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Reposted by Robin Journot 🔬| 🎾

Bardin Lab @bardinlab.bsky.social · 1d

The PhD work of @bboumard.bsky.social officially out in print! Happy to share this issue with our downstairs colleagues in the BDD @frelab.bsky.social @robinjournot.bsky.social

www.cell.com/developmenta...

Cell-type-specific nucleotide sharing through gap junctions impacts sensitivity to replication stress in Drosophila

Boumard et al. demonstrate gap-junction-dependent tissue-scale nucleotide sharing, which impacts cellular sensitivity to perturbation of nucleotide homeostasis and replication stress. Drosophila wing ...

www.cell.com

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 2d

Our paper is on the cover of @cp-devcell.bsky.social . Image: embryonic murine salivary-gland explants stained for fate determinants; p63 (cyan) and HES1 (yellow). Thanks to everyone involved.
doi.org/10.1016/j.de...

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Reposted by Robin Journot 🔬| 🎾

Bart Deplancke @bartdeplancke.bsky.social · 2d

🧵1/ Excited to share our new paper introducing a new #singlecell assay: scTF-seq, a high-throughput single-cell approach to explore how transcription factor (TF) dose shapes cell identity and reprogramming outcomes. 🔗 www.nature.com/articles/s41... Big congrats to the entire team @EPFL & @SIAT_China

Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq - Nature Genetics

This study introduces single-cell transcription factor (TF) sequencing, a single-cell barcoded and doxycycline-inducible TF overexpression approach that reveals dose-sensitive functional classes of TFs and cellular heterogeneity by mapping TF dose-dependent transcriptomic changes during the reprogramming of mouse embryonic multipotent stromal cells.

www.nature.com

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · 8d

"Contractile fibroblasts form a transient niche for the branching mammary epithelium."
Now out: rdcu.be/eIIKD
A great contribution from Jakub Sumbal, showing how stromal cells, and diverse fibroblast subsets, regulate branching morphogenesis.
@sumbalovakoledova.bsky.social @frelab.bsky.social

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Sep 8

From a FACS-contaminating cell population to a multi-organ project — learn the Behind the Story of our work on conserved signals in epithelial organogenesis 👉 www.sciencedirect.com/science/arti...

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 4

Yes! We think this mechanism goes way beyond glands. We're now exploring a broader range of tissues, stay tuned for what's coming next!

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 4

Great question! We haven’t inferred or directly measured mechanical forces yet, but in a follow-up project we’re actively investigating the mechanical component of the symmetry-breaking event. Very curious to see how it ties into YAP localization and happy to discuss more!

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Reposted by Robin Journot 🔬| 🎾

Bardin Lab @bardinlab.bsky.social · Jun 13

Students (Master's/PhD), do not miss this fantastic Developmental Biology course of @sorbonne-universite.fr and @institutcurie.bsky.social - It is FREE and open to international students, however, room and board + travel are not included.

training.institut-curie.org/courses/deve...

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

This work was funded by @institutcurie.bsky.social, ‪‪‪@cnrsbiologie.bsky.social, @psl-univ.bsky.social, @agencerecherche.bsky.social , @frm-officiel.bsky.social, @cerclefser.bsky.social, @fondationarc.bsky.social and @worldwidecancer.bsky.social.

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

Full publication in @cp-devcell.bsky.social : authors.elsevier.com/c/1lM285Sx5g...

authors.elsevier.com

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

Despite distinct germ layer origins and divergent adult functions, all 4 glands rely on the same YAP–Notch–p63 circuit to specify fate and pattern.
We propose an updated hourglass model where this conserved module acts as a developmental bottleneck in organogenesis.

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

In regeneration, we saw the same transitional state: YAP⁺p63⁺HES1⁺.
→ Developmental programs are reused for repair.

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

YAP acts then as a capacitor, counting the number of cell within the tissue before allowing cells to differentiate.

