Stephane Vassilopoulos
@stefvass.bsky.social
Cell biologist @INSERM @Sorbonne_Univ_, clathrin, dynamin, neuromuscular diseases, electron microscopy, metal replicas #CLEM
🔬🇪🇺 🇬🇷 🇨🇵
🔬🇪🇺 🇬🇷 🇨🇵
Pinned
One of my favorite images: a platinum replica electron microscopy shot of an unroofed cancer cell migrating along collagen fibers (blue). It beautifully captures the clathrin-coated pits (red, ~100 nm) and the intricate network of branched actin filaments (orange) 🔬
Reposted by Stephane Vassilopoulos
My personal obituary for Richard Hynes now live on the Manchester Cell-Matrix Centre website. www.manchester-matrix.org/news/profess...
Professor Richard O. Hynes PhD FRS (1944-2026) - Manchester Centre for Cell-Matrix Research
www.manchester-matrix.org
January 30, 2026 at 4:45 PM
My personal obituary for Richard Hynes now live on the Manchester Cell-Matrix Centre website. www.manchester-matrix.org/news/profess...
Reposted by Stephane Vassilopoulos
This is finally out in J Neuroscience! A micropatterned presynapse-on-glass approach to resolve the nanoscale architecture and function of presynapses
www.jneurosci.org/content/earl...
www.jneurosci.org/content/earl...
January 30, 2026 at 9:49 AM
This is finally out in J Neuroscience! A micropatterned presynapse-on-glass approach to resolve the nanoscale architecture and function of presynapses
www.jneurosci.org/content/earl...
www.jneurosci.org/content/earl...
Reposted by Stephane Vassilopoulos
Excited to share our work engineering caveolin-derived nanoparticles “caveospheres”. We show their use for targeted transfection in cultured cells and effective targeted delivery and tumor killing in vivo.
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
A Modular Encapsulation System for Precision Delivery of Proteins, Nucleic Acids, and Small Molecules
Targeted nanoparticles have the potential to revolutionize therapeutics for medical applications. Here, we demonstrate the utility of a flexible precision nanovesicle delivery system for functional de...
pubs.acs.org
January 22, 2026 at 1:13 AM
Excited to share our work engineering caveolin-derived nanoparticles “caveospheres”. We show their use for targeted transfection in cultured cells and effective targeted delivery and tumor killing in vivo.
pubs.acs.org/doi/full/10....
pubs.acs.org/doi/full/10....
Reposted by Stephane Vassilopoulos
🚨The Neurocyto lab is branching out in our latest preprint! We used tubulin microinjection to directly visualize microtubule turnover in developing hippocampal neurons, demonstrating the presence of in-lattice repair and a selective stabilization in the nascent axon. Check below, or read on 🧵 1/9
Direct labeling of microtubule turnover reveals in-lattice repair and stabilization patterns in developing neurons
The microtubule cytoskeleton is the backbone of neuronal morphogenesis, driving the development of the dendrites and axon, and supporting trafficking to distant compartments. How neuronal microtubules...
www.biorxiv.org
January 12, 2026 at 7:14 PM
🚨The Neurocyto lab is branching out in our latest preprint! We used tubulin microinjection to directly visualize microtubule turnover in developing hippocampal neurons, demonstrating the presence of in-lattice repair and a selective stabilization in the nascent axon. Check below, or read on 🧵 1/9
Reposted by Stephane Vassilopoulos
Latest paper is now out and features on the cover of Science Advances 👉 tinyurl.com/vea5862x
Very grateful Igor Bonacossa Pereira, Dat Le & Massimo Hilliard @hilliardlab.bsky.social for their creativity & passion.
Read more about it 👉
tinyurl.com/56awjda5
& 👉 tinyurl.com/2vp8p8uk
Very grateful Igor Bonacossa Pereira, Dat Le & Massimo Hilliard @hilliardlab.bsky.social for their creativity & passion.
