Lightnews — Scholar-powered news

Reposted by Shiori Iida

Sara Wickstrom @sarawickstrom.bsky.social · 9d

How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇

Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells - Nature Cell Biology

McCreery, Stubb et al. show that mechano-osmotic changes in the nucleus induce general transcriptional repression and prime chromatin for cell fate transitions by relieving repression of specific differentiation genes.

www.nature.com

10 74 170

Reposted by Shiori Iida

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 28

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing.
www.biorxiv.org/cgi/content/...

3 37 100

Reposted by Shiori Iida

Masa A. Shimazoe @masaashimazoe.bsky.social · Aug 28

Euchromatin is mostly condensed⁉️

With @shiori-iida.bsky.social , We revealed the detailed structure of euchromatin using super-resolution imaging.

Cohesin regulates the function of euchromatin via chromatin dynamics!
(1/n)

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 28

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing.
www.biorxiv.org/cgi/content/...

3 3 15

Shiori Iida @shiori-iida.bsky.social · Aug 30

Thank you, Amro! Grad to see you soon ☺️

1

Shiori Iida @shiori-iida.bsky.social · Aug 29

You can see that the slower subpopulation localizes like heterochromatin, while the faster one resembles euchromatin.

4

Shiori Iida @shiori-iida.bsky.social · Aug 29

One more movie!
Using the RL algorithm (from pnas.org/doi/10.1073/...),
@masaashimazoe.bsky.social analyzed our nucleosome tracking data and classified them by their mobility.
Hotter color = faster motion (Slow🔵→ 🟢 → 🟡 → 🟠 Fast)

2 16 45

Shiori Iida @shiori-iida.bsky.social · Aug 29

Thank you! ☺️

1

Shiori Iida @shiori-iida.bsky.social · Aug 28

I’m grateful to my supervisor
@kazu-maeshima.bsky.social
for leading this project, my amazing co–first author
@masaashimazoe.bsky.social
for putting this paper together, all coauthors, supporters, and lab members!

Check out our opinion article, too:

www.sciencedirect.com/science/arti...

www.sciencedirect.com

Shiori Iida @shiori-iida.bsky.social · Aug 28

Our new preprint is out!
We combined single-nucleosome imaging and 3D-SIM to reveal:
🔹 Euchromatin forms condensed domains, not open fibers
🔹Cohesin loss increases nucleosome mobility without decompaction
🔹Cohesin prevents neighboring domain mixing

Full story & movies👇

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Aug 28

Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social‬ @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing.
www.biorxiv.org/cgi/content/...

3 7 19

Reposted by Shiori Iida

Simone Reber @simonereber.bsky.social · Aug 15

FINALLY! Challenging to publish but we believe it is an important discovery: rdcu.be/eATFz

💚 Thanks to the team ‪@biswashere.bsky.social‬, Omar Muñoz, ✨Q✨ C. Hoege, B. Lorton, R. Nikolay ‪@matthewkraushar.bsky.social‬ @dshechter.bsky.social @gucklab.bsky.social @vasilyzaburdaev.bsky.social‬ 💚

Conserved nucleocytoplasmic density homeostasis drives cellular organization across eukaryotes

Nature Communications - Cells can regulate their mass density. Here, the authors demonstrate how eukaryotes establish and maintain a lower density in the nucleus than in the cytoplasm via pressure...

rdcu.be

1 25 55

Reposted by Shiori Iida

semeigazin.bsky.social @semeigazin.bsky.social · Jul 16

My #PhD defense work is on #Biorxiv!
Link: biorxiv.org/content/10.110…
Using single-molecul#imagingng, we showed that liquid-like BRD4-NUT condensates locally constrai#chromatinin vi#bromodomain-dependentnt crosslinking.
I thank my supervisor, Prof. @kazu-maeshima.bsky.social, for his support!

2 4

Reposted by Shiori Iida

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Jul 10

Our new preprint 🧬🔗 www.biorxiv.org/content/10.1...
BRD4-NUT forms liquid-like condensates that locally constrain nucleosomes via BRD4-mediated crosslinking— physical control of #chromatin by #LLPS transcription condensates. @semeigazin.bsky.social @katsuminami.bsky.social @masaashimazoe.bsky.social

1 26 51

Reposted by Shiori Iida

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Apr 30

📢 NIG Postdoc Fellowship Call!
www.nig.ac.jp/nig/career-d...
🧬We’re seeking motivated postdocs to join our lab. 🔬We study chromatin behavior in living cells using advanced live-cell imaging and quantitative analysis:
www.science.org/doi/10.1126/...
doi.org/10.2183/pjab...
Interested? Please apply!

11 13

Reposted by Shiori Iida

Sara Wickstrom @sarawickstrom.bsky.social · Apr 23

Check out this exciting preprint from @aznarbenitahlab.bsky.social discovering a new mode of crosstalk between YAP and the circadian regulator BMAL1. Congrats @juliabonjoch.bsky.social and @guiomarsolanas.bsky.social for fantastic work and thank you for the fun collaboration!

