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Reposted by Hana Polasek-Sedlackova

altmeyerlab @altmeyerlab.bsky.social · 5d

A comprehensive genetic catalog of human double-strand break repair | Science www.science.org/doi/10.1126/...

A comprehensive genetic catalog of human double-strand break repair

The analysis of DNA sequence outcomes provides molecular insights into double-strand break (DSB) repair mechanisms. Using parallel in-pool profiling of Cas9-induced insertions and deletions (indels) w...

www.science.org

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Reposted by Hana Polasek-Sedlackova

dimitristypas.bsky.social @dimitristypas.bsky.social · Aug 12

@natsmb.nature.com has published three pieces on scientists returning to their country of origin after working abroad. www.nature.com/nsmb/article... Two more will come out. The most recent one by @hanasedlackova.bsky.social hits home for me, a very interesting read.
www.nature.com/articles/s41...

Returning home: there and back again in Czechia - Nature Structural & Molecular Biology

Returning to Central Europe was appealing and challenging. Here, I reflect on my part in rebuilding a research career in Czechia and fostering a collaborative scientific community across the region.

www.nature.com

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Reposted by Hana Polasek-Sedlackova

Nature Structural & Molecular Biology @natsmb.nature.com · Aug 8

ICYMI: New online: Returning home: there and back again in Czechia

Returning home: there and back again in Czechia

Nature Structural & Molecular Biology, Published online: 07 August 2025; doi:10.1038/s41594-025-01642-1Returning to Central Europe was appealing and challenging. Here, I reflect on my part in rebuilding a research career in Czechia and fostering a collaborative scientific community across the region.

go.nature.com

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Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Aug 7

Special thanks to belongs to editorial and production team of @natsmb.nature.com for their excellent work highlighting this important topic! ✨️

Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Aug 7

👉 There are many chapters and experiences behind this journey. This article touches only some of them. If you are considering establishing lab in Czechia feel free to reach out.

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Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Aug 7

This journey has been possible thanks to kind support of my mentors, dedicated colleagues, generous funding #GACR, #ERAfellowship, #EMBO and inspiring environment at our institute! 🙏

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Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Aug 7

📢 Excited to share my World View article, just published in @NatureSMB, where I reflect on returning to Czechia and building research group grounded in collaboration🤝, resilience and curiosity💡. www.nature.com/articles/s41...

https://nature.com/articles/s4159…

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Reposted by Hana Polasek-Sedlackova

Journal of Cell Biology @jcb.org · Aug 4

🧑‍🔬🎙️ When you publish with JCB, you can reach more of your community by submitting a short video! Want to promote your research in your own voice? 🎦 See our guidelines: rupress.org/jcb/pages/vi...
▶️ Watch video summaries from our authors: www.youtube.com/@JCellBiol

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Reposted by Hana Polasek-Sedlackova

Cahir O'Kane @cahirokane.bsky.social · Jul 23

Another reason to consider HaloTag as an alternative to GFP for tagging

Journal of Cell Biology @jcb.org · Jul 23

A Biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells. New tools developed by Anoop Kumar Yadav, Abhijeet Jadhav, Hana Polasek-Sedlackova @hanasedlackova.bsky.social and colleagues: rupress.org/jcb/article/...

#Technology #Chromatin #epigenetics #CellCycle

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Reposted by Hana Polasek-Sedlackova

Eva Petermann @petermannlab.bsky.social · Jul 23

We're very proud that our recent PhD graduate (and now postdoc) Rosie Wilkins' study on RNase H2 overexpression in human cells has been published in Oncogene.
www.nature.com/articles/s41...

Human RNase H2 upregulation counteracts oncogene- and chemotherapy-induced replication stress - Oncogene

Oncogene - Human RNase H2 upregulation counteracts oncogene- and chemotherapy-induced replication stress

www.nature.com

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Reposted by Hana Polasek-Sedlackova

Journal of Cell Biology @jcb.org · Jul 23

A Biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells. New tools developed by Anoop Kumar Yadav, Abhijeet Jadhav, Hana Polasek-Sedlackova @hanasedlackova.bsky.social and colleagues: rupress.org/jcb/article/...

