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Reposted by Arindam Ghosh

PicoQuant @picoquant.bsky.social · 13d

👏 Congratulation to the 🏆 winners of Student Award held by #PicoQuant during 30th anniversary of our annual workshop on Single Molecule Spectroscopy and Ultra Sensitive Analysis in the Life Sciences. #WS30
We were proud to once again support the next generation of scientists. #PicoQuantStudentAward

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Sauer Lab @sauer-lab.bsky.social · Aug 19

🚨
New Preprint!
Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons.

▶️ www.biorxiv.org/content/10.1...

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Kai Johnsson @kjohnsson.bsky.social · Jul 3

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology

SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

www.nature.com

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Andrey Klymchenko @aklymchenko.bsky.social · Jun 17

Great news: I received ERC Advanced grant #ERCAdG @erc.europa.eu CaptuRel! It aims at intelligent nanomaterials undergoing bioinspired cycle of capture and photorelease of bioactive molecules for sensing and controlling (bio)chemical gradients. Thanks to my Team, @cnrs.fr and @unistra.fr!

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Joachim Goedhart @joachimgoedhart.bsky.social · Jun 26

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...

$Fig. 4 Super-resolution microscopy in live and fixed cells with Rhobin. (A) Rhobin transiently binds rhodamine molecules and thereby constantly replenishes signal lost through photobleaching of dyes. (B-D) No-wash STED microscopy of live U2OS cells transiently expressing LifeAct-Rhobin2 labeled with 100 nM JFX650. (C, D) Zoom-ins of regions highlighted in (B) imaged either with confocal or STED microscopy. Scale bar: 20 μm (B) and 10 μm (C, D). (E-H) Rhobin enables timelapse STED microscopy of fast ER dynamics with minimal signal loss. U2OS stably expressing N-terminal tag fusions of SEC61B were stained with 1 μM Halo-JFX650 (HaloTag7, reHaloF) or 2 μM SiRhP (Rhobin2) and imaged at a frame rate of 0.387 fps for at least 100 time points. (F, G) Selected frames from timelapse acquisitions of Rhobin2:SEC61B labeled with SiRhP (F) or HaloTag7:SEC61B labeled with Halo-JFX650 (G). Scale bar: 2 μm. (H) Signal .CC-BY-NC 4.0 International licenseavailable under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprintthis version posted June 25, 2025.;https://doi.org/10.1101/2025.06.24.661379doi:bioRxiv preprint 22 loss during timelapse imaging. Mean ± SD signal inside cells over time for indicated constructs. (I-K) PAINT-type super-resolution microscopy with Rhobin. Rhobin2-SEC61B was transiently expressed in U2OS cells and labeled with 5 nM SiRhP after chemical fixation. Imaging under no- wash conditions and near-TIRF illumination. (I) Repeated, but transient binding of SiRhP molecules to Rhobin2 at low nanomolar concentrations can be observed as intensity bursts in intensity time traces extracted from a diffraction-limited area. See movie S3 for raw data of binding-induced blinking. (J) Sum image across 10,000 frames of an image stack, simulating a diffraction-limited image. (K). Reconstructed image from localized molecules in the full stack.$

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Paul & Virginie - Centriole Lab @centriolelab.bsky.social · Jun 19

🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...

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Erdinc Sezgin @sciezgin.bsky.social · May 13

New paper out! Would you like to fluorescently label the plasma membrane in live, fixed or permeabilized cells? I tested different lipid structures and labelling approaches to decide what makes a good membrane labelling probe for such imaging experiments!
www.biorxiv.org/content/10.1...

Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure

Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membr...

www.biorxiv.org

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Luciano A. Masullo @lumasullo.bsky.social · May 7

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩

We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

JungmannLab @jungmannlab.bsky.social · May 7

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution

We are excited to present our latest work published in @natcomms.nature.com

www.nature.com/articles/s41...

