Senescent OPCs, caused by a declined autophagy, suppress neuronal firing and excitatory transmission via CCL3/5-CCR5 signaling.

Steric hindrance affects the relocation of ER-to-LD proteins and primarily regulates the LD proteome.

Ohnishi et al. present the AVID mouse, which enables real-time, multiscale imaging of ATP dynamics in vivo across organs and cell types. This platform reveals spatially heterogeneous changes in energy metabolism during both physiological conditions and pathological states such as myocardial infarction, enabling systems-level analysis of metabolic regulation.

Move could signal return to ban on such studies imposed by first Trump administration

An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...

Blastoids are stem cell-derived structures that mimic natural blastocysts by incorporating all three lineages: trophectoderm, epiblast, and primitive endoderm. However, current methods often yield inc...

ATG9A is transmembrane autophagic machinery protein that delivers phospholipids to expanding autophagosomes. Mailler et al. show that ATG9A is required to mobilize lipids from lipid droplets for autop...

The autophagy-related protein ATG9A is integral to cellular autophagy and lipid mobilization, yet its importance in mammalian physiology remains underexplored. Using a liver-specific conditional At...

Individual proteins tend to adopt preferred orientations when subjected to vitrification for cryo-electron microscopy analysis. A laser flash melting procedure followed by rapid revitrification provid...

Losier et al. identify distinct kinase signaling pathways that differentially regulate bulk and selective autophagy. Using kinome-wide CRISPR screening, th

Ma et al. reveal that lipolysis is controlled by both lipase activity (biochemistry) and lipase-accessible lipid droplet surface area (biophysics). The derivative of the linear relationship between lipolytic flux and lipid droplet surface area is called lipolytic efficiency, which is equivalent to the lipase activity.

Gao, Li and colleagues derive trophectoderm stem cells from 32-cell mouse embryos. These cells represent an early trophectoderm state and are capable of developing into placenta cells, forming placent...

Ch-TS cells may serve as a valuable in vitro model for studying trophoblast biology and placenta-mediated pregnancy complications, similar to TS^CT^/blast cells.