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giorgioseano.bsky.social
Giorgio Seano
@giorgioseano.bsky.social
1.5K followers 920 following 100 posts
Head of the Tumor Microenvironment Laboratory at Institut Curie. #CancerResearch, #Neuro-oncology, #BrainTumor, #GBM, #Glioblastoma, #ResistanceMechanism, #TumorMicroenrironment, #TME, #Mechanobiology, #CellPlasticity https://institut-curie.org/team/seano
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Giorgio Seano @giorgioseano.bsky.social · Jul 17
🧠 Why does glioblastoma always outsmart treatment? In our #Neuro-Oncology paper, we identified proneural-mesenchymal hybrid glioblastoma cells that are resistant to therapy and dependent on nuclear import. doi.org/10.1093/neuo...
Short walkthrough below. Let’s dive in! 🧵 (1/9)
#GBM, #BrainTumor
Background: Despite extensive research efforts, glioblastoma (GBM) remains a deadly disease with poor prognosis. Although previous studies have identified various cell states within GBM tumors, the molecular mechanism underlying adaptive GBM cell plasticity induced by conventional therapy remains unclear. Methods: We used fluorescent reporters for proneural (PN) and mesenchymal (MES) subtypes to monitor GBM cell plasticity in real-time across multiple patient-derived cell lines. This approach revealed cells that concurrently expressed both proneural and mesenchymal markers. To investigate this unique hybrid population, we implemented a comprehensive methodological approach encompassing bulk and single-cell RNA sequencing, single-cell ChIP sequencing, nuclear proteomics, high-resolution imaging, orthotopic mouse models, clinical dataset analysis, and pharmacological and genetic techniques. This multifaceted strategy allowed us to gain functional and molecular insights into this distinct cellular population. Results: We showed that these hybrid cells are increased by conventional therapies, and are resistant to these therapies. At the molecular level, hybrid cells display significant alterations in chromatin structure and nuclear protein composition, elevated transcriptional activity, Myc activation, and improved transport between the nucleus and cytoplasm. Genetic and pharmaceutical inhibition of the nuclear import/export shuttling machinery, increased in hybrid cells, effectively suppressed adaptive GBM cell plasticity and hybrid identity, thereby enhancing the sensitivity of GBM cells to therapies. Conclusion: Our results indicate that GBM hybrid cells play a crucial role in chemoradiation resistance. The nuclear transport machinery presents a potential therapeutic target for hybrid cells, offering a way to counteract the typical resistance to treatment observed in GBM.
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Reposted by Giorgio Seano
sunrise-network.bsky.social
SUNRiSE network @sunrise-network.bsky.social · Jul 18
Amazing job! Well done guys! Funny how conventional therapies enrich for hybrid GBM cells🤩
giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
🧠 Why does glioblastoma always outsmart treatment? In our #Neuro-Oncology paper, we identified proneural-mesenchymal hybrid glioblastoma cells that are resistant to therapy and dependent on nuclear import. doi.org/10.1093/neuo...
Short walkthrough below. Let’s dive in! 🧵 (1/9)
#GBM, #BrainTumor
Background: Despite extensive research efforts, glioblastoma (GBM) remains a deadly disease with poor prognosis. Although previous studies have identified various cell states within GBM tumors, the molecular mechanism underlying adaptive GBM cell plasticity induced by conventional therapy remains unclear. Methods: We used fluorescent reporters for proneural (PN) and mesenchymal (MES) subtypes to monitor GBM cell plasticity in real-time across multiple patient-derived cell lines. This approach revealed cells that concurrently expressed both proneural and mesenchymal markers. To investigate this unique hybrid population, we implemented a comprehensive methodological approach encompassing bulk and single-cell RNA sequencing, single-cell ChIP sequencing, nuclear proteomics, high-resolution imaging, orthotopic mouse models, clinical dataset analysis, and pharmacological and genetic techniques. This multifaceted strategy allowed us to gain functional and molecular insights into this distinct cellular population. Results: We showed that these hybrid cells are increased by conventional therapies, and are resistant to these therapies. At the molecular level, hybrid cells display significant alterations in chromatin structure and nuclear protein composition, elevated transcriptional activity, Myc activation, and improved transport between the nucleus and cytoplasm. Genetic and pharmaceutical inhibition of the nuclear import/export shuttling machinery, increased in hybrid cells, effectively suppressed adaptive GBM cell plasticity and hybrid identity, thereby enhancing the sensitivity of GBM cells to therapies. Conclusion: Our results indicate that GBM hybrid cells play a crucial role in chemoradiation resistance. The nuclear transport machinery presents a potential therapeutic target for hybrid cells, offering a way to counteract the typical resistance to treatment observed in GBM.
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Reposted by Giorgio Seano
oncodaily.bsky.social
OncoDaily @oncodaily.bsky.social · Jul 18
Why Does Glioblastoma Always Outsmart Treatment? - Giorgio Seano
@giorgioseano.bsky.social
@institutcurie.bsky.social
@valentino-ribecco.bsky.social
@ggargiul.bsky.social

