instructs lineage specification during hematopoiesis
Yagi et al. show that, unexpectedly, H3K4 methylation is dispensable for hematopoietic stem cell maintenance but essential for progenitor cell maturation into all blood cell types by opposing H3K27 me...
www.cell.com
June 17, 2025 at 4:38 PM
Reposted by Luis G. Palma
andersshansen.bsky.social
(1/n) Thread @matteomazzocca.bsky.social @domenicnarducci.bsky.social Simon Grosse-Holz @jessematthias.bsky.social preprint

Q: how does chromatin move?

Using MINFLUX, SPT & SRLCI, we track chromatin dynamics across 7 orders of magnitude in time to provide answers www.biorxiv.org/content/10.1...
May 15, 2025 at 12:37 PM
luigalpal.bsky.social
Nice review about alternative roles of nucleolus in genome organization in stem cells

Emerging roles for the nucleolus in development and stem cells | Development

#Review #Heterochromatin #GenomeOrganization #StemCells

journals.biologists.com/dev/article/...
Emerging roles for the nucleolus in development and stem cells
Summary: This Review explores roles of the nucleolus in mammalian development, with a particular focus on genome organisation and chromatin regulation.
journals.biologists.com
May 15, 2025 at 12:42 PM
Reposted by Luis G. Palma
events.embl.org
Lively conversations, shared laughs, and engaging networking during the breaks at #EMBLChromatin 🧬💬🤝
May 14, 2025 at 12:51 PM
Reposted by Luis G. Palma
deniz-lab.bsky.social
I am excited to share our lab's first review out with in Blood Cancer Journal. We discuss the impact of transposable elements on genome regulation and variation in both normal
haematopoietic processes and haematological cancers. www.nature.com/articles/s41...
Transposable elements as genome regulators in normal and malignant haematopoiesis - Blood Cancer Journal
Blood Cancer Journal - Transposable elements as genome regulators in normal and malignant haematopoiesis
www.nature.com
May 6, 2025 at 4:31 PM
luigalpal.bsky.social
Review about principles of stem cell biology and cellular reprogramming approaches and their use for current and future therapeutic purposes

Stem cells as role models for reprogramming and repair | Science

#Review #StemCells #Reprogramming #RegenerativeMedicine

www.science.org/doi/10.1126/...
Stem cells as role models for reprogramming and repair
Stem cells are a promising source for cellular therapies across many diseases and tissues. Their inherent ability to differentiate into other cell types has been the focus of investigation over decade...
www.science.org
May 1, 2025 at 10:43 PM
luigalpal.bsky.social
Maternal H3K27me3 inheritance over de novo methylation shapes chromatin architecture

The establishment of nuclear organization in mouse embryos is orchestrated by multiple epigenetic pathways | Cell

#3DGenome #Chromatin #Development

www.cell.com/cell/fulltex...
The establishment of nuclear organization in mouse embryos is orchestrated by multiple epigenetic pathways
In vivo screening in mouse embryos provides insights into the functional interplay between chromatin and the nuclear envelope in the establishment of lamina-associated domains, the earliest feature of...
www.cell.com
April 25, 2025 at 4:34 PM
luigalpal.bsky.social
Transcription factor networks disproportionately enrich for heritability of blood cell phenotypes | Science

#TranscriptionFactors #GeneRegulation #Hematopoiesis

www.science.org/doi/10.1126/...
Transcription factor networks disproportionately enrich for heritability of blood cell phenotypes
Most phenotype-associated genetic variants map to noncoding regulatory regions of the human genome, but their mechanisms remain elusive in most cases. We developed a highly efficient strategy, Perturb...
www.science.org
April 4, 2025 at 5:28 AM
Reposted by Luis G. Palma
peterlewislab.bsky.social
The HuSH complex silences retrotransposable elements. We identify HuSH2, centered on TASOR2, targeting KRAB-ZNFs and interferon genes, balancing retrotransposon silencing and immune regulation. www.nature.com/articles/s41...
November 3, 2024 at 3:20 PM
luigalpal.bsky.social
p300 acetylates METTL3 specifically on H3K27ac chromatin to promote transcription

Spatial control of m6A deposition on enhancer and promoter RNAs through co-acetylation of METTL3 and H3K27 on chromatin | Molecular Cell

#m6a #chromatin #transcription #mettl3

www.sciencedirect.com/science/arti...
Spatial control of m6A deposition on enhancer and promoter RNAs through co-acetylation of METTL3 and H3K27 on chromatin
Interaction between the N6-methyladenosine (m6A) methyltransferase METTL3 and METTL14 is critical for METTL3 to deposit m6A on various types of RNAs. …
www.sciencedirect.com
March 17, 2025 at 4:24 PM
luigalpal.bsky.social
The molecular basis of heterochromatin assembly and epigenetic inheritance | Molecular Cell

#Heterochromatin #EpigeneticInheritance #Review

www.sciencedirect.com/science/arti...
The molecular basis of heterochromatin assembly and epigenetic inheritance
Heterochromatin plays a fundamental role in gene regulation, genome integrity, and silencing of repetitive DNA elements. Histone modifications are ess…
www.sciencedirect.com
March 11, 2025 at 10:07 PM
Reposted by Luis G. Palma
jamesbriscoe.bsky.social
Our latest: uncovering hierarchical design rules for cis-regulatory elements with a high-throughput screen

