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The Matić Lab

@maticlab.bsky.social

92 followers 76 following 15 posts

We are interested in ADP-ribosylation, PARP1 signalling, proteomics, histone PTMs, protein engineering, the DNA damage response and aging Max Planck Institute for Biology of Ageing, Cologne, Germany

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The Matić Lab @maticlab.bsky.social · Jul 11

At its core, it’s a technological paper—necessary to overcome some of the many challenges of working with ADPrUb. But beyond the technical details, below are the main highlights:

The Matić Lab @maticlab.bsky.social · Jul 9

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

Reposted by The Matić Lab

MPI for Biology of Ageing @mpiage.bsky.social · 1d

We were delighted to have Nobel laureate Venki Ramakrishnan with us to discuss his exciting new book, "Why We Die." Thanks so much, Venki, for your fascinating and thought-provoking talk - it was truly a pleasure to have you join us!

Reposted by The Matić Lab

Cologne Graduate School of Ageing Research @cga-age.bsky.social · Aug 12

Apply now for a fully funded #PhD position at the Cologne Graduate School of Ageing Research and become part of our vibrant #AgingResearch commmuny.
🗓️ Deadline: November 3, 2025.
ℹ️ More info: www.ageing-grad-school.de
📣 Spread the news!

The Matić Lab @maticlab.bsky.social · 20d

Superb News&Views on our recent paper reporting identification of ADP-ribosyl-linked serine ubiquitylation
www.nature.com/articles/s41...

ADPr gets a ubiquitin upgrade - Nature Chemical Biology

MARUbylation is a hybrid post-translational protein modification in which mono-ADP-ribosylation acts as a scaffold for sequential mono- and polyubiquitylation. Recent studies illuminate its substrates...

The Matić Lab @maticlab.bsky.social · Jul 11

• Together with a series of papers and preprints published this year, it establishes RNF114 as a reader of ADPrUb

The Matić Lab @maticlab.bsky.social · Jul 11

• Observation that serine mono-ADPrUb increases in ARH3 KO cells, raising the intriguing question of its potential role in ARH3 mutation–associated neurodegeneration

The Matić Lab @maticlab.bsky.social · Jul 11

• First reagent specific for ADPrUb, enabling both immunoblotting and immunoprecipitation

The Matić Lab @maticlab.bsky.social · Jul 11

• First mass spectrometric evidence of ADPrUb, regardless of the target residue

The Matić Lab @maticlab.bsky.social · Jul 11

• Serine mono-ADPrUb as a new type of DNA damage-induced histone modification

The Matić Lab @maticlab.bsky.social · Jul 11

• First evidence that, in cells, serine mono-ADPr by the PARP1/HPF1 writer complex is targeted by this unconventional ubiquitylation

The Matić Lab @maticlab.bsky.social · Jul 11

At its core, it’s a technological paper—necessary to overcome some of the many challenges of working with ADPrUb. But beyond the technical details, below are the main highlights:

The Matić Lab @maticlab.bsky.social · Jul 9

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

Reposted by The Matić Lab

Nature Chemical Biology @natchembio.nature.com · Jul 10

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. A proteomics approach is developed using these domains to map ADP-ribosyl-ubiquitylation sites

www.nature.com/articles/s41...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

Reposted by The Matić Lab

Maria Dilia Palumbieri @mariapalumbieri.bsky.social · Jul 10

Thrilled to share my first first-author postdoc publication — a team effort with @aklvnbch.bsky.social. We present the first MS-based evidence of ADP-ribosylation–linked ubiquitin (ADPrUb) in cells after DNA damage.
Grateful for all I’ve learned and looking forward to uncovering more about ADPrUb

The Matić Lab @maticlab.bsky.social · Jul 9

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

Reposted by The Matić Lab

Andreas Kolvenbach @aklvnbch.bsky.social · Jul 9

Really happy that our paper is finally released. We show that PARP1 and histones are targets of ADP-ribosyl-linked ubiquitylation during DNA damage and describe how to detect the dual modification. Great teamwork with @mariapalumbieri.bsky.social & thanks to everyone involved @maticlab.bsky.social !

