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Reposted by Kazuhiro Maeshima

Graham Dellaire @docdellaire.bsky.social · 27d

3rd histone paper on my feed today!

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Mar 7

Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1...
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · 27d

Thanks. Yes, we agree to your point.

Kazuhiro Maeshima @kazu-maeshima.bsky.social · 27d

Thanks @zeinabrekad.bsky.social for your kind words. We totally agree to your point, and indeed we are working on H1:
bsky.app/profile/kazu...
www.biorxiv.org/content/10.1...

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · 27d

Thanks! 😃

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Reposted by Kazuhiro Maeshima

Masa A. Shimazoe @masaashimazoe.bsky.social · Aug 29

Condensed chromatin domain structure and cohesin-constrained mobility assure independent transcription regulation for each domain!
Also consistent with many previous studies.
(4/n)

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Reposted by Kazuhiro Maeshima

Masa A. Shimazoe @masaashimazoe.bsky.social · Aug 28

Euchromatin is mostly condensed⁉️

With @shiori-iida.bsky.social , We revealed the detailed structure of euchromatin using super-resolution imaging.

Cohesin regulates the function of euchromatin via chromatin dynamics!
(1/n)

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 28

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing.
www.biorxiv.org/cgi/content/...

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Reposted by Kazuhiro Maeshima

Shiori Iida @shiori-iida.bsky.social · Aug 29

One more movie!
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)

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Reposted by Kazuhiro Maeshima

Shiori Iida @shiori-iida.bsky.social · Aug 28

Our new preprint is out!
We combined single-nucleosome imaging and 3D-SIM to reveal:
🔹 Euchromatin forms condensed domains, not open fibers
🔹Cohesin loss increases nucleosome mobility without decompaction
🔹Cohesin prevents neighboring domain mixing

Full story & movies👇

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 28

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing.
www.biorxiv.org/cgi/content/...

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 29

Thank you so much!

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 29

Thank you for the nice list. Could you add me to the list?

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 28

🧩 Also highly relevant: a modeling study from Bin Zhang group predicts condensed euchromatin domains with active protrusions. Perfectly complementary to our findings! 3
www.biorxiv.org/content/10.1...

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 28

We thank all the coauthors: @katsuminami.bsky.social‬, Tamura-san, Ashwin-san, Higashi-san, Nishiyama-san, Kanemaki-san, Sasai-san @lschermelleh.bsky.social‬, Toyoda-san. 🙏Special thanks to @healthcare.nikon.com‬ for 3D-SIM support. Also see our related paper‪@TICB bit.ly/4fW4cfL 2

Is euchromatin really open in the cell?

Growing evidence has demonstrated that strings of nucleosomes, consisting of DNA wrapped around core histones [1], are rather irregularly and dynamically folded as chromatin in the cell (Figure 1A,B) ...

bit.ly

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 28

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing.
www.biorxiv.org/cgi/content/...

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Reposted by Kazuhiro Maeshima

Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Jul 23

📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...

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Reposted by Kazuhiro Maeshima

Tree-thinkers Lab✨分子生命史研究室 @kurakulabmsm.bsky.social · Jul 23

New chapter of sex determination, with sharks and rays!

Our study, utilizing embryonic samples from cooperative aquariums, revealed the ancient sex chromosome origin and unique sex determination potentials.

Published today in PNAS
www.pnas.org/doi/full/10....

#Squalomix

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Reposted by Kazuhiro Maeshima

semeigazin.bsky.social @semeigazin.bsky.social · Jul 16

My #PhD defense work is on #Biorxiv!
Link: biorxiv.org/content/10.110…
Using single-molecul#imagingng, we showed that liquid-like BRD4-NUT condensates locally constrai#chromatinin vi#bromodomain-dependentnt crosslinking.
I thank my supervisor, Prof. @kazu-maeshima.bsky.social, for his support!

