#Ferroptosis of #MuscleStemCell in Myopathy #PeripheralArteryDisease

scRNAseq CD56+ MuSC
4 perfused/thigh vs 4 ischemic/calf muscle👤

Interesting Lipofuscin stain pattern
Ferroptosis also in non-satellite cells?🤔

Ulka Sachdev lab #ATVB 2023
www.ahajournals.org/doi/abs/10.1...

An important paper on Macrophage-#MuscleStemCell crosstalks in regenerative dynamics of ischemic #SkeletalMuscle #CriticalLimbThreateningIschemia

Proinflammatory niche perturbs MuSC proliferation-differentiation in #LimbIschemia

#GenomeMedicine 2023
genomemedicine.biomedcentral.com/articles/10....

#MuscleStemCell #SatelliteCell express low-level but Functional FLT1, NRP1, NRP2 proteins

VEGFA-Flt1 cascade protect MuSC from Apoptosis in cluture & ⏫#SkeletalMuscle regeneration (BaCl2, Cardiotoxin) & ameliorate muscle injury in Mdx mice

#eLife 2024
elifesciences.org/articles/73592

#PolyamineMetabolism #SkeletalMuscle #Aging

Depletion of S-adenosylmethionine (SAM, methyl donor) ➡️loss of #Heterochromatin➡️#MuscleStemCell #Aging

⏫SAM or⏬#SpermidineSynthase by MCHA▶️
⏫#SkeletalMuscle regeneration

#NatMetab 2024
www.nature.com/articles/s42...
pmc.ncbi.nlm.nih.gov/articles/PMC...

Landmark revisit

Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF & Notch Signaling

#MuscleStemCell VEGF-A recruits #EndothelialCell

EC DDL4⏫MuSC self-renewal

Mayank Verma Atsushi Asakura lab Cell Stem Cell 2018
www.cell.com/cell-stem-ce...

Muscle-Mural Lineage fates of embryonic TNAP+ progenitor (e11.5)

Skeletal myocyte
#MuscleStemCell 4%
#SmoothMuscleCell 7%
#Pericyte 20%

Fate window closed by e14.5

TNAP-CreERT2::nLacZ or Confetti🐭

Giulio Cossu lab #VasPharma 2025
www.sciencedirect.com/science/arti...