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Ryan Flynn @raflynn5.bsky.social · 1h

Spanning a lot of biology and technologies our collaborators were immensely helpful - key contributions from the labs of Kim Simpfendorfer @lozanzi.bsky.social @lmoeckl.bsky.social @axexie.bsky.social @garcialabms.bsky.social and Suneet Agarwal

Ryan Flynn @raflynn5.bsky.social · 1h

Thanks to you and Vale for all the help so far!

Reposted by Ryan Flynn

Ivan Zanoni @lozanzi.bsky.social · 1h

Exciting #science as always @raflynn5.bsky.social &friends 👇👇👇 Thrilled to be part of this 🤗

Ryan Flynn @raflynn5.bsky.social · 1d

We’re excited to report work led by postdoc Jennifer Porat in the lab, finding that DNA accumulates on the surface of living cells and that the secreted extracellular protein DNASE1L3 can modulate its levels on B and T cells. With a new twist for ATAC-seq as well www.biorxiv.org/content/10.1...

DNASE1L3 surveils mitochondrial DNA on the surface of distinct mammalian cells

The extracellular space is a critical environment for discriminating self versus non-self nucleic acids and initiating the appropriate immune responses through signaling cascades to relay information ...

www.biorxiv.org

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Ryan Flynn @raflynn5.bsky.social · 2h

Thanks Leo

Reposted by Ryan Flynn

C&EN (Chemical & Engineering News) @cenmag.bsky.social · 3h

The 2025 #NobelPrize in Chemistry has been awarded to Susumu Kitagawa, Richard Robson and Omar M. Yaghi “for the development of metal–organic frameworks.” Stay tuned for the full story to come! cen.acs.org/people/nobel...

#ChemNobel #Chem #Chemistry #chemsky 🧪

The 2025 chemistry Nobel goes to MOFs

Susumu Kitagawa, Richard Robson and Omar M. Yaghi win the prize for developing metal–organic frameworks

cen.acs.org

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Reposted by Ryan Flynn

Timothy Bredy @twbredy.bsky.social · 10h

Say what? 👍

Ryan Flynn @raflynn5.bsky.social · 1d

We’re excited to report work led by postdoc Jennifer Porat in the lab, finding that DNA accumulates on the surface of living cells and that the secreted extracellular protein DNASE1L3 can modulate its levels on B and T cells. With a new twist for ATAC-seq as well www.biorxiv.org/content/10.1...

DNASE1L3 surveils mitochondrial DNA on the surface of distinct mammalian cells

The extracellular space is a critical environment for discriminating self versus non-self nucleic acids and initiating the appropriate immune responses through signaling cascades to relay information ...

www.biorxiv.org

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Reposted by Ryan Flynn

Ben Schumann @drglycoben.bsky.social · 1d

Check out our new preprint "Convergent Lead Discovery Strategies Yield Covalent and Non-covalent Inhibitors of Human UDP-Galactose-4-Epimerase" on Chemrxiv led by Will Browne in close collab with GSK @jtbush.bsky.social

chemrxiv.org/engage/chemr...

Convergent Lead Discovery Strategies Yield Covalent and Non-covalent Inhibitors of Human UDP-Galactose-4-Epimerase

O-GalNAc (N-acetylgalactosaminyl) glycosylation is an abundant posttranslational modification in mammalian cells. Dysregulation of O-GalNAc glycosylation is implicated in cancer metastasis and immune ...

chemrxiv.org

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Reposted by Ryan Flynn

BioF:GREAT @biof-great.bsky.social · 19h

Think DNA and proteins are the whole story? Think again! 🤯 The Flynn lab just found a powerful new way to map out glycoRNAs using their super-sensitive rPAL-seq method. This innovative technique revealed glycoRNA patterns on snRNAs/snoRNAs and extracellular vesicles. #CellBiology #glycotime 🧪

Ryan Flynn @raflynn5.bsky.social · 2d

Lead by postdoc in the lab Ruiqi Ge and in collaboration with Bob Coffey’s lab, we are happy to share rPAL-seq for rapid and sensitive sequencing based profiling of glycoRNAs @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...

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Reposted by Ryan Flynn

Bloomberg News @bloomberg.com · 1d

Business leaders, investors and academics have cheered Massachusetts Governor Maura Healey’s efforts to counter the Trump administration’s research funding cuts with state money — so much so that a recent meeting on the initiative required overflow seating.

