László Tora
@tlaszlo.bsky.social
Transcription regulation, how genes are turned on or off, transcriptional cofactors, general transcription factors and core promoters.
Reposted by László Tora
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Enhancer-promoter compatibility is mediated by the promoter-proximal region
Gene promoters induce transcription in response to distal enhancers. How enhancers specifically activate their target promoter while bypassing other promoters remains unclear. Here, we find that the p...
www.biorxiv.org
October 16, 2025 at 3:06 PM
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Reposted by László Tora
Today it was announced that Sir John Gurdon passed away.
His ground-breaking discovery was that differentiated cells can be reprogrammed. He was a creative and distinguished developmental biologist, a kind person with a great inquisitive mind and original ideas
www.cam.ac.uk/research/new...
His ground-breaking discovery was that differentiated cells can be reprogrammed. He was a creative and distinguished developmental biologist, a kind person with a great inquisitive mind and original ideas
www.cam.ac.uk/research/new...
Nobel Laureate Professor Sir John Gurdon dies aged 92
It is with great sadness that the University shares the news of the death of Professor Sir John Gurdon, founder of the Gurdon Institute.
www.cam.ac.uk
October 8, 2025 at 11:56 AM
Today it was announced that Sir John Gurdon passed away.
His ground-breaking discovery was that differentiated cells can be reprogrammed. He was a creative and distinguished developmental biologist, a kind person with a great inquisitive mind and original ideas
www.cam.ac.uk/research/new...
His ground-breaking discovery was that differentiated cells can be reprogrammed. He was a creative and distinguished developmental biologist, a kind person with a great inquisitive mind and original ideas
www.cam.ac.uk/research/new...
Reposted by László Tora
With self-deprecating humor, Sir John loved to tell the story of his Biology teacher (1949)
October 8, 2025 at 12:10 PM
With self-deprecating humor, Sir John loved to tell the story of his Biology teacher (1949)
Do not forget to apply to the FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics (19–22 October 2026, Budapest, Hungary. Will be a great meeting as always. Check out the site: network.febs.org/posts/febs-a...
FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics
The FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics will take place 19–22 October 2026 in Budapest, Hungary. Here we give a taste of the scientific content, flag some of the speaker...
network.febs.org
September 10, 2025 at 2:24 PM
Do not forget to apply to the FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics (19–22 October 2026, Budapest, Hungary. Will be a great meeting as always. Check out the site: network.febs.org/posts/febs-a...
Reposted by László Tora
Reposted by László Tora
"In our view, it [hexanediol] should thus be avoided and not be used as evidence for the formation of an assembly via phase separation or the fluid-like nature of a condensate in vitro or in vivo." doi.org/10.1038/s414...
Current practices in the study of biomolecular condensates: a community comment - Nature Communications
The realization that the cell is abundantly compartmentalized into biomolecular condensates has opened new opportunities for understanding the physics and chemistry underlying many cellular processes1...
doi.org
August 21, 2025 at 3:38 PM
"In our view, it [hexanediol] should thus be avoided and not be used as evidence for the formation of an assembly via phase separation or the fluid-like nature of a condensate in vitro or in vivo." doi.org/10.1038/s414...
Read this interesting preprint from the lab of Krishnamurthy Natarajan: Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen Candida albicans
doi: doi.org/10.1101/2025...
doi: doi.org/10.1101/2025...
Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen Candida albicans
The fidelity of assembly of multiprotein complexes is essential for the formation of stable and functional protein complexes that are critical for cell growth and survival. In this context, TBP-associ...
doi.org
August 29, 2025 at 3:24 PM
Read this interesting preprint from the lab of Krishnamurthy Natarajan: Cotranslational assembly confers specificity for in vivo target heterodimerization of paralogous H2B-like TAF12 proteins in the human fungal pathogen Candida albicans
doi: doi.org/10.1101/2025...
doi: doi.org/10.1101/2025...
Reposted by László Tora
One more movie!
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)
August 29, 2025 at 12:36 AM
One more movie!
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)
Check out our new collaborative preprint about Affinity-ligand purification of native human low-abundance multi-protein complexes doi.org/10.1101/2025...
Affinity-ligand purification of native human low-abundance multi-protein complexes for structure determination
In human cells, large multi-protein transcription co-activators, such as chromatin remodelers or histone acetyltransferases, play critical roles in gene-expression regulation and are often implicated ...
doi.org
August 12, 2025 at 7:30 AM
Check out our new collaborative preprint about Affinity-ligand purification of native human low-abundance multi-protein complexes doi.org/10.1101/2025...
Reposted by László Tora
Exquisite work. Well done to the whole team.
How to find Evolutionary Conserved Enhancers in 2025? 🐣-🐭
Check out our paper - fresh off the press!!!
We find widespread functional conservation of enhancers in absence of sequence homology
Including: a bioinformatic tool to map sequence-diverged enhancers!
rdcu.be/enVDN
github.com/tobiaszehnde...
Check out our paper - fresh off the press!!!