#Technology #Chromatin #epigenetics #CellCycle

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Reposted by Hana Polasek-Sedlackova

Francesca Mattiroli @fmattiroli.bsky.social · Jul 22

Thank you, Melina, for the interview!

Nature Structural & Molecular Biology @natsmb.nature.com · Jul 21

New online: Q&A with Francesca Mattiroli

Q&A with Francesca Mattiroli

Nature Structural & Molecular Biology, Published online: 21 July 2025; doi:10.1038/s41594-025-01620-7Francesca Mattiroli leads a research group at the Hubrecht Institute in Utrecht in the Netherlands, delving into the dynamics of chromatin during DNA synthesis. Here we ask her about her research ethos, experience and interests.

go.nature.com

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Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Jul 23

Thank you, Pietro!

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Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Jul 23

🙏 We also thank all our founders #GACR, #ERAfellowship, #EMBO, and last but not least, the editors and production team of @jcb.org!

Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Jul 23

🙏 Congratulations to all authors for great effort and fantastic collaboration!

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Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Jul 23

👉 We provide proof-of-principle on how to capture and biochemically separate distinct protein variants involved in chromatin duplication & maintenance.

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Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Jul 23

👉 This was achieved by a combination of in silico design, chemical synthesis, and cell biology.

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Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Jul 23

👉 We showed that strategic adjustment of the linker length is an effective strategy in tailoring HaloTag ligands.

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Hana Polasek-Sedlackova @hanasedlackova.bsky.social · Jul 23

📢 For all HaloTag lovers💙💚💛❤️💜🩶 we are excited to share that our work, in which we developed a new biotin-HaloTag ligand, is now published in @jcb.org! Full link here: rupress.org/jcb/article-...

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Reposted by Hana Polasek-Sedlackova

Journal of Cell Biology @jcb.org · Jul 16

Yadav, Jadhav, @hanasedlackova.bsky.social et al present a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...

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Reposted by Hana Polasek-Sedlackova

Rockefeller University Press @rupress.org · Jul 16

In @jcb.org, the @hanasedlackova.bsky.social lab presents a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling & providing capabilities for efficient affinity capture of protein variants from live cells rupress.org/jcb/article/...

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Reposted by Hana Polasek-Sedlackova

Aga Gambus @agagambus.bsky.social · Jul 10

It is finally out 👍😀. Great job of the team (mostly absent from Bsky) and @m-saponaro.bsky.social

CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS url: academic.oup.com/nar/article/...

CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS

Abstract. Disassembly of the replication machinery (replisome) from chromatin is an active process driven by two ubiquitin ligases Cul2LRR1 and TRAIP, whic

academic.oup.com

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Reposted by Hana Polasek-Sedlackova

Francesca Mattiroli @fmattiroli.bsky.social · Jul 10

Apply to our PhD program!!

Hubrecht Institute @hubrechtinstitute.bsky.social · Jul 10

🧬 Looking for a PhD position in molecular or developmental biology? 🔬

✉️ The Hubrecht Institute now has its very own PhD program! Applications are open and will be accepted until September 15th. Read more here 👉 www.hubrecht.eu/hipp/

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Reposted by Hana Polasek-Sedlackova

Kai Johnsson @kjohnsson.bsky.social · Jul 3

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology

SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

www.nature.com

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Reposted by Hana Polasek-Sedlackova

Communications Biology @commsbio.nature.com · Jun 27

This review discusses the cellular pathways mediating Targeted Protein Degradation approaches and points to some gaps in our understanding of the biology of novel therapeutic tools such as PROTACs. doi.org/10.1038/s420...

Cellular parameters shaping pathways of targeted protein degradation - Communications Biology

This review discusses the cellular pathways mediating Targeted Protein Degradation approaches and points to some gaps in our understanding of the biology of novel therapeutic tools such as PROTACs.

doi.org

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