Massachusetts Counter to Trump Science Cuts Stalls in Statehouse

Business leaders, investors and academics have cheered Massachusetts Governor Maura Healey’s efforts to counter the Trump administration’s research funding cuts with state money — so much so that a recent meeting on the initiative required overflow seating.

bloom.bg

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Ryan Flynn @raflynn5.bsky.social · 1d

This work was support by NIH NIGMS, NIDDK, Rita Allen Foundation, @damonrunyon.org @maxplanck.bsky.social @dfg.de, and Scleroderma Research Foundation

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Ryan Flynn @raflynn5.bsky.social · 1d

Together, we have added (maybe) the last nucleic acid puzzle piece to the cell surface by finding DNA on select living cells; with an exciting relationship building between csDNA, DNASE1L3, and autoimmunity.

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Ryan Flynn @raflynn5.bsky.social · 1d

Finally, DNASE1L3 has been previously established as regulating dsDNA levels in the plasma and loss can result in auto-Abs to dsDNA. Interestingly, in vitro, DNASE1L3 could cleave csDNA from B cells in patients with inactivating mutations in the enzyme we see csDNA accumulates on B cells and T cells

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Ryan Flynn @raflynn5.bsky.social · 1d

We found that csDNA is not restricted to cultured cells, but in health donors, B cells and monocytes had clear csDNA accumulation while T cells and NK cells did not.

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Ryan Flynn @raflynn5.bsky.social · 1d

Protein proximity labeling and mass spec (pPL-MS) defined the local proteomes of csDNA revealing certain membrane domains enriched in addition to some csRBPs and glycoRNAs.

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Ryan Flynn @raflynn5.bsky.social · 1d

Inhibitor and enzyme treatment experiments demonstrated that healthy mitochondria and heparan sulfate were needed to maintain csDNA on the surface of living cells

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Ryan Flynn @raflynn5.bsky.social · 1d

Wanting a simpler assay to integrate this, we reasoned that the Tn5 enzyme used for ATAC-seq should be able to transpose surface-exposed DNA if applied to intact, living cells. Indeed this was possible and dye-coupled oligos enabled imaging of the csDNA on cells.

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Ryan Flynn @raflynn5.bsky.social · 1d

We first realized DNA (in addition to RNA) could be liberated from the cell surface using trypsin - this DNA was oxidized and when sequenced enriched for mitochondrial reads

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Ryan Flynn @raflynn5.bsky.social · 1d

We’re excited to report work led by postdoc Jennifer Porat in the lab, finding that DNA accumulates on the surface of living cells and that the secreted extracellular protein DNASE1L3 can modulate its levels on B and T cells. With a new twist for ATAC-seq as well www.biorxiv.org/content/10.1...

DNASE1L3 surveils mitochondrial DNA on the surface of distinct mammalian cells

The extracellular space is a critical environment for discriminating self versus non-self nucleic acids and initiating the appropriate immune responses through signaling cascades to relay information ...

www.biorxiv.org

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Reposted by Ryan Flynn

Oded Rechavi @odedrechavi.bsky.social · 1d

Fwd: Fwd: “I hope this email finds you well”

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Ryan Flynn @raflynn5.bsky.social · 1d

Thanks Yiliang

Reposted by Ryan Flynn

👻 the present 🎃 @kaplan.bio · 1d

good to see that the RNA sequencing toolbox now has a sugar detector feature

Ryan Flynn @raflynn5.bsky.social · 2d

Lead by postdoc in the lab Ruiqi Ge and in collaboration with Bob Coffey’s lab, we are happy to share rPAL-seq for rapid and sensitive sequencing based profiling of glycoRNAs @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...

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Reposted by Ryan Flynn

Dr Zeinab Rekad 🔬🧬🧪 @zeinabrekad.bsky.social · 1d

#RNAsky

Ryan Flynn @raflynn5.bsky.social · 2d

Lead by postdoc in the lab Ruiqi Ge and in collaboration with Bob Coffey’s lab, we are happy to share rPAL-seq for rapid and sensitive sequencing based profiling of glycoRNAs @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...

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Ryan Flynn @raflynn5.bsky.social · 2d

This work was enabled by funding from NIH NIGMS, NIDDK, @bwfund.bsky.social, and Rita Allen Foundation

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Ryan Flynn @raflynn5.bsky.social · 2d

The sensitivity of rPAL-seq also enabled us to profile glycoRNAs from cells, extracellular vesicles, and secreted non-vesicle fractions including exomeres and supermeres, with distinct glycoRNA transcripts in each fraction.

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Ryan Flynn @raflynn5.bsky.social · 2d

rPAL-seq was applied to 13 cell lines - showing that unlike the bulk small RNA expression, glycoRNA enrichment was able to classify the cells based on their tissue of origin.

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