Felipe Opazo
@opazo.bsky.social
PI at the Uni Medical Center Göttingen (UMG), Founder and CTO of NanoTag Biotechnologies. Using nanobodies for super-resolution microscopy in neurosciences. Opinions are my own.
Reposted by Felipe Opazo
Delighted to share our latest preprint "Filopodia-mediated trans-endocytosis"
www.biorxiv.org/content/10.6...
Work driven by many people including Hanna, Marcela, Sujan, Marie-Catherine, Anna and Monika. Fantastic collaboration with Johanna Englund, Emilia Peuhu, and Eija Jokitalo
www.biorxiv.org/content/10.6...
Work driven by many people including Hanna, Marcela, Sujan, Marie-Catherine, Anna and Monika. Fantastic collaboration with Johanna Englund, Emilia Peuhu, and Eija Jokitalo
February 11, 2026 at 2:20 PM
Delighted to share our latest preprint "Filopodia-mediated trans-endocytosis"
www.biorxiv.org/content/10.6...
Work driven by many people including Hanna, Marcela, Sujan, Marie-Catherine, Anna and Monika. Fantastic collaboration with Johanna Englund, Emilia Peuhu, and Eija Jokitalo
www.biorxiv.org/content/10.6...
Work driven by many people including Hanna, Marcela, Sujan, Marie-Catherine, Anna and Monika. Fantastic collaboration with Johanna Englund, Emilia Peuhu, and Eija Jokitalo
Reposted by Felipe Opazo
🧪🧬 New preprint!
What if the same biosensor could reveal how cysteine is regulated across life domains?
We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells.
www.biorxiv.org/content/10.6...
🧵A thread ⬇️
What if the same biosensor could reveal how cysteine is regulated across life domains?
We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells.
www.biorxiv.org/content/10.6...
🧵A thread ⬇️
February 9, 2026 at 11:56 AM
🧪🧬 New preprint!
What if the same biosensor could reveal how cysteine is regulated across life domains?
We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells.
www.biorxiv.org/content/10.6...
🧵A thread ⬇️
What if the same biosensor could reveal how cysteine is regulated across life domains?
We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells.
www.biorxiv.org/content/10.6...
🧵A thread ⬇️
Reposted by Felipe Opazo
Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render)
@zeiss-microscopy.bsky.social
@zeiss-microscopy.bsky.social
December 24, 2025 at 1:16 PM
Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render)
@zeiss-microscopy.bsky.social
@zeiss-microscopy.bsky.social
Reposted by Felipe Opazo
We also tried it recently and indeed, it blinks nicely without any smelly buffers or extra stuff added. Makes SMLM sample preparation a breeze. I hope soon new additional colors will be discovered, apart from the far red.
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
December 22, 2025 at 11:44 AM
We also tried it recently and indeed, it blinks nicely without any smelly buffers or extra stuff added. Makes SMLM sample preparation a breeze. I hope soon new additional colors will be discovered, apart from the far red.
Reposted by Felipe Opazo
🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners.
Here’s to what’s next 🥂
Here’s to what’s next 🥂
December 22, 2025 at 10:36 AM
🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners.
Here’s to what’s next 🥂
Here’s to what’s next 🥂
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
December 22, 2025 at 10:45 AM
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Happy to tell that I got my tenured professorship at the University Medical Center in Göttingen 🇩🇪!
Grateful for my students, mentors, international collaborators, the @nanotag.bsky.social team, and my family (dog included 🐾). Motivated to keep pushing science, innovation, startups, and education 🌍✨
Grateful for my students, mentors, international collaborators, the @nanotag.bsky.social team, and my family (dog included 🐾). Motivated to keep pushing science, innovation, startups, and education 🌍✨
October 17, 2025 at 3:06 PM
Happy to tell that I got my tenured professorship at the University Medical Center in Göttingen 🇩🇪!
Grateful for my students, mentors, international collaborators, the @nanotag.bsky.social team, and my family (dog included 🐾). Motivated to keep pushing science, innovation, startups, and education 🌍✨
Grateful for my students, mentors, international collaborators, the @nanotag.bsky.social team, and my family (dog included 🐾). Motivated to keep pushing science, innovation, startups, and education 🌍✨
Reposted by Felipe Opazo
#NanoTag joins the Life Science Ecosystem at Nordic Life Science Days #NLSDays2025 in Gothenburg!🇸🇪 Meet our CEO Hansjörg Götzke at the Booth B03:46 (German Pavilion), and discover our sdAb platforms🦙, protein production services, and research tools🔬advancing #LifeScience & #Biomedicine.
