The hypothalamus is a brain region that plays a key role in coordinating fundamental biological functions1. However, our understanding of the underlying cellular components and neurocircuitries have, until recently, emerged primarily from rodent studies2,3. Here we combine single-nucleus sequencing of 433,369 human hypothalamic cells with spatial transcriptomics, generating a comprehensive spatio-cellular transcriptional map of the hypothalamus, the ‘HYPOMAP’. Although conservation of neuronal cell types between humans and mice, as based on transcriptomic identity, is generally high, there are notable exceptions. Specifically, there are significant disparities in the identity of pro-opiomelanocortin neurons and in the expression levels of G-protein-coupled receptors between the two species that carry direct implications for currently approved obesity treatments. Out of the 452 hypothalamic cell types, we find that 291 neuronal clusters are significantly enriched for expressio

The hypothalamus is a brain region that plays a key role in coordinating fundamental biological functions1. However, our understanding of the underlying cellular components and neurocircuitries have, until recently, emerged primarily from rodent studies2,3. Here we combine single-nucleus sequencing of 433,369 human hypothalamic cells with spatial transcriptomics, generating a comprehensive spatio-cellular transcriptional map of the hypothalamus, the ‘HYPOMAP’. Although conservation of neuronal cell types between humans and mice, as based on transcriptomic identity, is generally high, there are notable exceptions. Specifically, there are significant disparities in the identity of pro-opiomelanocortin neurons and in the expression levels of G-protein-coupled receptors between the two species that carry direct implications for currently approved obesity treatments. Out of the 452 hypothalamic cell types, we find that 291 neuronal clusters are significantly enriched for expressio

HYPOMAP ofrece valiosa información para comprender los circuitos que regulan el apetito, el equilibrio energético, el sueño y la respuestas al estrés ...

HYPOMAP integrates single-nucleus RNA sequencing and spatial transcriptomic data to create a comprehensive spatio-cellular map of the human hypothalamus.

HYPOMAP integrates single-nucleus RNA sequencing and spatial transcriptomic data to create a comprehensive spatio-cellular map of the human hypothalamus.

A high-resolution spatial map of the human hypothalamus makes it possible to identify specific cells, determine their exact location and analyze their neighboring cells.

The hypothalamus is a brain region that plays a key role in coordinating fundamental biological func

The hypothalamus is a brain region that plays a key role in coordinating fundamental biological func