CRE activity follows multiplicative modular rules, tuned by spacing & positioning

Enables engineering synthetic CREs for predictable tissue patterning

www.biorxiv.org/content/10.1...
Predictable Engineering of Signal-Dependent Cis-Regulatory Elements
www.biorxiv.org
March 10, 2025 at 4:19 AM
luigalpal.bsky.social
Changes in promoter histone acetylation and gene bursting are uncoupled

Identification of molecular determinants of gene-specific bursting patterns by high-throughput imaging screens | Molecular Cell

#GeneRegulation #Transcription

www.cell.com/molecular-ce...
Identification of molecular determinants of gene-specific bursting patterns by high-throughput imaging screens
Sood et al. used high-throughput screening to identify regulators of gene bursting and find both gene- and cell-type-specific effectors. Acetylation alters burst size in a gene-specific manner but is ...
www.cell.com
March 7, 2025 at 5:08 PM
luigalpal.bsky.social
Transposon–host arms race: a saga of genome evolution | Trends in Genetics

#review #transposon #HUSH #KRAB

www.cell.com/trends/genet...
Transposon–host arms race: a saga of genome evolution
Once considered ‘junk DNA,’ transposons or transposable elements (TEs) are now recognized as key drivers of genome evolution, contributing to genetic diversity, gene regulation, and species diversific...
www.cell.com
February 25, 2025 at 5:22 PM
Reposted by Luis G. Palma
grothlab.bsky.social
𝐇𝐨𝐰 𝐝𝐨 𝐜𝐞𝐥𝐥𝐬 𝐫𝐞𝐦𝐞𝐦𝐛𝐞𝐫 𝐰𝐡𝐨 𝐭𝐡𝐞𝐲 𝐚𝐫𝐞 𝐚𝐟𝐭𝐞𝐫 𝐃𝐍𝐀 𝐫𝐞𝐩𝐥𝐢𝐜𝐚𝐭𝐢𝐨𝐧? Our new study “Disabling leading and lagging strand histone transmission results in parental histones loss and reduced cell plasticity and viability” is out in 𝘚𝘤𝘪𝘦𝘯𝘤𝘦 𝘈𝘥𝘷𝘢𝘯𝘤𝘦𝘴. Led by @lleonie.bsky.social @biranalva.bsky.social 🧵 More below👇
Disabling leading and lagging strand histone transmission results in parental histones loss and reduced cell plasticity and viability
Losing parental histones during DNA replication fork passage challenges differentiation competence and cell viability.
tinyurl.com
February 19, 2025 at 7:35 PM
luigalpal.bsky.social
Histone modifications occur independently of transcription

RNA polymerase II coordinates histone deacetylation at active promoters | Science Advances

#chromatin #transcription #generegulation

www.science.org/doi/10.1126/...
RNA polymerase II coordinates histone deacetylation at active promoters
Transcription initiation limits histone acetylation and H2AZ incorporation at promoters.
www.science.org
February 13, 2025 at 5:49 PM
luigalpal.bsky.social
Sequencing technologies to measure translation in single cells | Nature Reviews Molecular Cell Biology

#Review #Sequencing #Translation #SingleCell

www.nature.com/articles/s41...
Sequencing technologies to measure translation in single cells - Nature Reviews Molecular Cell Biology
Recent sequencing-based methods, including novel ribosome profiling, ribosome affinity purification and spatial translation methods, have enabled the measurement of translation at single-cell resoluti...
www.nature.com
January 21, 2025 at 5:51 PM
luigalpal.bsky.social
Uncovering the whole genome silencers of human cells via Ss-STARR-seq | Nature Communications

#Chromatin #GeneRegulation #Enhancers

www.nature.com/articles/s41...
Uncovering the whole genome silencers of human cells via Ss-STARR-seq - Nature Communications
Silencers play a critical role in gene transcription regulation. Here, the authors developed the Ss-STARR-seq technique to identify tens of thousands of silencers in human cells. These silencers posse...
www.nature.com
January 21, 2025 at 5:48 PM
luigalpal.bsky.social
Linking RNA modifications to DNMT1 function on gene bodies

Fine-tuning of gene expression through the Mettl3-Mettl14-Dnmt1 axis controls ESC differentiation | Cell

#Epigenetics #DNAme #Development #DNMT1 #RNA

www.cell.com/cell/abstrac...
Fine-tuning of gene expression through the Mettl3-Mettl14-Dnmt1 axis controls ESC differentiation
Beyond its role as RNA m6A writer, METTL3-METTL14 can mediate 5mC deposition on DNA by recruiting the DNA methyltransferase DNMT1 to chromatin. The two marks co-orchestrate the expression of key genes...
www.cell.com
January 18, 2025 at 1:36 AM
luigalpal.bsky.social
Promoters have strong orientation-bias, but not the enhancers

Massively parallel characterization of transcriptional regulatory elements | Nature

#transcription #generegulation #chromatin

www.nature.com/articles/s41...
Massively parallel characterization of transcriptional regulatory elements - Nature
Lentivirus-based reporter assays for 680,000 regulatory sequences from three cell lines coupled to machine-learning models lead to insights into the grammar of cis-regulatory elements.
www.nature.com
January 16, 2025 at 1:36 AM