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Sheila Teves

@sheilateves.bsky.social

1.9K followers 1.9K following 71 posts

Assistant Professor, Biochemistry Department, Transcription & Chromatin biology, Outdoor enthusiast, Dog lover (otters too) 🇵🇭🇺🇲🇨🇦

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Sheila Teves @sheilateves.bsky.social · Nov 8

Latest research from the Teves lab is now out on Biorxiv!

"Functional divergence of TBP homologs through distinct DNA binding dynamics" biorxiv.org/content/10.1...

Here, we examine how conservation is balanced with diversification throughout evolution, using TBP as a case study.

Functional divergence of TBP homologs through distinct DNA binding dynamics

The TATA box-binding protein (TBP) is an evolutionarily conserved basal transcription factor common in the pre-initiation complex of all three eukaryotic RNA polymerases (RNA Pols). Despite their high...

biorxiv.org

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Sheila Teves @sheilateves.bsky.social · 9h

Thanks! Big shoutout to Hazel Cui who led this study and will defend her PhD this year!

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Sheila Teves @sheilateves.bsky.social · 1d

How does a “universal” transcription factor evolve to do species-specific jobs?
Our new preprint reveals how divergence in TBP’s domains shapes transcriptional specialization across eukaryotes.

Read it here 👉

www.biorxiv.org/content/10.1...

#MolecularEvolution #Transcription

Molecular determinants underlying functional divergence of TBP homologs

The TATA-box binding protein (TBP) is a highly conserved basal transcription factor and a core component of the pre-initiation complex (PIC) for all three eukaryotic RNA polymerases (RNA Pols). Despit...

www.biorxiv.org

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Sheila Teves @sheilateves.bsky.social · Jun 12

Flung some discs, shared some laughs, and capped it off with good food. Summer vibes from the Teves lab!

Sheila Teves @sheilateves.bsky.social · Jun 5

Vancouver showing pride

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Sheila Teves @sheilateves.bsky.social · May 22

So excited to see this work out in
@narjournal.bsky.social

academic.oup.com/nar/article/...

Congratulations to Hazel Cui on outstanding work!

Keep an eye out for part 2 of this project 😎

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Reposted by Sheila Teves

Genetics Society of America @genetics-gsa.bsky.social · May 7

In #GENETICS, @sheilateves.bsky.social et al. study the regulation of Pol III transcription during different stages of heat shock stress in mouse embryonic stem cells, showing that the dynamic regulation & recovery of Pol III may be exclusive to the heat shock response.

Read more: buff.ly/s966iZ1

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Sheila Teves @sheilateves.bsky.social · Apr 5

From what we can measure, it affects all tRNA genes! The recovery from HS is a really interesting question

Sheila Teves @sheilateves.bsky.social · Apr 5

Definitely a great experience with Genetics!

Sheila Teves @sheilateves.bsky.social · Apr 3

So proud of Thomas Nguyen and co-authors on the publication of this manuscript. Now out in Genetics!

See full paper here! academic.oup.com/genetics/adv...

Thank you to the editors of Genetics for effiecient handling, and to the peer reviewers for excellent feedback

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Sheila Teves @sheilateves.bsky.social · Jan 12

Really fun chatting with JP, Enya, and Lawrence!

jp flores (he/him) @jpflores.rbind.io · Jan 8

In this episode, I interviewed Dr. Sheila Teves (@sheilateves.bsky.social) and Dr. Lawrence David (Duke) with a fellow graduate student at Duke, Enya (@erd2.bsky.social)! Words cannot explain how fun it was chatting with other Filipino scientists 😊🇵🇭 #representationmatters

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Sheila Teves @sheilateves.bsky.social · Dec 20

The Teves lab totally glammed it up at the departmental holiday party!

Reposted by Sheila Teves

Judith Kribelbauer @jk-swietek.bsky.social · Dec 16

Finally out! We present EXTRA-seq, a new EXTended Reporter Assay to quantify endogenous enhancer-promoter communication at kb scale!
www.biorxiv.org/content/10.1...
A 🧵about what it can do:
#SynBio #DeepLearning #GeneRegulation

EXTRA-seq: a genome-integrated extended massively parallel reporter assay to quantify enhancer-promoter communication

Precise control of gene expression is essential for cellular function, but the mechanisms by which enhancers communicate with promoters to coordinate this process are not fully understood. While seque...

biorxiv.org

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Sheila Teves @sheilateves.bsky.social · Dec 16

Sunshine and rainbows for the little dude

Reposted by Sheila Teves

Lucas Farnung @lucas.farnunglab.com · Dec 12

Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social!
tinyurl.com/setd2

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Reposted by Sheila Teves

Mike Lee @theleelab.bsky.social · Dec 13

We just posted a preprint about a death mechanism that we find truly surprising.

