Nandan Gokhale
@nandangokhale.bsky.social
Interested in how RNA regulates and is regulated by infection and immunity. Assistant Professor at CCHMC Immunobiology: www.gokhalelab.com
Pinned
Nandan Gokhale
@nandangokhale.bsky.social
· Dec 19
Cellular RNA interacts with MAVS to promote antiviral signaling
Antiviral signaling downstream of RIG-I–like receptors (RLRs) proceeds through a multi-protein complex organized around the adaptor protein mitochondrial antiviral signaling protein (MAVS). Protein co...
www.science.org
Our work on cellular RNA interactions with MAVS that promote antiviral signaling is finally live. So grateful to our indispensable collaborators as well as reviewers who helped to strengthen our work. #RNA #innateimmunity #virology
@ramlabuw.bsky.social
www.science.org/doi/10.1126/...
@ramlabuw.bsky.social
www.science.org/doi/10.1126/...
Reposted by Nandan Gokhale
Check out our new publication, a collaboration with Giovanni Luchetti and Vishva Dixit @ Genentech.
We show that the E.coli effector NleL inhibits intestinal epithelial cell extrusion after #inflammasome activation via degrading the kinases ROCK1 and 2!!
www.nature.com/articles/s41...
Explainer 1/8
We show that the E.coli effector NleL inhibits intestinal epithelial cell extrusion after #inflammasome activation via degrading the kinases ROCK1 and 2!!
www.nature.com/articles/s41...
Explainer 1/8
Enteropathogenic bacteria evade ROCK-driven epithelial cell extrusion - Nature
The bacterial ubiquitin ligase NleL evades host defence mechanisms both by inhibiting pyroptosis and by preventing infected intestinal epithelial cells from being extruded into the lumen and expelled ...
www.nature.com
October 22, 2025 at 3:47 PM
Check out our new publication, a collaboration with Giovanni Luchetti and Vishva Dixit @ Genentech.
We show that the E.coli effector NleL inhibits intestinal epithelial cell extrusion after #inflammasome activation via degrading the kinases ROCK1 and 2!!
www.nature.com/articles/s41...
Explainer 1/8
We show that the E.coli effector NleL inhibits intestinal epithelial cell extrusion after #inflammasome activation via degrading the kinases ROCK1 and 2!!
www.nature.com/articles/s41...
Explainer 1/8
Wonderful opportunity. Go join Emmanuelle’s lab!
Excitingly, we are now hiring at all levels!! Please reach out if you are interested in RNA viruses, viral replication, innate immunity, RNA imaging, or are just excited about having fun doing cool science! Information about specific positions here www.genoyerlab.com/join
Join — Genoyer Lab
www.genoyerlab.com
October 4, 2025 at 12:36 PM
Wonderful opportunity. Go join Emmanuelle’s lab!
Reposted by Nandan Gokhale
I'm thrilled to announce I'll be joining the Department of Molecular Genetics & Microbiology at the University of Florida in January 2026! My lab will study RNA virus-host interactions with a particular focus on the spatial regulation of viral replication and innate immune activation. genoyerlab.com
Genoyer Lab
genoyerlab.com
October 3, 2025 at 6:10 PM
I'm thrilled to announce I'll be joining the Department of Molecular Genetics & Microbiology at the University of Florida in January 2026! My lab will study RNA virus-host interactions with a particular focus on the spatial regulation of viral replication and innate immune activation. genoyerlab.com
Reposted by Nandan Gokhale
Last Saturday morning ☕ saved for this gem:
www.science.org/doi/10.1126/...
CRISPR screening reveals TRIM25 as a pH-sensitive sensor that specifically targets foreign RNAs entering through endosomes. N1-methylpseudouridine helps repel TRIM25 binding & enhances protein production.
www.science.org/doi/10.1126/...
CRISPR screening reveals TRIM25 as a pH-sensitive sensor that specifically targets foreign RNAs entering through endosomes. N1-methylpseudouridine helps repel TRIM25 binding & enhances protein production.
