Gade et al. report that polysomes and mRNA levels impact the biophysical properties of the cytoplasm. Using passive rheology probes and single-particle tracking, they demonstrate that intracellular diffusion is enhanced when polysomes are disassembled or when mRNA levels are reduced in eukaryotic cells.

Cell-free translation is a powerful tool for studying protein synthesis. Here, we present a protocol for monitoring mRNA translation and degradation u…

Nature Biotechnology - A deep convolutional neural network model predicts the influence of the full-length mRNA sequence on translation efficiency.

Matched ribosome profiling and RNA sequencing data quantify translation efficiency patterns across 140 human and mouse cell lines.

Intracellular pathogens hijack host gene expression to subvert cellular processes. Here, the authors show that Legionella pneumophila’s PieF effector inhibits the human CCR4-NOT deadenylation machiner...

Helicobacter pylori CagA protein strongly inhibits functional and pathogenic amyloid fibril formation in both bacteria and humans.

Bäumlin et al. investigate the roles of coronavirus Nsp1 in translation inhibition and mRNA degradation using a human cell-free translation system. They demonstrate that SARS-CoV-2 Nsp1 mediates mRNA degradation independently of translation, whereas MERS-CoV Nsp1 inhibits translation without degradation, revealing distinct mechanisms and co-evolutionary protection of viral mRNAs.

Bäumlin et al. investigate the roles of coronavirus Nsp1 in translation inhibition and mRNA degradation using a human cell-free translation system. They demonstrate that SARS-CoV-2 Nsp1 mediates mRNA ...