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emgoodall.bsky.social
Emily Goodall
@emgoodall.bsky.social
120 followers 160 following 9 posts
Microbiologist - Bacterial genetics
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · Aug 14
Out yesterday. Using a translation reporter on a mini-tn5 transposon, and high freq. mutagenesis, we looked at translation on whole genome scale independently of genome annotation. academic.oup.com/nar/article/...
PIRT-Seq: a high-resolution whole-genome assay to identify protein-coding genes
Abstract. The advent of high-density mutagenesis and data-mining studies suggest the existence of further coding potential within bacterial genomes. Small
academic.oup.com
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · Aug 14
Thank you @narjournal.bsky.social and reviewers, and thank you team @hendersonlab.bsky.social @weinehue.bsky.social Brian forde and Matt Hemm, and Jack Bryant who was there from the beginning
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · Aug 14
I think there's a LOT more that can be done through transposon mutagenesis to query a genome through editing your transposon!
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · Aug 14
Like all high throughout screens we got some unusual results too, the biology of which is still to be explored ('nested' genes??)
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · Aug 14
Some of these small genes are conserved and/or neighbouring well chracterised genes. Highlights that small genes/proteins are still overlooked.
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · Aug 14
Clearly more unannotated coding potential within E. coli. Interesting to see what different methods do and don't identify.
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · Aug 14
We did this in E. coli and identified some new small proteins, validated by western blot.
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · Aug 14
Out yesterday. Using a translation reporter on a mini-tn5 transposon, and high freq. mutagenesis, we looked at translation on whole genome scale independently of genome annotation. academic.oup.com/nar/article/...
PIRT-Seq: a high-resolution whole-genome assay to identify protein-coding genes
Abstract. The advent of high-density mutagenesis and data-mining studies suggest the existence of further coding potential within bacterial genomes. Small
academic.oup.com
4 16 33
Reposted by Emily Goodall
annaolina.bsky.social
Anna Olina @annaolina.bsky.social · Jun 24
Happily representing @multidefence.bsky.social at the #EESMicrobiology in Heidelberg!
Come to my poster if interested in prophage-mediated phage defence!
Also don't miss @emgoodall.bsky.social's talk today, and @alekseiagapov.bsky.social and @davvi36.bsky.social posters!
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Reposted by Emily Goodall
davvi36.bsky.social
David Sünderhauf @davvi36.bsky.social · Jun 24
Made it to Heidelberg for #EESMicrobiology @events.embl.org!
Find me at poster 240 and make to check out @annaolina.bsky.social & @alekseiagapov.bsky.social 's posters and @emgoodall.bsky.social 's talk to see everyone from the lab 😁
Me showing off my poster
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · May 21
Home sweet home! After an excellent time with @hendersonlab.bsky.social in Aus! I have finally returned to the UK and started a postdoc position this week with the van Houte lab at @uniofexeteresi.bsky.social with some fun research to come
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Reposted by Emily Goodall
proftracypalmer.bsky.social
Tracy Palmer @proftracypalmer.bsky.social · Apr 9
Two ERC-funded postdoc positions available in the team - one here and second to follow - please repost! jobs.ncl.ac.uk/job/Newcastl...
Research Associate Genetic and functional analysis of Gram positive secretion systems and substrates
Research Associate Genetic and functional analysis of Gram positive secretion systems and substrates
jobs.ncl.ac.uk
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Reposted by Emily Goodall
jgreener64.bsky.social
Joe Greener @jgreener64.bsky.social · Apr 8
This has been a long journey, but it's now out in final form. @kjamali.bsky.social

academic.oup.com/bioinformati...
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Reposted by Emily Goodall
plosbiology.org
PLOS Biology @plosbiology.org · Apr 8
The BASEL collection of E. coli #phages is limited by its host strain. @humollidorentina.bsky.social @damienpiel.bsky.social @aharms485.bsky.social &co use a modified host to add 36 new phages, revealing roles of O-antigen in recognition & R-M systems in immunity @plosbiology.org 🧪 plos.io/4jirGvX
A fanciful depiction of BASEL phages on a roadmap. Credit: Fabienne Estermann
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Reposted by Emily Goodall
natmicrobiol.nature.com
Nature Microbiology @natmicrobiol.nature.com · Mar 27
OUT NOW: Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice

#microsky 🧪

www.nature.com/articles/s41...
Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice - Nature Microbiology
InducTn-seq, a method for inducible mutagenesis followed by transposon insertion site sequencing, enables temporal control of transposition to bypass population bottlenecks and enable the quantificati...
www.nature.com
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Reposted by Emily Goodall
natrevmicro.nature.com
Nature Reviews Microbiology @natrevmicro.nature.com · Mar 27
New online! Genetics, ecology and evolution of phage satellites
Genetics, ecology and evolution of phage satellites
Nature Reviews Microbiology, Published online: 27 March 2025; doi:10.1038/s41579-025-01156-zIn this Review, Penadés et al. explore the genetics, potential origins and life cycle of phage satellites, and they discuss the impact of these elements on the evolution of other mobile genetic elements and their host bacteria.
www.nature.com
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emgoodall.bsky.social
Emily Goodall @emgoodall.bsky.social · Mar 26
Currently nap trapped, seemed like a good opportunity to finally set this up. Hellooooo science friends 👋
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