oncodaily.com/science/glio...

#OncoDaily #Oncology #Cancer #Health #Medicine #MedEd #MedOnc #MedNews #Glioblastoma #NeuroOnc
Giorgio Seano: Why Does Glioblastoma Always Outsmart Treatment? - OncoDaily
Giorgio Seano: Why Does Glioblastoma Always Outsmart Treatment? / Adrien Thomas, Alberto Ballestín, cancer, Cathy Pichol-Thievend, Celine Vallot, Curie
oncodaily.com
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
You can dive into all the multi-omics data and findings in the full paper.
Read more: doi.org/10.1093/neuo....
🧵 (9/9)
Proneural-Mesenchymal hybrid glioblastoma cells are resistant to therapy and dependent on nuclear import
AbstractBackground. Despite extensive research efforts, glioblastoma (GBM) remains a deadly disease with poor prognosis. Although previous studies have ide
doi.org
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
🌟 Special thanks to @eanoassociation.bsky.social for sharing our work, and heartfelt gratitude to the @erc.europa.eu, @fondationarc.bsky.social @institutcurie.bsky.social and @cnrsbiologie.bsky.social for their generous support. (8/9)
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
🙏 This research wouldn’t have been possible w/o the work of Oceane Anezo, Jeremy Raymond, Alberto Ballestín, Cathy Pichol-Thievend, Juliette Reveilles, Adrien Thomas, @valentino-ribecco.bsky.social, and the collaboration with Celine Vallot, @ggargiul.bsky.social , Vidhya M. Ravi, Kevin Joseph (7/9)
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
🎉 Huge congrats to Guillaume Bourmeau, leading author and my brilliant PhD student, for spearheading this work on GBM hybrid cells at @institutcurie.bsky.social! Your dedication made this breakthrough possible. (6/9)
#ProudPI,
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
🚀 Here's the exciting part: we can target this vulnerability.
We hit them with importazole (import blocker) and selinexor (export inhibitor).
🔥 Targeting nuclear import decreases the hybrid cell pool and enhances chemoradiation sensitivity, opening a new therapeutic avenue against GBM. (5/9)
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
🛡️ Therefore, these cells are resistant to treatment and are linked to a poorer patient prognosis. Using RNA-seq, nuclear proteome and scChIP-Seq, we discovered their Achilles' heel: a hyperactive nuclear transport system they rely on to import oncogenes like MYC and maintain their state. (4/9)
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
🤯 These hybrid cells don’t just look different, they proliferate like they’re on Red Bull and shrug off DNA damage after irradiation. Have they got superhuman repair skills? 🙄 (3/9)
a cartoon drawing of a person holding a pencil with a lightning bolt around it
ALT: a cartoon drawing of a person holding a pencil with a lightning bolt around it
media.tenor.com
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
🔬 Using dual fluorescent reporters for proneural & mesenchymal states, we tracked live patient-derived glioblastoma lines and uncovered a hybrid population expressing both signatures... let's talk about an identity crisis! (2/9)
a man is peeking through a doorway and smiling .
ALT: a man is peeking through a doorway and smiling .
media.tenor.com
1 1
giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jul 17
🧠 Why does glioblastoma always outsmart treatment? In our #Neuro-Oncology paper, we identified proneural-mesenchymal hybrid glioblastoma cells that are resistant to therapy and dependent on nuclear import. doi.org/10.1093/neuo...
Short walkthrough below. Let’s dive in! 🧵 (1/9)
#GBM, #BrainTumor
Background: Despite extensive research efforts, glioblastoma (GBM) remains a deadly disease with poor prognosis. Although previous studies have identified various cell states within GBM tumors, the molecular mechanism underlying adaptive GBM cell plasticity induced by conventional therapy remains unclear. Methods: We used fluorescent reporters for proneural (PN) and mesenchymal (MES) subtypes to monitor GBM cell plasticity in real-time across multiple patient-derived cell lines. This approach revealed cells that concurrently expressed both proneural and mesenchymal markers. To investigate this unique hybrid population, we implemented a comprehensive methodological approach encompassing bulk and single-cell RNA sequencing, single-cell ChIP sequencing, nuclear proteomics, high-resolution imaging, orthotopic mouse models, clinical dataset analysis, and pharmacological and genetic techniques. This multifaceted strategy allowed us to gain functional and molecular insights into this distinct cellular population. Results: We showed that these hybrid cells are increased by conventional therapies, and are resistant to these therapies. At the molecular level, hybrid cells display significant alterations in chromatin structure and nuclear protein composition, elevated transcriptional activity, Myc activation, and improved transport between the nucleus and cytoplasm. Genetic and pharmaceutical inhibition of the nuclear import/export shuttling machinery, increased in hybrid cells, effectively suppressed adaptive GBM cell plasticity and hybrid identity, thereby enhancing the sensitivity of GBM cells to therapies. Conclusion: Our results indicate that GBM hybrid cells play a crucial role in chemoradiation resistance. The nuclear transport machinery presents a potential therapeutic target for hybrid cells, offering a way to counteract the typical resistance to treatment observed in GBM.
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Reposted by Giorgio Seano
annaclemens.com
Anna Clemens, PhD @annaclemens.com · Jul 13
🎧 I made this list in 2021, but these podcasts are still in my rotation!