Read more about it 👉
tinyurl.com/56awjda5
& 👉 tinyurl.com/2vp8p8uk
An epidermal membrane-associated periodic skeleton restricts endocytosis to stabilize neuron-epidermal attachment and preserve axons
An epidermal membrane periodic scaffold protects axons from damage.
tinyurl.com
January 8, 2026 at 2:02 AM
Latest paper is now out and features on the cover of Science Advances 👉 tinyurl.com/vea5862x
Very grateful Igor Bonacossa Pereira, Dat Le & Massimo Hilliard @hilliardlab.bsky.social for their creativity & passion.
Read more about it 👉
tinyurl.com/56awjda5
& 👉 tinyurl.com/2vp8p8uk
Very grateful Igor Bonacossa Pereira, Dat Le & Massimo Hilliard @hilliardlab.bsky.social for their creativity & passion.
Read more about it 👉
tinyurl.com/56awjda5
& 👉 tinyurl.com/2vp8p8uk
Reposted by Stephane Vassilopoulos
What a great start for 2026 for the EVICAT team!!!
Everything you need to know about extracellular vesicles' recipient cells and how to harness these pathways for therapeutic purposes
Thanks to all co-authors!
bit.ly/3N9InyW
Everything you need to know about extracellular vesicles' recipient cells and how to harness these pathways for therapeutic purposes
Thanks to all co-authors!
bit.ly/3N9InyW
Biology and therapeutic potential of extracellular vesicle targeting and uptake - Nature Reviews Molecular Cell Biology
Extracellular vesicles are released from almost all cell types and mediate intercellular communication by delivering proteins, nucleic acids and lipids. This Review examines extracellular vesicle upta...
bit.ly
January 4, 2026 at 7:20 PM
What a great start for 2026 for the EVICAT team!!!
Everything you need to know about extracellular vesicles' recipient cells and how to harness these pathways for therapeutic purposes
Thanks to all co-authors!
bit.ly/3N9InyW
Everything you need to know about extracellular vesicles' recipient cells and how to harness these pathways for therapeutic purposes
Thanks to all co-authors!
bit.ly/3N9InyW
Reposted by Stephane Vassilopoulos
A tribute to Stuart Kornfeld, pioneering #glycotime scientist whose work on mannose-6-phosphate receptor lysosomal trafficking (among many other contributions) was highly influential to our work 🫡
www.jci.org/articles/vie...
www.jci.org/articles/vie...
JCI -
A tribute to Stuart A. Kornfeld (1936–2025)
www.jci.org
January 3, 2026 at 3:08 AM
A tribute to Stuart Kornfeld, pioneering #glycotime scientist whose work on mannose-6-phosphate receptor lysosomal trafficking (among many other contributions) was highly influential to our work 🫡
www.jci.org/articles/vie...
www.jci.org/articles/vie...
Reposted by Stephane Vassilopoulos
New paper out!
We show how mechanosensitive adherens junction proteins link actomyosin contractility to actin assembly using in vitro reconstitution.
Huge congrats to Aurélie Favarin, Rayan Said, & all authors!
In Science Advances: www.science.org/doi/10.1126/...
#actin #myosin #mechanobiology
We show how mechanosensitive adherens junction proteins link actomyosin contractility to actin assembly using in vitro reconstitution.
Huge congrats to Aurélie Favarin, Rayan Said, & all authors!
In Science Advances: www.science.org/doi/10.1126/...
#actin #myosin #mechanobiology
January 2, 2026 at 4:00 PM
New paper out!
We show how mechanosensitive adherens junction proteins link actomyosin contractility to actin assembly using in vitro reconstitution.
Huge congrats to Aurélie Favarin, Rayan Said, & all authors!
In Science Advances: www.science.org/doi/10.1126/...
#actin #myosin #mechanobiology
We show how mechanosensitive adherens junction proteins link actomyosin contractility to actin assembly using in vitro reconstitution.
Huge congrats to Aurélie Favarin, Rayan Said, & all authors!
In Science Advances: www.science.org/doi/10.1126/...
