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Reposted by Sarah Bigot

Guzzo lab - Lyon @mguzzo.bsky.social · Aug 24

Check out the first preprint from our lab describing a direct molecular cross-talk between (p)ppGpp and c-di-GMP nucleotide messengers! Great work from first-author Corentin Jaboulay who was a postdoc in our lab, and great collaborations! @mmsb-lyon.bsky.social @cnrsbiologie.bsky.social

bioRxiv Microbiology @biorxiv-microbiol.bsky.social · Aug 24

Cross-regulation of (p)ppGpp and c-di-GMP pathways controls a cell-cycle transition https://www.biorxiv.org/content/10.1101/2025.08.22.671821v1

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Reposted by Sarah Bigot

Cascales Lab @cascaleslab.bsky.social · 12d

Open position !!
Either at the Engineer or post-doctoral level, to work on filamentous phages. Collaboration between Laetitia Houot's group and Thierry Doan @thicoz.bsky.social.
To apply at the E level:
emploi.cnrs.fr/Offres/CDD/U...
To apply at the post-doc level:
emploi.cnrs.fr/Offres/CDD/U...

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Reposted by Sarah Bigot

Cascales Lab @cascaleslab.bsky.social · 12d

Happy to share our work on a T6SS effector with ADP ribose cyclase activity, published in JBC @asbmbjournals.bsky.social
Great work of Julius Martinkus @jmartinkus.bsky.social and (always) great collaboration with @dukasju.bsky.social and @laurentterradot.bsky.social
www.jbc.org/article/S002...

Widespread deployment of the human CD38 ADP-ribosyl cyclase fold in antibacterial and anti-eukaryotic polymorphic toxins

Bacterial polymorphic toxins are modular weapons that mediate inter-microbial competition and host interactions by delivering diverse cytotoxic domains through specialized secretion systems. Here, we ...

www.jbc.org

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Reposted by Sarah Bigot

Zamin Iqbal @zaminiqbal.bsky.social · 14d

Delighted to see our paper studying the evolution of plasmids over the last 100 years, now out! Years of work by Adrian Cazares, also Nick Thomson @sangerinstitute.bsky.social - this version much improved over the preprint. Final version should be open access, apols.
Thread 1/n

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Reposted by Sarah Bigot

Géraldine Laloux @lalouxlab.bsky.social · 15d

📝New preprint! #MicroSky

🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.

🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.

www.biorxiv.org/content/10.1...

Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle

The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...

www.biorxiv.org

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Sarah Bigot @sbigot.bsky.social · 22d

Bravo Agathe !!!

Reposted by Sarah Bigot

Agathe couturier @agathe-couturier.bsky.social · 23d

Exclusion systems, far from slowing down plasmid spread, are in fact essential to preserve host cell viability and thereby ensure the successful dissemination of conjugative plasmids and antibiotic resistance genes.

@nfrk92.bsky.social
@narjournal.bsky.social

academic.oup.com/nar/article/...

Exclusion systems preserve host cell homeostasis and fitness, ensuring successful dissemination of conjugative plasmids and associated resistance genes

Abstract. Plasmid conjugation is a major driver of antibiotic resistance dissemination in bacteria. In addition to genes required for transfer and maintena

academic.oup.com

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Reposted by Sarah Bigot

Géraldine Laloux @lalouxlab.bsky.social · 23d

🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
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Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference

Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.

academic.oup.com

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Reposted by Sarah Bigot

Yoann Santin @ysantin.bsky.social · 29d

I wrote a Preview in @cp-cellhostmicrobe.bsky.social !
A new study suggests that #curli may protect prey from bacterial predators like #Bdellovibrio.

Really looking forward to seeing this model confirmed with live-cell imaging and specific reporters!

www.cell.com/cell-host-mi...

When predators meet the matrix: Natural resistance via amyloid fibers

How do bacteria protect themselves against predators? In a recent issue of Nature, Ledvina et al. reveal curli fibers as a genetically encoded defense against Bdellovibrio predation in wild E. coli st...

www.cell.com

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Reposted by Sarah Bigot

Olaya Rendueles @olayarendueles.bsky.social · Sep 9

Check out @julielebris.bsky.social’s thread on our latest manuscript describing phenotypic heterogeneity in capsule production in Klebsiella & Acinetobacter @klebclub.bsky.social

This work started when I was still in @pasteur.fr & got finished in @cbitoulouse.bsky.social
#microsky #phagesky

Julie Le Bris @julielebris.bsky.social · Sep 5

Delighted to share our recently published work!
Ever wondered how Klebsiella (and others) deals with capsule production’s costs ?
The paper: journals.asm.org/doi/10.1128/...
Thread👇

Phenotypic heterogeneity of capsule production across opportunistic pathogens | mBio

The polysaccharidic capsule is present in ~50% of species across the bacterial phylogeny, including all ESKAPE microorganisms, the six most significant multidrug-resistant (MDR) nosocomial pathogens. It is also an important virulence factor and a major ...

journals.asm.org

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Reposted by Sarah Bigot

Zeynep Baharoglu @zeynepbaharoglu.bsky.social · Sep 6

So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!

Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters

Uridine boosts aminoglycoside treatment efficiency against antibiotic-susceptible as well as antibiotic-resistant E. coli strains.

www.science.org

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Reposted by Sarah Bigot

Anne Chevallereau @annechevallereau.bsky.social · Sep 5

Happy to see our paper out!

Functional assessment of anti-phage defence systems in their native clinical E. coli host
royalsocietypublishing.org/doi/10.1098/...

Thread in previous post 👇

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Reposted by Sarah Bigot

CNRS Biologie @cnrsbiologie.bsky.social · Sep 4

#ResultatScientifique🔎| Des scientifiques ont révélé comment le plasmide pOXA-48 rend les bactéries ultra-résistantes aux antibiotiques 🦠
🤝 @cnrs.fr @mmsb-lyon.bsky.social @cnrs-rhoneauvergne.bsky.social
✍️ @sbigot.bsky.social
📕 @natcomms.nature.com | buff.ly/u3Cy4Db

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Reposted by Sarah Bigot

Eduardo Rocha @epcrocha.bsky.social · Sep 3

At last ! A tool specifically for phage-plasmid hunters. Check tyPPing by @karinailchenko.bsky.social and @eugenpfeifer.bsky.social

Karina Ilchenko @karinailchenko.bsky.social · Sep 3

🚨 New preprint! 🧬

𝐏𝐡𝐚𝐠𝐞-𝐩𝐥𝐚𝐬𝐦𝐢𝐝𝐬 (𝐏-𝐏𝐬) are fascinating elements: both 𝐭𝐞𝐦𝐩𝐞𝐫𝐚𝐭𝐞 𝐩𝐡𝐚𝐠𝐞𝐬 and 𝐩𝐥𝐚𝐬𝐦𝐢𝐝𝐬 ➡️ tricky to detect.

We present 𝐭𝐲𝐏𝐏𝐢𝐧𝐠 — the first 𝐢𝐝𝐞𝐧𝐭𝐢𝐟𝐢𝐜𝐚𝐭𝐢𝐨𝐧 𝐚𝐧𝐝 𝐜𝐥𝐚𝐬𝐬𝐢𝐟𝐢𝐜𝐚𝐭𝐢𝐨𝐧 𝐭𝐨𝐨𝐥 🛠️designed specifically for P-Ps:
✅ Accurate
✅ Sensitive
✅ Easy to use

📖 www.biorxiv.org/content/10.1...

Efficient detection and typing of phage-plasmids

Phage-plasmids are temperate phages that replicate as plasmids during lysogeny. Despite their high diversity, they carry genes similar to phages and plasmids. This leads to gene exchanges, and to the ...

www.biorxiv.org

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Reposted by Sarah Bigot

David Sünderhauf @davvi36.bsky.social · Sep 2

PhD opportunity, please share:

We are looking for a prospective PhD student to start in October 2026 who is excited about bacteria (Klebsiella pneumoniae), how they interact, and how they exchange DNA.
All details can be found on the funder website 👉 gw4biomed.ac.uk/developing-c...

Developing CRISPR-Cas antimicrobials to tackle antibiotic resistance spread in Klebsiella pneumoniae - GW4 BioMed MRC DTP

Project Code MRCIIAR26Ex van Houte Project Type Wet lab Research Theme Infection, Immunity, Antimicrobial Resistance and Repair Project Summary Download Summary Antimicrobial resistance (AMR) poses a ...

gw4biomed.ac.uk

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Reposted by Sarah Bigot

Jan-Willem Veening @veeninglab.com · Sep 3

Cool preprint by Mathieu Berge @polardlab.bsky.social showing that the Spr1630 toxin (RipA) toxin targets the replication sliding-clamp (DnaN) and that this induces competence in Streptococcus pneumoniae. www.biorxiv.org/content/10.1...

A toxin/antitoxin system targeting the replication sliding-clamp induces competence in Streptococcus pneumoniae.

Streptococcus pneumoniae is a pathogenic bacterium capable of entering a cellular differentiation state, called competence, which enables it to acquire new genetic functions by natural transformation,...

www.biorxiv.org

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Reposted by Sarah Bigot

Jana Huisman @jhuisman.bsky.social · Aug 25

Have you ever wondered what increasing environmental stress will do to microbial communities?