If you're a researcher looking for relevant and useful information for academics, check out these top podcast suggestions and learn something new this week. -> https://rpst.page.link/rxCs

#AcademicWriting #PhDSky #PhDchat
Academic Podcasts for Researchers & Scientists
Looking for the best academic podcasts? I have recommendations for academic writing podcasts, podcasts for PhD students and other podcasts on academia – from leadership and academic life to teaching.
rpst.page.link
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Reposted by Giorgio Seano
eanoassociation.bsky.social
EANO @eanoassociation.bsky.social · Jun 18
📣 Have you heard? Registration for #EANO2025, taking place Oct 16-19, 2025 in #Prague (CZ) is open! Don’t miss out on this fantastic experience to meet experts in the field of #NeuroOncology. Early Bird fees available until July 31, 2025. 👉 eano.eu/eano2025/reg... #braincancer #medsky
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Reposted by Giorgio Seano
elizsmckenna.bsky.social
Elizabeth McKenna @elizsmckenna.bsky.social · Apr 27
Congratulations to the 2025 Cancer Discovery Early Career Award winner: Sandra Misale! @sandramisale.bsky.social #AACR25
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Apr 7
Well deserved!! Congrats Dr. Jain!!
theaacr.bsky.social
American Association for Cancer Research (AACR) @theaacr.bsky.social · Apr 3
The AACR congratulates Rakesh K. Jain, PhD, FAACR, who will be honored with the 2025 AACR Award for Lifetime Achievement in Cancer Research at #AACR25. www.aacr.org/about-the-aa... #AACRFellows @harvardmed.bsky.social
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Reposted by Giorgio Seano
theaacr.bsky.social
American Association for Cancer Research (AACR) @theaacr.bsky.social · Apr 3
The AACR congratulates Rakesh K. Jain, PhD, FAACR, who will be honored with the 2025 AACR Award for Lifetime Achievement in Cancer Research at #AACR25. www.aacr.org/about-the-aa... #AACRFellows @harvardmed.bsky.social
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Reposted by Giorgio Seano
eanoassociation.bsky.social
EANO @eanoassociation.bsky.social · Apr 3
📢 Call for Applications
EANO & @neuroonc.bsky.social are seeking a new Editor-in-Chief for Neuro-Oncology Advances!