#actin #myosin #mechanobiology
Reposted by Stephane Vassilopoulos
Optogenetic SH3 nanoclustering of SRC and HCK uncovers the functional specificity of these redundant kinases in macrophages. https://www.biorxiv.org/content/10.64898/2025.12.27.692121v1
December 28, 2025 at 5:30 AM
Optogenetic SH3 nanoclustering of SRC and HCK uncovers the functional specificity of these redundant kinases in macrophages. https://www.biorxiv.org/content/10.64898/2025.12.27.692121v1
Reposted by Stephane Vassilopoulos
A comprehensive article for our unique Live-to-HPF CLEM workflow in Scientific Reports!🔬❄️ Thanks to all who supported our long journey!
Images are taken @ImagoSeine @ijmonod.bsky.social @imagerie-gif.bsky.social @i2bcparissaclay.bsky.social @jeoleurope.bsky.social
www.nature.com/articles/s41...
Images are taken @ImagoSeine @ijmonod.bsky.social @imagerie-gif.bsky.social @i2bcparissaclay.bsky.social @jeoleurope.bsky.social
www.nature.com/articles/s41...
Combining the CryoCapsule, HPM live µ and R221 conductive resin to track endosome from live imaging to electron microscopy using high-pressure freezing - Scientific Reports
Scientific Reports - Combining the CryoCapsule, HPM live µ and R221 conductive resin to track endosome from live imaging to electron microscopy using high-pressure freezing
www.nature.com
December 19, 2025 at 2:24 PM
A comprehensive article for our unique Live-to-HPF CLEM workflow in Scientific Reports!🔬❄️ Thanks to all who supported our long journey!
Images are taken @ImagoSeine @ijmonod.bsky.social @imagerie-gif.bsky.social @i2bcparissaclay.bsky.social @jeoleurope.bsky.social
www.nature.com/articles/s41...
Images are taken @ImagoSeine @ijmonod.bsky.social @imagerie-gif.bsky.social @i2bcparissaclay.bsky.social @jeoleurope.bsky.social
www.nature.com/articles/s41...
Reposted by Stephane Vassilopoulos
@natcellbio.nature.com - Finally out! Skin cells mechanically protect their genome from UV by positioning melanin above nucleus. Keratin filaments & microtubules drive the process. Led by Silvia Benito-Martinez & @laurasalavessa.bsky.social @membramics-lab.bsky.social www.nature.com/articles/s41...
Keratin intermediate filaments mechanically position melanin pigments for genome photoprotection - Nature Cell Biology
Benito-Martínez, Salavessa and colleagues show that keratin intermediate filaments and microtubules control the three-dimensional perinuclear position of melanin-containing organelles, shielding the D...
www.nature.com
December 15, 2025 at 6:17 PM
@natcellbio.nature.com - Finally out! Skin cells mechanically protect their genome from UV by positioning melanin above nucleus. Keratin filaments & microtubules drive the process. Led by Silvia Benito-Martinez & @laurasalavessa.bsky.social @membramics-lab.bsky.social www.nature.com/articles/s41...
Reposted by Stephane Vassilopoulos
#LivingArchitectures
We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay.
www.musee-orsay.fr/fr/agenda/ev...
Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night.
-> Two shows on the 24th and 25th of January.
We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay.
www.musee-orsay.fr/fr/agenda/ev...
Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night.
-> Two shows on the 24th and 25th of January.
December 5, 2025 at 4:11 PM
#LivingArchitectures
We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay.
www.musee-orsay.fr/fr/agenda/ev...
Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night.
-> Two shows on the 24th and 25th of January.
We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay.
www.musee-orsay.fr/fr/agenda/ev...
Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night.
-> Two shows on the 24th and 25th of January.
