Fillip Port
@crisprflydesign.bsky.social
2.3K followers 890 following 260 posts
Scientist at the German Cancer Reseach Center in Heidelberg. We develop genome engineering tools for functional genomics. #CRISPR #Drosophila #openscience
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crisprflydesign.bsky.social
Our new preprint is out - please share!

We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.

Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
crisprflydesign.bsky.social
Deutsche Bahn has entered the chat.... 🙄🤣
Reposted by Fillip Port
juliusbrennecke.bsky.social
Love RNA biology?

Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).

PhD student/postdoc position co-supervised by Clemens Plaschka & myself.

DM or email us if you’d like to know more!

@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
juliusbrennecke.bsky.social
PIWI clade Argonautes are essential for transposon silencing. Without them, animals are sterile due to massive transposon activity.

But how does piRNA-guided target interaction translate into silencing?

PhD student Júlia Portell Montserrat has an intriguing answer

www.cell.com/molecular-ce...
crisprflydesign.bsky.social
High levels of Cas9 are toxic in sensory neurons. Reducing Cas9 levels with uORFs avoids toxicity and is compatible with efficient editing. From @thompsonpeerlab.bsky.social. Fly lines @vdrc-flies.bsky.social

#CRISPR #Drosophila

www.biorxiv.org/content/10.1...
Figure from the preprint showing sensory neuron morphology with different levels of Cas9 expression.
Reposted by Fillip Port
justinhendrix.bsky.social
Macron remarks are notable- some quotes: "We have been incredibly naive in entrusting our democratic space to social networks that are controlled either by large American entrepreneurs or large Chinese companies, whose interests are not at all the survival or proper functioning of our democracies."
defenddemocracy.bsky.social
President Macron: “Europeans, let's wake up!

We have been incredibly naive in entrusting our democratic space to social networks.”

defenddemocracy.eu/macron-democ...
Reposted by Fillip Port
kwolbachia.bsky.social
I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
crisprflydesign.bsky.social
What an exciting opportunity! Setting up a new lab with the great @madalenamrp.bsky.social at one of the best institutes around. Apply! 🧪🐟🧬
mpi-cbg.de
Are you interested in a PhD in the area of "Mechanisms of translational control during embryogenesis?" Apply now for a PhD @mpi-cbg.de! Overview of all participating RGLs: imprs.mpi-cbg.de/5761/researc...
Reposted by Fillip Port
gautamdey.bsky.social
If you’re applying to your dream lab for an internship/PhD/postdoc, always send a second email 1-2 weeks after the first one if you don’t hear back.

I promise we will be grateful rather than annoyed. My email inbox is a disaster and I’m quite junior - and very few of us have secretarial support
Reposted by Fillip Port
maikbischoff.bsky.social
My entry for today’s #FluorescenceFriday: a pupal #Drosophila testis with muscles expressing
🔵 lifeact &
🔴 RFP-nls

Honored & grateful to receive an honorable mention at @healthcare.nikon.com Nikon Small World 🌍🔬✨

🔗 www.nikonsmallworld.com/galleries/20...

#NikonSmallWorld #Microscopy #ScienceArt
Reposted by Fillip Port
joachimgoedhart.bsky.social
Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...
Figure 1: Fluorophore intensity in the Drosophila blastoderm (n.c. 14). Comparison of (A) green and (B) red fluorescence intensity using the same intensity scaling in n.c. 14. The fluorescence signal did
not saturate. Shown are single imaging planes. Normalised histograms of fluorescence intensity for green (C) and red (D) fluorophores averaged across at least n=3 embryos per line. Scale bars = 20 μm
Reposted by Fillip Port
martinholab.bsky.social
Quick Drosophila question: I’m planning to integrate a construct on the second chromosome but would like to avoid the attP40 landing site. Any suggestion for a good alternative attP site on chromosome II? It’s for a skeletal-muscle–related project.
Thanks! 🙂
Rui
crisprflydesign.bsky.social
We've been using attP-JK22C on some occasions. Integration frequency ok. Very light eye colour, so probably low eye expression, but robust expression in the wing disc. But have not used with a muscle driver, so lacking the data relevant to you.
crisprflydesign.bsky.social
Congratulations, David! All the best for this new chapter.
Reposted by Fillip Port
fly-eds.bsky.social
The @fly-eds.bsky.social is having its general assembly this saturday at 13:30 at #EDRC2025 , please join us to discuss our society and to find out more about @flybase.bsky.social and the current funding crisis
Reposted by Fillip Port
kakape.bsky.social
‘On 18 August, judicial police showed up at the Research Institute in Health Sciences (IRSS) in Bobo-Dioulasso, a key partner in Target Malaria, to stage what scientists described as a “brutal, humiliating” raid.’

