When clinical trials are suddenly halted by US funding cuts, there are repercussions for investigators and patients, and they do not stop at borders.

Koo et al. apply genome-scale double-CRISPRi to map cell envelope gene interactions in Bacillus subtilis, revealing >1,000 genetic interactions and uncovering gene networks in envelope biogenesis and ...

Vibrio cholerae colonizes estuarine environments and human hosts, where it transitions between motile and sessile states using the second messenger cyclic di-GMP (cdG). cdG levels change in response t...

Genomes contain orders of magnitude more open reading frames (ORFs) than known protein coding genes, and recent work suggests there may be unannotated proteins present in even the best studied organisms. To address this gap, we used a high throughput reverse genetic toolkit to construct precise C-terminal fusions of a reporter (and control) to >120,000 ORFs in model E. coli . We found hundreds of unannotated significant hits, and individually detected >50 novel polypeptides by western blot, including ORFs within tRNA loci. Many ORFs overlap annotated genes in the sense orientation, and we found these are likely chimeric polypeptides produced by ribosomal frameshifting. Using degron based knockdowns, we identified unannotated proteins that have putative fitness effects, and we found a novel small protein that displays phenotypes consistent with a role in the mRNA degradosome. The observation of a range of unannotated translation products should lead to better annotation and understanding of the bacterial domain of life and motivates the continued exploration of genomes broadly. ### Competing Interest Statement The authors have declared no competing interest.

Abstract. Targeted gene editing can be achieved using CRISPR–Cas9-assisted recombineering. However, high-efficiency editing requires careful optimization f

Effective delivery of antigens to the immune system is essential for activating the adaptive immune system. Synthetic Spore Husk-Encased Lipids (SSHELs) are synthetic bacterial spore-like particles, w...

Human genome resequencing typically involves mapping reads to a reference genome to call variants; however, this approach suffers from both technical and reference biases, leaving many duplicated and ...

Abstract. Symbiont-mediated male killing (MK) is a mechanism that selectively eliminates male offspring, often by disrupting sex-specific developmental pro

In microbial ecosystems, metabolic interactions are key determinants of species’ relative abundance and activity. Given the immense number of possible interactions in microbial communities, their expe...

Information on the Fall Meeting for ASM Washington, D.C. Branch. The meeting will be October 17, 2025 at Howard University.

Department Name / Number: Molecular Biosciences / 0-1210-000 College: College of Arts and Sciences Hiring Salary: Negotiable Appointment Start Date: August 7, 2026 The Department of Molecular Bioscien...

The Azrieli International Visiting PhD Fellowship offers outstanding international PhD candidates the opportunity to conduct short-term research in Israel, fostering academic collaboration and strengt...