Reposted by Stephane Vassilopoulos
Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
December 2, 2025 at 9:02 PM
Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
Reposted by Stephane Vassilopoulos
Great collaborative work led by Christopher Toepfer and his team at the University of Oxford
Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery - Nature Protocols
The Mant-ATP assay is a simple and accessible means for rapid quantitative assessment of the ratio of super-relaxed myosin to disordered relaxed myosin. This protocol provides a standardized methodolo...
www.nature.com
December 1, 2025 at 4:21 PM
Great collaborative work led by Christopher Toepfer and his team at the University of Oxford
Reposted by Stephane Vassilopoulos
is there a direct crosstalk between actin and vimentin intermediate filaments? Our work shows that vimentin promotes actin assembly by stabilizing ATP-subunits at the barbed end.
Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social
www.biorxiv.org/content/10.1...
Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social
www.biorxiv.org/content/10.1...
November 24, 2025 at 10:15 AM
is there a direct crosstalk between actin and vimentin intermediate filaments? Our work shows that vimentin promotes actin assembly by stabilizing ATP-subunits at the barbed end.
Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social
www.biorxiv.org/content/10.1...
Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social
www.biorxiv.org/content/10.1...
Reposted by Stephane Vassilopoulos
I am thrilled to share our new article entitled 'Alternative splicing of SORBS1 affects neuromuscular junction integrity in myotonic dystrophy type 1 onlinelibrary.wiley.com/doi/10.1002/...
@cecilemartinat.bsky.social
@cecilemartinat.bsky.social
Alternative Splicing of SORBS1 Affects Neuromuscular Junction Integrity in Myotonic Dystrophy Type 1
Background Myotonic dystrophy type 1 (DM1) is a multisystemic neuromuscular disorder characterized by CTG repeat expansion in the 3′ untranslated region of the dystrophia myotonica protein kinase co...
onlinelibrary.wiley.com
November 21, 2025 at 10:11 AM
I am thrilled to share our new article entitled 'Alternative splicing of SORBS1 affects neuromuscular junction integrity in myotonic dystrophy type 1 onlinelibrary.wiley.com/doi/10.1002/...
@cecilemartinat.bsky.social
@cecilemartinat.bsky.social
Reposted by Stephane Vassilopoulos
PhD student management tip from his advisor René Couteaux, discoverer of the neuromuscular junction: "*points at 50 cm of lab bench* here's where you'll work. I'll be back in three months to see what you've done"
November 19, 2025 at 7:18 PM
PhD student management tip from his advisor René Couteaux, discoverer of the neuromuscular junction: "*points at 50 cm of lab bench* here's where you'll work. I'll be back in three months to see what you've done"
Reposted by Stephane Vassilopoulos
I'm at the French Society for Myology meeting with @stefvass.bsky.social! He just made a book about the late Michel Fardeau, first president of the society, pioneer of electron microscopy for muscle and muscle disease
en.wikipedia.org/wiki/Michel_...
en.wikipedia.org/wiki/Michel_...
November 19, 2025 at 7:15 PM
I'm at the French Society for Myology meeting with @stefvass.bsky.social! He just made a book about the late Michel Fardeau, first president of the society, pioneer of electron microscopy for muscle and muscle disease
en.wikipedia.org/wiki/Michel_...
en.wikipedia.org/wiki/Michel_...
Reposted by Stephane Vassilopoulos
Want to hear a mid-career PI pontificate about science, career and cooking? Today's your lucky day thanks to The Microscopists podcast! Thanks Peter @yorkbioimaging.bsky.social for having me 🎙️🎧
themicroscopists.bitesizebio.com/episodes/chr...
themicroscopists.bitesizebio.com/episodes/chr...
The Microscopists | Christophe Leterrier (CNRS & Aix-Marseille Université)
This time on The Microscopists, we're joined by Christophe Leterrier, team leader at NeuroCyto in Marseille and director of a Nikon Center of Excellence for super-resolution microscopy.Christophe r...
themicroscopists.bitesizebio.com
October 3, 2025 at 12:40 PM
Want to hear a mid-career PI pontificate about science, career and cooking? Today's your lucky day thanks to The Microscopists podcast! Thanks Peter @yorkbioimaging.bsky.social for having me 🎙️🎧
themicroscopists.bitesizebio.com/episodes/chr...
themicroscopists.bitesizebio.com/episodes/chr...