Júlia Bonjoch @juliabonjoch.bsky.social · Apr 23

Hi Bluesky community:)

Our new manuscript is now available as a preprint!⛅️
biorxiv.org/content/10.1...

1/ Here, we describe a novel BMAL1-YAP complex in epidermal cells, which is hijacked during ageing to control the expression of up-regulated inflammation genes through enhancer binding.🧵

BMAL1 and YAP cooperate to hijack enhancers and promote inflammation in the aged epidermis

Ageing is characterised by persistent low-grade inflammation that is linked to impaired tissue homeostasis and functionality. However, the molecular mechanisms driving age-associated inflammation rema...

biorxiv.org

2 3 9

Reposted by Shiori Iida

Rikki Garner @rikkigarner.bsky.social · Apr 24

In developing embryos, cells move a lot! Plenty of that movement is random. Is random cell mixing a feature or bug for tissue patterning? Turns out, it’s both! Excited to share the 1st preprint from my postdoc w/ Sean Megason @seanemcgeary.bsky.social and Allon Klein. 1/20 doi.org/10.1101/2025...

6 34 120

Reposted by Shiori Iida

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Apr 25

🎥 Video: See chromatin in action — each dot is a single nucleosome, dynamically fluctuating in a living HeLa cell (TMR-labeled, 50 ms/frame).
🔬 Original paper: www.science.org/doi/full/10....

Single-nucleosome imaging reveals steady-state motion of interphase chromatin in living human cells

Local chromatin motion remains steady during interphase, allowing cells to conduct housekeeping tasks under similar environments.

www.science.org

4 7

Reposted by Shiori Iida

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Apr 25

🧬My retrospective chromatin review is out @Proc Jpn Acad:
doi.org/10.2183/pjab...
The textbook view of #chromatin as 30-nm fibers is fading.
A new paradigm: chromatin forms #liquid-like domains—locally dynamic, yet globally stable at the chromosome level (viscoelastic)—supporting genome function. ✨

1 13 28

Reposted by Shiori Iida

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Mar 29

Our new paper is out@ScienceAdvances👇
www.science.org/doi/10.1126/...
🧬Our Repli-Histo labeling marks nucleosomes in euchromatin and heterochromatin in live human cells.
🔍 @katsuminami.bsky.social et al. have developed a chromatin behavior atlas within the nucleus. 1/2

1 25 70

Shiori Iida @shiori-iida.bsky.social · Mar 27

On top of that, I’m honored to receive the SOKENDAI Award from my university for my PhD work!

I can't thank @kazu-maeshima.bsky.social, my labmates, and all the NIG members enough for their support.

This recognition gives me even more motivation for the journey ahead!

8

Shiori Iida @shiori-iida.bsky.social · Mar 27

It took blood, sweat, tears, too much coffee☕️, and just the right amount of alcohol🍺—but I finally got my PhD!

Huge thanks to everyone supported me, especially my mentor @kazu-maeshima.bsky.social, labmates, friends, and family.

I’m excited to keep chasing big questions and growing as a scientist!

10

Reposted by Shiori Iida

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Mar 7

Our new preprint is out＠bioRxiv: www.biorxiv.org/content/10.1...
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in living cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/

3 40 120

Reposted by Shiori Iida

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Feb 20

Our new review on how the #chromatin domain is formed in the cell is now available @Curr Opin Struct Biol.📄✨
We critically discuss the domain formation mechanism from a physical perspective, including #phase-separation and #condensation.
📥 Free-download link:
authors.elsevier.com/a/1keGn,LqAr...

37 75

Reposted by Shiori Iida

@PhysGenCenter @physgencenter.bsky.social · Jan 24

Today in Science Advances: "Mature chromatin packing domains persist after RAD21 depletion in 3D". Researchers at CPGE integrated measurements of genome connectivity and physical structure to deepen our understanding of chromatin organization

#chromatin #genomics

www.science.org/doi/10.1126/...

Mature chromatin packing domains persist after RAD21 depletion in 3D

RAD21 contributes to nascent packing domain formation, but maturation requires nucleosome posttranslational modifications.

www.science.org

3 12

Reposted by Shiori Iida

Akane @hoyako.bsky.social · Dec 24

Thanks🎅🏼🦌
Merry Christmas all.
@shiori-iida.bsky.social @dai.tsujino
#NIGchrismtmass

2 6

Reposted by Shiori Iida

Kazuhiro Maeshima @kazu-maeshima.bsky.social · Nov 30

We are seeking a motivated postdoc to join our lab! Our research focuses on live-cell imaging/analysis to reveal how chromatin behaves in living cells and how its behavior contributes to cellular functions.
www.nature.com/articles/s41...
www.science.org/doi/10.1126/...
Interested? Please DM me!

2 40 66