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Michalis Averof @michalis-averof.bsky.social · Jul 13

We've just been awarded a grant to study the cellular basis of regeneration – to track the progenitors of sensory organs in the context of leg regeneration, in our favourite crustacean tinyurl.com/parhyale, based on live imaging and cell tracking. The project involves some cool collaborations... 1/3

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · Jul 10

Our new preprint 🧬🔗 www.biorxiv.org/content/10.1...
BRD4-NUT forms liquid-like condensates that locally constrain nucleosomes via BRD4-mediated crosslinking— physical control of #chromatin by #LLPS transcription condensates. @semeigazin.bsky.social @katsuminami.bsky.social @masaashimazoe.bsky.social

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Konrad Chudzik @konrad-chudzik.bsky.social · Apr 14

🚨 Preprint alert 🚨
Excited to share our work on "Ab-trapping," an antibody artifact causing misleading peripheral ("rim") staining in imaging & genomics (IF, CUT&Tag, CUT&RUN). Antibodies fail to penetrate structures, accumulating at the periphery. A 🧵👇
doi.org/10.1101/2025...

Ab-trapping - a peripheral staining artifact in antibody-based microscopy and genomics

Antibodies (Ab) are essential for detecting specific epitopes in microscopy and genomics, but can produce artifacts leading to erroneous interpretations. Here, we characterize a novel artifact, Ab-tra...

doi.org

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Reposted by Kazuhiro Maeshima

Akis (Argyris) Papantonis @akispapantonis.bsky.social · Jun 19

This is a piece that I and @karsten-rippe.bsky.social discussing a lot, and a topic that is very close to my heart. The editors @naturerevgenet.bsky.social gave us the stage to do so, and the final version of our review is now available under this link: rdcu.be/erP1u

A short thread follows 1/n

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Reposted by Kazuhiro Maeshima

Karsten Rippe @karsten-rippe.bsky.social · Jun 19

How do transcription compartments form — and does phase separation drive gene expression? I enjoyed discussing these questions with @akispapantonis.bsky.social a lot, and we put our thoughts together for @naturerevgenet.bsky.social, now out at rdcu.be/erP1u

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Thiago Carvalho @cyrilpedia.bsky.social · Jun 19

Tools
"Evidence suggests that acetyl-CoA metabolism is highly compartmentalized in mammalian cells. Yet methods to measure acetyl-CoA in living cells are lacking. Herein, we engineered an acetyl-CoA biosensor from the bacterial protein PanZ and circularly permuted green fluorescent protein (cpGFP)."

A genetically encoded fluorescent biosensor for visualization of acetyl-CoA in live cells

Smith et al. engineered a “turn on” fluorescent biosensor (dubbed “PancACe”) for acetyl-CoA from a fusion of the proteins PanZ and circularly permuted GFP. PancACe measures changes in acetyl-CoA level...

www.cell.com

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Reposted by Kazuhiro Maeshima

Lennart Hilbert @lennarthilbert.bsky.social · Jun 18

Very smart way to distinguish between eu/heterochromatin for single nucleosome tracking.

"Replication-dependent histone labeling dissects the physical properties of euchromatin/heterochromatin in living human cells"

From @kazu-maeshima.bsky.social lab

www.science.org/doi/10.1126/...

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Kazuhiro Maeshima @kazu-maeshima.bsky.social · Jun 18

Our new review paper is out@JMB!🧬
@katsuminami.bsky.social‬ @semeigazin.bsky.social‬ ‪@kakonakazato.bsky.social‬ discuss DNA accessibility in euchromatin and heterochromatin of live cells.
Free access: authors.elsevier.com/a/1lHgb54HFZ...
Also check our previous work: www.science.org/doi/10.1126/...

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Reposted by Kazuhiro Maeshima

Nature @nature.com · Jun 4

Losing the Y chromosome seems to make cancer cells more aggressive in men and the phenomenon may even spread between cells

https://go.nature.com/3Zh7bs7

Cancer more deadly when tumours lack Y chromosomes — and the loss could be contagious

Losing the Y chromosome seems to make cancer cells more aggressive in men and the phenomenon may even spread between cells.

go.nature.com

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