In our new preprint, @martinadalbello.bsky.social, Jeff Gore and I studied the impact of salinity on microbial community composition and function. 🧵 (1/5)
www.biorxiv.org/content/10.1...

Microbial communities demonstrate robustness in stressful environments due to predictable composition shifts

Environmental stress reduces species growth rates, but its impact on the function of microbial communities is less clear. Here, we experimentally demonstrate that increasing salinity stress shifts com...

www.biorxiv.org

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Reposted by Sarah Bigot

Jerónimo Rodríguez-Beltrán @jerorb.bsky.social · Jul 2

🚨🚨New paper out in @natcomms.nature.com!!

Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬

👉 www.nature.com/articles/s41...

Universal rules govern plasmid copy number - Nature Communications

Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...

www.nature.com

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Reposted by Sarah Bigot

Alvaro San Millan @sanmillan.bsky.social · Aug 20

New paper out!

Alicia Calvo-Villamañán @aliciapcv.bsky.social · Aug 20

This work is finally published! 🥳🧬
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14

Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens

Nature Communications - This study investigates the effects of the carbapenem resistance plasmid pOXA-48 in clinical enterobacteria. Using CRISPRi screens, the authors revealed that the...

rdcu.be

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Sarah Bigot @sbigot.bsky.social · Aug 20

Our study is back-to-back with @sanmillan.bsky.social’s lab. Highly recommend their paper extending the pOXA-48 work to clinical pathogens 👉 www.nature.com/articles/s41...

Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens - Nature Communications

This study investigates the effects of the carbapenem resistance plasmid pOXA-48 in clinical enterobacteria. Using CRISPRi screens, the authors revealed that the carbapenemase OXA-48 has a role in the...

www.nature.com

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Sarah Bigot @sbigot.bsky.social · Aug 20

Our paper “Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli” is now online in @natcomms.nature.com ✨
👉 www.nature.com/articles/s41...
@cnrs-rhoneauvergne.bsky.social @mmsb-lyon.bsky.social

Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli - Nature Communications

pOXA-48 plasmids have emerged as key vectors of carbapenem resistance within Enterobacteriaceae. In this study, the authors use a transposon sequencing (Tn-seq) approach to identify genetic determinan...

www.nature.com

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Reposted by Sarah Bigot

DaganLab @daganlab.bsky.social · Aug 3

Happy to finally share this! Read here about the best strategy the the persistence of low-copy plasmids. @mariosanter.bsky.social @iwangtoknow.bsky.social doi.org/10.1038/s414...

The combination of active partitioning and toxin-antitoxin systems is most advantageous for low-copy plasmid fitness - Nature Communications

Effe et al. evaluate how specific plasmid traits influence plasmid fitness; they provide mechanistic insight into the persistence of large conjugative plasmids, including those that disseminate antibi...

doi.org

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Reposted by Sarah Bigot

Alvaro San Millan @sanmillan.bsky.social · Jul 21

Our latest paper explores the factors shaping plasmid-bacteria associations and highlights why studying plasmid-host interactions of clinical relevance is key for understanding the evolution and spread of AMR.

Find out more 👉 plasmidlab.es/publications/

#AntibioticResistance

Publications - Plasmidlab

Take a detailed look at our work Discover our latest results Our story on bacterial evolution research Explore our publication’s archive Browse the list below or enter our PubMed’s repository to…

plasmidlab.es

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Reposted by Sarah Bigot

Géraldine Laloux @lalouxlab.bsky.social · Jul 16

We wrote 2 📕 chapters with tips & tricks to identify protein-protein interactions in bacteria 🦠

1) In vivo proximity labeling with miniTurbo link.springer.com/protocol/10....

2) An adapted polar recruitment assay feat. E. coli as a test tube link.springer.com/protocol/10.....

Enjoy #MicroSky 🧪

a man wearing sunglasses and a white t-shirt is holding something in his hands .

ALT: a man wearing sunglasses and a white t-shirt is holding something in his hands .

media.tenor.com

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Reposted by Sarah Bigot

Yoann Santin @ysantin.bsky.social · Jul 14

Excited to share our newly book chapter on using #miniTurbo proximity labeling in #bacteria
We wrote it as a hands-on guide for anyone looking to get started with this approach.
Congrats to @coralietesseur.bsky.social and Ophélie Remy 👏 @lalouxlab.bsky.social
link.springer.com/protocol/10....

Identification of In Vivo Protein Networks Using miniTurbo Proximity Labeling in Bacteria

Protein–protein interactions play a crucial role in many bacterial cell processes. miniTurbo-based proximity labeling has emerged as a powerful tool for the identification of in vivo protein net...

link.springer.com

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