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florianwollweber.bsky.social
Florian Wollweber
@florianwollweber.bsky.social
580 followers 800 following 10 posts
Imaging Asgard archaea and other strange organisms @pilhoferlab.bsky.social and soon @embl.org Grenoble | 🦠❄️🔬#teamtomo embl.org/wollweber
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florianwollweber.bsky.social
Florian Wollweber @florianwollweber.bsky.social · Mar 21
Asgard archaea are full of surprises, this time we found (tiny) microtubules (snapshots of microtubule evolution?!) - Check out our new paper on the cytoskeleton of Lokiarchaeum ossiferum: www.cell.com/cell/fulltex...
pilhoferlab.bsky.social
Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · Mar 21
Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6
Credit: Margot Riggi (@margotriggi.bsky.social), Max-Planck Institute of Biochemistry (@mpibiochem.bsky.social)
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Reposted by Florian Wollweber
jpkreysing.bsky.social
Jan Philipp Kreysing @jpkreysing.bsky.social · 1d
❓What if you could peek between your tilt series ❓ We are proud to introduce cryoTIGER -- deep-learning based tilt interpolation for enhanced reconstruction in cryo-ET. A great collaboration between Turoňová lab, @becklab.bsky.social, @hummerlab.bsky.social #teamtomo www.nature.com/articles/s42...
cryoTIGER: deep-learning based tilt interpolation generator for enhanced reconstruction in cryo electron tomography - Communications Biology
Deep learning-based interpolation using cryoTIGER enhances angular sampling in cryoET, improving 3D reconstructions, particle localization, and structural recovery without increasing electron dose.
www.nature.com
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Reposted by Florian Wollweber
mike-wieczorek.bsky.social
Wieczorek Lab @mike-wieczorek.bsky.social · 1d
Huge congratulations to Wieczorek Lab PhD student Bin Cai and now-PI Jingwei Xu @xujwet.bsky.social on figuring out the surprisingly complex structure of the A-C linker crosslinking microtubule triplets in Tetrahymena basal body centrioles! www.science.org/doi/10.1126/...
Structure and assembly of the A-C linker connecting microtubule triplets in centrioles
Multiscale cryo-EM of centrioles reveals the structure and polymorphism of the A-C linker connecting microtubule triplets.
www.science.org
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Reposted by Florian Wollweber
denkenny.bsky.social
Kenneth Ehses @denkenny.bsky.social · 2d
✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv

Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo

🔗 www.biorxiv.org/content/10.1...
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Reposted by Florian Wollweber
pilhoferlab.bsky.social
Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · 2d
🧐Checkout our preprint revealing the stepwise firing💥 mechanism of a Contractile Injection System @xujwet.bsky.social[email protected] trapped the complex by structure-guided engineering🧪 in multiple intermediate states and imaged them by multimodal #cryoEM❄️🔬
www.biorxiv.org/content/10.1...
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Reposted by Florian Wollweber
pilhoferlab.bsky.social
Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · 2d
Congrats🥳 to @mike-wieczorek.bsky.social, Bin Cai, and our former lab member Jingwei Xu @xujwet.bsky.social to their multiscale imaging 🔬 story of the centriolar AC linker #teamtomo
www.science.org/doi/10.1126/...
Structure and assembly of the A-C linker connecting microtubule triplets in centrioles
Multiscale cryo-EM of centrioles reveals the structure and polymorphism of the A-C linker connecting microtubule triplets.
www.science.org
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Reposted by Florian Wollweber
daumlab.bsky.social
The Daum Lab @daumlab.bsky.social · 5d
Out in Science Advances: Our #cryoEM structure of HFTV1, a virus infecting the halophile #archaea. *First full atomic structure (containing all structural proteins) of any tailed virus!* Congrats and thanks to all co-authors and our fantastic collaborators! www.science.org/doi/10.1126/...
Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail
This structure of an archaeal tailed virus (arTV) provides detailed insights into arTV assembly and infection mechanisms.
www.science.org
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Reposted by Florian Wollweber
archaellum.bsky.social
Sonja-Verena Albers @archaellum.bsky.social · 9d
Look at this nice cover: beautiful archaella, image made by @sshamphavi.bsky.social !

Thanks also to Marta Rodeiguez for keeping the EM im shape in @ottlab.bsky.social lab!

At @biologyunifreiburg.bsky.social at @uni-freiburg.de @sfb1381.bsky.social @cibss.bsky.social
natmicrobiol.nature.com
Nature Microbiology @natmicrobiol.nature.com · 9d
Some keen Observations in Our October issue!

🦠 Cancer microbiome
🌊 Marine microbes in warming oceans
🌐 broad-range phages
🧬 genetically minimised Salmonella
🔧 Viral infection mechanisms
🧪 Oxaloacetate antiviral defence

and more, here: www.nature.com/nmicrobiol/v...
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Reposted by Florian Wollweber
fonamental.bsky.social
Javier del Campo @fonamental.bsky.social · 9d
A New Age of Advanced Volume Microscopy for Protists onlinelibrary.wiley.com/doi/10.1111/... #protists #protistsonsky
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Reposted by Florian Wollweber
floravincent.bsky.social
Flora Vincent @floravincent.bsky.social · 12d
Dear all, registration for the upcoming EMBO/EMBL Symposium on "the cellular mechanics of symbiosis" is now OPEN 🤩 ! Feel free to register ! www.embl.org/about/info/c...
#EESSymbiosis #ProtistsOnSky #SymbioSky
@hassansalem.bsky.social @berasymbionts.bsky.social @embl.org
The cellular mechanics of symbiosis: sensing friend from foe
www.embl.org
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Reposted by Florian Wollweber
nexperion.net
Guenter Resch @nexperion.net · 18d
Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026
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Reposted by Florian Wollweber
cellarchlab.com
Ben Engel @cellarchlab.com · 15d
Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on!