The Matić Lab @maticlab.bsky.social · Jul 9

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

The Matić Lab @maticlab.bsky.social · Jul 9

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

Reposted by The Matić Lab

MPI for Biology of Ageing @mpiage.bsky.social · Jun 27

Huge Congratulations to Edoardo José Longarini on receiving the prestigious Peter Hans Hofschneider Prize! 🎉

www.age.mpg.de/424030/Edoar...

Edoardo Longarini receives the Peter Hans Hofschneider Prize

Edoardo Longarini receives the prestigious Peter Hans Hofschneider Prize for his exceptional work in molecular medicine.

Reposted by The Matić Lab

Cologne Graduate School of Ageing Research @cga-age.bsky.social · Mar 20

🎥We're excited to share the new film about our graduate programme. Get a glimpse of our faculty & PhD students and explore your research opportunities. Join us as we dive into the world of ageing research and discover what makes the CGA truly unique.👩‍🔬🧑‍🎓 www.ageing-grad-school.de/about-us/cal...

Discover the CGA film

Get to know our people & programme

www.ageing-grad-school.de

Reposted by The Matić Lab

Anthony Leung @leunglab.bsky.social · Mar 13

New Paper💡PARP enzymes regulate ADP-ribosylation, a protein modification linked to cancer, infections & neurodegeneration. But it comes with two forms—MARylation (single) & PARylation (multiple). Meet dELTA-MS, a mass spec method that identifies both in one experiment!
pubs.acs.org/doi/full/10....

dELTA-MS: A Mass Spectrometry-Based Proteomics Approach for Identifying ADP-Ribosylation Sites and Forms

ADP-ribosylation, characterized by the addition of adenosine diphosphate ribose, can occur in both monomeric (MARylation) and polymeric (PARylation) forms. Little is known about the specific contribut...

Reposted by The Matić Lab

Mike Cohen @michaelnadbio.bsky.social · Feb 25

I'm super excited to share our study in collaboration with @jnpruneda.bsky.social, published today in @embojournal.org! We show that PARPs are modified with a non-canonical ester-linked mono-ADP-ribosylated ubiquitin species for the first time in cells: www.embopress.org/doi/full/10....

Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose | The EMBO Journal

imageimageCertain E3 ligases have been found to ubiquitylate hydroxyl groups on free NAD+ and ADP-ribose in vitro, but the in vivo occurrence of this dual post-translational modification has remained ...

www.embopress.org

The Matić Lab @maticlab.bsky.social · Feb 9

These new antibody development efforts align with our vision that the fundamental discovery of Serine ADP-ribosylation can be converted into a technology that transforms the study of ADP-ribosylation and related PTMs.

The Matić Lab @maticlab.bsky.social · Feb 9

This preprint is part of a larger manuscript on additional antibodies and their applications to biological questions. The goal of this preprint is to share these poly-ADPr with the community without delay, as we believe they wil benefit many laboratories.

The Matić Lab @maticlab.bsky.social · Feb 9

As with our other tools, the AbD64138 clone should become widely accessible in a few months. For those familiar with our popular AbD43647 antibody, the new AbD64138 clone provides similar advantages for poly-ADPr as AbD43647 does for mono-ADPr.

The Matić Lab @maticlab.bsky.social · Feb 9

We’re particularly excited to share with the community the exceptionally high sensitivity we’ve achieved for poly-ADPr immunoblotting, all while using a faster and simpler protocol enabled by a sophisticated SpyTag-HRP format that eliminates the need for secondary antibodies.

The Matić Lab @maticlab.bsky.social · Feb 9

We’ve uploaded a bioRxiv preprint on new antibodies specific for poly-ADP-ribosylation (PARylation). These modular tools were developed and validated through novel applications of our Serine ADP-ribosylation-based technology. www.biorxiv.org/content/10.1...

The Matić Lab @maticlab.bsky.social · Jan 29

Hi everyone! The Matić Lab is now on Bluesky. Looking forward to sharing our discoveries and technologies and interacting with all of you.