Lightnews — Scholar-powered news

Alessandro Vannini

@alessandrovannini.bsky.social

970 followers 680 following 4 posts

Head of Structural Biology Research Centre of Human Technopole, Milan Professor of Integrative Structural Biology. Our Team works on #RNAPolymeraseIII #SMC complexes and associated factors in genome function/organisation and viral sensing

Posts Media Videos Starter Packs

Alessandro Vannini @alessandrovannini.bsky.social · Jul 3

Condensin II activation by M18BP1: Molecular Cell www.cell.com/molecular-ce...

Condensin II activation by M18BP1

Borsellini, Conti, Cutts, Harris, et al. identify an interaction between M18BP1 and condensin II that is essential for condensin II activity and chromatin localization. MCPH1 keeps the interphase geno...

www.cell.com

4 16

Reposted by Alessandro Vannini

Alexey Amunts @amunts.bsky.social · Mar 15

See you in Madrid! Speakers include:
@costalaboratory.bsky.social @piratefernandez.bsky.social @nhoitsma.bsky.social @kellythd-nguyen.bsky.social @lapassmore.bsky.social @alessandrovannini.bsky.social @oscarllorca.bsky.social @rfleiro.bsky.social @nogaleslab.bsky.social
www.cnio.es/en/eventos/m...

Machines acting on DNA and RNA, a molecular mechanistic perspective (#CFM_MolMech)

www.cnio.es

4 4

Alessandro Vannini @alessandrovannini.bsky.social · Jan 3

Excited to share the (hard) work of @zawarshah.bsky.social in its final form: www.nature.com/articles/s41...

A lot of insights into RNA Polymerase selectivity at snRNAs promoters !! As these promoters are often used for heterologous expression of RNAs (ie guide RNAs), you better know how they work

Structural insights into distinct mechanisms of RNA polymerase II and III recruitment to snRNA promoters - Nature Communications

The snRNA Activating Protein Complex (SNAPc) mediates snRNA transcription by RNA Pol II and III. Here, the authors have used cryo-EM to resolve the structure of the SNAPc-bound RNA Pol III pre-initiat...

www.nature.com

4 19 52

Reposted by Alessandro Vannini

Sebastiano Pasqualato @spasqualato.bsky.social · Nov 20

. @zawarshah.bsky.social and @nikklena.bsky.social killing it at the Milan Joint Structural Biology Lab Meeting! Great afternoon gathering the Attu bio community in #uniMI
Amazing showcase of @humantechnopole.bsky.social science from @alessandrovannini.bsky.social and @gaiapigino.bsky.social lab

1 2 11

Reposted by Alessandro Vannini

Matthew Freeman @mjafreeman.bsky.social · Nov 19

BlueSky has undergone a Cambrian explosion in the last week!

>20M users today: I can’t keep up with all the starter packs

I will continue to repost those I see...complete with collective nouns

But no pretence of being comprehensive

1/2

2 5 40

Reposted by Alessandro Vannini

Oliver Harschnitz @harschnitz.bsky.social · Nov 20

For anything stem cell and pluripotency-related: go.bsky.app/UTW4d7b

1 9 13

Reposted by Alessandro Vannini

Teif lab @teiflab.bsky.social · Nov 16

Interesting new method to map nucleosomes in 3D, "Proximity Copy Paste (PCP)". www.biorxiv.org/content/10.1...
▶️ mapped connectivity of nucleosomes in S. cerevisiae
▶️ chromatin is predominantly organized into regularly spaced nucleosome arrays
▶️ metaphase chromosomes packed by arrayed cohesin hubs

PCP maps 3D genome organization at the nucleosome level.
(a) Schematic of the PCP reaction on chromatin. Crosslinked chromatin is digested into nucleosomes by Caspase Activated DNAse (CAD) and then immobilized onto magnetic beads. Digested chromatin is ligated by seed and receptor molecules to the ratio of 1/10. The PCP reaction takes place on the beads where RNA tags will diffuse from the seeds and tag nearby receptors. The chromatin is then decrosslinked, the proteins and RNA removed, and a sequencing library generated by PCR. Molecules sharing the same UMI are grouped. Tag groups can be analyzed in several ways, including individual multiway tag groups or pairwise interaction matrices. (b) Plot showing the relative frequency of receptor tagging as a function of distance from the seed, the midpoints of the tagged molecules is shown. Seeds that self-tag are excluded from this analysis. (c) Plot showing the number of reads per seed tag groups for G1 data. (d) Distance decay interaction plots of PCP (orange) and Micro-C (Costantinto et al. 2020, 15 min 23°C sample) (res = 50bp).

1 15 40

Reposted by Alessandro Vannini

Mark Peifer (He, him) @peiferlabunc.bsky.social · Nov 14

This is mind blowing! Sequencing DNA INSIDE single nuclei to map the 3D position of genes. I love the combination of technologies--imaging powered by expansion microscopy and state-of-the art sequencing, all underlain by good old Fred Sanger 🧪

zack chiang @zchiang.bsky.social · Nov 11

7 years ago, I met a junior fellow named Jason Buenrostro who blew me away with a vision of futuristic genomic technologies

Today, we (Ajay Labade, Caroline Comenho) are excited to share our first steps into that future: Expansion in situ genome sequencing

1/

15 88

Alessandro Vannini @alessandrovannini.bsky.social · Nov 14

Woahhhhhhhh ! Congratulations

1 1

Reposted by Alessandro Vannini

Karim-Jean Armache @kjarmache.bsky.social · Nov 13

Please see our paper in Nature on read-write mechanisms of H2AK119 ubiquitination by Polycomb repressive complex I. Congrats to the whole team, especially Victoria and huge thanks to our collaborator JP Armache! Also big thanks to Mark Foundation for Cancer Research for the support! rdcu.be/dZ5HZ

Read–write mechanisms of H2A ubiquitination by Polycomb repressive complex 1

Nature - Cryo-electron microscopy and biochemical studies elucidate the read–write mechanisms of non-canonical PRC1-containing RYBP in histone H2A lysine 119 monoubiquitination and their...

rdcu.be

7 31 120

Reposted by Alessandro Vannini

Rémi Fronzes @fronzeslab.bsky.social · Nov 13

FYI: I am trying to add people who post to the #cryoEM and #cryoET feeds to the CryoEM/ET starter pack.

7 20

Alessandro Vannini @alessandrovannini.bsky.social · Nov 13

Let’s give this platform a good go and hopefully say goodbye 👋 to the birdie one

1 3 10