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HRMEM @hrmem.bsky.social · 2d

We are excited to share the recent paper from @filipvanpetegem.bsky.social featuring cryo-EM structures of the KCNQ1 potassium channel collected at HRMEM!
#cryoEM #structuralbiology
www.nature.com/articles/s41...

www.nature.com

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Reposted by HRMEM

Filip Van Petegem @filipvanpetegem.bsky.social · 5d

Our cryo-EM structure of the intermediate state of the KCNQ1 potassium channel is out. Collaborative effort with the David Fedida and Luca Maragliano labs. Cryo-EM work spearheaded by Efthimios Kyriakis, PhD

www.nature.com/articles/s41...

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HRMEM @hrmem.bsky.social · Jul 4

HRMEM is excited to share this new paper from the Cullis lab! Congrats to Miffy Cheng et al!
www.nature.com/articles/s41...

Liposomal lipid nanoparticles for extrahepatic delivery of mRNA - Nature Communications

Long-circulating, transfection-competent LNP-mRNA systems are key for effective extrahepatic delivery. Here, authors show that LNPs with high bilayer lipid ratios yield high mRNA encapsulation, prolon...

www.nature.com

HRMEM @hrmem.bsky.social · Jun 24

HRMEM is excited to spotlight this excellent review of cell envelope diversity and evolution from HRMEM cryoET user Eltiza Tocheva (@elitzatocheva.bsky.social‬) and team.

www.nature.com/articles/s41...

Cell envelope diversity and evolution across the bacterial tree of life - Nature Microbiology

This Review discusses the diversity of cell envelope architectures across major bacterial phyla and highlights conserved features found in diderm lineages.

www.nature.com

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HRMEM @hrmem.bsky.social · Jun 9

HRMEM is happy to spotlight this exciting work, which applied a magnetic separation technique to characterize LNP bimolecular coronas.

Congratulations to Valentina Francia, Yao Zhang and colleagues from the Cullis lab at UBC.

We are proud to have supported this work! 🔬

doi.org/10.1073/pnas...

A magnetic separation method for isolating and characterizing the biomolecular corona of lipid nanoparticles | PNAS

Lipid nanoparticle (LNP) formulations are a proven method for the delivery of nucleic acids for gene therapy as exemplified by the worldwide rollou...

www.pnas.org

Reposted by HRMEM

SBGrid Consortium @sbgrid.bsky.social · Jun 2

In case you missed our May webinar, it is now available!! Ellen Zhong from Princeton University presented CryoDRGN-AI.

Topic: CryoDRGN-AI: Neural ab initio reconstruction of challenging cryo-EM and cryo-ET datasets
Host: Pete Meyer
Recorded on: May 20, 2025

buff.ly/jKjWTOa

#SBGridWebinars

CryoDRGN

SBGrid webinars are hosted with partial support from the NIH R25 Continuing Education for Structural Biology Mentors #GM151273, in collaboration with Co-PI J...

youtu.be

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HRMEM @hrmem.bsky.social · May 26

#structuralbiology
#drugdiscovery #cryoEM #STEM #pharmacology #UBC

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HRMEM @hrmem.bsky.social · May 26

HRMEM is proud to spotlight this structure of the cardiac potassium channel, KCNQ1, bound to its activator, ML277!

Shout to Katrien Willegems, Jodene Eldstrom, Efthimios Kyriakis, Fariba Ataei and researchers from the Fedida and Van Petegem Labs at UBC! 🔬🫀🧬

#cryoEM

www.nature.com/articles/s41...

Structural and electrophysiological basis for the modulation of KCNQ1 channel currents by ML277 - Nature Communications

KCNQ1 channels are active in heart, brain and gut. Functional loss causes epilepsy and sudden arrhythmic death. Here, authors describe a key activator drug binding site, explaining isoform and drug se...

www.nature.com

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HRMEM @hrmem.bsky.social · May 14

#cryo-EM #STEM

HRMEM @hrmem.bsky.social · May 14

HRMEM is happy to spotlight this structure of calcineurin bound to PI4KA determined by Alexandria Shaw and colleagues from the Burke Lab at the University of Victoria and the Yip Lab at UBC!