Exogenous RNA surveillance by proton-sensing TRIM25
Exogenous messenger RNAs (mRNAs) require cellular machinery for delivery and translation but also encounter inhibitory factors. To investigate their regulation, we performed genome-wide CRISPR screens...
www.science.org
April 5, 2025 at 2:10 PM
Last Saturday morning ☕ saved for this gem:
www.science.org/doi/10.1126/...
CRISPR screening reveals TRIM25 as a pH-sensitive sensor that specifically targets foreign RNAs entering through endosomes. N1-methylpseudouridine helps repel TRIM25 binding & enhances protein production.
www.science.org/doi/10.1126/...
CRISPR screening reveals TRIM25 as a pH-sensitive sensor that specifically targets foreign RNAs entering through endosomes. N1-methylpseudouridine helps repel TRIM25 binding & enhances protein production.
Congrats to Julian and others from @ramlabuw.bsky.social on this cool work that finds that the loss of the RNA-binding protein CELF2 leads to accumulation of endogenous RIG-I ligands! #RNA #innateimmunity #IFNs
CELF2 suppresses immunostimulatory endogenous RNAs that activate the RIG-I-MAVS pathway in macrophages, preventing type I IFN-signature. Congrats to Julian Smith, Olivia Kern, and all the co-authors for their hard work! #innateimmunity #dsRNA #IFN
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
April 1, 2025 at 11:27 PM
Congrats to Julian and others from @ramlabuw.bsky.social on this cool work that finds that the loss of the RNA-binding protein CELF2 leads to accumulation of endogenous RIG-I ligands! #RNA #innateimmunity #IFNs
Really cool work from @thehornerlab.bsky.social and colleagues highlighting a role for the UFMylation post-translational modification system in flavivirus viral particle production!
UFMylation promotes orthoflavivirus infectious particle production https://www.biorxiv.org/content/10.1101/2025.01.09.632082v1
January 11, 2025 at 5:35 AM
Really cool work from @thehornerlab.bsky.social and colleagues highlighting a role for the UFMylation post-translational modification system in flavivirus viral particle production!
Reposted by Nandan Gokhale
Reposting my offer for 1-1 mentoring for anyone… email me and we'll find a time! Especially happy to talk with those from diverse backgrounds and non-traditional career trajectories.
January 2, 2025 at 4:02 PM
Reposting my offer for 1-1 mentoring for anyone… email me and we'll find a time! Especially happy to talk with those from diverse backgrounds and non-traditional career trajectories.
Reposted by Nandan Gokhale
We have an open faculty search for TWO positions in microbiology and virology at the University of Illinois! We have world class core facilities and a fantastic collaborative group of young faculty...you could be next!
Please re-post/skeet!
Please re-post/skeet!
September 9, 2024 at 8:36 PM
We have an open faculty search for TWO positions in microbiology and virology at the University of Illinois! We have world class core facilities and a fantastic collaborative group of young faculty...you could be next!
Please re-post/skeet!
Please re-post/skeet!
I am so excited to join the faculty in the Division of Immunobiology at Cincinnati Children's Hospital Medical Center in March 2025. My lab will study how RNA biology regulates and is regulated by infection and immunity.
We'll be hiring at all levels in 2025 - stay tuned!
www.gokhalelab.com
We'll be hiring at all levels in 2025 - stay tuned!
www.gokhalelab.com
Excited that @nandangokhale.bsky.social plans to join the faculty at Cincinnati Children's in March! Check out the UW story about the study: www.eurekalert.org/news-release...
December 20, 2024 at 1:48 AM
I am so excited to join the faculty in the Division of Immunobiology at Cincinnati Children's Hospital Medical Center in March 2025. My lab will study how RNA biology regulates and is regulated by infection and immunity.