My story on mosquitoes, malaria and misinformation in @science.org 🧪
After ‘humiliating’ raid, Burkina Faso halts ‘gene drive’ project to fight malaria
Disinformation campaign may have triggered “brutal” shutdown of mosquito lab
www.science.org
Reposted by Fillip Port
seemagrewal.bsky.social
Huge thanks to all of our peer reviewers! Thanks to your efforts, we’ve helped to restore and preserve over 10,000 trees in ancient woodland! 🌳
#PeerReviewWeek
#PRW2025
#notforprofit
#ForestofBiologists
biologists.bsky.social
In our Forest of Biologists we fund the restoration and preservation of ancient woodland and dedicate these trees to our peer reviewers.

There are now over 10,000 trees for peer reviews!

Thank you to all our peer reviewers. Find out more:
forest.biologists.com

#PRW2025 @peerreviewweek.bsky.social
Lots of illustrated trees. A sample image taken from the Forest of Biologists website.
Reposted by Fillip Port
johntngo.bsky.social
🚀 Our new paper is out @natmethods.nature.com!

Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.

🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Reposted by Fillip Port
forscherrobert.bsky.social
Ich suche eine*n TA für meine Arbeitsgruppe!

Gern weitersagen 🤗🌾

#plantscijob
leibnizipk.bsky.social
Sie haben eine Ausbildung als Biologielaborant*in oder Biologisch-technische*r Assistent*in? Und Sie haben
Erfahrungen in der molekularbiologischen Laborarbeit, insbesondere PCR und Klonierungen? Dann haben wir am @leibnizipk.bsky.social ein spannendes Jobangebot!

➡️Alle Infos: tinyurl.com/2rkbby5t
Reposted by Fillip Port
juliusbrennecke.bsky.social
PIWI clade Argonautes are essential for transposon silencing. Without them, animals are sterile due to massive transposon activity.

But how does piRNA-guided target interaction translate into silencing?

PhD student Júlia Portell Montserrat has an intriguing answer

www.cell.com/molecular-ce...
Reposted by Fillip Port
felixschmidtner.bsky.social
Wegen den Einschnitten der US-Regierung fürchten sich Wissenschaftler, dass die Flybase, eine zentrale wissenschaftliche Datenbank für die Forschung an der Fruchtfliege Drosophila melanogaster, verloren gehen könnte. Mein Text dazu in der heutigen F.A.Z.
Bild einer Zeitung. Ein Bild mit einer fetten Fliege mit roten Augen. Überschrift: Notruf der Fliege. Lead: Erkenntnisse aus der Drosophilaforschung werden in einer Datenbank gesammelt. Deren Finanzierung steht vor dem Aus. Wegen Trump.
Reposted by Fillip Port
gain4crops.eu
Robert Hoffie @forscherrobert.bsky.social, head of Biotechnology & Genome Editing, at @leibnizipk.bsky.social joins our webinar on New Genomic Techniques to boost sustainability in Europe”! 🇪🇺🌾

Join us Sep 18 at 16:00 CEST! 📅
Free Registrations at forms.office.com/e/RXJwce0Y4G
Informative card with speaker name and event title and date.