We are proud to have provided the electron microscopy for this work!

Read more:
doi.org/10.1016/j.st...

Structure of calcineurin bound to PI4KA reveals dual interface in both PI4KA and FAM126A

Phosphatidylinositol 4-kinase alpha (PI4KA) maintains the phosphatidylinositol 4-phosphate (PI4P) and phosphatidylserine pools of the plasma membrane.…

www.sciencedirect.com

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Reposted by HRMEM

Morgridge Institute for Research @morgridgeinstitute.bsky.social · May 14

Mass Spec + lasers + cryoEM = COOL SCIENCE!

New research from @coonlab.bsky.social and the Grant Lab combines mass spec to land proteins on TEM grids, a laser to restore protein structure, and cryoEM imaging and analysis to produce 3D reconstructions.

www.mcponline.org/article/S153...

Laser-Induced Rehydration of Cryo-Landed Proteins Restores Native Structure

The use of native mass spectrometry (MS) to land biological molecules for subsequent cryogenic electron microscopy (cryoEM) imaging and three-dimensional reconstruction has gained momentum in recent y...

www.mcponline.org

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Reposted by HRMEM

Philippe Van der Stappen @phaips.vd.st · May 6

Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!

tomoguide.github.io

Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪

Welcome to TomoGuide

A step-by-step Cryo-ET guide

tomoguide.github.io

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HRMEM @hrmem.bsky.social · May 6

We are happy to spotlight these structures of human ABCA4, a crucial transporter for ocular function, determined by Jéssica Fernandes Scortecci and colleagues from the Molday and Van Petegem Labs at UBC! 🔬

We are happy to have supported this work! #cryoEM

Read more:
www.nature.com/articles/s41...

Cryo-EM structures of the ABCA4 importer reveal mechanisms underlying substrate binding and Stargardt disease - Nature Communications

ABCA4 is an ATP-binding cassette (ABC) transporter that flips N-retinylidenephosphatidylethanolamine (N-Ret-PE) to the cytoplasmic leaflet of photoreceptor membranes. ABCA4 mutations are associated wi...

www.nature.com

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HRMEM @hrmem.bsky.social · May 2

Cryo-ET of Thermotoga maritima in a study led by Danielle Sexton and colleagues from the Tocheva Lab at UBC broadened our understanding of outer membrane biogenesis!

Shout out to @elitzatocheva.bsky.social !

Read more about this awesome work supported by HRMEM here:

www.pnas.org/doi/10.1073/...

The cell envelope of Thermotogae suggests a mechanism for outer membrane biogenesis | PNAS

The presence of a cell membrane is one of the major structural components defining life. Recent phylogenomic analyses have supported the hypothesis...

www.pnas.org

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Reposted by HRMEM

Danielle Grotjahn @nanigrotjahn.bsky.social · May 1

🚨New preprint!🚨 #teamtomo
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.

Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science

bioRxivpreprint @biorxivpreprint.bsky.social · May 1

Surface morphometrics reveals local membrane thickness variation in organellar subcompartments https://www.biorxiv.org/content/10.1101/2025.04.30.651574v1

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HRMEM @hrmem.bsky.social · Apr 28

Bin Zhao and colleagues from the Cullis Lab at UBC have developed a technique to determine the pH inside LNPs using a pH-sensitive fluorescent dye-based DNA probe. 🧬🔬

HRMEM is happy to have supported the electron microscopy for this exciting research!

Read more :

doi.org/10.1016/j.bi...

