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Filip Van Petegem @filipvanpetegem.bsky.social · 1d

Very interesting paper from Susan Hamilton's lab, showing that phosphorylation of RyR1 by SPEG kinase can suppress pathological consequences of disease mutations.
www.science.org/doi/10.1126/...

Phosphorylation of RYR1 at Ser2902 decreases Ca2+ leak in skeletal muscle and susceptibility to malignant hyperthermia and heat stroke

Reducing Ca2+ leak through mutant RYR1 prevents pathological heat production in skeletal muscle.

www.science.org

Filip Van Petegem @filipvanpetegem.bsky.social · 5d

Our cryo-EM structure of the intermediate state of the KCNQ1 potassium channel is out. Collaborative effort with the David Fedida and Luca Maragliano labs. Cryo-EM work spearheaded by Efthimios Kyriakis, PhD

www.nature.com/articles/s41...

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Reposted by Filip Van Petegem

Misha Kudryashev @mishakudryashev.bsky.social · 16d

We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.

We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography

zenodo.org/records/1717...

Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles

zenodo.org

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Filip Van Petegem @filipvanpetegem.bsky.social · 18d

Hope this isn't going to apply to anyone already on H-1B, but only future ones.

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Filip Van Petegem @filipvanpetegem.bsky.social · Jun 1

www.science.org/doi/10.1126/...

A study in collaboration with Frank Bosmans. Reproducible protocol to measure the elusive NaV1.9 currents, and revealing a big role for the preIQ region in gating properties.

A robust expression system reveals distinct gating mechanisms and calmodulin regulation of NaV1.9 channels

An expression system enables detailed study of NaV1.9 channel gating and its regulation by calmodulin.

www.science.org

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Filip Van Petegem @filipvanpetegem.bsky.social · Mar 30

unconscious bias...

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Filip Van Petegem @filipvanpetegem.bsky.social · Mar 11

Still a few days left to apply for a faculty position focused on cardiac arrhythmias at Simon Fraser University. Come join the greater Vancouver network on arrhythmia research!
www.academicwork.ca/jobs/assista...

Assistant or Associate Professor in Cardiac Arrhythmias - CAUT | Academic Work

www.academicwork.ca

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Filip Van Petegem @filipvanpetegem.bsky.social · Mar 2

seems you can definitely forget about BLAST with an entire RyR sequence...

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Filip Van Petegem @filipvanpetegem.bsky.social · Mar 2

hopefully not a symptom of funding cuts and resignations at NIH

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Filip Van Petegem @filipvanpetegem.bsky.social · Mar 1

Is it just me or is Pubmed down...??

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Reposted by Filip Van Petegem

Oli Clarke @olibclarke.bsky.social · Feb 19

Well done Kookjoo Kim on a great poster presentation, here visited by an RyR VIP, @filipvanpetegem.bsky.social!

And thanks @biophysicalsoc.bsky.social, @popstarlab.bsky.social & team for a great #BPS2025 under challenging circumstances - see you next year in SF!

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Filip Van Petegem @filipvanpetegem.bsky.social · Nov 29

Stress in action: our crystal structure of PKA bound to its preferred substrate in CaV1.2 (Ser1981 for the aficionados). Quantitative experiments on previously proposed sites in CaV1.2 and its regulator Rad shows that there are two tiers of substrates.

jbc.org/article/S002...

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Filip Van Petegem @filipvanpetegem.bsky.social · Nov 29

an invitation to talk on the Galapagos Islands?

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Filip Van Petegem @filipvanpetegem.bsky.social · Nov 25

seconding this. It also applies to presentations. The perfect way to throw off your audience is to use highly specific jargon and acronyms to an audience that is not (fully) in your field

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Filip Van Petegem @filipvanpetegem.bsky.social · Nov 23

Calcins are scorpion-derived peptides that can cross the plasma membrane and modulate the Ryanodine Receptor. Marvin presenting the latest cryoEM results from our lab at the 2024 Translational Arrhythmia Day (TADA) meeting.
(description also here: www.science.org/doi/10.1126/...)

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Filip Van Petegem @filipvanpetegem.bsky.social · Nov 13

#CryoEM

Filip Van Petegem @filipvanpetegem.bsky.social · Nov 11

Every day since the late 1920s (except during WWII), the notes of the Last Post sound through the Menin Gate in Ypres, Belgium. Unfortunately, "never again" hasn't stuck. #ArmisticeDay

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Filip Van Petegem @filipvanpetegem.bsky.social · Nov 9

Can you find out which ion binds your protein using cryo-EM? This new method from Bonnie Murphy's lab combines electron energy loss spectroscopy and single particle imaging, showing this can become posssible. Test case on our favorite protein, the Ryanodine Receptor.

www.nature.com/articles/s41...

Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis - Nature Methods

An approach combining electron energy-loss spectroscopy with image processing tools from single-particle cryo-electron microscopy enables elemental mapping in macromolecular complexes, paving the way ...

www.nature.com

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Filip Van Petegem @filipvanpetegem.bsky.social · Oct 5

Our latest cryo-EM study, elucidating the type 3 Ryanodine Receptor (RyR3) in different conformational states
nature.com/articles/s4146…

Unexpected binding sites for ATP and chloride in the N-terminal region, affected by mutations linked to epileptic encephalopathy.

https://nature.com/articles/s4146…

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Filip Van Petegem @filipvanpetegem.bsky.social · Apr 17

A new faculty position in cardiac arrhythmia in the greater Vancouver area.

sfu.ca/content/dam/...

Come join our local arrhythmia community.

Reposted by Filip Van Petegem

HRMEM @hrmem.bsky.social · Jan 23

OK this is COOL i love it
www.biorxiv.org/content/10.1...

Cryo-EM analysis on magnetic beads for scarce macromolecules in heterogeneous samples

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

www.biorxiv.org

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Filip Van Petegem @filipvanpetegem.bsky.social · Jan 21

the method has been applied to worm haemoglobin and to one of our favorite proteins, the Ryanodine Receptor.

Filip Van Petegem @filipvanpetegem.bsky.social · Jan 21

A very cool method by Bonnie Murphy's lab to map elements for cryo-EM. The method, named REEL-EM, makes use of electron-energy loss spectroscopy. Honored to have contributed to this: www.biorxiv.org/content/10.1...

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Filip Van Petegem @filipvanpetegem.bsky.social · Nov 25

yeah, why Australia and not Canada..?

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