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

We found the key: YAP.
Initially nuclear in all cells, it becomes cytoplasmic in internal cells—coinciding with symmetry breaking and fate commitment. When we forced YAP activity, organoids didn’t break symmetry—cells stayed in a stem-like state, co-expressing p63 & HES1.
Same results in embryos!

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

But why do organoids stay homogeneous until ~13 cells?
Theoretical models say symmetry can break in 2 cells.
Here, an active mechanism seems to delay fate decisions early on.

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

We then asked: what regulates this decision?
Notch activation (in vivo or organoids) forced luminal fate.
Notch inhibition blocked it.
→ Notch is a gatekeeper of luminal identity.
We further demonstrated that symmetry breaking appeared through lateral inhibition.

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

We observed that fate segregation (K5 outside, K8 inside) happened not over time, but when organoids reached 13–21 cells.
→ This suggests size-dependent symmetry breaking drives fate commitment.

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

All 4 glands form 2-layered epithelia: K5⁺ basal cells on the outside, K8⁺ luminal facing the lumen.
But where does this structure come from during development? We sorted basal stem cells and grew organoids from all 4 tissues. They reactivated multipotency and gave rise to both cell types in vitro.

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Jul 1

🧵Just out !
We reveal how 4 branched epithelia—mammary, lacrimal, salivary & prostate—use a conserved YAP–Notch–p63 circuit to self-organize during development & regeneration.
Here’s the story👇
authors.elsevier.com/c/1lM285Sx5g...
Work done in @frelab.bsky.social at ‪
‪ @institutcurie.bsky.social

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Reposted by Robin Journot 🔬| 🎾

The EMBO Journal @embojournal.org · Apr 7

Notch1 activation in basal cells triggers a progressive basal-to-luminal cell-fate switch in the mouse mammary gland but also in adult cells of the lacrimal, salivary and prostatic glands
Silvia Fre and coworkers @frelab.bsky.social @institutcurie.bsky.social
www.embopress.org/doi/full/10....

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Reposted by Robin Journot 🔬| 🎾

Cornelia Schwayer @cschwayer.bsky.social · Jan 15

I am very excited to share our most recent work on how ‘Cell heterogeneity and fate bistability drive tissue patterning during intestinal regeneration’ ☺️ Thanks a lot to @silviabarbiero.bsky.social @davidbrueckner.bsky.social @priscaliberali.bsky.social
@ehannezo.bsky.social and all the co-authors!

Prisca Liberali @priscaliberali.bsky.social · Jan 15

A perfect start in the new year! 🌱 Regenerating tissues are just an amazing spatio temporal controlled process! Our new manuscript explores how cell heterogeneity and bistability orchestrate intestinal regeneration. 🧵 biorxiv.org/cgi/content/...

Cell heterogeneity and fate bistability drive tissue patterning during intestinal regeneration

Tissue regeneration relies on the ability of cells to undergo de novo patterning. While tissue patterning has been viewed as the transition from initially identical un-patterned cells to an arrangemen...

biorxiv.org

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Reposted by Robin Journot 🔬| 🎾

Hani Goodarzi @genophoria.bsky.social · Dec 5

@thejohnnyyu.bsky.social, @therealnima.bsky.social
, Kevan Shokat, and I are excited to announce a historic achievement by our team at @vevotherapeutics.bsky.social: tinyurl.com/4rrvap3t

Vevo Therapeutics to Release World's Largest Atlas of Single-Cell Transcriptomic Data, Tahoe-100M, to Map How Drugs Impact Patient Cells and Accelerate Discovery of New Drugs

/PRNewswire/ -- Vevo Therapeutics, a biotechnology company using its Mosaic technology and next generation AI to uncover better drugs for more patients,...

www.prnewswire.com

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Robin Journot 🔬| 🎾 @robinjournot.bsky.social · Nov 19

A new platform, a new post!
Our #Stemcell #DevBio and #Regeneration
story by Silvia Fre's lab at InstitutCurie is on biorXiv.
Link: tinyurl.com/2uspcd3u

We reveal how 4 epithelia self-organize during embryonic development and regeneration.

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