Determination of the interior pH of lipid nanoparticles using a pH-sensitive fluorescent dye-based DNA probe

Lipid nanoparticles (LNPs) containing ionizable cationic lipids are proven delivery systems for therapeutic nucleic acids, such as small interfering R…

www.sciencedirect.com

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Reposted by HRMEM

Berkeley Lab Press @berkeleylabpress.bsky.social · Apr 22

"Not only will it provide sharper details of proteins and other molecules imaged with cryo-EM," says
@berkeleylab.lbl.gov 's Peter Denes, "but it should enable microscopists to image even proteins roughly half the size of what is currently possible."

Ultracool microscopy yields a sharper look at proteins

After years of frustration, researchers learn how to sharpen molecular images with liquid helium

www.science.org

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HRMEM @hrmem.bsky.social · Apr 22

We are excited to spotlight this structure of the type 3 ryanodine receptor determined by Yu Seby Chen and colleagues from UBC and the University of Siena!

It was a pleasure to collaborate with the Van Petegem (@filipvanpetegem.bsky.social) Lab on this lovely work!

www.nature.com/articles/s41...

Cryo-EM investigation of ryanodine receptor type 3 - Nature Communications

Ryanodine Receptor type 3 mediates ER Ca2+ release in different cell types. Here, the authors use cryo-EM to reveal a binding site for chloride and two distinct sites for ATP. Epileptic encephalopathy...

www.nature.com

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HRMEM @hrmem.bsky.social · Apr 17

HRMEM is happy to have supported this structure determination of Rab3GAP by Gage Fairlie, Kha Nguyen and colleagues.

Congratulations to the Burke Lab (@jburkevic.bsky.social )at the University of Victoria and the Yip Lab at UBC! 🔬🎉

Read more here:
www.nature.com/articles/s41...

Biochemical and structural characterization of Rab3GAP reveals insights into Rab18 nucleotide exchange activity - Nature Communications

Rab3GAP, a guanine nucleotide exchange factor for Rab18, regulates membrane trafficking and lipid droplet metabolism. The authors elucidated its molecular structure, mapped its potential substrate bin...

www.nature.com

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HRMEM @hrmem.bsky.social · Apr 12

Congratulations to Franco Li, Shaun Peters and colleagues from the Strynadka Lab at UBC for their recent publication showcasing cryo-EM structures of S. aureus TarGH in the presence of targocil-II!

HRMEM is proud to have supported this work! 🔬🫶

www.nature.com/articles/s41...

Cryo-EM analyses unveil details of mechanism and targocil-II mediated inhibition of S. aureus WTA transporter TarGH - Nature Communications

Cryo-EM illuminates atomic binding details and far-ranging conformational effects of the inhibitor targocil-II on wall teichoic acid ABC transporter TarGH, a virulence target from the antibiotic resis...

www.nature.com

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HRMEM @hrmem.bsky.social · Mar 4

People always ask me what resolution they need and I say...depends on the question you're asking. This is a really cool example of that extra resolution being meaningful!

Dominik Hrebik @dhrebik.bsky.social · Mar 3

I am very happy to announce that we solved the in-virus #cryoEM structure of the #HIV matrix protein. The structure helped us to resolve a long mystery in HIV biology. A🧵1/5
Read more in Nature: www.nature.com/articles/s41... 🧪

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Reposted by HRMEM

John Rubinstein @johnrubinstein.bsky.social · Feb 19

In @justinditrani.bsky.social final postdoc paper (with contributions from many others) we show that forming/purifying vesicles (here from M. smegmatis) allows structure det'n of *endogenous* membrane proteins in their *native lipid bilayer*
🧵 on methods & TB biology...
www.pnas.org/doi/full/10....

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HRMEM @hrmem.bsky.social · Jan 2

I find the lids that screw off completely WAY more staticky than the pin lids with the little door, FWIW

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HRMEM @hrmem.bsky.social · Dec 24

Sure does :) these are homemade so not sure of the exact thickness!

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HRMEM @hrmem.bsky.social · Sep 6

Millin' like a villain

Image of a lamella created in a FIB-SEM instrument. The image shows the ion beam image with the lamella appearing as a narrow head on strip with trenches either side.

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