#TeamTomo #PlantScience 🧪 🧶🧬 🌾
elifesciences.org/articles/105...
1/🧵
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Reposted by Florian Wollweber
cellarchlab.com
Ben Engel @cellarchlab.com · 20d
A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾
elife.bsky.social
eLife @elife.bsky.social · 21d
🌱 Using ‘compelling’ methods, including #CryoET, researchers mapped spinach thylakoid membranes at single-molecule precision, revealing how photosynthetic complexes are organised and settling long-standing debates on chloroplast architecture.
buff.ly/j3TSIkn
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Reposted by Florian Wollweber
maxplanck.de
Max-Planck-Gesellschaft @maxplanck.de · 22d
Truly chuffed for our fearless food physicists @mpipks.bsky.social + collabs from AT @istaresearch.bsky.social, IT & ES who won this year’s Ig Nobel - the #NobelPrize of hearts❤️for cracking the science of perfect pasta !🍝Kudos to all for intrepidly consuming lots of cheese in the name of science!😋
The Secret to a Smooth Pasta Sauce Wins Ig Nobel Prize
Italian researchers studied how the ingredients of the traditional Roman dish cacio e pepe emulsify into a creamy sauce, winning the 2025 Physics Ig Nobel Prize.
www.the-scientist.com
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Reposted by Florian Wollweber
archaellum.bsky.social
Sonja-Verena Albers @archaellum.bsky.social · 23d
Happy to share that our story on the bacterial archaellum was published today in @natmicrobiol.nature.com
www.nature.com/articles/s41...

Congrats to the authors: @sshamphavi.bsky.social @loumollat.bsky.social @mariejoest.bsky.social Najwa Taib and @sgribaldo.bsky.social
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Reposted by Florian Wollweber
pilhoferlab.bsky.social
Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · Sep 10
Story on our Asgard microtubule paper by Nikki Greenwood: www.quantamagazine.org/tiny-tubes-r...
Thanks to all interviewees🎤
Tiny Tubes Reveal Clues to the Evolution of Complex Life | Quanta Magazine
Scientists have identified tubulin structures in primitive Asgard archea that may have been the precursor of our own cellular skeletons.
www.quantamagazine.org
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Reposted by Florian Wollweber
tbharat-lab.bsky.social
Tanmay Bharat @tbharat-lab.bsky.social · Aug 31
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social

www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
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Reposted by Florian Wollweber
centriolelab.bsky.social
Paul & Virginie - Centriole Lab @centriolelab.bsky.social · Aug 28
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
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Reposted by Florian Wollweber
svenklumpe.bsky.social
Sven Klumpe @svenklumpe.bsky.social · Aug 23
If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...
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Reposted by Florian Wollweber
nadlerlab.bsky.social
André Nadler @nadlerlab.bsky.social · Aug 21
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...
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Reposted by Florian Wollweber
archaellum.bsky.social
Sonja-Verena Albers @archaellum.bsky.social · Aug 20
🚨 Imagine a bacterium that refuses to follow the textbook:
It grows as tangled filaments, divides unevenly, reshapes its own membranes… and even builds grappling hooks.
Meet Litorilinea aerophila — and here’s why it blew our minds.
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Reposted by Florian Wollweber
archaellum.bsky.social
Sonja-Verena Albers @archaellum.bsky.social · Aug 20
Who would have thought I would ever publish an article about a bacterium?
But here it is, but of course only because Litorilinea aerophilum actually has an archaellum!

Here is what we learned about its cell structure and other surface appendages:
www.biorxiv.org/content/10.1...
Filamentous growth, cell envelope architecture, and surface appendages of a member of the Chloroflexota, Litorilinea aerophila
Litorilinea aerophila, a filamentous bacterium of the phylum Chloroflexota (class Caldilineae), exhibits unique morphological and cell envelope features that challenge traditional bacterial models. In...
www.biorxiv.org
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Reposted by Florian Wollweber
jennysachweh.bsky.social
Jenny Sachweh @jennysachweh.bsky.social · Aug 18
My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!

Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!

Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo
The small GTPase Ran defines nuclear pore complex asymmetry
Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…
www.sciencedirect.com
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Reposted by Florian Wollweber
svenklumpe.bsky.social
Sven Klumpe @svenklumpe.bsky.social · Aug 15
Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...
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Reposted by Florian Wollweber
sjorsscheres.bsky.social
Sjors Scheres @sjorsscheres.bsky.social · Aug 12
Sofia Lövestam's vault structure is now online in Structure @cellpress.bsky.social. 🥳

How beautifully explorative is #cryoEM! We never planned to do this. The vaults just came down with #amyloids from human brain tissue, and Sofia decided to have a closer look.

www.cell.com/structure/fu...
Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps
Lövestam and Scheres present a cryo-EM structure of vault particles, found during tau filament extraction from progressive supranuclear palsy (PSP) brain tissue. They reveal a symmetry shift at the va...
www.cell.com
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Reposted by Florian Wollweber
pilhoferlab.bsky.social
Pilhofer Lab 🦠❄️🔬 @pilhoferlab.bsky.social · Aug 12
Check out 🧐 our new preprint 📜:
www.biorxiv.org/content/10.1...
Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠
#microsky #teamtomo #Chloroflexota
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