When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!

preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪

Pol II degradation activates cell death independently from the loss of transcription

Pol II-mediated transcription is essential for eukaryotic life. While loss of transcription is thought to be universally lethal, the associated mechanisms promoting cell death are not yet known. Here,...

www.biorxiv.org

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Reposted by Sheila Teves

FragileNucleosome @fnucleosome.bsky.social · Dec 11

🎄🔔Ho Ho ho!😆 Our final
#FragileNucleosome
seminar of the year! We are excited to host Julia and Nithya, two amazing ECR scientists!
🗓️Don't forget to register: us06web.zoom.us/webinar/regi...

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Reposted by Sheila Teves

Shannon Mattern @shannonmattern.bsky.social · Dec 9

"Women full professors are 19% more likely than men at the same career stage to leave academia, as compared with 6% for women assistant professors and 10% for associates."

Women faculty feel ‘pushed’ from academia by poor workplace climate

The gender gap in faculty attrition worsens after tenure, according to a new study

www.science.org

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Reposted by Sheila Teves

Manuel Mendoza @longchrom.bsky.social · Dec 9

1/ One more preprint before the holidays! In which we find that chromatin compaction scales with cell size from interphase through mitosis. A collaboration from waaay back (see end of thread) with Melike Lakadamyali @melikel.bsky.social and Jerome Solon. A thread: 👇
www.biorxiv.org/content/10.1...

Chromatin Compaction Follows a Power Law Scaling with Cell Size from Interphase Through Mitosis

Coordination of mitotic chromosome compaction with cell size is crucial for proper genome segregation during mitosis. During development, DNA content remains constant but cell size evolves, necessitat...

www.biorxiv.org

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Reposted by Sheila Teves

Scott Rothbart @rothbartlab.bsky.social · Dec 4

Thrilled to share a new study from the lab revealing an H3K18ub-H3K9me3 crosstalk mechanism, roles for UHRF1 and its E3 ligase activity in regulating a hierarchy of repressive histone methylation signaling, and a strategy for targeting SUV39H1/H2 to improve DNMT1 inhibitor efficacy! #epigenetics

DNA hypomethylation promotes UHRF1-and SUV39H1/H2-dependent crosstalk between H3K18ub and H3K9me3 to reinforce heterochromatin states

Liu et al. report that DNMT1 inhibition causes transient accumulation of hemi-methylated DNA at CpG islands, which stimulates UHRF1-mediated H3K18ub. This enhances SUV39H1/H2 activity, nucleates new H...

www.cell.com

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Sheila Teves @sheilateves.bsky.social · Dec 1

🙏

Reposted by Sheila Teves

John Ngo @johntngo.bsky.social · Nov 30

We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs.

You can check out our pre-print here: tinyurl.com/27p7hyt9,
and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...

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Sheila Teves @sheilateves.bsky.social · Nov 30

Yeah, we’ve been using Cut&Tag for TFs for years now… seems fine for some but not others 🤷‍♀️

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Sheila Teves @sheilateves.bsky.social · Nov 30

🙏

Reposted by Sheila Teves

Fyodor Urnov @urnov.bsky.social · Nov 30

All editors rely on Pol3 because we use its promoters to transcribe the gRNA from plasmid vectors.
I never thought something so cell-essential would be regulated.
-> food for thought wrt editing cell types in various states.

Sheila Teves @sheilateves.bsky.social · Nov 30

🎉Excited to share our new preprint on Biorxiv 🥳
We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress!
So what did we find? 🧵

www.biorxiv.org/content/10.1...

Dynamic regulation of RNA Polymerase III transcription in mouse embryonic stem cells during heat shock stress

Cells respond to many different types of stresses by overhauling gene expression patterns, both at the transcriptional and translational level. Under heat stress, global transcription and translation ...

www.biorxiv.org

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Sheila Teves @sheilateves.bsky.social · Nov 30

Congratulations to recent PhD grad Dr. Thomas Nguyen @thomasfnguyen on wrapping up another research project!! 🎉🎉
Aided by co-authors Dr. James Kwan, grad student Hazel Cui, and undergrad Jennifer Mitchell.
An amazing team!

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