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications

Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...

www.nature.com

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Anna-Karin Gustavsson @gustavssonlab.bsky.social · Apr 22

Collaborative work with the Shechtman Lab and @heilemannlab.bsky.social.

Check it out here: www.biorxiv.org/content/10.1...

One-click image reconstruction in single-molecule localization microscopy via deep learning

Deep neural networks have led to significant advancements in microscopy image generation and analysis. In single-molecule localization based super-resolution microscopy, neural networks are capable of...

www.biorxiv.org

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Arindam Ghosh @arindam92.bsky.social · Apr 22

www.researchsquare.com/article/rs-6...

Open-source Sub-Nanometer Stabilization System for Super-resolution Fluorescence Microscopy

Recent advances in fluorescence nanoscopy have pushed resolution to the 1-10 nm range, enabling the direct visualization of individual molecules even in crowded biological environments. Achieving this...

www.researchsquare.com

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Arindam Ghosh @arindam92.bsky.social · Apr 1

Thanks so much!

Arindam Ghosh @arindam92.bsky.social · Mar 25

Our work on lattice light-sheet TDI-DNA-PAINT and anti-CD20 immunotherapy antibodies is now featured in The Scientist magazine www.the-scientist.com. Many thanks to Sneha Khedkar for writing this fantastic piece. www.the-scientist.com/new-microsco...

New Microscopy Technique Challenges Therapeutic Antibody Classification

A super-resolution microscopy technique offers an unparalleled glimpse into how monoclonal antibodies bind to their targets on cancer cells to induce cell death.

www.the-scientist.com

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Sandrine Lévêque Fort 🔬 @slfort.bsky.social · Mar 4

🔬Happy to share our new preprint, where brightness is used to identify single molecule emission in 2D/3D, with a single camera ! Part of the PhD work of @laurent-le.bsky.social and Surabhi, great collaboration with @emmanuelfort.bsky.social from @instlangevin.bsky.social @ismolab.bsky.social #SMLM

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Angel A. Martí @angel-marti.bsky.social · Mar 4

Congratulations to Rice Chemistry Prof. Anna-Karin Gustavsson for receiving the NSF CAREER.
@gustavssonlab.bsky.social #NSFCAREER #RiceChemistry

Anna-Karin Gustavsson on LinkedIn: Rice’s Gustavsson receives NSF CAREER Award to investigate dynamics of…

Very honored and grateful to receive the NSF CAREER Award! Big thanks to the National Science Foundation (NSF) and to my fantastic research team and…

www.linkedin.com

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Jörg Enderlein @joergenderlein.bsky.social · Mar 11

Happy to share our newest paper in Ang. Chem. and big thanks to @nkaredla.bsky.social, @faldalf.bsky.social and @tao-chen.bsky.social 🙏 onlinelibrary.wiley.com/doi/10.1002/...

Leaflet‐specific Structure and Dynamics of Solid and Polymer Supported Lipid Bilayers

Polymer-supported or tethered lipid bilayers serve as versatile platforms for mimicking plasma membrane structure and dynamics, yet the impact of polymer supports on lipid bilayers remains largely un...

onlinelibrary.wiley.com

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Dr. Romain Fernandez @romainbioimage.bsky.social · Mar 7

🧪Paris Jussieu right now #standupforscience

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Institut Pasteur | 130 years of biomedical research @pasteur.fr · Mar 6

Science, research & public health face unprecedented attacks in the U.S.

We stand with our American colleagues & support the Stand Up for Science call.

Join the global march tomorrow, incl. in Paris! 🧪✊

📍 March 7, 13:30 – Place Jussieu
🔗 More info: standupforscience.fr

#StandUpForScience

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Jörg Enderlein @joergenderlein.bsky.social · Feb 18

I'm thrilled to share that our review article, "Molecular Level Super-Resolution Fluorescence Imaging," has now been published online (www.annualreviews.org/content/jour...)! We provide a comprehensive overview of fluorescence super-res methods that push the limits towards molecular-scale imaging.

