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Reposted by Maurice Lee

Reto Fiolka @retof.bsky.social · Feb 12

The self-driving multiscale microscope from @daetwylerstephan.bsky.social is out.

It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution.

We used it to study cancer-immune cell interactions.

www.nature.com/articles/s41...

Imaging of cellular dynamics from a whole organism to subcellular scale with self-driving, multiscale microscopy

Nature Methods - A self-driving multiresolution light-sheet microscope enables the simultaneous observation and quantification of cellular and subcellular dynamics in the context of intact and...

www.nature.com

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Maurice Lee @mauriceylee.bsky.social · Feb 3

Goodbye Mr Snouty. I had a lot of fun working with you. It has been a blast. Hopefully the next scientist/engineer who works with you will enjoy the process too.🥹

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Reposted by Maurice Lee

BWJones @bwjones.bsky.social · Jan 14

Science bio-imaging question:

What platform are people liking for confocal / super-resolution imaging these days? Leica? Zeiss? Olympus? Nikon? Other?

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Maurice Lee @mauriceylee.bsky.social · Jan 15

I like our Andor Dragonfly spinning disk confocal with two Sona sCMOS cameras. It's connected to a Nikon Eclipse Ti2 body. The powerful lasers, high NA Nikon objectives, and Nikon's PFS autofocus system allow us to easily do SMLM.

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Maurice Lee @mauriceylee.bsky.social · Jan 13

You can always Facebook message me, Dave!😆 I hope you and the family are doing well!

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Maurice Lee @mauriceylee.bsky.social · Jan 13

Hello science friends on bluesky!
After many years of doing molecular biophysics and optics/microscopy, I just want to share that I'm leaving science and academia next month. I don't have concrete plans for my next step yet so I might just take a break for a while but we shall see how things go.

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Reposted by Maurice Lee

Christophe 🔬 L @christlet.bsky.social · Nov 20

Also in today's preprint trawling: imaging at depth is difficult, so let's just... squish cells? arxiv.org/abs/2411.12656

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Maurice Lee @mauriceylee.bsky.social · Nov 21

Everything is 2D when you squish them enough!

Maurice Lee @mauriceylee.bsky.social · Nov 20

search.app?link=https%3...

Probe set selection for targeted spatial transcriptomics - Nature Methods

Spapros is a probe set selection pipeline for targeted spatial transcriptomics that optimizes for both transcriptional and within-cell type variation.

search.app

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Maurice Lee @mauriceylee.bsky.social · Nov 19

I think what I learned from staring at your picture is that in an infinitely large optical table with an infinite number of configurations, all the microscopes ever built by our finite minds exist, including those that have bad aberrations and those that have perma oil stains on them

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Reposted by Maurice Lee

Alex Donovan @apadonovan.bsky.social · Nov 18

Does anyone know any good publications/databases to find non-coding variant loci for neurodevelopmental disorders? (anything, but particularly disorders of brain size if possible)

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Reposted by Maurice Lee

Brian Beliveau @oligopain.bsky.social · Nov 15

The pSABER paper is now online!

pSABER is a new method for amplifying in situ hybridization signals

This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social

doi.org/10.1038/s415...

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Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER - Nature Methods

pSABER combines the power of signal amplification by exchange reaction (SABER) with the deposition of fluorescent or colorimetric substrates by horseradish peroxidase to enable enhanced signals for in...

doi.org

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Maurice Lee @mauriceylee.bsky.social · Nov 15

Caring for your friends in science means lending them your neutral density filter wheel to see if it will help to alleviate their microscope problems

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Maurice Lee @mauriceylee.bsky.social · Nov 14

please don't do this.
but i do have objectives that are broken and beyond repair. and i also do have immersion oil in the lab. and i also do have an optical table. hmmm

DrLachie @drlachie.bsky.social · Nov 13

So.....don't do this?

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Reposted by Maurice Lee

Amir Rahmani @astormic.bsky.social · Nov 14

Calling all super-resolution microscopy enthusiasts!

Join us in Leeds for an inspiring one-day event and hear the latest developments in #SuperResolutionMicroscopy from amazing invited speakers.

Don’t miss your chance to connect, learn, and share insights! Register & submit abstracts now!

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Maurice Lee @mauriceylee.bsky.social · Nov 14

Hello everyone! So many of you have followed me over the past several days. I'm guessing most are interested in my science research work (i.e., fluorescence microscopy, image analysis, spatial transcriptomics). So I decided to wind down my bird app account and slowly post a little more on Bluesky.

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Reposted by Maurice Lee

Beth Cimini 🔬💻📊 @bethcimini.bsky.social · Nov 6

Made a starter pack for microscopy and image analysis - please (self-)nominate additions!

go.bsky.app/Nwa91pC

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Reposted by Maurice Lee

Izzy Jayasinghe @ijayas.bsky.social · Nov 7

For folks just arriving in #AcademicBsky or #ScienceBsky, here are a couple of Biophysics starter packs. Biophysicists, pls tag yourself and others and I will add you to the new starter packs. go.bsky.app/4AUjqVz

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Maurice Lee @mauriceylee.bsky.social · Nov 9

I had the opportunity to conduct a hands-on workshop on basic optical alignment today!
Zesheng and Alex, from Dr Liang Kaicheng's group, joined me in the optics lab where we covered the basics such as "walking" the laser beam, collimating lasers, and checking back reflections!

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Maurice Lee @mauriceylee.bsky.social · Nov 1

The 21st Annual Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications hosted by the CRL Molecular Imaging Center at UC Berkeley on 23-25 Jan 2024.
www.eventbrite.com/e/21st-annua...

21st Annual Advanced Imaging Methods Workshop

The Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications.

www.eventbrite.com

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Maurice Lee @mauriceylee.bsky.social · Oct 4

I think these are my favorite forever stamps ever!🖤🤍♥️💚🩵🧡

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Maurice Lee @mauriceylee.bsky.social · Sep 19

AI powered microscope to spot cancer? Built by Google and DOD?
www.cnbc.com/2023/09/18/g...

Google and the Department of Defense are building an AI-powered microscope to help doctors spot canc...

The AI-powered tool is called an Augmented Reality Microscope, or ARM, and Google and the Department of Defense have been quietly working on it for years.

www.cnbc.com

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Maurice Lee @mauriceylee.bsky.social · Aug 31

Singapore's a blue zone city!?!?! I guess my life expectancy is ~81
finance.yahoo.com/news/longevi...

The longevity secrets of Singapore, the 6th blue zone city where people are living the longest, happ...

The five health and happiness secrets of Singapore, "blue zone 2.0—the next frontier of aging."

finance.yahoo.com

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Maurice Lee @mauriceylee.bsky.social · Aug 19

We have started to work on 3D spatial transcriptomics of the whole larval zebrafish brain and I'm excited to share an image that we recently acquired in the lab!
Every bright spot or blob represents the RNA molecules that we've labelled and imaged!
The image below is 1750*1050 microns.

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Maurice Lee @mauriceylee.bsky.social · Aug 16

"By combining single-cell sequencing with methods to map the spatial location of gene expression, scientists are unravelling the extraordinary cellular diversity of the brain."
https://www.nature.com/articles/d41586-023-02559-9

The quest to map the mouse brain

By combining single-cell sequencing with methods to map the spatial location of gene expression, scientists are unravelling the extraordinary cellular diversity of the brain. By combining single-cell ...

www.nature.com

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