October 7, 2025 at 8:17 AM
#NanoTag joins the Life Science Ecosystem at Nordic Life Science Days #NLSDays2025 in Gothenburg!🇸🇪 Meet our CEO Hansjörg Götzke at the Booth B03:46 (German Pavilion), and discover our sdAb platforms🦙, protein production services, and research tools🔬advancing #LifeScience & #Biomedicine.
Fantastic talk of @ewerslab.bsky.social with ExM data on Septins and Unroof Cells in #GEF25!
September 28, 2025 at 7:35 AM
Fantastic talk of @ewerslab.bsky.social with ExM data on Septins and Unroof Cells in #GEF25!
Reposted by Felipe Opazo
🦙 NanoTag at #SingleDomainAntibodies Conference 2025!
Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics.
Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.
Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics.
Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.
September 25, 2025 at 9:22 AM
🦙 NanoTag at #SingleDomainAntibodies Conference 2025!
Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics.
Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.
Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics.
Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.
Reposted by Felipe Opazo
#ExM turned 10 this year - just like #NanoTag! 🎉
Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging.
See you in Göttingen!
#GEF25
Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging.
See you in Göttingen!
#GEF25
September 17, 2025 at 8:33 AM
#ExM turned 10 this year - just like #NanoTag! 🎉
Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging.
See you in Göttingen!
#GEF25
Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging.
See you in Göttingen!
#GEF25
Reposted by Felipe Opazo
Prefusion gB of HSV bound with a neutralizing nanobody www.nature.com/articles/s41...
A nanobody specific to prefusion glycoprotein B neutralizes HSV-1 and HSV-2 - Nature
A neutralizing nanobody specific to the prefusion conformation of herpes simplex virus glycoprotein B has cross-species activity and offers insights into virus neutralization, possible immunogens and ...
www.nature.com
September 4, 2025 at 5:51 AM
Prefusion gB of HSV bound with a neutralizing nanobody www.nature.com/articles/s41...
Reposted by Felipe Opazo
🔬Wrapping up #SMLMS2025 @smlm-symposium.bsky.social in Bonn! Thanks to everyone who stopped by our booth!
It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy.
See you next year! 🦙
It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy.
See you next year! 🦙
August 29, 2025 at 11:33 AM
🔬Wrapping up #SMLMS2025 @smlm-symposium.bsky.social in Bonn! Thanks to everyone who stopped by our booth!
It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy.
See you next year! 🦙
It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy.
See you next year! 🦙
Reposted by Felipe Opazo
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 21, 2025 at 5:19 AM
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
🧬 New paper out! We explored why #antibodies have kept their 2 binding sites. Turns out, this “divalence” is crucial for B-cell receptors to sense antigen clustering and trigger immune responses, especially for weak or low-density antigens.
Check full text: doi.org/10.1038/s414...
Check full text: doi.org/10.1038/s414...
August 11, 2025 at 12:44 PM
🧬 New paper out! We explored why #antibodies have kept their 2 binding sites. Turns out, this “divalence” is crucial for B-cell receptors to sense antigen clustering and trigger immune responses, especially for weak or low-density antigens.
Check full text: doi.org/10.1038/s414...
Check full text: doi.org/10.1038/s414...
Reposted by Felipe Opazo
- A postdoc to work on cancer extravasation (4-year project).
- A postdoc help in completing Filopodia-related lab projects (2-year).
- Multiple Marie Curie postdoc positions that will open in the fall.
- We will open a 5-year position to cover my teaching (70% research and 30% teaching).
- A postdoc help in completing Filopodia-related lab projects (2-year).
- Multiple Marie Curie postdoc positions that will open in the fall.
- We will open a 5-year position to cover my teaching (70% research and 30% teaching).
Are you looking for a postdoc?
I will be looking for 2-4 people after the summer to join the lab !!
Do not hesitate to reach out and disseminate !!
