James Lloyd 🧬
@jamespblloyd.bsky.social
2.1K followers 1.8K following 290 posts
Synthetic AstroBotatnist. Engineer of synthetic gene circuits in plants. 🇬🇧🇪🇺 now in 🇦🇺. He/They. Google Scholar: http://shorturl.at/dnHVZ Compbio blog: badgrammargoodsyntax.com
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jamespblloyd.bsky.social
I am super excited to announce the first (brief) pre-print purely from my group:

Start right to end right: authentic open reading frame selection matters

www.biorxiv.org/content/10.1...

This marks a return to NMD research for me. NMD detects early stop codons but we need to find the true stop!
Boromir Meme: One does not simply select the longest ORF
Reposted by James Lloyd 🧬
slcuplants.bsky.social
Salicylic acid biosensor, SalicS1, tracks the plant immune hormone salicylic acid in real time - revealing propagation of hormone surge during plant pathogen advance

Latest biosensor from @xanderjones.bsky.social team
In Science doi.org/10.1126/scie...
Summary www.slcu.cam.ac.uk/news/new-bio...
Reposted by James Lloyd 🧬
ekaiserli.bsky.social
Marie Skłodowska-Curie PhD position in our lab @uofglasgow.bsky.social on Enhancing Climate-Resilient Crops by Innovative Priming Strategies. A network led by @goetheuni.bsky.social
cordis.europa.eu/project/id/101227217
International applicants are eligible. Application form:
pollenbiology.cz/page-2
Reposted by James Lloyd 🧬
alexdemendoza.bsky.social
Thrilled to share that the lab has been awarded a @wellcometrust.bsky.social Discovery Award to keep exploring the evolution of 6-methyladenine #6mA in Eukaryotes. We'll open postdoc (3️⃣) and tech (1️⃣) positions to start in 2026, please share with candidates or reach out if you’d like to join us.
Reposted by James Lloyd 🧬
plantevolution.bsky.social
Game changer for cell-based plant genetics: the labs of Caixia Gao & Jin-Long Qiu have developed very efficient self-replicating vectors and they just published a very nice proof-of-concept paper.
#plantscience
www.science.org/doi/10.1126/...
Reposted by James Lloyd 🧬
plantteaching.bsky.social
#NBIAST2025
Designer flowers for art and education
@nickdesnoyer.bsky.social
Combining art, design, and science.
Nick Desnoyer presenting his talk on art and flowers
jamespblloyd.bsky.social
Is it just me, or is it a little odd that OpenAI is encouraging deep fakes of people on social media (see example).

youtube.com/shorts/gTcdv...
🤖 Sora AI slop
YouTube video by 3reate Podcast
youtube.com
Reposted by James Lloyd 🧬
reskilab.bsky.social
Our review "Acylamino acid-releasing enzyme, a bifunctional protease with a potential role in aging" is now out in JXB with full citation. Please check it out.
AARE is deeply conserved in evolution. So it's far more than #plantscience.
academic.oup.com/jxb/article/...
Acylamino acid-releasing enzyme, a bifunctional protease with a potential role in aging
We consolidate information on Acylamino acid-releasing enzyme, a serine protease affecting plant development and aging, and emphasize its conserved feature
academic.oup.com
jamespblloyd.bsky.social
Why were they hired in the first place?! Don’t we have civil servants for a reason?
Reposted by James Lloyd 🧬
drmikebooth.bsky.social
It's Chem Nobel Prize season, so your reminder that DNA synthesis/Marvin Caruthers needs to win! chemsky 🧪

Without DNA synthesis there is NO *PCR*, *sequencing*, *cloning*, *CRISPR*, *siRNA*, *synthetic biology*, *mRNA vaccines*, *nucleic acid therapeutics*, *Nanotech*

What more am I missing?
Picard saying awesome job! give that man a medal
Reposted by James Lloyd 🧬
chloegirard.bsky.social
🚨 First pre-print from my team !!

TL;DR: presence of polymorphism (sequence differences between the homologous chromosomes) can *increase* the local rate of recombination in Arabidopsis thaliana, turning cold regions of the genome hot (purple v. grey) !
The recombination frequency (cM/Mb) along chromosome 4 of A. thaliana (female meiosis). 

In full hybrids (grey), where polymorphism is distributed all along chromosomes, most crossovers occur in the regions surrounding the Centromere (Cen), and very few at chromosome ends (Tel: telomeres). 

In lines where polymorphism is restricted to chromosome ends (purple), the local recombination rate increases drastically, at the expense of the non-polymorphic regions (yellow).
Reposted by James Lloyd 🧬
chenxinli2.bsky.social
Our new short article is online at Plant Biotech J (onlinelibrary.wiley.com/doi/10.1111/...)!

"Single Cell Multi-Omics Reveals Rare Biosynthetic Cell Types in the Medicinal Tree Camptotheca acuminata"

Product of an NSF-EAGER award that I was co-PI from 2023-2025 (www.nsf.gov/awardsearch/...).
Figure of the paper.
Reposted by James Lloyd 🧬
drruth.bsky.social
The HPV vaccine is doing what had been promised.
17 years after it became available, HPV infections decreased significantly in vaccinated people and unvaccinated people because of herd immunity. buff.ly/WvjQ1BS

h/t @boghuma.bsky.social

#medsky #pedsky 🛟🧪
Light blue background. Young woman with dark hair makes a sign of strength with her arm and she has a vaccine bandaid on her upper arm. It says "The HPV Vaccine Works!"
Reposted by James Lloyd 🧬
geminiteamlab.bsky.social
How do geminiviruses maximize their limited coding capacity? Our recent preprint uncovers splicing of viral transcripts as one more strategy used by this viral family. We show that RNA splicing is prevalent in the geminivirus TYLCV — and required for infectivity! www.biorxiv.org/content/10.1...
Pervasive splicing in a plant DNA virus
Viruses maximize their limited coding space through strategies that increase transcript and protein diversity. In mammalian viruses, splicing is a well-established mechanism for proteome expansion, ye...
www.biorxiv.org
Reposted by James Lloyd 🧬
greenrat.bsky.social
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.
jamespblloyd.bsky.social
Any chance that I can be added?
Reposted by James Lloyd 🧬
jxbotany.bsky.social
🌱📖 RESEARCH 🌱📖

Bascom et al. used CRIPSR/Cas9 to produce a septuple AUXIN RESPONSE FACTOR mutant in the model bryophyte Physcomitrium patens , revealing the developmental consequences of the complete loss of auxin-mediated gene activation 🌱📖

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪
Fig. 4.Model of auxin de-repression. When the auxin concentration in the nucleus is low (A), the effect of ARFas, as well as other non-ARF transcription factors, is limited by Aux/IAA-mediated recruitment of the TPL co-repressor, closing the locus. As auxin levels rise (B), AFBs ubiquitinate Aux/IAAs, thereby allowing all transcription factors to act on the locus. As a result, there is a significant difference in gene expression between baseline and auxin induction. In afb1,2,3,4 mutants (C), Aux/IAAs are not degraded, and therefore the promoter remains closed. In the iaa2mDII degron mutant (D), one of three Aux/IAAs is stabilized, thereby resulting in only partial promoter closure. In arfasept lines, Aux/IAAs are not recruited to the promoter. While gene expression remains diminished without activating ARFs, other non-ARF transcription factors are allowed to provide baseline gene expression. However, there is no auxin-mediated induction.