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Reposted by Albert Blanch-Asensio

Alessandro Bertero @berterolab.bsky.social · 18d

💸 Stem cell culture that’s ~95% cheaper and weekend-free: a win-win for PIs and trainees!
Our revised manuscript is online at ORE: open-research-europe.ec.europa.eu/articles/4-1...

Benchmarked across cell lines & differentiation protocols, including scRNA-seq — with lessons for #cultivatedmeat too!

open-research-europe.ec.europa.eu

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Reposted by Albert Blanch-Asensio

Axelle Bois @axellebois.bsky.social · Jan 24

Our review, "Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction." doi.org/10.1038/s415..., has just been published in npj Regenerative Medicine!

Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction - npj Regenerative Medicine

npj Regenerative Medicine - Revitalizing the heart: strategies and tools for cardiomyocyte regeneration post-myocardial infarction

doi.org

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Reposted by Albert Blanch-Asensio

Oliver Harschnitz @harschnitz.bsky.social · Dec 15

Created a second stem cell biology starter pack, as the first one was full.

Let me know if you would like to be added!

go.bsky.app/UG3eYwU

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Reposted by Albert Blanch-Asensio

Benjamin B Johnson @benjaminbjohnson.bsky.social · Dec 13

How to fix a broken #heart 💔->❤️

#Proteoglycan rich #ECMatrix scar tissue is laid by migratory cells after a cardiac scratch wound with #hiPSC #Cardiomyocytes.

Happy #Fluorescencefriday #BlueSky & #CardioSky!

@ablanchasensio.bsky.social 👀
#Science 💥🧪 #Microscopy 🔬

Cardiac scratch wound assay to show the role of ECM in wound healing. HiPSC Cardiomyocytes in white on the boarders with a rich ECM scar tissues (cyan) through the middle with a simple nuclear stain (red) highlighting all cells.

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 13

With Christmas just around the corner, it’s time for the #FluorescenceFriday post🎄!

This week, we’re featuring a hiPSC reporter line engineered to express 2 fluorescent proteins—located in the nuclei and actin filaments of #hiPSCs, generated using STRAIGHT-IN Dual🧪🔬

@benjaminbjohnson.bsky.social?

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Reposted by Albert Blanch-Asensio

Maik Bischoff @maikbischoff.bsky.social · Nov 27

There are so many #morphogenesis researchers here!
My first starter pack is full!
Here is Part 2: go.bsky.app/K6YYKeC

#devbio #cellbio #science

Tell me if I should add you but try to make sure, that you're not already in Part 1.
I cannot cross-reference everyone 😅

Part 1: go.bsky.app/RzDD9or

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Reposted by Albert Blanch-Asensio

Ina Klockner 🧬👩‍🔬🔬 @inaklockner.bsky.social · Nov 20

New starter pack for all people interested in Gene Editing & Gene Therapy 🧬✂️ including Tools & Delivery|CRISPR-Cas|Zinc finger nucleases|Viral vectors|Non-viral delivery methods

go.bsky.app/8R4dVez

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 8

Can I be added here, please? Thanks!

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Reposted by Albert Blanch-Asensio

Benjamin B Johnson @benjaminbjohnson.bsky.social · Dec 5

Created a starter pack focusing on disease modeling in a dish using stem cells! 🧪🔬

Please (self)nominate anyone you think should be added to the list and repost so it finds the right people ❤️

hPSC Disease Modeling: go.bsky.app/EpWsxNG

For a good general stem cell pack go here: go.bsky.app/UTW4d7b

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 8

Can I also be added please? Thanks!

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Reposted by Albert Blanch-Asensio

Emily R. Trunnell, Ph.D. @ertrunnell.bsky.social · Nov 19

Working on a #StarterPack to collect researchers using human biology-based tools ( #organoids, #microphysiologicalsystems, other human-derived #invitro tools) + computational models & the orgs & accounts that promote non-animal science. Small so far, but I'm new here. Msg to add.
go.bsky.app/SVWGwgu

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 8

Hi! Can I be added please? Gracias!

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Reposted by Albert Blanch-Asensio

Benjamin B Johnson @benjaminbjohnson.bsky.social · Dec 6

Dedicating this #Fluorescencefriday to the #ECMatrix lovers. Was trialing some ECM staining methods on a scratch wound and love the HABP staining looking almost like flames 🔥🧪🔬

Lets see what you got, @ablanchasensio.bsky.social 😉

Cyan cellular extracellular matrix fibers are highlighted laying over the top of the orange hyaluronan secreted matrix after a scratch would assay.

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 8

Can I also be added please? Thanks!

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 7

Added!

Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 6

Same, but in 3D cardiac organoids 🚦🔴

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 6

Calcium signals in beating hiPSCs-derived cardiomyocytes.
A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus.
doi.org/10.1016/j.cr...
doi.org/10.1101/2024...

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 6

Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social

Genetically-modified hiPSCs expressing the genetically-encoded calcium indicator jRCaMP1b with a nuclear export signal (NES). This reporter is used to track calcium dynamics in live cells. It combines a red fluorescent protein with calmodulin and a calcium-binding domain, changing fluorescence intensity upon calcium binding. Its red-shifted emission is ideal for imaging in complex tissues or dual-color experiments, making it valuable for studying calcium-mediated processes.
A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus. See below!
https://doi.org/10.1016/j.crmeth.2022.100300
https://doi.org/10.1101/2024.10.17.616637

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 5

My colleague @benjaminbjohnson.bsky.social has just created a starter pack for hPSC disease modeling. Check it out! 🧪🧫

Benjamin B Johnson @benjaminbjohnson.bsky.social · Dec 5

Created a starter pack focusing on disease modeling in a dish using stem cells! 🧪🔬

Please (self)nominate anyone you think should be added to the list and repost so it finds the right people ❤️

hPSC Disease Modeling: go.bsky.app/EpWsxNG

For a good general stem cell pack go here: go.bsky.app/UTW4d7b

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Dec 4

🧪

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Reposted by Albert Blanch-Asensio

Benjamin B Johnson @benjaminbjohnson.bsky.social · Dec 4

🔥Super excited to drop our recent preprint🔥

biorxiv.org/cgi/content/...

Here, we automated the generation of 3D Cardiac Microtissues and performed a small high-throughput screen to identify several novel anti-arrhythmic compounds for CPVT1 🧪❤️

#Cardiosky #Science #hiPSC #Arrhythmia #Stemcell

Graphical abstract depicts automated setup to generate 3D cardiac mini tissues consisting of 3 cell types seeded into 384 well plates. The cardiac tissues are monitored using calcium traces to detect arrhythmias and subsequent rescue after compound addition.

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Reposted by Albert Blanch-Asensio

John Ngo @johntngo.bsky.social · Nov 30

We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs.

You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Nov 29

that is right!

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Nov 29

Hey #BlueSky! Here is my first #FluorescenceFriday post!
This cardiac organoid was derived from #hiPSC and imaged using light sheet #microscopy 🔬.

🔵 Cardiomyocytes
🔴 Endothelial cells
🟡 Fibroblasts

More coming next week! 🧪

#Science #Biology #CellBiology #SciArt
@benjaminbjohnson.bsky.social

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Albert Blanch-Asensio @ablanchasensio.bsky.social · Nov 28

Still a few spots left in the Mammalian Synthetic Biology starter pack!🧪

Albert Blanch-Asensio @ablanchasensio.bsky.social · Nov 20

Please share!

Here is a starter pack dedicated to Mammalian SynBio!

It is still incomplete so if you would like to be added/removed please let me know!

go.bsky.app/SsxHKoV

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