I will be looking for 2-4 people after the summer to join the lab !!
Do not hesitate to reach out and disseminate !!
July 1, 2025 at 4:46 AM
- A postdoc to work on cancer extravasation (4-year project).
- A postdoc help in completing Filopodia-related lab projects (2-year).
- Multiple Marie Curie postdoc positions that will open in the fall.
- We will open a 5-year position to cover my teaching (70% research and 30% teaching).
- A postdoc help in completing Filopodia-related lab projects (2-year).
- Multiple Marie Curie postdoc positions that will open in the fall.
- We will open a 5-year position to cover my teaching (70% research and 30% teaching).
Reposted by Felipe Opazo
🌞🦙 Summer makeover? You bet.
Our alpacas ditched the fleece to stay cool – maybe your antibodies should too.
sdAbs are high-performance tools made of just the antigen-binding domain (∼15 kDa, 3 nm). 🔬🧪Perfect for IF, super-resolution microscopy, IP & more.
Browse our #nanobodies: nano-tag.com/shop/
Our alpacas ditched the fleece to stay cool – maybe your antibodies should too.
sdAbs are high-performance tools made of just the antigen-binding domain (∼15 kDa, 3 nm). 🔬🧪Perfect for IF, super-resolution microscopy, IP & more.
Browse our #nanobodies: nano-tag.com/shop/
June 11, 2025 at 10:27 AM
🌞🦙 Summer makeover? You bet.
Our alpacas ditched the fleece to stay cool – maybe your antibodies should too.
sdAbs are high-performance tools made of just the antigen-binding domain (∼15 kDa, 3 nm). 🔬🧪Perfect for IF, super-resolution microscopy, IP & more.
Browse our #nanobodies: nano-tag.com/shop/
Our alpacas ditched the fleece to stay cool – maybe your antibodies should too.
sdAbs are high-performance tools made of just the antigen-binding domain (∼15 kDa, 3 nm). 🔬🧪Perfect for IF, super-resolution microscopy, IP & more.
Browse our #nanobodies: nano-tag.com/shop/
Reposted by Felipe Opazo
💬Thank you, Dr. Maja Klevanski @majaklevanski.bsky.social (University of Heidelberg), for the great feedback on our recombinant anti-SARS-CoV-2 S1 antibody (rHcAb)! 😊 🔬 Applied in #dSTORM to study virion–cell interactions under antiviral conditions. Learn more about sdAb–Fc fusions 👉 bit.ly/3xdhYsn
June 4, 2025 at 8:44 AM
💬Thank you, Dr. Maja Klevanski @majaklevanski.bsky.social (University of Heidelberg), for the great feedback on our recombinant anti-SARS-CoV-2 S1 antibody (rHcAb)! 😊 🔬 Applied in #dSTORM to study virion–cell interactions under antiviral conditions. Learn more about sdAb–Fc fusions 👉 bit.ly/3xdhYsn
Reposted by Felipe Opazo
🤹♀️ Still juggling primary antibodies from different species?
Still stuck in sequential staining?
⏱️Switch to Smart Secondaries®🦙:
✅OneStep-IF
✅Same-species #multiplexing
✅Higher precision & reproducibility!
Choose smart 👉 bit.ly/3UZxRMI
#SecondaryNanobody #Microscopy #Immunofluorescence
Still stuck in sequential staining?
⏱️Switch to Smart Secondaries®🦙:
✅OneStep-IF
✅Same-species #multiplexing
✅Higher precision & reproducibility!
Choose smart 👉 bit.ly/3UZxRMI
#SecondaryNanobody #Microscopy #Immunofluorescence
May 28, 2025 at 12:30 PM
🤹♀️ Still juggling primary antibodies from different species?
Still stuck in sequential staining?
⏱️Switch to Smart Secondaries®🦙:
✅OneStep-IF
✅Same-species #multiplexing
✅Higher precision & reproducibility!
Choose smart 👉 bit.ly/3UZxRMI
#SecondaryNanobody #Microscopy #Immunofluorescence
Still stuck in sequential staining?
⏱️Switch to Smart Secondaries®🦙:
✅OneStep-IF
✅Same-species #multiplexing
✅Higher precision & reproducibility!