We'll be hiring at all levels in 2025 - stay tuned!
www.gokhalelab.com
We'll be hiring at all levels in 2025 - stay tuned!
www.gokhalelab.com
Reposted by Nandan Gokhale
Cellular RNAs directly regulate the activity of an antiviral immune signaling complex
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
@science.org @nandangokhale.bsky.social @ramlabuw.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
@science.org @nandangokhale.bsky.social @ramlabuw.bsky.social
December 19, 2024 at 8:03 PM
Cellular RNAs directly regulate the activity of an antiviral immune signaling complex
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
@science.org @nandangokhale.bsky.social @ramlabuw.bsky.social
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
@science.org @nandangokhale.bsky.social @ramlabuw.bsky.social
Reposted by Nandan Gokhale
This Perspective by Sonja Best and Adam Hage puts tuning the MAVS signalosome by cellular mRNAs into context.
buff.ly/4fxVMcx
#Immunology @science.org
buff.ly/4fxVMcx
#Immunology @science.org
December 19, 2024 at 8:09 PM
This Perspective by Sonja Best and Adam Hage puts tuning the MAVS signalosome by cellular mRNAs into context.
buff.ly/4fxVMcx
#Immunology @science.org
buff.ly/4fxVMcx
#Immunology @science.org
Reposted by Nandan Gokhale
#Immunology out now @science.org
An unstructured region within MAVS interacts with cellular, rather than viral, RNA. This alters how MAVS interacts with other proteins and influences antiviral responses.
https://buff.ly/4grnf18
By @nandangokhale.bsky.social with @ramlabuw.bsky.social et al.
An unstructured region within MAVS interacts with cellular, rather than viral, RNA. This alters how MAVS interacts with other proteins and influences antiviral responses.
https://buff.ly/4grnf18
By @nandangokhale.bsky.social with @ramlabuw.bsky.social et al.
December 19, 2024 at 8:05 PM
#Immunology out now @science.org
An unstructured region within MAVS interacts with cellular, rather than viral, RNA. This alters how MAVS interacts with other proteins and influences antiviral responses.
https://buff.ly/4grnf18
By @nandangokhale.bsky.social with @ramlabuw.bsky.social et al.
An unstructured region within MAVS interacts with cellular, rather than viral, RNA. This alters how MAVS interacts with other proteins and influences antiviral responses.
https://buff.ly/4grnf18
By @nandangokhale.bsky.social with @ramlabuw.bsky.social et al.
Our work on cellular RNA interactions with MAVS that promote antiviral signaling is finally live. So grateful to our indispensable collaborators as well as reviewers who helped to strengthen our work. #RNA #innateimmunity #virology
@ramlabuw.bsky.social
www.science.org/doi/10.1126/...
@ramlabuw.bsky.social
www.science.org/doi/10.1126/...
Cellular RNA interacts with MAVS to promote antiviral signaling
Antiviral signaling downstream of RIG-I–like receptors (RLRs) proceeds through a multi-protein complex organized around the adaptor protein mitochondrial antiviral signaling protein (MAVS). Protein co...
www.science.org
December 19, 2024 at 8:13 PM
Our work on cellular RNA interactions with MAVS that promote antiviral signaling is finally live. So grateful to our indispensable collaborators as well as reviewers who helped to strengthen our work. #RNA #innateimmunity #virology
@ramlabuw.bsky.social
www.science.org/doi/10.1126/...
@ramlabuw.bsky.social
www.science.org/doi/10.1126/...
Reposted by Nandan Gokhale
Time for our first post here 🥳 Our latest paper is online 🙌
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo
The acute skin reaction to sunburn, encompassing keratinocyte cell death, inflammation,
and epidermal thickening, has traditionally been ascribed to DNA damage responses.
Using a mouse model deficient...
www.cell.com
November 26, 2024 at 10:02 AM
Time for our first post here 🥳 Our latest paper is online 🙌
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
Rachel Van Gelder, a recently minted PhD from the lab, shows IL-2 splices IFNG mRNA introns, increasing protein levels via NF-κB signaling. She uncovers a novel role for cytokine-induced splicing in immunity! 🧬 #RNA #Immunology #Research
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
Interleukin-2-mediated NF-κB-dependent mRNA splicing modulates interferon gamma protein production | EMBO reports
imageimageIL-2 stimulation post-transcriptionally enhances splicing of IFNG mRNA in NK cells. Further, NF-κB signaling downstream of the IL-2 receptor is required
for IFNG mRNA processing. IL-12 and ...
www.embopress.org
November 22, 2024 at 6:32 PM
Reposted by Nandan Gokhale
CHMC Division of Immunobiology is hiring Assistant Professors. You can have a lab next to mine if you like.