Molecular Level Super-Resolution Fluorescence Imaging | Annual Reviews

Over the last 30 years, fluorescence microscopy, renowned for its sensitivity and specificity, has undergone a revolution in resolving ever-smaller details. This advancement began with stimulated emis...

www.annualreviews.org

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Biophysical Reports @biophysreports.bsky.social · Feb 14

Online now! Fluorescent labeling strategies for molecular bioimaging. Marcel Streit, Made Budiarta, Marvin Jungblut, and Gerti Beliu. www.cell.com/biophysrepor...

Fluorescent labeling strategies for molecular bioimaging

Super-resolution microscopy (SRM) has transformed biological imaging by circumventing the diffraction limit of light and enabling the visualization of cellular structures and processes at the molecula...

www.cell.com

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Sauer Lab @sauer-lab.bsky.social · Feb 12

🆕 Our new functionalized docetaxel probe enables superior visualization of dense mitotic spindle structures during cell division by expansion microscopy!

▶️ pubs.acs.org/doi/10.1021/...

Functionalized Docetaxel Probes for Refined Visualization of Mitotic Spindles by Expansion Microscopy

Visualizing the ultrastructure of mitotic spindles, the macromolecular machines that segregate chromosomes during mitosis, by fluorescence imaging remains challenging. Here we introduce an azido- and ...

pubs.acs.org

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Carolyn Bertozzi @carolynbertozzi.bskyverified.social · Feb 6

Check it out, powerful new method for optical imaging of the live ECM, from @hhmijanelia.bsky.social Group Leader Kayvon Pedram #proudofalumni #glycotime

www.nature.com/articles/s41...

Live imaging of the extracellular matrix with a glycan-binding fluorophore - Nature Methods

Rhobo6 is a cell-impermeable small-molecule fluorophore that displays reversible fluorogenic binding to glycans, making it a general, wash-free, and non-perturbative label for the extracellular matrix...

www.nature.com

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UNSW Single Molecule Science @singmolsci.bsky.social · Feb 7

Aaaand that’s a wrap at Asia Pacific Microscopy Congress 2025 in sunny Brisbane, attended by a big team from Single Molecule Science. Lots of exciting new 🔬 tools and translation in biomedical investigations. @ijayas.bsky.social @arindam92.bsky.social

Group photo of eight colleagues representing the dept, standing in front of the conference mural

Some colleagues sitting around a dinner table

A conference speaker standing at a lectern podium

Arindam Ghosh being introduced to the symposium by Liz Hinde.

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PicoQuant @picoquant.bsky.social · Feb 4

Want to explore the world of time-resolved fluorescence #microscopy? Sign up for our online course and benefit from theoretical as well as practical sessions in an interactive format. Topics: data analysis, FCS, FRET, FLIM, and more. May 13-16, 2025. ➡️ www.microscopy-course.org

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MichelleDigman @digmanlab.bsky.social · Feb 2

Thrilled and honored to present a plenary talk at the 13th Asia Pacific Microscopy Congress (APMC13) in Brisbane, Australia, on February 7, 2025, hosted by the Australian Microscopy and Microanalysis Society (AMMS).

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Erdinc Sezgin @sciezgin.bsky.social · Jan 30

New paper out💥 in Nano Letters @pubs.acs.org
High throughput single particle profiler with simple confocal imaging. We used this new method to study protein-lipid interactions, drug membrane interactions and biophysics of small particles from human donors: pubs.acs.org/doi/10.1021/...

Imaging Single Particle Profiler to Study Nanoscale Bioparticles Using Conventional Confocal Microscopy

Single particle profiling (SPP) is a unique methodology to study nanoscale bioparticles such as liposomes, lipid nanoparticles, extracellular vesicles, and lipoproteins in a single particle and high t...

pubs.acs.org

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