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Reposted by Alexandre Dumoulin

Victor Tatarskiy @greenrat.bsky.social · 6d

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

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Alexandre Dumoulin @axonsalex.bsky.social · 9d

Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪

Spirochrome @spirochrome.com · 13d

Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition!

His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.

www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday

In vitro growth of chick sensory neurons | 2025 Small World in Motion Competition

Alexandre Dumoulin - In vitro growth of chick sensory neurons, shown in gold using a dye marking their internal structure (18 hour time lapse)

www.nikonsmallworld.com

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Alexandre Dumoulin @axonsalex.bsky.social · Aug 5

very nice interview Joaquín!

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Reposted by Alexandre Dumoulin

Zhixing Chen @zhixingchen2.bsky.social · Jul 16

Check it out at PNAS today. Thank you Alexandre for the collaboration!!

PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!

Available @spirochrome.com

Alexandre Dumoulin @axonsalex.bsky.social · Jul 16

Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social !
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001

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Alexandre Dumoulin @axonsalex.bsky.social · Jul 16

Thanks Luc! and yes it is! 😉

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Alexandre Dumoulin @axonsalex.bsky.social · Jul 16

Here’s an embryonic axonal growth cone labeled with PK Mem dye (red) and tubulin (blue), imaged every 2 seconds. 🤩

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Alexandre Dumoulin @axonsalex.bsky.social · Jul 16

Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social !
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001

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Reposted by Alexandre Dumoulin

Bassem Hassan @bassemh.bsky.social · May 7

Hot off the press:
The excellent Maheva Andriatsilavo and colleagues show how a temporal sequence of stochastic molecular mechanisms allow the emergence of stereotyped individualised neuronal circuits. Just published in Nature Neuroscience:
rdcu.be/ek010

Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster

Nature Neuroscience - The developmental origin of behavioral individuality is unclear. The authors show that a temporal sequence of genetically encoded stochastic mechanisms explains variation in...

rdcu.be

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Reposted by Alexandre Dumoulin

The Hassan Brain Development Team @hassanbraindevlab.bsky.social · May 7

New from the lab: @maheandria.bsky.social and colleagues in collaboration with the Hiesinger, Stoeckli and von Kleist labs dissect the sequential emergence of axonal targeting during development in today's issue of Nature Neuroscience:
rdcu.be/ek010

Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster

Nature Neuroscience - The developmental origin of behavioral individuality is unclear. The authors show that a temporal sequence of genetically encoded stochastic mechanisms explains variation in...

rdcu.be

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Alexandre Dumoulin @axonsalex.bsky.social · May 2

cool!
here are chick commissural axons crossing the neural tube floor plate :): bsky.app/profile/axon...

Alexandre Dumoulin @axonsalex.bsky.social · Nov 13

May commissural axons find their way in here 🙏
#Microscopy #LiveImaging #AxonGuidance #Neuroscience

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Alexandre Dumoulin @axonsalex.bsky.social · May 2

Hi Erica,
thanks!
for 2D cultures: journals.biologists.com/jcs/article/...
for 3D cultures: www.frontiersin.org/journals/mol...
for live imaging I used 8-well IBIDI μ-slides with same PLL/Laminin coating and the same medium as in the JCS paper.
Cheers
Alex

The SynCAM synaptic cell adhesion molecules are involved in sensory axon pathfinding by regulating axon–axon contacts

ABSTRACT. Synaptic cell adhesion molecules (SynCAMs) are crucial for synapse formation and plasticity. However, we have previously demonstrated that SynCAMs are also required during earlier stages of ...

journals.biologists.com

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Reposted by Alexandre Dumoulin

Kevin Mitchell @wiringthebrain.bsky.social · Apr 30

These ones are growing in a dish, but in a developing embryo or brain, each growth cone would be sniffing its way through a complex molecular terrain, often over long distances to find its correct synaptic partners.

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Alexandre Dumoulin @axonsalex.bsky.social · Apr 30

Hi Luc, thanks!
it is indeed Spy555-FastAct.
Cheers

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Alexandre Dumoulin @axonsalex.bsky.social · Apr 30

it is a filopodial (thin protusions) party in there! 🕺🧐
🧪 #liveimaging

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Alexandre Dumoulin @axonsalex.bsky.social · Apr 30

This time-lapse captures 17 hours of axonal growth from a chicken dorsal root ganglion explant, visualized through the actin cytoskeleton using live confocal imaging.
I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉
🧪

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Reposted by Alexandre Dumoulin

Nikita Vladimirov @nvladimus.bsky.social · Jan 20

Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!

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Alexandre Dumoulin @axonsalex.bsky.social · Jan 10

me too! They seem to be retraction fibers that are then fragmenting into these small vesicles. It has been described in some migrating cells/cancer cells before. We are now inestigating it in the context of axon growth and guidance!

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Alexandre Dumoulin @axonsalex.bsky.social · Jan 10

Yes, it is, I wrote it in the ALT text but somehow it I can't see it. SPY555-FastAct

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Alexandre Dumoulin @axonsalex.bsky.social · Jan 10

And sometimes accross the floor plate 🙂

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Alexandre Dumoulin @axonsalex.bsky.social · Jan 10

🤩

Reposted by Alexandre Dumoulin

Michael Way @drmichaelway.bsky.social · Jan 6

A brief history of The Company of Biologists - 100 years of supporting scientists!👍👏
journals.biologists.com/jcs/article/...

The Company of Biologists: celebrating 100 years

Summary: As we begin celebrating our 100-year anniversary, we reflect on our extraordinary story so far, consider the Company's current support for our communities and prepare for some inspiring activ...

journals.biologists.com

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Reposted by Alexandre Dumoulin

Roberto Mayor Lab @mayorlab.bsky.social · Jan 4

Join us in beautiful Quintay to celebrate 25 years of the Practical Course on Developmental Biology!
To register go to biodesarrollo.unab.cl/mini-symposi...
Come to enjoy a unique blend of science, sea, and food – all for free!

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Reposted by Alexandre Dumoulin

Addgene @addgene.bsky.social · Dec 31

Did you know 2024 was our 20th anniversary? We're closing it out by sharing a brief history of Addgene, with commentary from our incredible community.
Read it below!

blog.addgene.org/add...

Addgene: The First Twenty Years

Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.

blog.addgene.org

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Reposted by Alexandre Dumoulin

Danielle Beckman @daniellebeckman.bsky.social · Dec 27

2024 #FluorescenceFriday retrospective: It was a great year in science for microglia, the brain's immune cells! The brain is much more than neurons; these little cells are essential, particularly in inflammatory processes. Check them interacting below! Markers: neurons (🔵 MAP2), microglia (🟡 IBA1)

Big blue neurons surrounded by small yellow microglia everywhere!

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Reposted by Alexandre Dumoulin

Zhixing Chen @zhixingchen2.bsky.social · Dec 19

How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling.
www.biorxiv.org/content/10.1...

Special thanks to @aliceyting.bsky.social for discussions

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