November 20, 2024 at 7:33 PM
CHMC Division of Immunobiology is hiring Assistant Professors. You can have a lab next to mine if you like.
Reposted by Nandan Gokhale
#omap4lyfe. We've had a great experience using this protocol-- five stars, highly recommend. @pspeck-enthusiast.bsky.social
It's my great pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope will transform the study of nuclear architecture.
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
November 21, 2024 at 4:46 PM
#omap4lyfe. We've had a great experience using this protocol-- five stars, highly recommend. @pspeck-enthusiast.bsky.social
O-MAP is a gift that will keep on giving!
It's my great pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope will transform the study of nuclear architecture.
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
November 21, 2024 at 1:49 AM
O-MAP is a gift that will keep on giving!
Reposted by Nandan Gokhale
It's my great pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope will transform the study of nuclear architecture.
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
November 21, 2024 at 1:27 AM
It's my great pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope will transform the study of nuclear architecture.
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)
Reposted by Nandan Gokhale
Hi everyone! New to Bluesky, but happy to share the final chapter of my PhD @uconnhealth.bsky.social out today in Nature Immunology! Here, we uncovered a noncanonical function of the glucose transporter GLUT4 in regulating RLR signaling in muscle and adipocytes
www.nature.com/articles/s41...
www.nature.com/articles/s41...
UBXN9 governs GLUT4-mediated spatial confinement of RIG-I-like receptors and signaling - Nature Immunology
Wang and colleagues show that in skeletal muscle cells and cardiomyocytes, the glucose transporter GLUT4 is a negative regulator of RIG-I-like receptor signaling during viral infection by redistributi...
www.nature.com
November 20, 2024 at 7:28 PM
Hi everyone! New to Bluesky, but happy to share the final chapter of my PhD @uconnhealth.bsky.social out today in Nature Immunology! Here, we uncovered a noncanonical function of the glucose transporter GLUT4 in regulating RLR signaling in muscle and adipocytes
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Nandan Gokhale
Victim of its success the Virology and Virus-Host interaction starter pack got packed (150 users) so I created #2 ! Reach out if you want to be included ! go.bsky.app/6bSDmrW
November 18, 2024 at 11:50 AM
Victim of its success the Virology and Virus-Host interaction starter pack got packed (150 users) so I created #2 ! Reach out if you want to be included ! go.bsky.app/6bSDmrW
Reposted by Nandan Gokhale
A starter pack for all virologists out there on the blue side ! Might be missing some people but it’s a good start ! go.bsky.app/DmphBCT
November 17, 2024 at 10:05 AM
A starter pack for all virologists out there on the blue side ! Might be missing some people but it’s a good start ! go.bsky.app/DmphBCT
Reposted by Nandan Gokhale
Excited to see many in the bacterial pathogenesis community joining here in the last few weeks, so here's a Starter Pack to help make connections. Reply/DM to be added to this or future Packs. #Microsky go.bsky.app/VH64BaY
November 17, 2024 at 1:46 AM
Excited to see many in the bacterial pathogenesis community joining here in the last few weeks, so here's a Starter Pack to help make connections. Reply/DM to be added to this or future Packs. #Microsky go.bsky.app/VH64BaY
Reposted by Nandan Gokhale
I’ve made a Starter Pack of #innateimmunity and #inflammation researchers to follow! Please contact me to get added or to suggest others to add. We are just getting started 😎
#immunosky
#academicchatter
go.bsky.app/QqJ9AgB
#immunosky
#academicchatter
go.bsky.app/QqJ9AgB
November 16, 2024 at 10:10 PM
I’ve made a Starter Pack of #innateimmunity and #inflammation researchers to follow! Please contact me to get added or to suggest others to add. We are just getting started 😎
#immunosky
#academicchatter
go.bsky.app/QqJ9AgB
#immunosky
#academicchatter
go.bsky.app/QqJ9AgB
Shouldn’t it spontaneously assemble and fuse with other starter packs?
Is there a phase separation/condensate starter pack?
November 15, 2024 at 3:13 AM
Shouldn’t it spontaneously assemble and fuse with other starter packs?