✅ Strong background in #NeuroOncology
✅ Editorial leadership experience

🗓️ Deadline: May 31, 2025
🔗 More info: bit.ly/4j9AqV7

#EANO #SNO #NeuroOncologyAdvances #CallForApplications
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Reposted by Giorgio Seano
biorxiv-cancer.bsky.social
bioRxiv Cancer Bio @biorxiv-cancer.bsky.social · Mar 4
Methylation-Based Deconvolution Unveils Glioblastoma Heterogeneity and Cell-Type Composition Linked to Patient Survival https://www.biorxiv.org/content/10.1101/2025.02.27.640603v1
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Feb 17
Congrats @thomasdaubon.bsky.social... Cool story!!
thomasdaubon.bsky.social
Thomas Daubon pro - GBmetabo @thomasdaubon.bsky.social · Feb 4
1/5 Excited to share our latest paper published in EMBO Molecular Medicine!🎉 🎉 embopress.org/doi/full/10....
This collaborative work brings together Team1 BRIC_Bordeaux, @ahmadsharanek.bsky.social, Andreas Bikfalvi, Lucie Brisson, Thomas Mathivet and our GBmetabo Team with Audrey Burban
Exploiting metabolic vulnerability in glioblastoma using a brain-penetrant drug with a safe profile | EMBO Molecular Medicine
imageimageIn patient-derived and animal tumour models, mubritinib suppressed glioblastoma stem cells and tumour growth by targeting mitochondrial respiration. Combination with the current standard of ...
embopress.org
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Feb 16
Of course! Done…
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Reposted by Giorgio Seano
erictopol.bsky.social
Eric Topol @erictopol.bsky.social · Feb 3
A new paper on microplastics accumulating in the brain was just published in Nature Medicine. I've reviewed the background and main findings in a new Ground Truths post erictopol.substack.com/p/the-microp...
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jan 30
Looking forward to meeting a lot of good international and French friends at the Brain Tumor Microenvironment Symposium organized by the Institut du Cerveau... Super cool lineup!! #GBM #TME
curamus-cancer.fr/brain-tumor-...
SAVE THE DATE: Brain Tumor Microenvironment Symposium, 30th & 31st January 2025, Paris - Siric Cancer - Curamus - Site de Recherche Intégrée sur le Cancer
Dans le cadre de son programme de recherche intégrée « Cerveau, cancer et immunité – dialogue cellulaire dans les tumeurs cérébrales », […]
curamus-cancer.fr
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Reposted by Giorgio Seano
profharley.bsky.social
Brendan Harley @profharley.bsky.social · Jan 21
Wanted to stat today with some new #harleylab science.

ChiaWen Chang is leading efforts to understand immune cell patterning in the brain in glioblastoma. He started w/ the inverse question: how do GBM cells influence microglia (brain-resident immune cells)?

www.sciencedirect.com/science/arti...
Glioblastoma drives protease-independent extracellular matrix invasion of microglia
Glioblastoma (GBM) is the most common and lethal form of primary brain cancer. Microglia infiltration into the tumor microenvironment is associated wi…
www.sciencedirect.com
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jan 14
Sure, done!!
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giorgioseano.bsky.social
Giorgio Seano @giorgioseano.bsky.social · Jan 10
Sure!! You’re in!!
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