Choose smart 👉 bit.ly/3UZxRMI
#SecondaryNanobody #Microscopy #Immunofluorescence
Reposted by Felipe Opazo
@abberior.rocks — and NanoTag Biotechnologies rocks with them! 🤘
Smart Secondaries® 🦙now conjugated to abberior STAR dyes 🌈. Discover brighter, sharper, #STED -ready, species-independent #multiplexing & one-step IF. 🔬🤩
#SecondaryNanobodies
#SuperResolution
#FluorescenceMicroscopy
Smart Secondaries® 🦙now conjugated to abberior STAR dyes 🌈. Discover brighter, sharper, #STED -ready, species-independent #multiplexing & one-step IF. 🔬🤩
#SecondaryNanobodies
#SuperResolution
#FluorescenceMicroscopy
🔬 Small size, big impact – abberior STAR dyes meet Smart Secondaries® from NanoTag Biotechnologies @nanotag.bsky.social
We’re excited to launch bright, photostable, STED-optimized STAR dyes conjugated to high-precision single-domain antibodies (sdAbs, nanobodies).
#nanobodies #abberiordyes #STED
We’re excited to launch bright, photostable, STED-optimized STAR dyes conjugated to high-precision single-domain antibodies (sdAbs, nanobodies).
#nanobodies #abberiordyes #STED
May 20, 2025 at 2:04 PM
@abberior.rocks — and NanoTag Biotechnologies rocks with them! 🤘
Smart Secondaries® 🦙now conjugated to abberior STAR dyes 🌈. Discover brighter, sharper, #STED -ready, species-independent #multiplexing & one-step IF. 🔬🤩
#SecondaryNanobodies
#SuperResolution
#FluorescenceMicroscopy
Smart Secondaries® 🦙now conjugated to abberior STAR dyes 🌈. Discover brighter, sharper, #STED -ready, species-independent #multiplexing & one-step IF. 🔬🤩
#SecondaryNanobodies
#SuperResolution
#FluorescenceMicroscopy
Reposted by Felipe Opazo
Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...
May 16, 2025 at 8:00 AM
Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...
Reposted by Felipe Opazo
A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊
📄 bit.ly/4jTHfes
📄 bit.ly/4jTHfes
Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications
Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...
bit.ly
May 13, 2025 at 10:12 AM
A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊
📄 bit.ly/4jTHfes
📄 bit.ly/4jTHfes
Reposted by Felipe Opazo
Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!
January 20, 2025 at 10:22 AM
Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!
Reposted by Felipe Opazo
Fun fact: Alpacas love dust baths! 🦙💨
Spring at our farm means blossoms, sun & lots of happy rolling in the sand 🌸☀️
Just like our alpacas enjoy a refreshing dust bath, our clients enjoy smooth #sdAb discovery.
Let’s roll into new discoveries together 🙃!
#NanoTag #VHH #nanobody #AlpacaAntibodies
Spring at our farm means blossoms, sun & lots of happy rolling in the sand 🌸☀️
Just like our alpacas enjoy a refreshing dust bath, our clients enjoy smooth #sdAb discovery.
Let’s roll into new discoveries together 🙃!
#NanoTag #VHH #nanobody #AlpacaAntibodies
May 6, 2025 at 8:30 AM
Fun fact: Alpacas love dust baths! 🦙💨
Spring at our farm means blossoms, sun & lots of happy rolling in the sand 🌸☀️
Just like our alpacas enjoy a refreshing dust bath, our clients enjoy smooth #sdAb discovery.
Let’s roll into new discoveries together 🙃!
#NanoTag #VHH #nanobody #AlpacaAntibodies
Spring at our farm means blossoms, sun & lots of happy rolling in the sand 🌸☀️
Just like our alpacas enjoy a refreshing dust bath, our clients enjoy smooth #sdAb discovery.
Let’s roll into new discoveries together 🙃!
#NanoTag #VHH #nanobody #AlpacaAntibodies
Reposted by Felipe Opazo
Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩
We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨
We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨
Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution
We are excited to present our latest work published in @natcomms.nature.com
www.nature.com/articles/s41...
We are excited to present our latest work published in @natcomms.nature.com
www.nature.com/articles/s41...
Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications
Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...
www.nature.com
May 7, 2025 at 2:56 PM
Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩
We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨
We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