We find widespread functional conservation of enhancers in absence of sequence homology
Including: a bioinformatic tool to map sequence-diverged enhancers!
rdcu.be/enVDN
github.com/tobiaszehnde...
Conservation of regulatory elements with highly diverged sequences across large evolutionary distances
Nature Genetics - Combining functional genomic data from mouse and chicken with a synteny-based strategy identifies positionally conserved cis-regulatory elements in the absence of direct sequence...
rdcu.be
May 28, 2025 at 5:33 AM
Exquisite work. Well done to the whole team.
Reposted by László Tora
🚨 New preprint out! Do you think Single Molecule Footprinting and Fiber-seq are super cool but aren't sure how to unlock their full potential? HiddenFoot can help you: www.biorxiv.org/content/10.1...
May 17, 2025 at 5:00 PM
🚨 New preprint out! Do you think Single Molecule Footprinting and Fiber-seq are super cool but aren't sure how to unlock their full potential? HiddenFoot can help you: www.biorxiv.org/content/10.1...
Friday we celebrated my 40 years of scientific career in France, with present and past lab members and many friends. It was a fantastic day full of emotions, memories and great science. Thanks for making the day so memorable !
May 1, 2025 at 7:08 AM
Friday we celebrated my 40 years of scientific career in France, with present and past lab members and many friends. It was a fantastic day full of emotions, memories and great science. Thanks for making the day so memorable !
Reposted by László Tora
The importance of moving away from bulk! Occupancy of Pol II at promoters is dramatically different between fly and mouse cells! When looking single molecule! Proud of the team! @kasitc.bsky.social @molinalab.bsky.social doi.org/10.1038/s443...
April 2, 2025 at 8:47 AM
The importance of moving away from bulk! Occupancy of Pol II at promoters is dramatically different between fly and mouse cells! When looking single molecule! Proud of the team! @kasitc.bsky.social @molinalab.bsky.social doi.org/10.1038/s443...
Reposted by László Tora
Two more weeks to send us your abstract! >20 abstract selected talks. Priority to junior scientists and cool biology on gene regulation (DNA/RNA/protein). Come to meet the world leaders in single molecule genomics and single molecule imaging! Help shaping the future of the field in the workshops!
March 26, 2025 at 12:17 PM
Two more weeks to send us your abstract! >20 abstract selected talks. Priority to junior scientists and cool biology on gene regulation (DNA/RNA/protein). Come to meet the world leaders in single molecule genomics and single molecule imaging! Help shaping the future of the field in the workshops!
Read our collaborative paper
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.
March 21, 2025 at 5:27 PM
Read our collaborative paper
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.
@longchrom.bsky.social and @molinalab.bsky.social
showing that disruption of NuA4 and ATAC coactivator complexes cause gene-specific transcript buffering, suggesting that cells dynamically adjust RNA export/degradation to sustain cellular homeostasis.
Reposted by László Tora
Reposted by László Tora
Friends! The science that we love is under threat. World-wide, there're forces seeking to make it corporate-like, focused on deliverables, or even the enemy of society. Take a stand & celebrate science & music! #theconferencetoendallconferences - Registration starts NOW!
woodstock.img.cas.cz
woodstock.img.cas.cz
March 1, 2025 at 5:44 PM
Friends! The science that we love is under threat. World-wide, there're forces seeking to make it corporate-like, focused on deliverables, or even the enemy of society. Take a stand & celebrate science & music! #theconferencetoendallconferences - Registration starts NOW!
woodstock.img.cas.cz
woodstock.img.cas.cz
Reposted by László Tora
Thrilled to share our collaborative work on the structural basis and myeloma disease link of the midnolin-proteasome pathway with Chris Nardone from the Elledge lab, Jingjing Gao from the Shao lab @sshaolab.bsky.social and all the co-authors who put in the hard work! www.biorxiv.org/content/10.1...
Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma
The midnolin-proteasome pathway degrades many nuclear proteins without ubiquitination, but how it operates mechanistically remains unclear. Here, we present structures of the midnolin-proteasome compl...
www.biorxiv.org
February 24, 2025 at 2:11 AM
Thrilled to share our collaborative work on the structural basis and myeloma disease link of the midnolin-proteasome pathway with Chris Nardone from the Elledge lab, Jingjing Gao from the Shao lab @sshaolab.bsky.social and all the co-authors who put in the hard work! www.biorxiv.org/content/10.1...
Reposted by László Tora
‼️ This Wednesday! Don’t forget to register as we host three amazing ECR scientists!
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social
⚡️Our recurring registration link:
us06web.zoom.us/webinar/regi...
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social
⚡️Our recurring registration link:
us06web.zoom.us/webinar/regi...
February 23, 2025 at 10:13 PM
‼️ This Wednesday! Don’t forget to register as we host three amazing ECR scientists!
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social
⚡️Our recurring registration link:
us06web.zoom.us/webinar/regi...
@manucazottes.bsky.social @robertadolling1.bsky.social
@leandrosboukas.bsky.social
⚡️Our recurring registration link:
us06web.zoom.us/webinar/regi...
