In Arabidopsis, transcriptional factors LEC2 and SPCH enhance local auxin biosynthesis, establishing a transcriptional framework that enables differentiated single somatic epidermal cells to regain to...

DNA connects modern Slavs to a wave of migration following the fall of the Roman Empire

Advances in genome engineering have paved the way for targeted epigenome engineering, providing fundamental insights into the role of epigenetic modifications in trait inheritance. Engineered epialleles have already delivered stable, heritable changes in agronomic traits. Despite this capacity, progress in the field has not yet achieved its potential, leaving many avenues of research unexplored. In this review we examine the factors influencing this progress, including the advances in current epigenome editing techniques, the key research goals and translational applications, and the challenges in the selection of ideal target loci. We propose that improved tools for the selection of target loci, particularly in large and complex genomes, are needed to propel the field forward.

Chromatin is the complex of DNA, RNA and protein that is found making up the chromosomes in eukaryotic cells. Chromatin is essential for proper genome function and is involved in chromosome segregati…

3D reconstruction of Arabidopsis roots treated with a scale-based method and pre-stained. (A–C) 3D reconstruction of 70 serial optical sections of H2B::YFP and EdU-Eterneon-labeled cells in roots using a scale-based method. EdU-Eterneon labeling (red) marks nuclei undergoing DNA replication, while H2B::YFP (green) labels the entire nucleus. (D–F) 3D projection of roots SYTOX-stained and EdU. EdU-positive nuclei (red) marks nucleus with DNA replication and SYTOX Green (Green) marks nuclear acids. Scale bars = 20 μm.