Reposted by Stephane Vassilopoulos
New NeuroCyto preprint about to drop! @wiesner-t.bsky.social 😎⏳
September 16, 2025 at 4:48 PM
New NeuroCyto preprint about to drop! @wiesner-t.bsky.social 😎⏳
Reposted by Stephane Vassilopoulos
www.sciencedirect.com/science/arti...
check out the recent dispatch on endolysosomes and melano-biology interplay in @currentbiology.bsky.social by @delevoyecedric.bsky.social & @laurasalavessa.bsky.social from @membramics-lab.bsky.social
check out the recent dispatch on endolysosomes and melano-biology interplay in @currentbiology.bsky.social by @delevoyecedric.bsky.social & @laurasalavessa.bsky.social from @membramics-lab.bsky.social
September 8, 2025 at 3:48 PM
www.sciencedirect.com/science/arti...
check out the recent dispatch on endolysosomes and melano-biology interplay in @currentbiology.bsky.social by @delevoyecedric.bsky.social & @laurasalavessa.bsky.social from @membramics-lab.bsky.social
check out the recent dispatch on endolysosomes and melano-biology interplay in @currentbiology.bsky.social by @delevoyecedric.bsky.social & @laurasalavessa.bsky.social from @membramics-lab.bsky.social
Reposted by Stephane Vassilopoulos
If you're interested in trafficking of #myelin constituents in #oligodendrocytes, and of course you are, you may want to check out our new preprint. It's very nice PhD work by @sophiesiems.bsky.social, and we're grateful to all who contributed!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Adaptor protein complex 2 (AP2) participates in biogenesis and homeostasis of myelin sheaths in the central nervous system
Myelination of CNS axons requires oligodendrocytes to undergo extensive morphological changes by producing large amounts of myelin membrane with defined protein composition and structure. The formatio...
www.biorxiv.org
September 3, 2025 at 11:51 AM
If you're interested in trafficking of #myelin constituents in #oligodendrocytes, and of course you are, you may want to check out our new preprint. It's very nice PhD work by @sophiesiems.bsky.social, and we're grateful to all who contributed!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Stephane Vassilopoulos
Happy to contribute to this new work from @micoxscopy.bsky.social et al, helping apply SSQUASH to analyse the shape of spectrin rings along axons. Data analysis is the next frontier in SMLM, and I'm always up for chatting, sharing data and see how we can push things further!
August 13, 2025 at 10:38 AM
Happy to contribute to this new work from @micoxscopy.bsky.social et al, helping apply SSQUASH to analyse the shape of spectrin rings along axons. Data analysis is the next frontier in SMLM, and I'm always up for chatting, sharing data and see how we can push things further!
Reposted by Stephane Vassilopoulos
Hello microscopy and imaging experts and enthusiasts!
We are looking for a staff scientist to join us here in Geneva to work on microscopy and image analysis projects! See the link for the job announcement (in French and in English).
Please apply and/or repost!
jobs.unige.ch/www/wd_porta...
We are looking for a staff scientist to join us here in Geneva to work on microscopy and image analysis projects! See the link for the job announcement (in French and in English).
Please apply and/or repost!
jobs.unige.ch/www/wd_porta...
July 7, 2025 at 2:05 PM
Hello microscopy and imaging experts and enthusiasts!
We are looking for a staff scientist to join us here in Geneva to work on microscopy and image analysis projects! See the link for the job announcement (in French and in English).
Please apply and/or repost!
jobs.unige.ch/www/wd_porta...
We are looking for a staff scientist to join us here in Geneva to work on microscopy and image analysis projects! See the link for the job announcement (in French and in English).
Please apply and/or repost!
jobs.unige.ch/www/wd_porta...
Reposted by Stephane Vassilopoulos
How do cells switch on the actin assembly at endocytic sites? How can this on-switch evolve? Check out our new preprint!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
June 24, 2025 at 7:22 PM
How do cells switch on the actin assembly at endocytic sites? How can this on-switch evolve? Check out our new preprint!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...