Reposted by László Tora
Interesting piece on the role of intrinsically disordered regions in #transcriptionfactor specificity, target search etc. ➡️ www.nature.com/articles/s41... #generegulation
Intrinsically disordered regions as facilitators of the transcription factor target search - Nature Reviews Genetics
Intrinsically disordered domains (IDRs) are increasingly appreciated as important components of protein function. This Perspective discusses the emerging evidence for IDRs from transcription factors a...
www.nature.com
February 22, 2025 at 7:48 AM
Interesting piece on the role of intrinsically disordered regions in #transcriptionfactor specificity, target search etc. ➡️ www.nature.com/articles/s41... #generegulation
Reposted by László Tora
Search gets faster, binding longer, bound fraction of cohesin and CTCF goes up, especially at and after ZGA
February 21, 2025 at 9:20 AM
Search gets faster, binding longer, bound fraction of cohesin and CTCF goes up, especially at and after ZGA
Reposted by László Tora
Single molecules, in living zebrafish embryos, during hour-long period of development: Jonas' work on binding kinetics of cohesin and CTCF around ZGA now online. Great collab with labs of @lucagiorgetti.bsky.social @akispapantonis.bsky.social
doi.org/10.1038/s414...
doi.org/10.1038/s414...
Increasingly efficient chromatin binding of cohesin and CTCF supports chromatin architecture formation during zebrafish embryogenesis - Nature Communications
Using single-molecule tracking of cohesin and CTCF during zebrafish embryogenesis, the molecular kinetics underlying chromatin architecture formation was revealed and gradual emergence of chromatin st...
doi.org
February 21, 2025 at 9:17 AM
Single molecules, in living zebrafish embryos, during hour-long period of development: Jonas' work on binding kinetics of cohesin and CTCF around ZGA now online. Great collab with labs of @lucagiorgetti.bsky.social @akispapantonis.bsky.social
doi.org/10.1038/s414...
doi.org/10.1038/s414...
Reposted by László Tora
Program is out for our first EMBL #singlemolecule gene regulation meeting! Check out the dedicated panel sessions on single molecule genomics, single molecule imaging and theory where we would like to brainstorm the future of the field:
Registration is open! 👇
www.embl.org/about/info/c...
Registration is open! 👇
www.embl.org/about/info/c...
Gene regulation: one molecule at a time
www.embl.org
February 21, 2025 at 9:58 AM
Program is out for our first EMBL #singlemolecule gene regulation meeting! Check out the dedicated panel sessions on single molecule genomics, single molecule imaging and theory where we would like to brainstorm the future of the field:
Registration is open! 👇
www.embl.org/about/info/c...
Registration is open! 👇
www.embl.org/about/info/c...
Reposted by László Tora
The latest publication from our lab is online in @narjournal.bsky.social . Congratulations to @marcelwerner.bsky.social and Manuel Trauner for developing a novel system in mammalian cells to study Transcription-Replication conflicts and pointing DOT1L as a transcription mediator at TRC sites!
I am delighted to share the main work of my PhD project in the @hamperllab.bsky.social now out in @narjournal.bsky.social. TRCs drive R-loop-dependent nucleosome eviction and require DOT1L activity for transcription recovery.
academic.oup.com/nar/article/...
#TRC #r-loop #chromatin
More below 👇
academic.oup.com/nar/article/...
#TRC #r-loop #chromatin
More below 👇
Transcription-replication conflicts drive R-loop-dependent nucleosome eviction and require DOT1L activity for transcription recovery
Abstract. Progressing transcription and replication machineries profoundly impact their underlying chromatin template. Consequently, transcription-replicat
academic.oup.com
February 20, 2025 at 3:45 PM
The latest publication from our lab is online in @narjournal.bsky.social . Congratulations to @marcelwerner.bsky.social and Manuel Trauner for developing a novel system in mammalian cells to study Transcription-Replication conflicts and pointing DOT1L as a transcription mediator at TRC sites!
Reposted by László Tora
The first paper from our lab is online! A wonderful collaboration with @scottbscience.bsky.social lab. We discover that Pol II is extensively regulated by Ubiquitin at the beginning of the transcription cycle!
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
CRL3ARMC5 ubiquitin ligase and Integrator phosphatase form parallel mechanisms to control early stages of RNA Pol II transcription
Cacioppo, Gillis, Shlamovitz, Zeller, et al. show that CRL3ARMC5 ubiquitylates RNA Pol II at early stages of transcription as part of a major homeostatic RNA Pol II turnover mechanism. CRL3ARMC5 and the Integrator phosphatase act as complementary pathways, monitoring the quantity and quality of RNA Pol II complexes before they are licensed into elongation.
www.cell.com
December 11, 2024 at 5:19 PM
The first paper from our lab is online! A wonderful collaboration with @scottbscience.bsky.social lab. We discover that Pol II is extensively regulated by Ubiquitin at the beginning of the transcription cycle!
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...